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Quantitative Determination of the Marker Components in Pyungwi-San Using LC-ESI-MS/MS  

Seo, Chang-Seob (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Pharmacognosy / v.49, no.3, 2018 , pp. 270-277 More about this Journal
Abstract
Pyungwi-san has been used to treat the digestive system diseases, physconia, nausea, anorexia, and dyspepsia in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS/MS) method was optimized for simultaneous determination of the 14 marker components, spinosin, liquiritirn apioside, liquiritin, narirutin, 6'''-feruloylspinosin, hesperidin, liquiritigenin, glycyrrhizin, 6-gingerol, atractylenolide III, honokiol, atractylenolide II, magnolol, and atractylenolide I in Pyungwi-san extract. All analytes were separated on a Waters Acquity UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}m$) with maintained at $45^{\circ}C$. The mobile phase consisted of 0.1% (v/v) aqueous formic acid and acetonitrile. The MS conditions were as follows: capillary voltage 3.3 kV, extractor voltage 3.0 V, RF lens voltage 0.3 V, source temperature $120^{\circ}C$, desolvation temperature $300^{\circ}C$, desolvation gas 600 L/h, cone gas 50 L/h and collision gas 0.14 mL/min. The coefficient of determination of 14 analytes was 0.9989-1.0000. The limits of detection and quantification values of the all analytes were 0.04-2.56 and 0.13-7.69 ng/mL, respectively. As a result of the analysis using the established LC-ESI-MS/MS method, the 5 components, spinosin, 6'''-feruloylspinosin, atractylenolide III, II, and I derived from Zizyphi Fructus and Atractylodis Rhizoma, were not detected in this extract. On the other hand, the 9 components except for the 5 components were 4.15-498.87 mg/kg in lyophilized Pyungwi-san extract. Among these components, glycyrrhizin, marker compound of Glycyrrhizae Radix et Rhizoma, was detected the most amount as a 498.87 mg/kg.
Keywords
Pyungwi-san; Quantitative determination; LC-ESI-MS/MS;
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1 Seo, C. S., Kim, J. H., Lim, S. H. and Shin, H. K. (2011) Estimation of shelf-life by long-term storage test of Pyungwi-san. Korean J. Orient. Med. Prescr. 19: 183-194.
2 Jin, S. E., Jeong, S. J., Shin, H. K. and Ha, H. (2013) Hepatoprotective effect of 25 herbal formulas in primary rat hepatocytes. Korean J. Oriental Physiol. Pathol. 27: 617-624.
3 Lee, M. C., Park, J. R., Shim, J. H., Ahn, T. S. and Kim, B. J. (2015) Effect of traditional Chinese herbal medicine Shengmai-san and Pyungwi-san on gastrointestinal motility in mice. J. Korean Med. Obes. Res. 15: 68-74.   DOI
4 Seo, C. S., Lee, J. A., Jung, D., Lee, H. Y., Lee, J. K., Ha, H., Lee, M. Y. and Shin, H. K. (2011) Simultaneous determination of liquiritin, hesperidin, and glycyrrhizin by HPLC-photodiode array detection and the anti-inflammatory effect of Pyungwi-san. Arch. Pharm. Res. 34: 203-210.   DOI
5 Ha, H., Shin, I. S., Lim, H. S., Jeon, W. Y., Kim, J. H., Seo, C. S. and Shin, H. K. (2012) Change in anti-inflammatory effect of Pyungwi-san decoction according to the preservation temperature and period. Herb. Formula Sci. 20: 29-35.
6 Lee, H. S. and Moon, J. Y. (2007) Scavenging property of Pyungwi-san herbal acupuncture solution on ROS and RNS. Korean J. Orient. Physiol. Pathol. 21: 165-170.
7 Jang, D. R., Hwang, Y. H., Jung, K. Y., Ha, J. H., Park, H. Y. and Ma, J. Y. (2013) A study on acute oral toxicity of Pyungwi-san and fermented Pyungwi-san in ICR mice. J. Orient. Obstet. Gynecol. 26: 59-68.
8 Shin, I. S., Seo, C. S., Ha, H. K., Lee, M. Y., Huang, D. S., Huh, J. I. and Shin, H. K. (2011) Genotoxicity assessment of Pyungwi-san (PWS), a traditional herbal prescription. J. Ethnopharmacol. 133: 696-703.   DOI
9 Heo, J. (2004) Donguibogam, 150. Namsandang, Seoul.
10 Shin, I. S., Kim, J. H., Ha, H. K., Huang, D. S., Huh, J. I. and Shin, H. K. (2010) Study on safety of Pyungwi-san in Sprague-Dawley rats. Korean J. Oriental Physiol. Pathol. 24: 426-429.
