• Korean Journal of Veterinary Research
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• v.43 no.2
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• pp.311-314
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• 2003

Pacheco's disease (PD) is a highly contagious, acute disease of psittacines caused by a herpesvirus. Five 56-day-old dead parakeets (Neophema bourkii) were submitted to the National. Veterinary Research & Quarantine Service for diagnosis. At necropsy, principle lesions were seen in the pancreas, which were hard, whitish, nodular and atrophic resulting in distortion of the duodenal loop. Histologically, lymphocytic pancreatitis with eosinophilic intranuclear inclusions syncytium-formed acinar epithelium was prominent. In the brain, marked focal gliosis and perivascular lymphocytic cuffing were observed. On the electron microscopy examination, various forms of viral particles were found in both the nucleus and cytoplasm of acinar epithelium of pancreas, identified as a herpesvirus. These results were suggested that the cases were diagnosed as PD in the parrakeet.

• Korean Journal of Veterinary Research
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• v.43 no.3
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• pp.449-456
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• 2003

The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.

• Korean Journal of Veterinary Research
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• v.48 no.1
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• pp.33-38
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• 2008

In veterinary medicine amitraz has been used as an insecticide to eliminates mites, lice, and ticks in dogs, cats, goats, swine and cattle. The objective of present study was to developed an analytical method using one-step extraction and determination of the amitraz in veterinary drugs by liquid chromatography (LC). The amitraz was analyzed by LC equipped with Waters XTerra RP18 ($4.8{\times}250mm;\;5{\mu}m;\;Waters,\;USA$) analytical column, using 75% acetonitrile (acetonitrile/D.W; 75/25) at 1.0 ml/ min. The UV-VIS detection of amitraz was made at 290 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $80{\sim}120{\mu}g/ml$. The limit of detection was $0.09{\mu}g/ml $ and limit of quantification was $0.27{\mu}g/ml $. The method showed good intra-day precision (CV 0.05~0.09%) and inter-day precision (CV 0.06~0.18%).

• Korean Journal of Veterinary Research
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• v.48 no.1
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• pp.9-16
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• 2008

A method for the quantification of eugenol in the medicinal herb Clove was developed and validated. For preparation of sample solutions clove was dried at $60^{\circ}C$ for 2h and ground by mixer and extracted with 95% ethanol for shaking extraction. The elutes were analyzed by HPLC system included a reversed phase column, a isocratic mobile phase of 60% methanol and PDA detector set at 280 nm. Calibration graphs were linear with very good correlation coefficients ($r^2>0.9999$) from $0.0125~1{\mu}g/ml$. The limit of detection per sample injection ($20{\mu}l$) was $0.81ng/{\mu}l$ and limit of quantification was $2.47ng/{\mu}l$. The method showed good intra-day precision (%RSD 0.08 ~ 0.27%) and inter-day precision (%RSD 0.32 ~ 1.19%).

• Korean Journal of Veterinary Research
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• v.43 no.1
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• pp.121-127
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• 2003

Ornithobacterium rhinotracheale (OR) is a bacterium responsible for a respiratory disease in turkeys and chickens, and has been identified as one of the emerging respiratory bacterial pathogens. Ten cases of four hundred cases submitted to National Veterinary Research & Quarantine Service for diagnosis in 2001 and in 2002 were diagnosed as OR infection. The major clinical signs of chickens infected with OR were respiratory symptoms including sneezing, sniveling, wet eyes, and swelling of the sinus infraorbitalis at 3 to 4 weeks of age. At necropsy, gross lesions were commonly found to foamish, white, and yoghurt-like exudates in the peritonium and abdominal air sacs. Microscopically, epithelial metaplasia and proliferation of air sacs were prominant with accompaning inflammatory reactions characterized by heterophils, fibrins, and bacterial colonization. Ten field isolates were obtained from air sacs and peritonium of these affected chickens, and were identified as OR, resulted from by gram-staining, catalase, oxidase, API NE and API ZYM Kit. In additon, using a previously reported primer targeted to 16S rRNA of ORT, 784bp fragment was successfully amplified from templates extracted from the isolates and a reference strain. This report describes an occurrence of Ornithobacterium rhinotracheale infection in chickens in Korea.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.6
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• pp.879-883
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• 2005

