• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.326-331
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• 2008

This study was carried out to analyze the characteristics of each factors by using the quantification theory(I) for prediction of landslide hazard area. The results obtained from this study were summarized as follows; The stepwise regression analysis between landslide sediment ($m^3$ ) and environmental factors, factors affecting landslide sediment ($m^3$ ) were high in order of mixed (forest type), < 15 cm(soil depth), 801~1,200 m (altitude), $31{\sim}40^{\circ}$ (slope gradient), 46 cm < (soil depth), 1,201 m < (altitude) and s(aspect). According to the range, it was shown in order of soil depth (0.3784), altitude (0.2876), forest type (0.2409), slope gradient (0.1728) and aspect (0.1681). The prediction of landslide hazard area was estimated by score table of each category. The extent of prediction score was 0 to 1.2478, and middle score was 0.6239. Class I was over 1.1720, class II was 0.7543 to 0.1719, class III was 0.4989 to 0.7542 and class IV was below 0.4988.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.750-753
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• 2001

Proteomics is a formalized approach for obtaining a rapid snap-shot of the protein complement of a tissue, cell or cell component. Such an approach is powerful in that it allows a parallel assessment of temporal protein fluxes. This is an important concept in view of the dynamic nature of protein expression. Undoubtedly, changes in protein expression are essential in any study aimed at investigating cellular networks. In this study, we analyzed and compared the proteomes of recombinant E. coli strain before and after induction. Proteome expression patterns of recombinant E. coli were resolved on 2D-gels, and the variations in the relative expression level of particular proteins were examined using software-aided protein quantification tool. We observed above 800 spots on a 2D-gel using Melanie II software. Many proteins which involved in chaperones were significantly up-regulated in recombinant E. coli. Therefore, it could be concluded that the expression of recombinant protein in E. coli acted as a stress to the cells, which change cells ability to synthesize proteins and induced the expression of various protective proteins.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.5
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• pp.501-507
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• 2014

Norovirus (NoV) is a major cause of food poisoning outbreaks in Korea. Most NoV outbreaks originate from environmental contamination, but bivalves such as oysters are also important vectors. Oyster Crassostrea gigas contamination by NoV has been reported in Korea, but no quantitative analyses of NoV have been performed. We investigated the NoV concentration in 21 oyster samples from a Korean commercial oyster-growing area with confirmed fecal contamination from January to December 2012, using real-time reverse transcription-polymerase chain reaction. Additionally, we assessed the NoV concentration after heating to investigate the effects of heat treatment on NoV-infected oysters. In NoV-positive samples, the cycle threshold (Ct) values were 37.43-39.41 and 36.77-39.30, while viral concentrations were $8.97{\times}10^2-2.24{\times}10^2$ and $3.05{\times}10^2-7.47{\times}10^1$ copies/g for genogroups I and II, respectively. After heat treatment, NoV genogroup I decreased by 83.4%, 88.0%, 89.4% and 100% at $60^{\circ}C$, $68^{\circ}C$, $70^{\circ}C$, and $100^{\circ}C$, respectively, for 15 min, while genogroup II respectively decreased by 67.3%, 76.3%, 80.1%, and 89.8% under the same conditions.

• Korean Journal of Pharmacognosy
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• v.49 no.3
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• pp.270-277
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• 2018

