• Asian-Australasian Journal of Animal Sciences
• /
• 제29권9호
• /
• pp.1363-1370
• /
• 2016

Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain could serve as a novel source for rennet production. Yaks total RNA was extracted from the abomasum of an unweaned yak. The yak preprochymosin, prochymosin, and chymosin genes from total RNA were isolated using gene specific primers based on cattle chymosin gene sequence respectively and analyzed their expression pattern byreal time-polymerase chain reaction. The result showed that the chymosin gene expression level of the sucking yaks was 11.45 times higher than one of adult yaks and yak chymosin belongs to Bovidae family in phylogenetic analysis. To express each, the preprochymosin, prochymosin, and chymosin genes were ligated into the expression vector $pPICZ{\alpha}A$, respectively, and were expressed in Pichia pastoris X33. The results showed that all the recombinant clones of P. pastoris containing the preprochymosin, prochymosin or chymosin genes could produce the active form of recombinant chymosin into the culture supernatant. Heterologous expressed prochymosin (14.55 Soxhlet unit/mL) had the highest enzyme activity of the three expressed chymosin enzymes. Therefore, we suggest that the yak chymosin gene recombinant Pichia pastoris strain could provide an alternative source of rennet production.

• Asian-Australasian Journal of Animal Sciences
• /
• 제27권12호
• /
• pp.1684-1690
• /
• 2014

Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone $ER{\alpha}$ and $ER{\beta}$ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of $ER{\alpha}$ and $ER{\beta}$. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak $ER{\alpha}$ and $ER{\beta}$ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that $ER{\alpha}$ and $ER{\beta}$ were expressed in a variety of tissues, but the expression level of $ER{\alpha}$ was higher than that of $ER{\beta}$ in all tissues, except testis. The mRNA expression of $ER{\alpha}$ was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The $ER{\beta}$ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권12호
• /
• pp.1905-1911
• /
• 2020

Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

• Molecules and Cells
• /
• 제39권2호
• /
• pp.96-102
• /
• 2016

miR-101 is considered to play an important role in hepatocellular carcinoma (HCC), but the underlying molecular mechanism remains to be elucidated. Here, we aimed to confirm whether Girdin is a target gene of miR-101 and determine the tumor suppressor of miR-101 through Girdin pathway. In our previous studies, we firstly found Girdin protein was overexpressed in HCC tissues, and it closely correlated to tumor size, T stage, TNM stage and Edmondson-Steiner stage of HCC patients. After specific small interfering RNA of Girdin was transfected into HepG2 and Huh7.5.1 cells, the proliferation and invasion ability of tumor cells were significantly inhibited. In this study, we further explored the detailed molecular mechanism of Girdin in HCC. Interestingly, we found that miR-101 significantly low-expressed in HCC tissues compared with that in matched normal tissues while Girdin had a relative higher expression, and miR-101 was inversely correlated with Girdin expression. In addition, after miR-101 transfection, the proliferation, migration and invasion abilities of HepG2 cells were weakened. Furthermore, we confirmed that Girdin is a direct target gene of miR-101. Finally we confirmed Talen-mediated Girdin knockout markedly suppressed cell proliferation, migration and invasion in HCC while downregulation of miR-101 significantly restored the inhibitory effect. Our findings suggested that miR-101/Girdin axis could be a potential application of HCC treatment.

• Wind and Structures
• /
• 제22권5호
• /
• pp.555-571
• /
• 2016

A hanging-type sand-retaining wall is a very common sand-blocking fence structure used to prevent sand movement. This type of wall is widely used along the Qinghai-Tibet and Gobi desert railways in Xinjiang, Western China. To analyze the characteristics of wind-sand flow fields under the effect of such a sand fence structure, a wind tunnel test and a field test were carried out. The wind tunnel test showed the zoning characteristics of the flow fields under the effect of the hanging-type sand-retaining wall, and the field test provided the sediment transport data for effective wind-proof interval and the sand resistance data in the front and behind the sand-retaining wall. The consistency of the wind-sand flow fields with the spatial distribution characteristic of wind-carried sand motion was verified by the correspondences of the acceleration zone in the flow field and the negative elevation points of the percentage variations of the sand collection rate. The spatial distribution characteristic of the field sand collection data further showed the spatial structural characteristic of the sandy air currents under the action of the hanging-type sand-retaining wall and the sand resistance characteristic of the sand-retaining wall. This systematic study on the wind-sand flow fields under the control of the hanging-type sand-retaining wall provides a theoretical basis for the rational layout of sand control engineering systems and the efficient utilization of a hanging-type sand-retaining wall.

