We analyzed QTLs for alkali-related digestion by using 120 population crossed Cheongcheong and Nagdong derived from anther culture (CNDH). The DNA markers located in the QTLs gene were selected and applied to existing cultivars. As a result of the investigation of the alkali decay degree, brown rice of Cheongcheong and Nagdong was 1.9 and 1.6, respectively, and the CNDH was $3.79{\pm}2.01$, and the distribution of variance was distributed to 7.0-1.0. The milled rice of Cheongcheong and Nagdong was 5.6 and 4.1, respectively. The mean of the CNDH was $4.86{\pm}1.55$, and the distribution of variance was distributed to 7.0-2.0. Variation distribution curves showed continuous variation that was close to non-normal distribution. In the QTLs analysis, qBRA2, qBRA6, and qBRA11 were mapped in 1-2 replications of brown rice. QHRA2-1, qHRA2-2, qHRA2-3, qHRA3, and qHRA8 were mapped in the first replication. QHRA2-1, qHRA2-2, qHRA2-3 and qHRA3 were mapped in the second replicates. And mapped to qHRA5 in 4 replicates. These were found on chromosome 2, 3, 6, 8 and 11, respectively. The phenotypic variations of qBRA2, qBRA6, and qBRA11 on the chromosomes of brown and milled rice were 1-9%. The polymorphism was analyzed for 12 types of the japonica type and six types of the indica type, based on the nine markers found in the QTLs analysis of alkali digestion. Chromosome 11, RM27258, was selected to determine the segregation ratio, which shows the difference in size by the band pattern. The results of this study will be used as basic data for the development of high-quality rice cultivars.
After Fukushima Daiichi nuclear disaster in 2011, study and maintenance of monitoring systems have been made at home and abroad. As concerns about radioactive contamination of water have increased in Korea, update of maintenance of managing radioactive materials in water is being made mainly by Ministry of Environment. In this study, we analysed current state of monitoring system modification in Japan, the country directly involved and neighboring country. According to the result, Japan modified the legislations first. Then Ministry of Education, Culture, Sports, Science and Technology (MEXT) provides theoretical background of radiological monitoring. And Ministry of the Environment actually watches state of water pollution in public waters and underground water. Finally related agencies like local government are monitoring current state of radioactive contamination in water environment. By region, local monitoring stations share the investigation of the whole country. Also, additional monitoring is running around nuclear facilities. After Fukushima disaster, monitoring for area near Fukushima is added. Among the reference levels, management target value of drinking water and tap water is 10 Bq/kg, and those of public water and underground water are 1 Bq/L. Measuring intervals varied from every hour to once a year, regularly or irregularly depending on the investigation. The main measuring items are air dose rate, gross ${\alpha}$, gross ${\beta}$, ${\gamma}$ radionuclide, Cs-134, Cs-137, Sr-89, Sr-90, I-131, and so on. In comparison, regulations about general public water in Korea need to be modified, while those about area near nuclear facility and drinking water are organized well. In future, therefore, domestic system would be expected to be modified with making reference to the guidelines like WHO's one. As good case of applying international guideline to domestic environment, Japanese system could be a reference when general standard of radioactivity in public water is made in Korea.
A wide variety of methods have been used to control Dental Unit Waterline (DUWL) contamination. Among the methods, flushing is mainly used because it is simple and easy to use. Generally, flushing of DUWL for 20 or 30 sec before using high speed handpieces or scalers is recommended. However, the appropriateness of flushing time was not investigated thoroughly. The purpose of this study was to check the effective time of flushing for decreasing bacterial contamination. Seven dental unit chairs were randomly selected in student clinical simulation laboratory for this experiment. DUWLs were continuously flushed and water samples were collected at an interval of 30 seconds for 15 minutes. From five dental unit chairs, water samples were collected every 10 seconds for 1 minute. Bacterial levels in water samples were examined by the culture method on R2A plates. After 10 second flushing of DUWLs, the number of bacteria significantly reduced and decreased continuously up to 40 seconds. However, even after the water was flushed for 15 minutes, the bacterial contamination level was not reduced below recommended bacteria level, 200 CFU/ml. In addition to flushing, the periodic chemical disinfection is required to control the DUWL water to the recommended level.
