• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.5
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• pp.725-732
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• 2020

The sudden drop of water temperature in winter is very threatening factor that affects the productivity of farmed fish and the management in aquafarm. In this study, we investigated the effect of low temperature on the survival, oxygen consumption and stress responses of parrotfish Oplegnathus fasciatus due to acute drop of water temperature. The survival rate of parrotfish Oplegnathus fasciatus was 5% at 6℃, 95% at 8℃ and 100% at 10℃ on the 4^th day of exposure in each experimental temperature. Low-lethal temperature for 4days of parrotfish Oplegnathus fasciatus (4 day-LT₅₀) was 6.99℃ (confidence limit, 6.55-7.42℃). Oxygen consumption rate was significantly decreased with decreasing water temperature. Temperature coefficient (Q₁₀) was found to be 4.0 between 10℃ and 8℃ and 0.39 between 8℃ and 6℃. As a result of investigating the stress response according to the drop in water temperature, the concentration of SOD (Superoxide dismutase), cortisol, glucose, total Ig, AST (Aspartate) and ALT (Alanine aminotransferase) increased with decreasing of water temperature. This study would be useful for the management of temperature about cultured fish.

• Journal of the Korean Society for Heat Treatment
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• v.10 no.3
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• pp.198-208
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• 1997

The-steady-state creep mechanism and behavior of Zircaloy-4 used as cladding materials in PWR have been investigated in air environment over the temp, ranges from 600 to $645^{\circ}C$ and stress ranges from 4 to $7kg/mm^2$. The stress exponents for the creep deformation of this alloy, n were decreased 4.81, 4.71, 4.64, and 4.56 at 600, 615, 630 and $645^{\circ}C$, respectively; the stress exponents decreased with increasing the temperature and got closer to about 5. The apparent activation energies, Q, were 62.1, 60.0, 57.9 and 55.4 kcal/mole at stresses of 4, 5, 6, $7kg/mm^2$, respectively; the activation energies decreased with increasing the stress and were close to those of volume self diffusion of Zr in Zr-Sn-Fe-Cr system. In results, it can be considered that the creep deformation for Zircaloy-4 was controlled by the dislocation climb over the ranges of this experimental conditions. Larson-Miller parameter, P, for the crept specimens was obtained as P=(T+460)(logt,+23). The failure plane observed by SEM slightly showed up intergranular fracture at this experiment ranges. However, it was essentially dominated by the dimple phenomenon, which was a characteristics of the transgranular fracture.

• 한국정보디스플레이학회:학술대회논문집
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• 2007.08a
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• pp.448-451
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• 2007

DuPont Teijin $Films^{TM}$ (DTF) have developed engineered substrates specifically for the flexible electronics market. $Teonex^{(R)}$ Q65, $Melinex^{(R)}$ ST506 and ST504 are biaxially oriented crystalline polyesters with the option of planarised surfaces. These films are emerging as competitive materials for the base substrate in OLED displays and active matrix backplanes. Given the demanding dimensional reproducibility requirements in the display applications, it is critical to control the several factors that can influence the film distortion in order to achieve the ultimate performance. This paper will discuss the impact of thermal stress, mechanical stress and the processing environment on dimensional reproducibility of polyester film and give examples of how this impacts on the film in device manufacture.

• Journal of the Korean Geotechnical Society
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• v.19 no.4
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• pp.83-93
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• 2003

This research, in order to study the effects of initial shear stress of anisotropically consolidated sand that has 0.558% fines, performed several undrained static and dynamic triaxial test. To simulate the real field conditions, loose and dense samples were prepared. Besides, the cyclic shear strength of Nakdong River sand under various combinations of initial static shear stress, stress path, pore water pressure and residual strength relationship was studied. By using Bolton's theory, peak internal friction angle at failure which has considerable effects on the relative density and mean effective stress was determined. In p'- q diagram, the phase transformation line moves closer to the failure line as the specimen's initial anistropical consolidation stress increases. Loose sands were more affected than dense sands. The increase of consolidation stress ratio from 1.4 to 1.8 had an effect on liquefaction resistance strength resulting from the increase of relativity density, and showed similar CSR values in dense specimen condition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.379-388
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• 2016

There are various system defects from weapons manufacturing due to the numerous production processes and various production environments. The first kind of defect is patent defects, which can be detected by visual inspection, functional testing, and existing quality control procedures during the manufacturing process. The second kind is latent defects, which cannot be detected though existing quality management approaches because of the complexity of the system and manufacturing process. To minimize the initial defect problems, environmental stress screening (ESS) is needed to detect the defects, remove them, and improve the product conditions based on the environmental stress conditions of temperature and vibration. We implemented a tool for quantitative ESS effectiveness analysis and profile design automation based on MIL-HDBK-344 and verified it using six scenarios with different temperature stress, vibration stress, and test designs.