• The Korean Journal of Pain
• /
• 제33권3호
• /
• pp.216-225
• /
• 2020

Background: Garlic oil is a rich source of organosulfur compounds including diallyl disulfide and diallyl trisulfide. There have been studies showing the neuroprotective actions of these organosulfur compounds. However, the potential of these organosulfur compounds in neuropathic pain has not been explored. The present study was aimed at investigating the pain attenuating potential of diallyl disulfide and diallyl trisulfide in chronic constriction injury (CCI)-induced neuropathic pain in rats. The study also explored their pain-attenuating mechanisms through modulation of H₂S, brain-derived neurotrophin factor (BDNF) and nuclear factor erythroid 2-related factor 2 (Nrf2). Methods: The rats were subjected to CCI injury by ligating the sciatic nerve in four places. The development of neuropathic pain was measured by assessing mechanical hyperalgesia (Randall-Selittotest), mechanical allodynia (Von Frey test), and cold allodynia (acetone drop test) on 14th day after surgery. Results: Administration of diallyl disulfide (25 and 50 mg/kg) and diallyl trisulfide (20 and 40 mg/kg) for 14 days led to a significant reduction in pain in CCI-subjected rats. Moreover, treatment with these organosulfur compounds led to the restoration of H₂S, BDNF and Nrf2 levels in the sciatic nerve and dorsal root ganglia. Co-administration of ANA-12 (BDNF blocker) abolished pain attenuating actions as well as BDNF and the Nrf2 restorative actions of diallyl disulfide and diallyl trisulfide, without modulating H₂S levels. Conclusions: Diallyl disulfide and diallyl trisulfide have the potential to attenuate neuropathic pain in CCI-subjected rats possibly through activation of H₂S-BDNF-Nrf2 signaling pathway.

• 국제지역연구
• /
• 제13권3호
• /
• pp.729-757
• /
• 2009

본 연구는 중국의 WTO가입 이후 중국 각 성 시(省 市)가 조직을 잘 운영하고 있는지 가늠해보고, 이를 근거로 향후 어느 정도 개선이 필요한지를 판단하기 위해서 2002년부터 2006년까지의 중국 각 성 시의 데이터를 이용하여 자료포락분석(DEA)기법 중 투입지향CCR모형과 투입지향BCC모형으로 효율성평가를 실시하였다. 투입지향모형을 활용하는 것은 산출보다는 투입부문에서의 절감 등이 더 용이하다고 판단한 것에 기인하며, 일반적으로 지방정부 효율성평가에서 활용되고 있다. 분석결과 상해만이 2002년부터 2006년까지 꾸준히 효율성이 가장 높은 성(省)으로 나타났는데, 다른 성 시들은 발전과 효율성제고를 위해 상해를 벤치마킹할 필요가 있다고 본다. 지리적 문화적 정서적 차이 등이 있겠지만 서장, 산서, 감숙, 청해, 영하 그리고 신강은 WTO가입 이후에도 지속적으로 효율성이 낮은 지역으로 분류되는데, 다양한 소수민족으로 구성되어 있고 지역 간 경제발전의 편차가 심한 중국의 입장에서는 정책수립 시 이들 지역에 대한 경제발전방안 등을 마련하는데 기초 자료로서 자료포락분석(DEA)기법에 의한 효율성평가결과를 활용하는 것이 바람직할 수도 있다.

