• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.8
• /
• pp.1183-1189
• /
• 2017

Objective: Owing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and the identification of gene regulatory networks through various informatics tools. However, to evaluate the biofunctional activity of a predicted target gene, in vivo and in vitro functional genomic analyses should be a prerequisite. Methods: Due to a lack of quail genomic sequence information, we first identified the partial genomic structure and sequences of the quail SH3 domain containing ring finger 2 (SH3RF2) gene. Subsequently, SH3RF2 was knocked out using clustered regularly interspaced short palindromic repeat/Cas9 technology and single cell-derived SH3RF2 mutant sublines were established to study the biofunctional activity of SH3RF2 in quail myoblast (QM7) cells during muscle differentiation. Results: Through a T7 endonuclease I assay and genotyping analysis, we established an SH3RF2 knockout (KO) QM7#4 subline with 61 and 155 nucleotide deletion mutations in SH3RF2. After the induction of myotube differentiation, the expression profiles were analyzed and compared between regular QM7 and SH3RF2 KO QM7#4 cells by global RNA sequencing and bioinformatics analysis. Conclusion: We did not detect any statistically significant role of SH3RF2 during myotube differentiation in QM7 myoblast cells. However, additional experiments are necessary to examine the biofunctional activity of SH3RF2 in cell proliferation and muscle growth.

• Journal of Korean Society for Quality Management
• /
• v.45 no.2
• /
• pp.247-260
• /
• 2017

Purpose: The study is aimed to identify the national cultural dimensions that are affecting the quality management (QM) maturity level and the project quality performance, and analyze their relationships. Methods: This study collected the data of QM Maturity level based on Crosby's QM maturity model and the project quality performance using the Iron Triangle (Quality, Time/Schedule and Cost) from the employees who are participating in the ITER Project across the major 8 countries (China, France, Italy, Japan, Korea, Russia, U.K. and U.S.A.). Three research hypotheses are proposed concerning the national cultural dimensions in this study and Hofstede's five cultural dimensions framework are used for the statistical test. Results: The results are two folds in the study: First, there is a significant positive correlation between the QM maturity level and the project quality performance. Second, three cultural dimensions (Collectivism, Large Power Distance and Strong Uncertainty Avoidance) and five cultural dimensions (Collectivism, Large Power Distance, Strong Uncertainty Avoidance, Feminity and Long Term Orientation) have a positive impact on the QM maturity level and the project quality performance respectively. Conclusion: From the results, the understanding and consideration of the culture difference among the countries participating International Collaboration R&D project are recommended.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.8
• /
• pp.1371-1379
• /
• 2015

The Ca_v1.2 Ca²⁺ channel is essential for cardiac and smooth muscle contractility and many physiological functions. We mutated single, double, and quadruple sites of the four potential Asn (N)-glycosylation sites in the rabbit Ca_v1.2 into Gln (Q) to explore the effects of Nglycosylation. When a single mutant (N124Q, N299Q, N1359Q, or N1410Q) or Ca_v1.2/WT was expressed in Xenopus oocytes, the biophysical properties of single mutants were not significantly different from Ca_v1.2/WT. In comparison, the double mutant N124,299Q showed a positive shift in voltage-dependent gating. Furthermore, the quadruple mutant (QM; N124,299,1359,1410Q) showed a positive shift in voltage-dependent gating as well as a reduction of current. We tagged EGFP to the QM, double mutants, and Ca_v1.2/WT to chase the mechanisms underlying the reduced currents of QM. The surface fluorescence intensity of QM was weaker than that of Ca_v1.2/WT, suggesting that the reduced current of QM arises from its lower surface expression than Ca_v1.2/WT. Tunicamycin treatment of oocytes expressing Ca_v1.2/WT mimicked the effects of the quadruple mutations. These findings suggest that Nglycosylation contributes to the surface expression and voltage-dependent gating of Ca_v1.2.

• Bulletin of the Korean Space Science Society
• /
• 2003.10a
• /
• pp.51-51
• /
• 2003

인공위성의 개발 단계는 Prototype Model(PM), Engineering Model(EM), Qualification Model(QM) 그리고 Flight Model(FM)로 구분된다. 이때, Prototype 개발을 제외한 EM, QM, FM 개발단계는 반드시 종합조립시험(AIT)을 통하여 System Integration Test를 수행한다. 이 중에서 위성발사 전 최종 종합조립시험단계인 FM AIT는 위성의 최종시험단계이므로 Scenario Test를 포함한 지상에서 수행하여야 할 모든 시험을 수행한다. 이때, EM, QM 단계와는 다르게 FM AIT는 전기적 및 기계적 시험을 수행하나, 본 논문에서는 전기적 시험 과정과, Control Center 구성도 등을 포함하고 있는 Hardware 부분과, 관련 시험을 수행하게 될 Simulator를 포함한 Software 부분으로 나누어 소개하며, 기타 FM AIT 수행에 필요한 Electrical Ground Support System (EGSE) 전체 구성을 소개한다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 1997.04a
• /
• pp.326-329
• /
• 1997

