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http://dx.doi.org/10.7853/kjvs.2015.38.2.141

The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea  

Choi, Eun-Jin (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Lee, Hyun-Kyoung (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Lee, Kyoung-Hyun (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
So, Byoung-Jae (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Song, Jae-Young (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Do, Jae-Chul (Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency)
Yang, Seon-Joo (Equine Hospital, Korea Racing Authority)
Lee, Hyun-Chul (Equine Hospital, Korea Racing Authority)
Yang, Young-Jin (Equine Hospital, Korea Racing Authority)
Publication Information
Korean Journal of Veterinary Service / v.38, no.2, 2015 , pp. 141-144 More about this Journal
Abstract
An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author's knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.
Keywords
Equine herpesvirus-4; Korea; Virus isolation; Immunofluorescence assay; Glycoprotein B;
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