• Parasites, Hosts and Diseases
• /
• 제60권1호
• /
• pp.25-34
• /
• 2022

Alveolar echinococcosis (AE) caused by infection with E. multilocularis metacestode, represents one of the most fatal helminthic diseases. AE is principally manifested with infiltrative, proliferating hepatic mass, resembling primary hepatocellular carcinoma. Sometimes metastatic lesions are found in nearby or remote tissue. AE diagnosis largely depends on imaging studies, but atypical findings of imaging features frequently require differential diagnosis from other hepatic lesions. Serological tests may provide further evidence, while obtaining reliable AE materials is not easy. In this study, alternative antigens, specific to AE were identified by analyzing E. granulosus protoscolex proteins. An immunoblot analysis of E. granulosus protoscolex showed that a group of low-molecular-weight proteins in the range from 14 kDa to 16 kDa exhibited a sensitive and specific immune response to AE patient sera. Partial purification and proteomic analysis indicated that this protein group contained myosin, tubulin polymerization promoting protein, fatty-acid binding protein, uncharacterized DM9, heat shock protein 90 cochaperone tebp P-23, and antigen S. When the serological applicability of recombinant forms of these proteins was assessed using enzyme-linked immunosorbent assay, DM9 protein (rEgDM9) showed 90.1% sensitivity (73/81 sera tested) and 94.5% specificity (172/181 sera tested), respectively. rEgDM9 showed weak cross-reactions with patient sera from the transitional and chronic stages of cystic echinococcosis (3 to 5 stages). rEgDM9 would serve as a useful alternative antigen for serodiagnosis of both early- and advanced-stage AE cases.

• Geomechanics and Engineering
• /
• 제29권4호
• /
• pp.377-390
• /
• 2022

The stability of the roof rock-coal pillar-floor rock composite structure is of great significance to coal mine safety production. The cracks existing in the composite structure seriously affect the stability of the roof rock-coal pillar-floor rock composite structure. The numerical simulation tests of rock-coal-rock composite structures with different crack characteristics were carried out to reveal the composite structures' mechanical properties and failure mechanisms. The test results show that the rock-coal-rock composite structure's peak stress and elastic modulus are directly proportional to the crack angle and inversely proportional to the crack length. The smaller the crack angle, the more branch cracks produced near the main control crack in the rock-coal-rock composite structure, and the larger the angle between the main control crack and the crack. The smaller the crack length, the larger the width of the crack zone. The impact energy index of the rock-coal-rock composite structure decreases first and then increases with the increase of crack length and increases with the increase of crack angle. The functional relationships between the different crack characteristics, peak stress, and impact energy index are determined based on the sensitivity analysis. The determination of the functional relationship can fully grasp the influence of the crack angle and the crack length on the peak stress and impact energy index of the coal-rock composite structure. The research results can provide a theoretical basis and guidance for preventing the instability and failure of the coal pillar-roof composite structure.

• 감성과학
• /
• 제21권3호
• /
• pp.115-128
• /
• 2018

티베트 고원에서 살고 있는 티베트인 또는 장족은 오랜 역사와 자연환경에 순응한 자신만의 독특한 생활양식을 가지고 있어 독자적인 의복문화 형태를 지닌다. 복식 중에 색채, 문양, 도안의 사용에서 종교적인 의미를 나타낼 뿐 아니라, 복식을 통해 자신의 생활의 희망과 자연을 존중한 마음을 강하게 표현하고 있다. 티베트 민족 복식의 색채 상징성에 관한 고찰을 통하여 티베트족 문화의 특수성을 이해하고자 장족 복식에 사용된 색채를 분석하고 그들만의 색채의 의미를 고찰하였다. 장족의 색채인식, 상징성을 색의 사용 예시와 출현빈도를 중심으로 살펴보았다. 연구방법으로는 복식사진의 색채 수집 및 분석을 실시하였는데 I.R.I HUE-TONE 시스템에서 매칭 색상과 색조를 찾는 방식이었다. 장족 복식도서의 칼라사진과 청해 장족문화박물관의 장족 복식을 직접 촬영한 사진, 인터넷 박물관의 복식사진 등 총 96장의 사진이었다. 결과는 첫째, 세상을 구성한다고 생각하는 가장 주요한 요소를 5가지 색 장오색에 연결하여 각 색마다 자연적 의미를 부여하는데, 빨간색은 불, 파란색은 하늘, 흰색은 구름과 흰 눈, 초록색은 초원, 노란색은 대지를 각기 상징한다. 둘째, 홍과 녹, 흑과 백, 홍과 남, 황과 자색 등 강렬한 색채조화를 이루도록 대담한 색채를 사용하고 있는 것이 특징이다. 셋째, 티베트인들의 특유한 심미관을 표현하는데 색의 일반적인 감성을 대부분 따르지만 그들만의 감성이 들어있는 색상이 존재하였다.