Browse > Article

Early Diagnostic Method of Avian Influenza Virus Subtype Using Ultra Real-Time PCR  

Kim, Sang-Tae (Divion of Biological and Phamaceutical, Ewha Womans University)
Kim, Young-Kyoon (Department of Forest Products, College of Forest Science, Kookmin University)
Kim, Jang-Su (Department of Laboratory Medicine, Korea University Guro Hospital)
Publication Information
Korean Journal of Microbiology / v.47, no.1, 2011 , pp. 30-37 More about this Journal
Abstract
This ultra real-time PCR (UPCR) based diagnosis system for avian influenza A virus (AIV) subtype was designed. The target primer in this study was derived from H5N1 subtype-specific 133 bp partial gene of hemagglutinin (HA), and was synthesized by using PCR-based gene synthesis on the ground of safety. UPCR was operated by Mini-Opticon Q-PCR Quantitative Thermal Cycler using aptamer-based molecular beacon, total 10 ${\mu}l$ of reaction mixture with extraordinarily short time in each steps in PCR. The detection including UPCR and analysis of melting temperature was totally operated within 15 min. The AIV-specific 133 bp PCR product was correctly amplified until 5 molecules of HA gene as minimum of templates. This kind of PCR was drafted as UPCR in this study and it could be used to detect not only AIV subtype, but also other pathogens using UPCR-based diagnosis.
Keywords
avian influenza virus; hemagglutinin; molecular beacon; ReadyCell;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Alexander, D.J. 1995. The epidemiology and control of avian influenza and Newcastle disease. J. Comp. Pathol. 112, 105-126.   DOI   ScienceOn
2 Basuno, E., Y. Yusdja, and N. Ilham. 2010. Socio-economic impacts of avian influenza outbreaks on small-scale producers in Indonesia. Trans Emerg. Dis. 57, 7-10.   DOI   ScienceOn
3 Ben, S.M., R. Meir, R. Haddas, E. Lapin, I. Shkoda, I. Raibstein, S. Perk, and I. Davidson. 2010. Development of a real-time TaqMan RT-PCR assay for the detection of H9N2 avian influenza viruses. J. Virol. Methods 168, 72-77.   DOI
4 Boehm, K.M. 2010. Seasonal and avian influenza knowledge base of attending physicians in a community-based hospital: a survey-based study. J. Am. Osteopath. Assoc. 110, 285-289.
5 Bosch, F.X., M. Orlich, H.D. Klenk, and R. Rott. 1979. The structure of the hemagglutinin. A determinant for the pathogenicity of influenza viruses. Virology 95, 197-207.   DOI   ScienceOn
6 Butz, K., C. Denk, B. Fitscher, I. Crnkovic-Mertens, A. Ullmann, C.H. Schroder, and F. Hoppe-Seyler. 2001. Peptide aptamers targeting the hepatitis B virus core protein: a new class of molecules with antiviral activity. Oncogene 45, 6579-6586.
7 Castrucci, M.R. and Y. Kawaoka. 1993. Biologic importance of neuraminidase stalk length in influenza A virus. J. Virol. 67, 759-764.
8 Claas, E.C., J.A.D. Osterhaus, R. Van Beck, J.C. de Jong, G.F. Rimmelzwaan, D.A. Senne, S. Krauss, K.F. Shortridge, and R.G. Webster. 1998. Human influenza A (H5N1) virus related to highly pathogenic avian influenza virus. Lancet 351, 472-477.   DOI   ScienceOn
9 Criado-Fornelio, A. 2007. A review of nucleic-acid-based diagnostic tests for Babesia and Theileria, with emphasis on bovine piroplasms. Parassitologia 49, 39-44.
10 Deyde, V.M., R. Sampath, R.J. Garten, P.J. Blair, C.A. Myers, C. Massire, H. Matthews, P. Svoboda, M.S. Reed, J. Pohl, and et al. 2010. Genomic signature-based identification of influenza A viruses using RT-PCR/electro-spray ionization mass spectrometry (ESI-MS) technology. PLoS One 12, in press.