• Development and Reproduction
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• v.22 no.1
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• pp.73-83
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• 2018

This study investigates the endoplasmic reticulum (ER) stress and subsequent apoptosis in duced during somatic cell nuclear transfer (SCNT) process of porcine SCNT embryos. Porcine SCNT and in vitro fertilization (IVF) embryos were sampled at 3 h and 20 h after SCNT or IVF and at the blastocyst stage for mRNA extraction. The x-box binding protein 1 (Xbp1) mRNA and the expressions of ER stress-associated genes were confirmed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. Before commencing SCNT, somatic cells treated with tunicamycin (TM), an ER stress inducer, confirmed the splicing of Xbp1 mRNA and increased expressions of ER stress-associated genes. In all the embryonic stages, the SCNT embryos, when compared with the IVF embryos, showed slightly increased expression of spliced Xbp1 (Xbp1s) mRNA and significantly increased expression of ER stress-associated genes (p<0.05). In all stages, apoptotic gene expression was slightly higher in the SCNT embryos, but not significantly different from that of the IVF embryos except for the Bax/Bcl2L1 ratio in the 1-cell stage (p<0.05). The result of this study indicates that excessive ER stress can be induced by the SCNT process, which induce apoptosis of SCNT embryos.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.21.1-21.11
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• 2016

Background: The evaluation of suitable reference genes as normalization controls is a prerequisite requirement for launching quantitative reverse transcription-PCR (RT-qPCR)-based expression study. In order to select the stable reference genes in abalone Haliotis discus hannai tissues (gill and hepatopancreas) under heavy metal exposure conditions (Cu, Zn, and Cd), 12 potential candidate housekeeping genes were subjected to expression stability based on the comprehensive ranking while integrating four different statistical algorithms (geNorm, NormFinder, BestKeeper, and ${\Delta}CT$ method). Results: Expression stability in the gill subset was determined as RPL7 > RPL8 > ACTB > RPL3 > PPIB > RPL7A > EF1A > RPL4 > GAPDH > RPL5 > UBE2 > B-TU. On the other hand, the ranking in the subset for hepatopancreas was RPL7 > RPL3 > RPL8 > ACTB > RPL4 > EF1A > RPL5 > RPL7A > B-TU > UBE2 > PPIB > GAPDH. The pairwise variation assessed by the geNorm program indicates that two reference genes could be sufficient for accurate normalization in both gill and hepatopancreas subsets. Overall, both gill and hepatopancreas subsets recommended ribosomal protein genes (particularly RPL7) as stable references, whereas traditional housekeepers such as ${\beta}-tubulin$ (B-TU) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were ranked as unstable genes. The validation of reference gene selection was confirmed with the quantitative assay of MT transcripts. Conclusions: The present analysis showed the importance of validating reference genes with multiple algorithmic approaches to select genes that are truly stable. Our results indicate that expression stability of a given reference gene could not always have consensus across tissue types. The data from this study could be a good guide for the future design of RT-qPCR studies with respect to metal regulation/detoxification and other related physiologies in this abalone species.

• Journal of the Korean Society for Advanced Composite Structures
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• v.4 no.1
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• pp.1-8
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• 2013

The finite element analysis (FEA) is a numerical technique to find solutions of field problems. A field problem is approximated by differential equations or integral expressions. In a finite element, the field quantity is allowed to have a simple spatial variation in terms of linear or polynomial functions. This paper represents a review and an accuracy-study of the finite element method comparing the FEA results with the exact solution. The exact solutions were calculated by solid mechanics and FEA using matrix stiffness method. For this study, simple bar and cantilever models were considered to evaluate four types of basic elements - constant strain triangle (CST), linear strain triangle (LST), bi-linear-rectangle(Q4),and quadratic-rectangle(Q8). The bar model was subjected to uniaxial loading whereas in case of the cantilever model moment loading was used. In the uniaxial loading case, all basic element results of the displacement and stress in x-direction agreed well with the exact solutions. In the moment loading case, the displacement in y-direction using LST and Q8 elements were acceptable compared to the exact solution, but CST and Q4 elements had to be improved by the mesh refinement.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.8 no.1
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• pp.28-36
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• 1996

The influence of vertical eddy viscosity to the nonlinear interaction of tidal current and wind-induced current is examined using a point model. A zero-equation turbulence model is derived by simplifying the q$^2$-q$^2$1 turbulence model under the assumption that the generation of turbulence kinetic energy is balanced with its dissipation and is further modified to include the depth of frictional influence properly The zero-equation turbulence model is derived and the possibility of resonance in the presence of Coriolis effect is suggested. The amplitudes of tidal currents remain the same regardless of the applied wind stress, but the over-tide component is generated due to the nonlinear interaction of tidal current and wind-induced current. Significant changes in the vertical profile of wind-induced currents can occur according to tide-induced background turbulence. The turbulence model can give rise to misleading results when applied to the wind-driven circulation in the tide-dominated sea such as Yellow Sea unless the tide-induced background turbulence is adequately included in the parameterization of vertical eddy viscosity.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.247-253
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• 2002

The cellular responses of TNT-degrading bacterium, Stenotrophomonas sp. OK-5 to explosive 2,4,6-trini-trotoluene (TNT) as an environmental contaminant were examined. Survival of the strain OK-5 with time in the presence of different concentrations of TNT under sublethal conditions was monitored, and viable counts paralleled the production of the stress shock proteins in this bacterium. Total cellular fatty acids analysis showed that strain OK-5 produced or disappeared several different kinds of lipids when grown on TNT media than when grown on TSA. Under scanning electron microscope, the cells treated with 0.5 mM TNT for 12 hrs showed irregular rod shapes with wrinkled surfaces. Analyses of SDS-PAGE and Western blot using anti-DnaK and anti-GroEL revealed that several stress shock proteins including 70 kDa DnaK and 60 kDa GroEL in strain OK-5 were newly synthesized at different TNT concentrations in exponentially growing cultures. 2-D PAGE of soluble protein fractions from the culture of OK-5 exposed to TNT demonstrated that approximately 300 spots were observed on the silver stained gel ranging from pH 3 to pH 10. Among them, 10 spots significantly induced and expressed in response to TNT were selected and analyzed. As the result of internal amino acid sequencing with ESI-Q TOF, two proteins, spot #1 and spot #10 were assigned the DnaK protein XF2340 of Xylella fastidiosa and stress-induced protein of Mesorhizobium loti, respectively.