Pyungwi-san has been used to treat the digestive system diseases, physconia, nausea, anorexia, and dyspepsia in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was optimized for simultaneous determination of the 14 marker components, spinosin, liquiritirn apioside, liquiritin, narirutin, 6'''-feruloylspinosin, hesperidin, liquiritigenin, glycyrrhizin, 6-gingerol, atractylenolide III, honokiol, atractylenolide II, magnolol, and atractylenolide I in Pyungwi-san extract. All analytes were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) with maintained at $45^{\circ}C$. The mobile phase consisted of 0.1% (v/v) aqueous formic acid and acetonitrile. The MS conditions were as follows: capillary voltage 3.3 kV, extractor voltage 3.0 V, RF lens voltage 0.3 V, source temperature $120^{\circ}C$, desolvation temperature $300^{\circ}C$, desolvation gas 600 L/h, cone gas 50 L/h and collision gas 0.14 mL/min. The coefficient of determination of 14 analytes was 0.9989-1.0000. The limits of detection and quantification values of the all analytes were 0.04-2.56 and 0.13-7.69 ng/mL, respectively. As a result of the analysis using the established LC-ESI-MS/MS method, the 5 components, spinosin, 6'''-feruloylspinosin, atractylenolide III, II, and I derived from Zizyphi Fructus and Atractylodis Rhizoma, were not detected in this extract. On the other hand, the 9 components except for the 5 components were 4.15-498.87 mg/kg in lyophilized Pyungwi-san extract. Among these components, glycyrrhizin, marker compound of Glycyrrhizae Radix et Rhizoma, was detected the most amount as a 498.87 mg/kg.

• Journal of Korean Society of Transportation
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• v.22 no.7 s.78
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• pp.33-40
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• 2004

This study aims to provide guidelines for the selection of on-street parking spot on local streets considering conditions of surrounding area and characteristics of traffic generation. This guideline provides the method which determine required roadway width for planning and design of local streets. It is necessary to identify factors for the location selection analysis. This research team selects 12 case study areas to investigate traffic environment on the sites for this analysis. Most of factors which influence on-street parking are found to have a qualitative data format except traffic volume and pedestrian movement data. Quantification theory II which is known to be suitable for qualitative analysis has been applied to identify the meaningful variables for dependent variable. In addition, discriminant analysis has performed to verify the correlation for each variable with hit ratio. Road width, traffic volume, street traders and their heavy packages, and illegally parked vehicle are found to be most significant factors for selection of on-street parking location. Therefore, it is necessary to consider traffic volume generated from massive residential complex and traffic volume for outside and above-mentioned factors for installation of on-street parking facility in the case of new road construction or road width widen.

• Natural Product Sciences
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• v.22 no.2
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• pp.93-101
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• 2016

For efficient quality control of the Samryeongbaekchul-san decoction, a powerful and accurate an ultra-performance liquid chromatography (UPLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS) method was developed for quantitative analysis of the thirteen constituents: allantoin (1), spinosin (2), liquiritin (3), ginsenoside Rg1 (4), liquiritigenin (5), platycodin D2 (6), platycodin D (7), ginsenoside Rb1 (8), glycyrrhizin (9), 6-gingerol (10), atractylenolide III (11), atractylenolide II (12), and atractylenolide I (13). Separation of the compounds 1 - 13 was performed on a UPLC BEH $C_{18}$ column ($2.1{\times}100mm$, $1.7{\mu}m$) at a column temperature of $40^{\circ}C$ with a gradient solvent system of 0.1% (v/v) formic acid aqueous-acetonitrile. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}L$. Calibration curves of all compounds were showed good linearity with values of the correlation coefficient ${\geq}0.9920$ within the test ranges. The values of limits of detection and quantification for all analytes were 0.04 - 4.53 ng/mL and 0.13 - 13.60 ng/mL. The result of an experiment, compounds 2, 6, 12, and 13 were not detected while compounds 1, 3 - 5, and 7 - 11 were detected with 1,570.42, 5,239.85, 299.35, 318.88, 562.27, 340.87, 12,253.69, 73.80, and $115.01{\mu}g/g$, respectively.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.233-239
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• 2014

This study was carried out to propose the criteria for the prediction of landslide occurrence through analysis the influence of each factor by using the quantification theory. The results obtained from this study are summarized as follows. From a stepwise regression analysis between the landslide area($m^2$) and environmental factors, the factors strongly affecting the landslide sediment($m^2$) were the Parents rock (igneous), cross slope(complex), coniferous forests (forest type) and slope gradient ($21{\sim}30^{\circ}$). According to the range, it was shown in order of Cross slope (0.2922), Parents rock (0.2691), Forest type (0.2631) and Slope gradient (0.2312). The range of prediction score of landslide occurrence has been distributed between score 0 and score 1.0556, the median value was score 0.5278. The prediction for class I was over 0.7818, for class II was 0.5279 to 0.7917, for class III 0.2694 to 0.5278 and for class IV was below 0.2693. The prediction on landslide occurrence appeared relatively high accuracy rate as 72% for class I and II. Therefore, this score table for landslide will be very useful for judgement of dangerous slope.

