• Journal of Applied Biological Chemistry
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• 제66권
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• pp.67-72
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• 2023

A simple and accurate method was developed for the quantitative analysis of oleanonic acid (OA) from mastic gum. The analysis was carried out using reverse-phase high-performance liquid chromatography combined with a photodiode array detector (HPLC/PDA). Our optimized method was validated by measuring various parameters, using an INNO C18 column fitted with a gradient elution system. The results revealed limits of detection and quantification of 0.34 and 1.042 ㎍/mL, respectively. The OA calibration curve exhibited excellent linearity over the concentration range of 0.0625 to 2.0 mg/mL, with r² =0.9996. Accuracy tests revealed a high recovery rate of 99.44-103.66%, with precision values below 0.15%. These results suggest that the present analytical method can identify and quantify OA in mastic gum with high precision. The HPLC approach developed in this study might be applied to routine analyses and large-scale extraction procedures for OA content quantification.

• Journal of the Korean Data and Information Science Society
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• 제8권2호
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• pp.173-181
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• 1997

한의학에서 환자의 병적상태를 분석하고 진단하기위한 전문가시스템은 진단과정에서 환자로부터 받아들인 지식에 기초하므로 지식은 정확하게 표현되어야 한다. 그러나 대부분의 한의진단과정에서 환자로부터 받아들인 지식은 주로 질적자료에 기초하여 기준이 애매한 개념의 지식을 많이 포함하므로 이에 대한 처리가 요구된다. 본 논문에서는 한의전문가시스템 구축에 이용되는 정보의 정확도를 높이기 위하여 Hayashi의 수량화II류를 기반으로 하여 한의진단과정에서 얻어지는 지식을 수량화하는 방법을 제안하였다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.118-121
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• 2003

A method for detection and quantification of aceticlastic methanogens using a real-time PCR with a TaqMan probe was developed. Two sets of primers and probes targeting the family Methanosarcinaceae and Methanosaetaceae were designed by using the Ribosormal Database Project (RDP) II, and softwares for phylogenetic probe design and sequence analysis. Target-group specificity of each set of primers and probe was verified by testing DNAs isolated from pure cultures of 28 archaeal strains purchased from DSMZ. Cell numbers in the 28 archaeal cultures and in the samples from anaerobic processes were quantified using a real-time PCR with the sets of primers and probe. In conclusion, the real-time PCR assay was very specific for the corresponding target methanogenic family and was proved to be a powerful method for quantification of aceticlastic methanogens in anaerobic processes.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2007년도 정기학술발표대회 논문집
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• pp.327-330
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• 2007

기존의 시뮬레이션 일정기법은 최종공사기간(Project Completion Times: PCTs)이 정규분포를 따른다는 가정을 전제로 한다. 그러나 본 논문에서는 이 가정이 항상 옳은 것이 아니며, 이것이 잘못된 결과를 초래할 수 있다는 것을 검증한다. 이처럼 의문이 제기되지 않고 받아들여져 온 가정이 시뮬레이션 분석 결과에 어떠한 영향을 줄 수 있는지를 밝혀내는 리키스 정량화기법(risk Quantification method)을 MATLAB 알고리즘으로 구현하였으며, 네트워크의 모델링에서부터 시뮬레이션 출력 값들로 구성된 샘플집단들에 대한 분석에 이르기까지 전 단계를 MATLAB 프로그래밍으로 구현된 알고리즘을 사용하여 제기된 의문에 대한 답을 제시하였다. 특정 네트워크를 구성하는 엑티비티 기간 값들을 정의하는 확률분포함수의 종류를 다양하게 변화시켜 시뮬레이션 결과 값들 - 최종공사기간 값들 - 을 생성하고, 이처럼 생성된 시뮬레이션 출력 값들로 구성된 샘플집단들의 확률 통계적 특성을 분석하였다. 본 연구는 시뮬레이션을 기반으로 하는 일정관리기법의 신뢰성을 향상시키며, 일정관련 리시크 분석의 정확성을 향상시키는데 기여할 것이다.

