• Journal of Veterinary Science
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• v.22 no.3
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• Journal of the Korean Magnetic Resonance Society
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• v.24 no.1
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• pp.30-37
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• 2020

Quantitative nuclear magnetic resonance (qNMR) has been gaining attention as a purity assessment method. In particular, qNMR is recognized as the primary method to realize the Internal System of Units (SI) in organic analysis. The capability of quantitative analysis is recognized as the beginning of NMR development. NMR signals are proportional to the number of nuclei and qNMR has been used in various fields, such as metabolomics and food and pharmaceutical analysis. However, careful sample preparation and thorough optimization of measurement parameters are required to obtain accurate and reliable results. In this review, quantitative methods used in qNMR are discussed, and the important factors to be considered also introduced. The recent development of qNMR techniques including combination with chromatography and, multidimensional NMR are also presented.

• The Journal of Asian Finance, Economics and Business
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• v.2 no.4
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• pp.5-11
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• 2015

This study is the first to analyze performance of Taiwanese domestic equity funds between January 2009 and October 2014, the period during which quantitative redirected capital flows toward developing economies and the Taiwanese Stock Exchange Weighted Index compounded at approximately 12.9% annually. Adopting methods endorsed by earlier research, we evaluated 15 Taiwanese equity funds' performance relative to market averages using the Sharpe (1966) and Treynor (1965) ratios and Jensen's alpha method (1968). To test market timing proficiency, we applied the Treynor and Mazuy (1966) and Henriksson and Merton (1981) regression analysis methods. Jensen's alpha method (1968) was used to measure fund managers' stock selection skills. Results revealed that funds significantly under-performed Taiwan's average annual market return and demonstrated no exceptional stock-selection skills and market timing proficiency during the era of quantitative easing.

• Analytical Science and Technology
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• v.26 no.1
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• pp.80-85
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• 2013

The sixty nine accident preparedness substances (APS) having high probability of chemical accident are controlled under the Toxic Chemicals Control Act (TCCA). Around the world, there has been a growing interest in the analysis of chemical warfare agent (CWAs). When a chemical accident occurs, it is generally required to detect and identify APS. However, the quantitative analytical data remain limited in Korea. In this study, an analytical method using GC/MS for volatile organic chemicals was established and a quantitative analysis method was studied. The calibration curve for 25 chemicals were obtained and 21 chemicals showed higher coefficient of determination ($r^2$ >0.998).

• Journal of Wetlands Research
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• v.19 no.1
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• pp.112-121
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• 2017

This study proposed the quantitative precipitation estimation method using overlapped area in radar network. For this purpose, the dense rain gauges and radar network are used. As a result, we found a reflectivity bias between two radar located in different area and developed the new quantitative precipitation estimation method using the bias. Estimated radar rainfall from this method showed the apt radar rainfall estimate than the other results from conventional method at overall rainfall field.

• Korean Journal of Pharmacognosy
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• v.31 no.4
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• pp.416-420
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• 2000

Ursolic acid was isolated from Prunellae Herba (Prunella vulgaris var. lilacina) and identified by direct comparison with an authentic sample. A method of analysis for the evaluation of ursolic acid was developed based on extraction of ground plant material, followed by quantitative determination using capillary gas chromatography of the TMS derivative. Quantitative analysis by GC after derivatisation under mild silylating conditions showed 0.31% ursolic acid in 20 samples collected throughout regions of Korea while no ursolic acid was detected in the samples of the whole plant of Thesium chinense, a substitute for Prunellae Herba in southern regions of Korean peninsula.

• The Journal of the Korea Contents Association
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• v.17 no.4
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• pp.541-549
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• 2017

This research investigates the quantitative screening methods for the Grant Funding system and seeks for the efficient evaluation of a number of proposals. We search foreign cases of Grand Funding, but we found no appropriate model for using in Korea. Thus, we had to develope our own model for better screening. First, we analyse the existing evaluation system and find some problems and challenges. Second, we suggest a quantitative screening system for Grant Funding with a numeric model, and operates a tedious simulation by using the previous data and our suggested model. Third, we test the suggested model and find the optimal model by using simulation method The number of data analysed for simulation is larger than 200 thousands. Last, we suggest some brief policy implications based on the results in the paper.

• Journal of the Korean Society of Tobacco Science
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• v.31 no.2
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• pp.95-106
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• 2009

Semiquantitative analysis by ICP-MS has proven to be a powerful tool for fast screening, in addition, it does not require the element of interest to be present in the calibration standard, making it especially useful for the analysis of unknown samples. In this study, seven cigarette samples were analyzed by the rapid semi-quantitative analysis method based on the ICP-MS. For each cigarette sample, cut tobacco, cigarette paper, filter (before and after smoking), and smoke condensate were analyzed. The accuracy of the analysis technique was evaluated by comparing results obtained from Calibration Check Standard(CCS) and calibration method. Relative Percentage Error(RPE) value of all elements measured for three CCS showed a stable result of less than ${\pm}20%$. Compared to full quantitative analysis by calibration method, the results for cigarette samples showed average error within ${\pm}15%$.

• Nuclear Engineering and Technology
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• v.34 no.4
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• pp.331-343
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• 2002

We performed an importance analysis of In-Service Testing (157) components for Ulchin Unit 3 using the integrated evaluation method for categorizing component safety significance developed in this study. The developed method is basically aimed at having a PSA expert perform an importance analysis using PSA and its related information. The importance analysis using the developed method is initiated by ranking the component importance using quantitative PSA information. The importance analysis of the IST components not modeled in the PSA is performed through the engineering judgment, based on the expertise of PSA, and the quantitative and qualitative information for the 157 components. The PSA scope for importance analysis includes not only Level 1 and 2 internal PSA but also Level 1 external and shutdown/low power operation PSA. The importance analysis results of valves show that 167 (26.55%) of the 629 IST valves are HSSCs and 462 (73.45%) are LSSCs. Those of pumps also show that 28 (70%)of the 40157 pumps are HSSCs and 12 (30%) are LSSCs.

• Korean Journal of Applied Biomechanics
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• v.31 no.2
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• pp.87-94
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• 2021

Objective: Identification of meaningful patterns and trends in large volumes of unstructured data is an important task in various research areas. In the present study, we gathered golf swing image data and did quantitative analysis of swing image. Method: We collected golf swing images of 30 novice players and 30 professional players in this study. Results: We selected important features of swing posture and employed data mining algorithm to classify whether a player is an expert or a novice. Moreover, our proposed method could offer quantitative advices for golf beginners for correcting their swing. Conclusion: Finally, we found a possibility that our proposed method can be expanded to golf swing correction system