Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Protective Effects of Pyrus pyrifolia NAKAI Leaf Extracts on UVB-induced Toxicity in Human Dermal Fibroblasts

자외선B 노출로 인해 손상된 피부세포에 대한 돌배나무잎 추출물의 보호효과

  • Koh, Ara (Department of Clinical Pharmacology and Therapeutics, Ulsan University College of Medicine, Asan Medical Center) ;
  • Choi, Songie (The K-herb Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Yong-ung (Department of Pharmaceutical Engineering, College of Biomedical Science, Daegu Haany University) ;
  • Park, Gunhyuk (The K-herb Research Center, Korea Institute of Oriental Medicine)
  • 고아라 (울산대학교 의과대학 서울아산병원 임상약리학과) ;
  • 최송이 (한국한의학연구원 K-herb연구단) ;
  • 김용웅 (대구한의대학교 의과학대학 제약공학과) ;
  • 박건혁 (한국한의학연구원 K-herb연구단)
  • Received : 2016.01.08
  • Accepted : 2016.03.14
  • Published : 2016.03.30
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Abstract

Skin damage is mainly caused by environmental factors such as ultraviolet light, heat, and smoking. It is known that reactive oxygen species production is commonly involved in the pathogenesis of skin damage induced by these factors, causing skin aging. Pyrus pyrifolia Nakai continues to be a popular and highly consumed fruit in many countries with known beneficial effects including antitumor, antioxidative, and anti-inflammatory effects. However, there is no evidence of a therapeutic effect of Pyrus pyrifolia extract (PPE) against skin aging via inhibition of mitochondria-mediated apoptosis. In this study, we investigated PPE protective effect against photoaging induced by UVB ($50mJ/cm^2$) in HS68 human dermal fibroblasts. Lactate dehydrogenase assay showed that PPE significantly protected HS68 cells against UVB-induced damage in a dose-dependent manner. Other assays using DCF-DA demonstrated that PPE protected HS68 cells by regulating reactive oxygen species production. PPE also regulated mitochondrial dysfunction and mitochondrial membrane potential induced by UVB, and inhibited UVB-induced caspase-3 activity. These results indicate that PPE protects human dermal fibroblasts from UVB-induced damage by regulating the oxidative defense system.

피부 손상은 주로 자외선, 열, 담배 등과 같은 환경적 요인으로부터 초래되는데, 이는 활성산소종의 과생성으로 인한 피부노화와 연관이 있는 것으로 알려져 있다. 돌배(Pyrus pyrifolia NAKAI)는 전 세계적으로 많이 소비되는 과일로써 항암, 항산화, 항염증효과가 알려져 있다. 본 연구에서는 돌배나무잎 추출물(Pyrus pyrifolia leaf extract, PPE)의 ultraviolet B (UVB)스트레스에 대한 피부 섬유아세포 보호효과를 검증하였다. Lactate dehydrogenase assay와 DCF-DA를 이용한 정성분석 실험은 PPE가 인간의 섬유아세포에서 UVB 스트레스에 의해 유발된 세포독성 및 과생성된 활성산소종을 농도 의존적으로 억제할 뿐만 아니라, 미토콘드리아 기능저하, 막전위 저하, 그리고 세포사멸과정의 핵심 인자인 caspase-3 활성도 유의하게 억제함을 보여주었다. 결론적으로, PPE는 UVB스트레스에 의해 과생성된 활성산소종을 억제시켰으며, 이로 인해 생기는 피부세포 사멸을 효과적으로 저해함을 확인하였다.

Keywords

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