• Journal of Plant Biotechnology
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• v.4 no.2
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• pp.45-52
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunes and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and cherry. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.1
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• pp.87-94
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• 2016

Skin damage is mainly caused by environmental factors such as ultraviolet light, heat, and smoking. It is known that reactive oxygen species production is commonly involved in the pathogenesis of skin damage induced by these factors, causing skin aging. Pyrus pyrifolia Nakai continues to be a popular and highly consumed fruit in many countries with known beneficial effects including antitumor, antioxidative, and anti-inflammatory effects. However, there is no evidence of a therapeutic effect of Pyrus pyrifolia extract (PPE) against skin aging via inhibition of mitochondria-mediated apoptosis. In this study, we investigated PPE protective effect against photoaging induced by UVB ($50mJ/cm^2$) in HS68 human dermal fibroblasts. Lactate dehydrogenase assay showed that PPE significantly protected HS68 cells against UVB-induced damage in a dose-dependent manner. Other assays using DCF-DA demonstrated that PPE protected HS68 cells by regulating reactive oxygen species production. PPE also regulated mitochondrial dysfunction and mitochondrial membrane potential induced by UVB, and inhibited UVB-induced caspase-3 activity. These results indicate that PPE protects human dermal fibroblasts from UVB-induced damage by regulating the oxidative defense system.

• Journal of Environmental Science International
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• v.11 no.11
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• pp.1149-1156
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• 2002

The ecological characteristics of Dolbae trees, a valuable genetic resource for native pyrus fruit trees, were investigated at four naturally inhabited areas on Mount Kaya, Gyeongsangnam-Do. Most Dolbae trees were found on the southwest side of an old hiking path to a mountain valley at altitudes of 610∼670 m. The surrounding flora consisted of 46 families, 69 genera, 75 species, and 10 varieties. The natural habitat of the Dolbae trees was basically a deciduous broad-leaved forest with a predominance of Quercus serrata and ligneous plants(xylophytes) from the Fagaceae and Betulaceae families. The habitat was also found to be in a second transition resulting from forest disturbance and exhibited a degree of diversity of 2.901-5.065, based on Shannon-Weiner's index. Examination of the Dolbae trees found in Mount Kaya revealed diameter breast heights(DBHs) ranging from 10 to 60 cm, including six old and large Dolbae trees with a DBH of 50∼60 cm, which will be particularly valuable as a genetic resource. Accordingly, the current results suggest that the study areas are a secondary forest in a typical mid-temperate zone resulting from forest damage about 10 years earlier.

• Journal of Korean Society of Forest Science
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• v.59 no.1
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• pp.37-45
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• 1983

Taxonomic distribution of ecto- and endomycorrhizae among woody species growing in Korea was studied and their distribution was compared with foreign literature, Most of root samples were collected from Central Branch Station of forest Research Institute in Gwangneung, Gyonggido. A total of 32 families, 63 genera, 102 species were examined. Ectomycorrhizae were observed in the following 13 general of Pinaceae (Pinus Larix, Picea, Abies), Salicaceae (Populus, salix), Betulace (Alnus, Betula, Carpinus, Corpylus), Fagaceae(Quercus, Casianea), and Tiliaceae (Tilia), Endomycorrhizae (vesicular-arbuscular) were observed in Populus and the rest of the 49 genera. Rhus was the only genus which did not have either ecto- or endomycorrhizae, while foreign literature listed following genera having both mycorrhizal types: Juniperus, Cupressus, Populus, Salix, Juglans, Alnus, Ulmus, Malus, Pyrus, and Tilia. Juniperus, Ulmus, Pyrus, and Acer which were reported to have facultative ectomycorrhizae were free of ectomycorrhizae, Some morphological characteristics of endomycorrhizae are shown in photogrphs and discussed.

• Korean Journal of Plant Resources
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• v.15 no.2
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• pp.96-106
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• 2002

Five major naturally populated habitats around the Mt. Daeduk, Gangwon province for wild Chuiangne (Pyrus ussuriensis var. acidula) are mostly located on the southeast side of the mountains at 1,216-1,306ｍ in altitude at sea level and are in very good sunney areas. The total of 96 taxa dividing into 40 families, 79 genera 84 species and 12 varieties have in these areas. Most of these plants are heliophytes, which love sunshine, Compositaes such as Artemisia stolonifera, Synurus deltoides, or Rosaceaes like Potentilla fragarioides var. major are also shown. These areas have been unknown destroyed, so it is possibly thought the second transition has been progressed. The index of species diversity of plant groups in these areas is 4.715-5.270 based on the Shannon-Wiener's method. The wild Chuiangne with 34.15％, which are 5-10㎝ DBH, have been found in habitats. The vegetation around the habitats must be managed by the man in order to keep sound growth of the wild Chuiangne.

