• Journal of Ginseng Research
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• v.42 no.2
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• pp.149-157
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• 2018

Background: Panax notoginseng leaves (PNL) exhibit extensive activities, but few analytical methods have been established to exclusively determine the dammarane triterpene saponins in PNL. Methods: Ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF MS) and HPLC-UV methods were developed for the qualitative and quantitative analysis of ginsenosides in PNL, respectively. Results: Extraction conditions, including solvents and extraction methods, were optimized, which showed that ginsenosides Rc and Rb3, the main components of PNL, are transformed to notoginsenosides Fe and Fd, respectively, in the presence of water, by removing a glucose residue from position C-3 via possible enzymatic hydrolysis. A total of 57 saponins were identified in the methanolic extract of PNL by UPLC/Q-TOF MS. Among them, 19 components were unambiguously characterized by their reference substances. Additionally, seven saponins of PNL-ginsenosides Rb1, Rc, Rb2, and Rb3, and notoginsenosides Fc, Fe, and Fd-were quantified using the HPLC-UV method after extraction with methanol. The separation of analytes, particularly the separation of notoginsenoside Fc and ginsenoside Rc, was achieved on a Zorbax ODS C8 column at a temperature of $35^{\circ}C$. This developed HPLC-UV method provides an adequate linearity ($r^2$ > 0.999), repeatability (relative standard deviation, RSD < 2.98%), and inter- and intraday variations (RSD < 4.40%) with recovery (98.7-106.1%) of seven saponins concerned. This validated method was also conducted to determine seven components in 10 batches of PNL. Conclusion: These findings are beneficial to the quality control of PNL and its relevant products.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11b
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• pp.188-195
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• 2000

This study was conducted to measure the moisture content of powdered food using RF impedance spectroscopic method. In frequency range of 1.0 to 30㎒, the impedance such as reactance and resistance of parallel plate type sample holder filled with wheat flour and red-pepper powder of which moisture content range were 5.93∼-17.07%w.b. and 10.87 ∼ 27.36%w.b., respectively, was characterized using by Q-meter (HP4342). The reactance was a better parameter than the resistance in estimating the moisture density defined as product of moisture content and bulk density which was used to eliminate the effect of bulk density on RF spectral data in this study. Multivariate data analyses such as principal component regression, partial least square regression and multiple linear regression were performed to develop one calibration model having moisture density and reactance spectral data as parameters for determination of moisture content of both wheat flour and red-pepper powder. The best regression model was one by the multiple linear regression model. Its performance for unknown data of powdered food was showed that the bias, standard error of prediction and determination coefficient are 0.179% moisture content, 1.679% moisture content and 0.8849, respectively.

• Nuclear Engineering and Technology
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• v.55 no.4
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• pp.1225-1232
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• 2023

The ¹⁸⁰W is the lightest isotope of Tungsten with small abundance ratio. It is slightly radioactive (α decay), with an extremely long half-life. Its separation is possible by non-conventional single withdrawal cascades. The ¹⁸⁰W is used in radioisotopes production and study of metals through gamma-ray spectroscopy. In this paper, single withdrawal cascade model is developed to evaluate multicomponent separation in non-conventional transient cascades, and available experimental results are used for validation. Numerical studies for separation of ¹⁸⁰W in a transient single withdrawal cascade are performed. Parameters affecting the separation and equilibrium time of cascade such as number of stages, cascade arrangements, feed location and flow rate for a fixed number of gas centrifuges (GC) are investigated. The Salp Swarm Algorithm (SSA) as a bio-inspired optimization algorithm is applied as a novel method to minimize the feed consumption to obtain desired concentration in the collection tank. Examining different cascade arrangements, it is observed in arrangements with more stages, the separation is further efficient. Based on the obtained results, with increasing feed flow rate, for fixed product concentration, the cascade equilibrium time decreases. Also, it is shown while the feed location is the farthest stage from the collection tank, the separation and cascade equilibrium time are well-organized. Finally, using SSA optimal parameters of the cascade is calculated, and optimal arrangement to produce 5 gr of ¹⁸⁰W with 90% concentration in the tank, is proposed.

• Journal of Embryo Transfer
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• v.24 no.3
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• pp.225-230
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• 2009

Sex-sorting of sperm is an assisted reproductive technology (ART) used by the livestock industry for the mass production of animals of a desired sex. The standard method for sorting sperm is the detection of DNA content differences between X and Y chromosome-bearing sperm by flow cytometry. However, this method has variable efficiency and therefore requires verification by a second method. We have developed a sex determination method based on quantitative real-time polymerase chain reaction (qPCR) of the porcine amelogenin (AMEL) gene. The AMEL gene is present on both the X and the Y chromosome, but the length and sequence of its noncoding regions differ between the X and Y chromosomes. By measuring the threshold cycle (Ct) of qPCR, we were able to calculate the relative frequency of X chromosome. Two sets of AMEL primers were used in these studies. One set (AME) targeted AMEL gene sequences present in both X and Y chromosome, but produced PCR products of different lengths for each chromosome. The other set (AXR) bound to AMEL gene sequences present on the X chromosome but absent esholthe Y-chromosome. Relative product levels were calculated by normalizing the AXR fluorescence to the AME fluorescence. The AMEL method accurately predicted the sex ratios of boar sperm, demonstrating that it has potential value as a sex determination method.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.30-37
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• 2011