The integral relationship between the occurrence of lymphocyte nuclear pockets (LNPs) and BLV-infection was examined in Holstein-Friesian dairy cattle in Korea. Transmission electron microscope (TEM) was used to detect LNP in peripheral blood lymphocytes. Morphologically, the membranes of LNP were composed of two layers of double nuclear membrane. The full thickness of LNP membranes including inner and outer nuclear membrane was 60 to 70 nm. LNP prevalence was different according to the bovine leukemia virus (BLV) infection status; in BLV-seropositive cattle, LNP prevalence was 48.4% and in BLV-seronegative cattle prevalence was 5.9%. Moreover, even in seropositive animals, leukemic group was the highest at 70% positive among the groups, followed by suspect group (42.4%) and aleukemic group (23.1%). Consequently, the numbers of LNP were increased in proportion to increase of the numbers of leukocytes among BLV-seropositive cattle. The numbers of LNP per lymphocyte were increased in BLVseropositive cattle compared with seronegative cattle. The mean numbers of LNP per 100-lymphocytes were 0.35, 0.77, 1.64 and 4.7 in BLV-seronegative, BLV-seropositive aleukemic, suspect and leukemic groups, respectively. Thus, it is reasonable that LNP test can be used as the one of the diagnostic criteria of BLV infection.

• Korean Journal of Veterinary Research
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• v.39 no.2
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• pp.338-342
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• 1999

Twenty three strains of Escherichia (E) coli O157 : H7 isolated from Korea, Japan, USA were analyzed by pulsed-field gel electrophoresis (PFGE) of XbaI-digested chromosomal DNA and multiplex polymerase chain reaction. Various PFGE patterns of E. coli O157 : H7 were found on the same farm. Most of the E, coli O157 : H7 strains had shiga-like toxin (slt) II gene only (43.5%) or both slt I and slt II genes(30.4%). eaeA gene was highly conserved in the E. coli O157 : H7. There was no correlation between PFGE and slt gene patterns. The results indicate that various genotypes of E. coli O157 : H7 have spread throughout the country and genomic DNA patterns generated by PFGE are highly specific for different strains and have significant value in epidemiologic investigations of infectious disease outbreaks.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.75-83
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• 2005

The purpose of this study was to investigate the antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces. One hundred and forty-seven E. coli isolates showed resistance to tetracycline (95.2%), erythromycin (89.2%), ampicillin (70.1%), streptomycin (59.2%), cephalothin (56.5%), sulfamethoxazole/trimethoprim (53.7%), ciprofloxacin (57.1%), enrofloxacin (59.2%) and norfloxacin (57.1%). The multiple resistance was seen in 144 isolates (97.9%) and the rate of five, six and seven drugs resistance pattern were 20.4%, 18.4% and 16.3%, respectively. Also, the multiple resistance of E. coli to twelve drugs were seen in 1 isolates (0.7%). Fourteen Salmonella spp. showed resistance to ampicillin (50.0%), streptomycin (57.1%), erythromycin (64.3%) and tetracycline (57.1%) and the rate of two and three drugs resistance pattern were 4 isolates (28.6%), respectively. The prevalence of resistant organisms in Korea probably reflects lack of proper antibiotic policy resulting in prolonged and indiscriminate use of antimicrobial agents.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.113-119
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• 2005

Resistance to antibiotics is a problem all over the world, and this problem also is so extended in veterinary fields. Therefore, this study aimed to find out the antibacterial substances from natural medicinal herbs against bovine mastitis pathogens, especially methicillin-resistant Staphylococcus aureus (MRSA). Among seventy two medicinal herbs, Terminaliae chebula extract showed antimicrobial effect against MRSA isolated from mastitis milk in cow and patient in human. However, Terminaliae chebula extract didn't show antibacterial effects on various strains including other contagious and environmental pathogens related with mastitis of dairy cows.

• Korean Journal of Veterinary Service
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• v.38 no.2
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• pp.141-144
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• 2015

An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.