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Expression of VP2 of Aquatic Birnavirus GC-1 Isolated from Rockfish (Sebastes schlegeli), Rearing in Seawater in Korea  

Joh, Seong-joon (Avian Disease Division, National Veterinary Research & Quarantine Service)
Sung, Haan-woo (Avian Disease Division, National Veterinary Research & Quarantine Service)
Lee, Yun-jeong (Avian Disease Division, National Veterinary Research & Quarantine Service)
Kim, Jae-hong (Avian Disease Division, National Veterinary Research & Quarantine Service)
Kang, Shien-young (College of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.3, 2003 , pp. 449-456 More about this Journal
Abstract
The VP2 gene of aquatic birnavirus, Korean isolate (GC-1) was cloned and expressed using the baculovirus expression system. The VP2 gene and VP2 partial gene, which contained a neutralizing epitope, were constructed for recombinant transfer vectors, for baculovirus expression. The expressed recombinant proteins were confirmed by indirect immuno fluorescence antibody (IFA), SDS-PAGE and Western blot. The level of expression was checked at regular time using IFA and Western blot. To measure the neutralizing activity of recombinant proteins against GC-1 strain, the antisera against recombinant proteins were produced by using guinea pigs. The result showed that the antisera neutralized the GC-1 strain. However, the neutralizing titer was higher in antisera against the VP2 gene expressed recombinant protein than that of VP2 partial gene recombinant protein.
Keywords
aquatic birnavirus; GC-1; VP2; expression;
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1 Brown, F. The classification and nomenclature of viruses; summary of results of meeting of the Inter-national Committee on Taxonomy of Virus in Sendai.September 1984. Intervirology 1986, 25, 141-143
2 Crane, M. J., Smith, P. H., William, L. M., Hyatt, A. D., Eaton, L. M., Gould, A., Handlinger, J., Kattenbelt, J. and Gudlovs, N. First isolation of an aquatic bimavirus from fanned and wild fish species in Australia. Dis. Aquat. Org. 2000, 43, 1-14
3 Dobos, P. and Roberts, T. E. The molecular biology of infectious pancreatic necrosis virus: a review. Can. J. Microbiol. 1983, 29, 377-384
4 Estay, A., Farias, G., Soler M. and Kuznar, J. Further analysis on the structural proteins of infectious pancreatic necrosis virus. Vims Res. 1990, 15, 85-96
5 Havarstein, L. S., Kalland, K. H., Christie, K. E. and Endresen, C. Sequence of the large double-stranded RNA segment of the Nl strain of infectious pancreatic necrosis virus: a comparison with other Bimaviridae. J. Gen. Virol. 1990, 71, 299-308
6 Heppell, J., Tarrab, E., Berthiaume, L,. Lecomte, J. and Arrella, M. Characterization of the small open reading frame on genome segment A of infectious pancreatic necrosis vims. J. Gen. Virol. 1995, 76, 2091-2096
7 Joh, S. J. Molecular characterization of a Koreanaquatic birnaviral GC-l strain isolated from rockfish(Sebastes schlegeli). 충북대 박사학위 논문, 2003
8 Sato, H., Emoto, E., Kainata, T., Mori, H., Kamei,K., Kitaoka, A., Takano, R., Nakajima. K., Inui, Y.,Kudo, K. and Hara, S. Cloning and expression ofyellowtail ascite virus segment A. Arch. Virol. 1999,144, 1405-1413
9 Summers, M. D. and Smith, G. E. A manual ofmethods for baculovirus vectors and insect cell cultureprocedures. Texas Agricultural Experirnent Station1987, PP.5-8
10 Dobos, P., Hallett, B. J., Kells, D. T. C., Becht, H. and Teninges, D. Biophysical and biochemical characterization of five animal viruses with biseg-mented double-stranded RNA genomes. J. Virol. 1979, 32, 593-605
11 Christie, K. E., Havastein, L. S., Djupvik, H. 0., Ness, S. and Endresen, C. Characterization of a new serotype of infectious pancreatic necrosis virus isolated from Atlantic salmon. Arch. Virol. 1988, 103, 167-177
12 Hah, Y. C., Hong, S. W.. Kim M. H., Fryer, J. L.and Winton, J. R. Isolation of infectious pancreatic necrosis virus from gold fish Carassius auratus and chum salmon Oncorhynchus keta in Korea. Kor. J. Microbiol. 1984, 22, 85-90
13 Wolf, K. Fish Viruses and Fish Viral Diseases, PP.115-157, Comell University Press, Ithaca and London,1988
14 Perez, L., Chiou, P. P. and Leong, J. A. C. Thestructural proteins of infectious pancreatic necrosisvirus are not glycosylated. J. Virol. 1996, 70, 7247-7249
15 Chung, H. K., Lee, S. H., Lee, H. H., Lee, D. S.and Kim, Y. S. Nucleotide sequence analysis of the VP2-NS-VP3 genes of infectious pancreatic necrosis virus DRT strain. Mol. Cells 1994, 4, 349-354
16 Suzuki, S., Asakura, K. and Kiisuda. R. Productionkinetics of antigenicity and serological analysis of viralpolypeptide of yellowtail ascites virus. Fish Pathol.1995, 30, 209-214
17 Lo, C. F., Hong, Y. W., Huang S. Y. and Wang, C.The characteristics of the virus isolated from the gillof clam Meretrix lusoria. Fish Pathol. 1988, 23, 147-154
18 Macdonald, R. and Dobos, P. Identification of theproteins encoded by each genome segment of infectiouspancreatic necrosis virus. Virol. 1981, 114, 414-422
19 Tarrab, E., Berthiaume, L., Grothe, S., O'Connor-McCourt, M., Heppell, J. and Lecomte, J. Evidenceof a major neutralizable confonnational epitope regionon VP2 of infectious pancreatic necrosis virus. J. Gen.Virol. 1995, 76, 551-558
20 Hjalmarsson, A., Carlemalm, E. and Everitt, E.Infectious pancreatic necrosis virus: identification of aVP3-containing ribonucleoprotein core structure andevidence for O-linked glycosylation of the capsidprotein VP2. J. Virol. 1999, 73, 3484-3490
21 Seo, J. J., Heo, G. J. and Lee, C. H. Characterizationof aquatic bimavirus isolated from rockfish Sebastesschlegeli cultured in Korea. Bull. Eur. Ass. FishPathol. 1998, 18, 87-92
22 Isshiki, T., Nagano, T. and Suzuki, S. Infectivity ofaquabimavirus strains to various marine fish species.Dis. Aquat. Org. 2001, 46, 109-114
23 Frost, P., Borsheim, K. and Endresen, C. Analysis of the antibody response in Atlantic salmon against recombinant VP2 of infectious pancreatic necrosis virus (IPNV). Fish Shellfish Immunol. 1998, 8, 447-456