This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 ${\mu}l$ of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.40 no.3
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• pp.18-26
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• 2017

Defense industries which require high reliability need an optimized quality management system with well-planned implementation. And the government should examine the overall status of defense industries, then establish practical policies with a proper support plan in required areas to upgrade the quality management level of manufacturers. Thus, DTaQ developed the model for 2 years from 2014, which specialized in quality management level analysis for defense industries. And a survey has been undertaken with that model by DTaQ and Korea Research Center in 2016. The surveyed companies randomly sampled among those which have more than 30 employees and delivery history over past 3 years, and finally 106 defense industries were selected. This paper present survey method and indexes for survey of defense industry quality management level. The survey was conducted in the order of planning, data collection and data processing, and the validity and reliability of the data were verified to increase objectivity of survey results. The survey contents mainly consist of system quality and management quality. System quality includes Product Development Management, Production Operation Management, supply chain quality management, Safety & Environment Management and Reliability Management, on the other hand, management quality includes Strategic Leadership, Human Resource Management, Customer Market Management and Information & Knowledge Management. Thus this proposes the current overall quality management status of the 106 defense industries and shows level differences by company sizes and manufacturing sectors based on the result of survey. Specifically, this paper enables to track the areas which need prompt government support with the policy directions to make quality management level higher. Therefore, it is expected that this can be used as reference data in establishing quality policies for military supplies in the future.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.144-151
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• 2014

Increasing the gene copy number has been commonly used to enhance the protein expression level in the yeast Pichia pastoris. However, this method has been shown to be effective up to a certain gene copy number, and a further increase of gene dosage can result in a decrease of expression level. Evidences indicate the gene dosage effect is product-dependent, which needs to be determined when expressing a new protein. Here, we describe a direct detection of the gene dosage effect on protein secretion through expressing the enhanced green fluorescent protein (EGFP) gene under the direction of the ${\alpha}$-factor preprosequence in a panel of yeast clones carrying increasing copies of the EGFP gene (from one to six copies). Directly examined under fluorescence microscopy, we found relatively lower levels of EGFP were secreted into the culture medium at one copy and two copies, substantial improvement of secretion appeared at three copies, plateau happened at four and five copies, and an apparent decrease of secretion happened at six copies. The secretion of EGFP being limiting at four and five copies was due to abundant intracellular accumulation of proteins, observed from the fluorescence image of yeast and confirmed by western blotting, which significantly activated the unfolded protein response indicated by the up-regulation of the BiP (the KAR2 gene product) and the protein disulfide isomerase. This study implies that tagging a reporter like GFP to a specific protein would facilitate a direct and rapid determination of the optimal gene copy number for high-yield expression.

• Journal of the Korea Society of Computer and Information
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• v.11 no.3
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• pp.141-149
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• 2006

In this paper, we introduce an improved algorithm for computing matrix triple product that commonly arises in primal-dual optimization method. In computing $P=AHA^{t}$, we devise a single pass algorithm that exploits the block diagonal structure of the matrix H. This one-phase scheme requires fewer floating point operations and roughly half the memory of the generic two-phase algorithm, where the product is computed in two steps, computing first $Q=HA^{t}$ and then P=AQ. The one-phase scheme achieved speed-up of 2.04 on Intel Itanium II platform over the two-phase scheme. Based on memory latency and modeled cache miss rates, the performance improvement was evaluated through performance modeling. Our research has impact on performance tuning study of complex sparse matrix operations, while most of the previous work focused on performance tuning of basic operations.

• Proceedings of the KIEE Conference
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• 1996.07a
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• pp.262-264
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• 1996

This paper presents a new dual loop control using novel vector phase locked loop(VP-PLL) for a high power static var compensator(SVC) with three-level GTO voltage source inverter(VSI). Through circuit DQ-transformation, a simple dq-axis equivalent circuit is obtained. From this, DC analysis is carried out to obtain maximum controllable phase angle ${\alpha}_{max}$ per unit current between the three phase source and the switching function of inverter, and AC open-loop transfer function is given. Because ${\alpha}_{max}$ becomes small in high power SVC, this paper proposes VP-PLL for more accurate $\alpha$-control. As a result, the overall control loop has dual loop structure, which consists of inner VP-PLL for synchronizing the phase angle with source and outer Q-loop for compensating reactive power of load. Finally, the validity of the proposed control method is verified through the experimental results.

• Journal of Korean Society for Quality Management
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• v.41 no.2
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• pp.197-208
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• 2013

Purpose: This study proposed a systematic approach to measure eco-efficiency of products based on the concept of quality function deployment(QFD). QFD method has been used to convert customer's subjective requirements to design specifications. A extension of QFD to environmental issues is known as quality environmental function deployment(QEFD). QEFD includes environmental requirements into customer's demand for quality, and incorporates them to design specifications of a product. Methods: In this study, we compared eco-efficiency of old and new models through application of QEFD. Results: The result revealed that the new model's eco-efficiency improved 24% better than the old model. Also, we found that user's Factor X of the new model has been improved to 1.178, which is about 18% better than that of the old model. Conclusion: This study implied that we can systematically use QEFD to determine eco-efficiency of two comparable products in any industries.