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http://dx.doi.org/10.9799/ksfan.2022.35.6.543

Development of Simultaneous Analytical Method for Five Lignans in Edible Seeds  

Yoonjeong, Kim (Dept. of Food Science & Biotechnology, Kyungsung University)
Jiye, Pyeon (Dept. of Food Science & Biotechnology, Kyungsung University)
In-hwan, Baek (College of Pharmacy, Kyungsung University)
Younghwa, Kim (Dept. of Food Science & Biotechnology, Kyungsung University)
Publication Information
The Korean Journal of Food And Nutrition / v.35, no.6, 2022 , pp. 543-551 More about this Journal
Abstract
There has been increased interest in lignans due to their potential effect in reducing the risk of developing several diseases. To evaluate lignan contents, sensitive and accurate methods should be developed for their quantification in food. The present study aimed to validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of 5 lignans: lariciresinol (Lar), matairesinol (Mat), pinoresinol (Pin), secoisolariciresinol (Seco), and syringaresinol (Syr). The validation included selectivity, linearity, recovery, accuracy, and precision. The method was proved to be specific, with a linear response (R2≥0.99). The limits of detection were 0.040~0.765 ㎍/100 g and the limits of quantification were 0.114~1.532 ㎍/100 g. Recoveries were 90.588~109.053% for black sesame powder. Relative standard deviations of repeatability and reproducibility were below 5%. Total lignan contents of roasted coffee bean, oat, and blacksoy bean were 105.702 ㎍/100 g, 78.965 ㎍/100 g, and 165.521 ㎍/100 g, respectively. These results showed that LC-MS/MS analysis would be effective in producing acceptable sensitivity, accuracy, and precision in five lignan analyses.
Keywords
lignan; LC-MS/MS; method validation; edible seed;
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