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http://dx.doi.org/10.3839/jabc.2021.039

Validation of an HPLC/UV-based method for Salicornia herbacea-derived isorhamnetin-3-O-glucoside and quercetin-3-O-glucoside quantification  

Park, Jun Yeon (Department of Food Science and Biotechnology, Kyonggi University)
Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University)
Kang, Ki Sung (College of Korean Medicine, Gachon University)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Journal of Applied Biological Chemistry / v.64, no.3, 2021 , pp. 285-290 More about this Journal
Abstract
Salicornia herbacea is a type of salt marsh plant that has been used in traditional medicine to treat several diseases. Isorhamnetin-3-O-glucoside (I3G) and quercetin-3-O-glucoside (Q3G) are major flavonoids in S. herbacea that are known to exert various pharmacological activities. Therefore, our study sought to validate and optimize an HPLC/UV-based analytical method for I3G and Q3G yield quantification, as well as to determine its limit of detection, limit of quantification, linearity, precision, and accuracy. Upon testing a concentration range of 31.5-1.9 ㎍/mL the results exhibited good linearity (r2 ≥0.9996 and r2 ≥0.9999 for I3G and Q3G, respectively), and the procedure was deemed precise (relative standard deviation of ≤3.19 and ≤3.85%, respectively), and accurate (102.6-105.0 and 92.9-95.2%, respectively). The results showed that our proposed method could be used for rapid I3G and Q3G evaluation in S. herbacea.
Keywords
Isorhamnetin-3-O-glucoside; Method validation; Quercetin-3-O-glucoside; Salicornia herbacea;
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