The surface of mica/epoxy paper(130${\mu}{\textrm}{m}$) is investigated with SEM(scanning electron microscope). SEM micrographs revealed that thermal aging has a strong influence on aged specimens. The mica/epoxy paper was exposed at 187$^{\circ}C$ in an ordinary electrical oven for 72hour. Stater windings from two generators were measured by tan delta(tan $\delta$)and maximum partial discharge(Qm). The values of tan $\delta$and Qm were fecund to be higher for new generator than old one. The insulation condition of mica/epoxy paper were measured by ac current and tan $\delta$. Test results are observed that the sound specimens are higher than those aged. Model bar coils subjected to various accelerated agings were evaluated by Qm.

• Journal of Korean Society of Industrial and Systems Engineering
• /
• v.25 no.4
• /
• pp.32-39
• /
• 2002

This study investigated the management environment of medium and small business by examining the role of medium and small business in national economics, the change in management condition, and the problems in quality and technique. Also we analyzed problems in the past quality control activity of our medium and small business, and examined the driving direction and introduction method of QM. And to understand the effect of manager's concern in quality management to observe the quality system before and after introduction of QM. A target for this investigation is the medium and small business which has introduced QM through ISO 9000.

• Korean Journal of Microbiology
• /
• v.16 no.4
• /
• pp.141-147
• /
• 1978

In order to increase the productivity of cellulolytic enzymes, medium composition and culture conditions were studied. When cellulose powder (Avicel) supplemented with rice straw was used as carbon source, productivity of ${\beta}-glucosidase$ was increased by about 3 times compared with the runs with only cellulose powder as a carbon source. In this case no negative effects on the production of CMC enzyme activity and filter paper activity was found. For the production of celulolytic enzymes using T. reesei QM 9414, casitone was found to be a good nitrogen source compared with other sources studied, such as peptone, yeast extract, tryptone, and casein. The highest cellulase activity was attained when 0.3% glucose and 0.01% Tween 80 were supplemented to the standard medium of Rese. An adequate oxygen transfer rate was also found to be important to the cellulase fermentation and about 50 mmole of oxygen/liter/hour supported good cellulase biosynthesis during cellulase fermentation.

• Proceedings of the Korea Society for Industrial Systems Conference
• /
• 1997.11a
• /
• pp.475-488
• /
• 1997

This study investigated the management environment of medium and small business by examining the role of medium and small business in national economics, the change in management condition, and the problems in quality and technique. Also we analyzed problems in the past quality control activity of our medium and small business, and examined the driving direction and intorduction method of QM. And to understand the effect of manager's concern in quality management to observe the quality system before and after introduction of QM. A target for this investigation is the medium and small business which has introduced QM through ISO 9000. Also analyzed how 05 observance effects to the sale price, process effect customer complaint and on-time delibery by an oral test.

• Cement
• /
• s.129
• /
• pp.41-45
• /
• 1992

• Korean Journal of Poultry Science
• /
• v.47 no.3
• /
• pp.127-133
• /
• 2020

Chibby family member 2 (CBY2), also known as SPERT or NURIT, is a gene with Chibby-like super family domain, whose function is not well known. In this study, the quail CBY2 gene was cloned, its sequences were analyzed, and its role in the myogenesis of QM7 quail muscle cells was characterized. Quail CBY2 has 978 nucleotides, which are translated into 325 amino acids, and the amino acid sequences are highly similar to those of chicken CBY2. Avian CBY2 diverted from mammalian CBY2 during early evolutionary history. According to the protein domain prediction analysis, quail CBY2 has a Chibby-like superfamily domain consisting of 83 amino acids at the N-terminal of the protein, although compared to mammalian CBY2, many of the amino acids were different. CBY2 was highly expressed in the adipose tissue and moderately expressed in the liver, heart, and kidney, whereas rarely expressed in the muscle tissue in quail. To characterize the role of CBY2 in myogenesis, CBY2 was overexpressed in QM7 cells. The overexpression of CBY2 inhibited myotube formation as shown that the myotube area was approximately only 25% that of the control. Taken together, quail CBY2 has a Chibby-like superfamily domain and inhibits myogenesis. Further studies should focus on the identification of the inhibitory mechanism of CBY2 on myogenesis.