This study was carried out in order to investigate the changes in chemical components of four kinds of Cheonggukjang during fermentation. Three different kinds of Cheonggukjang were prepared with germinated soybeans using rice straw, Bacillus natto, Bacillus natto plus Aspergillus oryzae, and non-germinated soybeans using rice straw. The nitrogen contents increased significantly during fermentation in all kinds of Cheonggukjang. Especially germinated soybean Cheonggukjang prepared with A. oryzae plus B. natto showed approximately 1.3 to 3.0 fold higher values than the other Cheonggukjangs. Total free amino acid contents of all Cheonggukjangs increased with an increase in fermentation time until 60 h. Lysine content was highest in Cheonggukjang prepared with ungerminated soybean at 72 h of fermentation. Among free amino acids of germinated soybean Cheonggukjang prepared with rice straw, glutamic acid was highest (3.64 mg/g) after 72 h of fermentation. In Cheonggukjang prepared with mixed culture, glutamic acid content was 4.37 mg/g. Total carbohydrate contents of Cheonggukjang decreased rapidly with an increase in fermentation time except the ungerminated soybean Cheonggukjang, and the total carbohydrate contents varied from 9.81 to 14.04% after 72 h of fermentation. On the other hand, total carbohydrate contents of ungerminated soybean Cheonggukjang prepared with rice straw gradually decreased during fermentation. In conclusion, it is expected to increase the contents of functional constituents and to improve quality characteristics of Cheonggukjang when it is prepared with germinated soybeans using B. natto plus A. oryaze.
This paper introduces a new technique that reduces the search times and Improves the accuracy of motion estimation using high temporal and spatial correlation of motion vector. Instead of using the fixed first search Point of previously proposed search algorithms, the proposed method finds more accurate first search point as to compensating searching area using high temporal and spatial correlation of motion vector. Therefore, the main idea of proposed method is to find first search point to improve the performance of motion estimation and reduce the search times. The proposed method utilizes the direction of the same coordinate block of the previous frame compared with a block of the current frame to use temporal correlation and the direction of the adjacent blocks of the current frame to use spatial correlation. Based on these directions, we compute the first search point. We search the motion vector in the middle of computed first search point with two fixed search patterns. Using that idea, an efficient adaptive predicted direction search algorithm (APDSA) for block matching motion estimation is proposed. In the experimental results show that the PSNR values are improved up to the 3.6dB as depend on the Image sequences and advanced about 1.7dB on an average. The results of the comparison show that the performance of the proposed APDSA algorithm is better than those of other fast search algorithms whether the image sequence contains fast or slow motion, and is similar to the performance of the FS (Full Search) algorithm. Simulation results also show that the performance of the APDSA scheme gives better subjective picture quality than the other fast search algorithms and is closer to that of the FS algorithm.
Eleven runoff $plots(3{\times}15\;m)$ were built on a sloping field of a high plateau in Kangwon Province, Korea. The plots were treated with different tillage, residue covers and fertilizers, corn and potato were cultivated, and sediment discharge was measured from the plots for 3 years. Agricultural management practices were monitored around the plots to develop adequate best management practices. The least sediment occurred from the plots with no-till and 100% residue cover $(corn,\;0.1{\sim}2.2\;t/ha/year)$ and with contour tillage and vinyl sheet cover plots $(potato,\;0.1{\sim}0.2\;t/ha/year)$. The largest sediment was measured from the plots with up-and-down till and no cover ($11{\sim}33$ and $16{\sim}31\;t/ha/year$ from corn and potato plots, respectively). The type of organic and commercial fertilizers seemed not to affect sediment discharge. Sediment discharge from contour plots were largely dependent on the collapse of ridges due to the flush of water stored in furrows. The sediment discharge from contour corn and potato plots with no residue cover was $10{\sim}27$ and $16{\sim}24\;t/ha/year$, respectively. No-till with residue cover, vinyl cover for tuber crops, construction of furrow dam, frost heave research, furrow and ridge tillage with furrow dam after tuber crop harvest, limited stalk harvest of row crops, use of winter cover crops, and other common best management practices were recommended to minimize the sediment discharge from a high plateau sloping land culture.
This study was designed to examine the factors affecting in fertilization and development of embryos in vitro, and to examine whether zone drilling by laser irradiation can improve the hatching rate of IVF embryos from DNA marker-proved Hanwoo. DNA markers related to marbling score were identified using DNA fingerprinting with Ml3 probe and restriction enzyme Hae III. Oocytes were aspirated from immature ovarian follicles using a combined method of rectal ovarian-palpation and transvaginal ultrasound-guidance(6.5MHz) under local anesthesia. The aspirated oocytes were washed twice with fresh D-PBS containing 5% FBS and were rewashed 4 to 5 times with TCM-199 containing 5% FBS. A morphological grade of I to IV was assigned to each oocyte. Data were analyzed using the GLM procedure of SAS. Sperm separation methods did not have any significant effect on cleavage or developmental abilities of IVF embryos. Significantly(P<0.05) higher cleavage rate was observed in embryos from GI(60.0%, 3/5), GII(69.2%, 18/26) and GIII(62.1%, 59/95) compared to embryos from GIV oocytes(36.2%, 25/69). And the developmental rate to blastocyst stage was higher(P<0.05) in embryos from GI(33.3%, 1/3) and GII oocytes(38.9%, 7/18) than those from GIII(16.9%,10/59) and GIV oocytes(4.0%, 1/25). There was no significant difference in development of IVF embryos to blastocyst by media for in vitro culture. Proportion of hatched blastocyst was significantly(P<0.05) higher in embryos received zona drilling by laser than those of non-drilled.