• BMB Reports
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• 제43권7호
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• pp.474-479
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• 2010

Three assay methods for quantification of the two galactose-operon mRNAs that only differ by 5 bases in their 5'-end are presented. The 5' ends of each mRNA were extended by ligating the 3'-end of the abundant 5S rRNA. This ligation extends the 5' ends of the two gal mRNAs long enough to be distinguished by the specific PCR primers in the following quantification reactions. Quantification of the corresponding cDNAs was performed either by primer extension assay or real-time qPCR. To circumvent the problem of the RNA ligation reaction (i.e. very low ligation efficiency), we devised a new method that employs real-time qPCR directly for the quantification of the gal transcripts which differ by 5 bases in their 5'-ends.

• Mass Spectrometry Letters
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• 제5권2호
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• pp.52-56
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• 2014

Glycated hemoglobin (HbA1c) is used as an index of mean glycemia over prolonged periods. This study describes an optimization of enzyme digestion conditions for quantification of non-glycated hemoglobin (HbA0) and HbA1c as diagnostic markers of diabetes mellitus. Both HbA0 and HbA1c were quantitatively determined followed by enzyme digestion using isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) with synthesized N-terminal hexapeptides as standards and synthesized isotope labeled hexapeptides as internal standards. Prior to quantification, each peptide was additionally quantified by amino acid composition analysis using ID-LC-MS/MS via acid hydrolysis. Each parameter was considered strictly as a means to improve digestion efficiency and repeatability. Digestion of hemoglobin was optimized when using 100 mM ammonium acetate (pH 4.2) and a Glu-C-to-HbA1c ratio of 1:50 at $37^{\circ}C$ for 20 h. Quantification was satisfactorily reproducible with a 2.6% relative standard deviation. These conditions were recommended for a primary reference method of HbA1c quantification and for the certification of HbA1c reference material.

• Nuclear Engineering and Technology
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• 제46권4호
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• pp.481-488
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• 2014

An analysis of the uncertainty quantification related to LBLOCA using the Monte-Carlo calculation has been performed and compared with the tolerance level determined by the Wilks' formula. The uncertainty range and distribution of each input parameter associated with the LOCA phenomena were determined based on previous PIRT results and documentation during the BEMUSE project. Calulations were conducted on 3,500 cases within a 2-week CPU time on a 14-PC cluster system. The Monte-Carlo exercise shows that the 95% upper limit PCT value can be obtained well, with a 95% confidence level using the Wilks' formula, although we have to endure a 5% risk of PCT under-prediction. The results also show that the statistical fluctuation of the limit value using Wilks' first-order is as large as the uncertainty value itself. It is therefore desirable to increase the order of the Wilks' formula to be higher than the second-order to estimate the reliable safety margin of the design features. It is also shown that, with its ever increasing computational capability, the Monte-Carlo method is accessible for a nuclear power plant safety analysis within a realistic time frame.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.285-290
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• 2021

Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases. Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids in S. herbacea that are known to exert various pharmacological activities. Therefore, our study sought to validate and optimize an HPLC/UV-based analytical method for I3G and Q3G yield quantification, as well as to determine its limit of detection, limit of quantification, linearity, precision, and accuracy. Upon testing a concentration range of 31.5-1.9 ㎍/mL the results exhibited good linearity (r² ≥0.9996 and r² ≥0.9999 for I3G and Q3G, respectively), and the procedure was deemed precise (relative standard deviation of ≤3.19 and ≤3.85%, respectively), and accurate (102.6-105.0 and 92.9-95.2%, respectively). The results showed that our proposed method could be used for rapid I3G and Q3G evaluation in S. herbacea.

• 한국식품영양학회지
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• 제35권6호
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• pp.543-551
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• 2022

There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R²≥0.99). The limits of detection were 0.040~0.765 ㎍/100 g and the limits of quantification were 0.114~1.532 ㎍/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 ㎍/100 g, 78.965 ㎍/100 g, and 165.521 ㎍/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.

• 한국전자통신학회논문지
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• 제17권4호
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• pp.723-728
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• 2022

본 연구는 운전자의 위험운전행동을 평가하고 정량화한 방법에 관한 것이다. 정량화 방법은 차량의 운행을 시작 이후 휴식 없이 연속적으로 주행한 시간, 과속, 급가속, 과속주행 시간 등 다양한 주행정보를 실시간으로 산출하도록 한다. 이러한 정량화된 위험운전행동 값은 개별로 안전운전지수로 제공하거나, 도로를 운행하는 운전자에 대하여 집단에 대한 평가 혹은 차량군에 대한 평가 등으로 객관화하는데 사용할 수 있다.