11 Wang, C., Pan, Y., Fan, G., Chai, Y. and Wu, Y. (2010) Application of an efficient strategy based on MAE, HPLC-DAD-MS/MS and HSCCC for the rapid extraction, identification, separation and purification of flavonoids from Fructus Aurantii Immaturus. Biomed. Chromagogr. 24: 235-244.   DOI
12 Ren, Y., Wang, P., Wu, C., Zhang, J. and Niu, C. (2013) Identification of the metabolites after oral administration of extract of Ziziphi Spinosae Semen to rats or dogs by highperformance liquid chromatography/linear ion trap FTICR hybrid mass spectrometry. Biomed. Chromatogr. 27: 17-26.   DOI
13 Li, L., Liang, S., Du, F. and Li, C. (2007) Simultaneous quantification of multiple licorice flavonoids in rat plasma. J. Am. Soc. Mass Spectrom. 18: 778-782.   DOI
14 Tan, G., Zhu, Z., Jing, J., Lv, L., Lou, Z., Zhang, G. and Chai, Y. (2011) Characterization of constituents in Sini decoction and rat plasma by high-performance liquid chromatography with diode array detection coupled to time-of-flight mass spectrometry. Biomed. Chromagogr. 25: 913-924.   DOI
15 Zhou, D., Xu, Q., Xue, X., Zhang, F. and Liang, X. (2006) Identification of O-diglycosyl flavanones in Fructus Aurantii by liquid chromatography with electrospray ionization and collision-induced dissociation mass spectrometry. J. Pharm. Biomed. Anal. 42: 441-448.   DOI
16 Jiang, Y., Vaysse, J., Gilard, V., Balayssac, S., Dejean, S., Malet-Martino, M., David, B., Fiorini, C. and Barbin, Y. (2012) Quality assessment of commercial Magnoliae officinalis cortex by $^1H$-NMR-based metabolomics and HPLC methods. Phytochem. Anal. 23: 387-395.   DOI
17 Yu, Q., Xiang, J., Tang, W. F., Liang, M. Z., Qin, Y. P. and Nan, F. (2009) Simulataneous determination of the 10 major components of Da-Cheng-Qi decoction in dog plasma by liquid chromatography tandem mass spectrometry. J. Chromatogr. B 877: 2025-2031.   DOI
18 Chen, L., Qi, J., Chang, Y., Zhu, D. and Yu, B. (2009) Identification and determination of the major constituents in Traditional Chinese Medicinal formula Danggui-Shaoyao-San by HPLC-DAD-ESI-MS/MS. J. Pharm. Biomed. Anal. 50: 127-137.   DOI
19 Tsai, C. J., Liang, J. W. and Lin, H. R. (2012) Sesquiterpenoids from Atractylodes macrocephala act as farnesoid X receptor and progesterone receptor modulators. Bioorg. Med. Chem. Lett. 22: 2326-2329.   DOI
20 Liu, E. H., Zhao, P., Duan, L., Zheng, G. D., Guo, L., Yang, H. and Li, P. (2013) Simultaneous determination of six bioactive flavonoids in Citri Reticulatae Pericarpium by rapid resolution liquid chromatography coupled with triple quadrupole electrospray tandem mass spectrometry. Food Chem. 141: 3977-3983.   DOI
21 Zick, S. M., Ruffin, M. T., Djuric, Z., Normolle, D. and Brenner, D. E. (2010) Quantitation of 6-, 8- and 10-gingerols and 6-shogaol in human plasma by high-performance liquid chromatography with electrochemical detection. Int. J. Biomed. Sci. 6: 233-240.
22 Niu, C. and Zhang, J. (2011) Quantitative analysis and chromatographic fingerprinting of the Semen Zizyphi Spinosae by ultra-high-performance liquid chromatography coupled with diode-array detector. J. Sep. Sci. 34: 2989-2996.   DOI
23 Zhang, Q. and Ye, M. (2009) Chemical analysis of the Chinese herbal medicine Gan-Cao (licorice). J. Chromatogr. A. 1216: 1954-1969.   DOI
24 Yoon, Y. P. (2008) The guideline on the visual and organoleptic examination of herbal medicine II, 29, 100, 125. Ministry of Food and Drug Safety, Seoul.
25 Moon, C. J. (2006) The guideline on the visual and organoleptic examination of herbal medicine I, 17, 64, 90. Ministry of Food and Drug Safety, Seoul.