• International Journal of Highway Engineering
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• v.13 no.3
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• pp.157-165
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• 2011

With respect to the trend of highway traffic accident, highway accident is in decline, whileas, the fatality is on an increasing trend. Thus, many efforts to decrease highway traffic accidents and improve the safety, are required. In particular, in case of highway, the management standard by grade for accident black spot is designated. Thus, investing the effect factors by grade for highway traffic accident is required in detail. Thus, in this study, the factors affecting the traffic accidents among the environmental factors based on the graded data for the accident black spot in the applicable section targeting the Seoul-Pusan Express Highway, were reviewed; accident forecasting model which would analyze the characteristics of the accidents for determining the accident grade, was developed. As a result of establishing a model by using Quantification Theory of Type II, considering the characteristics of the dependent and independent variables based on the geometric structure, 'the fixed variable' among the variables relating to the accident, for the variables influencing over the accident grade, 'the type of vans, a chassis and people', 'the trailers, special vehicles and chassis people' and 'the negligence of watching and cloudy weather' were analyzed as common factors, in case of 'horizontal alignment', 'longitudinal slope' and, 'barricade' respectively.

• Korean Journal of Pharmacognosy
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• v.50 no.1
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• pp.65-71
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• 2019

One of the oriental medicine prescriptions, Sagunja-tang consists of four herbal medicines (Ginseng Radix, Poria Sclerotium, Atractylodis Rhizoma Alba, and Glycyrrhiziae Radix et Rhizoma) and has been used as a medicine to enhance tonify the function of spleen and stomach in Korea. In this study, we conducted simultaneous analysis of the 9 marker components, liquiritin apioside, liquiritin, ginsenoside Rg1, liquiritigenin, ginsenoside Rb1, glycyrrhizin, atractylenolide III, atractylenolide II, and atractylenolide I in Sagunja-tang using a liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). Marker compounds were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, 1.7 mm) and the column was maintained at $45^{\circ}C$. The mobile phase consists of 0.1% (v/v) aqueous formic acid and acetonitrile with gradient condition. The LC-MS analysis was performed using a Waters ACQUITY TQD LC-MS/MS system with multiple reaction monitoring (MRM) method in the positive and negative modes. The calibration curves of the nine marker components showed good linearity with coefficient of determination ${\geq}0.9984$ within tested range. The limits of detection and limits of quantification values were 0.27-2.42 ng/mL and 0.81-7.27 ng/mL, respectively. The concentrations of tested 9 analytes in the lyophilized Sagunja-tang sample using the established LC-ESI-MS/MS MRM method were detected up to 16.593 mg/g. These results can be useful as a basic data for the quality control of an oriental medicine prescriptions.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.1
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• pp.45-53
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• 2019

As the urbanization progresses, the demand for parking in large cities surges compared with that for parking lots. However, the space for securing a parking lot in a large city is physically limited and a budget problem also arises. Therefore, a shared parking system that can utilize existing parking lots is becoming important. This study was carried out to analyze the parking area efficiently for the residents parking area. As a result of the questionnaire survey, it was the most frequent passage to work / commuting and business purposes. The most important factors in parking were 'Parking charge', 'Walking distance to the destination after parking', 'Parking lot searching time' Approximately 46 % of the respondents were female. As a result of the quantitative analysis of the factors influencing the potential use of the intention to use as a dependent variable, it was analyzed that the policy was effective to reduce the parking fee to less than 500 won per 10 minutes and take about 3-6 minutes to search.