• Journal of the Korean Wood Science and Technology
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• v.36 no.1
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• pp.1-11
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• 2008

The purpose of this study is to examine the species of wooden members used in the Youngsanjeon Hall of the Sucknamsa temple in Anseong, Korea. Ninety-five wood samples from pillars, head-penetrating ties, beams, corner rafter, rafter, floor board and other wood members were identified. Seven species identified were hard pine, exotic pine, Zelkova serrata, Ginkgo biloba, Diospyros, Quercus(Cerrus) and Pyrus spp. In the case of pillars, eight were hard pines and the others Zelkova and Pyrus. Species of angle rafter were Ginkgo biloba and Zelkova. and those of beams were one hard pine and one Zelkova. Two purlin samples were Quercus(Cerrus) and rafters were hard pines except one Ginkgo and one exotic pine. Roof-filling timbers were identified as Zelkova and Diospyros spp. The other samples of roof-filling timbers were all identified as hard pines. The use of Ginkgo and several hardwoods in addition to hard pines may indicate supply of local logs near the Sucknamsa temple.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04a
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• pp.101-109
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• 2002

A lot of SSRs (simple sequence repeats) in peach and pear from enriched genomic libraries and in peach from a cDNA library were developed. These SSRs were applied to other related species, giving phenograms of 52 Prunus and 60 pear accessions. Apple SSRs could also be successfully used in Pyrus spp. Thirteen morphological traits were characterized on the basis of the linkage map obtained from an $F_2$ population of peach. This map was compiled with those morphological markers and 83 DNA markers, including SSR markers used as anchor loci, to compare different peach maps. Molecular markers tightly linked to new root-knot nematode resistance genes were also found. A linkage map including disease-related genes, pear scab resistance and black spot susceptibility, in the Japanese pear Kinchaku were constructed using 118 RAPD markers. Another linkage map, of the European pear Bartlett, was also constructed with 226 markers, including 49 SSRs from pear, apple, peach and chewy. Maps of other Japanese pear cultivars, i.e., Kousui and Housui, were also constructed. These maps were the first results of pear species.

• Mycobiology
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• v.48 no.3
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• pp.233-239
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• 2020

A small-spored Alternaria was found from black spots of storaged Koerle pear (Pyrus sinkiangensis), one of the economically important fruit in Xinjiang province, China. The morphology is similar to A. limoniasperae but obviously different in secondary conidiophores and conidial septa. A phylogenetic analysis using sequence datasets of ITS, GAPDH, TEF1, RPB2, Alt a1, OPA10-2, and EndoPG genes revealed that it belonged to the Alternaria alternata complex group. Pathogenicity tests illustrated that the fungus was the causal pathogen of black spot on Koerle pear fruit.

• Journal of the Korean Society of International Agriculture
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• v.23 no.5
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• pp.491-496
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• 2011

Breeding for pear resistance to pear psylla (Cacopsylla pyricola) is one of important objective of the National Institute of Horticultural and Herbal Science breeding program. One hundred thirty three accessions from 15 Asian, Chinese and European pear species were investigated for their resistance against pear psylla. The pear psylla resistance was determined based on the following four characteristics: overwintering adult population, the number of eggs and nymphs, and the degree of soot. The different pear species showed varied resistance to pear psylla. Pyrus calleryana and P. betulaefolia indicated the highest antixenosis as ovipositional preference and antibiosis as nymphal feeding and were the most resistant genetic resources. Likewise the European pears (P. communis), 'Conference' and 'Cascade', exhibited little occurrence and damage by pear psylla. These were proved to be promising genetic materials for breeding resistant cultivars because they had good fruit quality and showed resistance to pear paylla. The observed population of overwintering adult, the number of eggs and nymphs of psylla had significant correlation each other.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.134.1-134
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• 2003

Aecial host ranges of four Gymnosporangium species causing cedar-apple rust diseases, G. asiaticum, G. cornutum, 5. japonicum and G. yamadae, were investigated through artificial inoculation. Thirteen species of nine genera among Rosaceous plants, which have been reported as social hosts in Korea, were inoculated with fresh teliospores spores in early days of May of 2000 and of 2001, respectively. In the results, we re-confirmed that there was highly specific relationship between the rust species and aecial hosts and report new aecial hosts of four Gymnosporangium species. Teliospores of G. cornutum collected from Juniperus rigida successively produced spermogonia and aecia only on Sorbus alntifolia, the first report on host alteration of G. cornutum in Korea. Positive responses by teliospores of G. japonicum from J. chinenis of Suwon and from J. chinenis var. horizontalis of Jeju island were obtained only on P. villosa. Crataegus pinnatifida was confirmed as a new aecial host of G. viatium. Until this time, G. ymadae was believed to have Malus as the aecial host. However, teliospores of G. yamadae collected from J. chinensis var. kaizuka successively formed spermogonia and aecia on the leaves of Chaenomeles lagenaria, C. sinensis, Pyrus pyrtifolia var, culta, P. ussuriensis, Malus pumila and M. sileboldii. The date for maturation of spermogonia and aecia, and symptom development varied according to the rust fungi and aecial host plants, respectively.