A feeding trial was conducted to study the effects of a live yeast, Pichia farinosa culture(PF), on the production performance and intestinal microflora in laying hens. One hundred and sixty ISA Brown layers, 21 weeks of age, were randomly allotted to four dietary treatments, with four replicates per treatment. Dietary treatments consisted of four levels (0, 0.1, 0.3, and 0.5%) of PF added to a com-soybean meal based diet. Egg production, egg weight, feed intake and fred conversion ratio(FCR) were measured. Egg qualifies were examined at 25th and 29th weeks of age. A metabolism trial was conducted following the feeding trial, during which intestinal microflora, nutrient digestibility and fecal NH3 gas emission were measured. Egg production of birds fed 0.1 and 0.3% PF were significantly higher than those from birds fed 0 and 0.5% PF(P<0.05). Daily egg mass of 0.3% PF increased significantly compared to that of 0% PF. There was no difference in egg weight among all treatments. Feed conversion ratio was significantly improved as the PF level increased. No significant difference was found in eggshell quality and Haugh unit at both 25 and 29 weeks of age. Viable count of ileal Lactobacillus spp. increased significantly as the rf level increased. However, the total number of yeast and anaerobes in ileum were similar among all treatments. Cecal Lactobacillus spp. and yeast counts showed no difference among all treatments. Fecal NH$_{3}$ gas emission of layers fed PF decreased significantly by the PF supplementation. From the result of this experiment, it could be concluded that dietary supplementation of the live yeast Pichia farinosa improves the laying performance and decreases the fecal ammonia gas emission.
Proceedings of the Korean Institute Of Construction Engineering and Management
/
한국건설관리학회 2002년도 학술대회지
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pp.115-122
/
2002
There is various points that should be improved in Fairness such as our contract practice to propose construction projects, project managing and the stakeholders' way of thinking and culture. We consider that the revision of construction related provisions and systems is required but even more, an overall change in business management through the implementation of Integrated Construction Information Management System that will enable the owner, which drives the project, and contractor sharing construction information is required. To mange construction related information in an integrated manner, designing information should be smoothly transferred to purchasing information, and changes are required in order to move ahead to process-oriented work system. Finally information created from various construction organizations should be delivered in an aligned and standardized manner as well. The domestic Nuclear Power Plant Construction has been accepting various technology transfers from U.S, France, Canada and UK, which enabled us to self-support technology and recently even proceeded to the phase exporting our technology to others. However, continuous effort is required to improve internal business efficiency and to respond to external environmental change such aselectricity market deregulation. Recently, in accordance with the result in number of CEO's intention to make progress in IT and improve business efficiency, the number of enterprises introducing Enterprise Resource Planning is increasing. ERP is an innovative tool which changes the way of performing work from organization and department orientation to process-orientation in order to optimize the resources, such as human and material resources, through out the Enterprise by performing BPR which will maximize overall business efficiency of the enterprise, such includes not only construction management, but also business management. KHNP continued to performing large scaled construction projects such as nuclear power plant construction for past 30 years and took the initiatives of large scale project management and Quality management ability in domestic industry by having independent capability of over all construction planning, purchasing and, construction and start up management etc. To maintain our leading position of improving construction management technology based on our accumulated project management experience and technology, KHNP included construction into our ERP project in purpose of innovating construction business. We would like to discuss the characteristics of nuclear construction business, project management system, information system infrastructure and information sharing system among construction related entities, and implementation practices for information system, and consider how to resolve our practice that should be improved in this thesis.
A single phase composter was constructed by modifying the conventional mixer of sawdust for the cultivation of oyster mushroom Pleurotus ostreatus. The machine was designed on the basis of 3-phase-1 system which was controlled in prewetting, pasteurization and fermentation processes. In composting 200 kg of straw and cotton waste in the machine, it took 20 minutes in prewetting step and also to hours at $65^{\circ}C$ in pasteurization process. Postfermentation by aerothermophiles was completed by treating the compost at $45^{\circ}C-50^{\circ}C$ for 48 hours which was shorten 24 hours from the conventional method. In the postfermentation at high temperature, forced aeration and/or vigorous mixing process(es) played a great role in the improvement of spawn quality. The growth of mycelium of oyster mushroom was excellent in the culture combinated with 3 parts of surface inoculation and 7 parts of mechanical mixing.
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