11 Edward, C.H., C.V. Johann, K.R. Julia, and P.D. Gog. 2010. Genome packaging in influenza A virus. J. Gen. Virol. 91, 313-328.   DOI   ScienceOn
12 Fader, R.C. 2005. Comparison of the Binax NOW Flu A enzyme immunochromatogra-phic assay and R-Mix shell vial culture for the 2003-2004 influenza season. J. Clin. Microbiol. 43, 6133- 6135.   DOI   ScienceOn
13 Fauci, A.S. 2006. Emerging and re-emerging infectious diseases: Influenza as a prototype of the host-pathogen balancing act. Cell. 124, 665-670.   DOI   ScienceOn
14 Fleming, D.M., P. Chakraverty, C. Sadler, and P. Litton 1995. Combined clinical and virological surveillance of influenza in winters of 1992 and 1993-4. B.M.J. 29, 290-291.
15 Gopinath, S., C. Sakamaki, Y. Kawasaki, and K. Kumar. 2006. An efficient RNA aptamer against human influenza B virus hemagglutinin. J. Biochem. 139, 837-846.   DOI   ScienceOn
16 Guan, M.K., L.C. Hsueh, Y.K. Liang, T.J. Liang, L.C.J. Chulu, H.M. Liao, T.J. Chang, and H.J. Liu. 2006. Development of a quantitative Light Cycler real-time RT-PCR for detection of avian reovirus. J. Virol. Methods 133, 6-13.   DOI   ScienceOn
17 Guan, Y., D. Vijaykrishna, J. Bahl, H. Zhum, J. Wang, and G.J. Smith. 2010. The emergence of pandemic influenza viruses. Protein Cell. 1, 9-13.   DOI   ScienceOn
18 Ito, T., Y. Suzuki, L. Mitnaul, A. Vines, H. Kida, and Y. Kawaoka. 1997. Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species. Virology 227, 493-499.   DOI   ScienceOn
19 Hilleman, M.R. 2003. Critical overview and outlook: pathogenesis, prevention, and treatment of hepatitis and hepatocarcinoma caused by hepatitis B virus. Vaccine 32, 4626-4649.
20 Horimoto, T. and Y. Kawaoka. 2001. Pandemic threat posed by avian influenza A viruses. Clin. Microbiol. Rev. 14, 129-149.   DOI   ScienceOn
21 Jeon, S.H., B. Kayhan, T. Ben-Yedidia, and R. Arnon. 2004. A DNA aptamer prevents influenza infection by blocking the receptor binding region of the viral hemagglutinin. J. Biol. Chem. 279, 48410-48419.   DOI
22 Kim, E.H., D.W. Lee, S.H. Han, S.H. Kwon, and B. Yoon. 2007. Rapid detection method of avian influenza subtype H5N1 using quick real-time PCR. Kor. J. Microbiol. 43, 23-30.
23 Knipe, D.M. and P.M. Howley. 2007. Field Virology, fifth ed., Lippincott Williams & Wilkins Immunol.
24 Kuiken, T.V., M. Pantin-Jackwood, and D.E. Swayne. 2010. Comparative pathology of select agent influenza a virus infections. Vet. Pathol. 47, 893-914.   DOI   ScienceOn
25 Rachel, M., P. Guoyan, Z. Wandy, L. Beatty, K.A. Mihindukulasuriya, A.P.A. Travassos da, R. Vsevolod, L.P. Robert, B. Tesh, H.W. Virgin, and D. Wang. 2009. Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae. J. Virol. 83, 11599-11606.   DOI   ScienceOn
26 Leonardi, G.P. 2010. Rapid identification of 2009 H1N1 influenza A virus using fluorescent antibody methods. Am. J. Clin. Pathol. 134, 910-914.   DOI   ScienceOn
27 Li, Z., C. Ma, Z. Liu, and W. He. 2010. Serologic crossreactivity among humans and birds infected with highly pathogenic avian influenza A subtype H5N1 viruses in China. Immunol. Lett. in press.
28 Rachel, B.S., M.C. Donald, J.C. Corey, G. Elodie, M.R. Ted, and V.B. Panayiotis. 2009. Genome. Biol. 10, R18.   DOI
29 Verhoeff, J.J., L.J. Stalpers, A. Claes, K.E. Hovinga, G.D. Musters, V.W. Peter, D.J. Richel, W.P.J. Leenders, and W.R. van Furth. 2009. Tumour control by whole brain irradiation of anti-VEGF-treated mice bearing intracerebral glioma. Eur. J. Cancer. 45, 3074-3080.   DOI   ScienceOn