• 한국축산식품학회지
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• 제35권5호
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• pp.597-603
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• 2015

The purpose of this study was to investigate the influence of processing conditions (temperature and time) and binding agent types (glutinous rice flour, potato starch, bean flour, and acorn flour) on the physicochemical and sensory characteristics of ground dried-pork meat product. For this purpose, ground dried-pork meat product was produced by adding several binding agents at different drying temperatures and times. The drying time affected moisture content and water activity in all drying temperature. However, under the similar drying conditions, the extent of drying varied depending on the type of binding agents. The results of sensory evaluation for texture degree and overall acceptability indicated the following: overall, higher drying temperatures and longer drying time heightened the degree of texture, and the overall acceptability varied depending on binding agent type. Physicochemical and sensory characteristics were analyzed to determine any possible correlation. The results revealed a high correlation between moisture content, water activity, shear forces, and sensory evaluation (p<0.01). However, there was no correlation with respect to overall acceptability.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1459-1464
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• 2004

The purpose of this research was to investigate the potential use of cheese whey protein (CWP), a cheese by-product. The physiological activity of calcium-binding peptides in CWP may be used as a food additive that prevents bone disorders. This research also examined the characteristics of calcium-binding peptides. After the CWP was heat treated, it was hydrolyzed by trypsin. Then calcium-binding peptides were separated and purified by ion-exchange chromatography and reverse phase HPLC, respectively. To examine the characteristics of the purified calcium-binding peptides, amino acid composition and amino acid sequence were analyzed. Calcium-binding peptides with a small molecular weight of about 1.4 to 3.4 kDa were identified in the fraction that was flowed out from 0.25 M NaCl step gradient by ion-exchange chromatography of tryptic hydrolysates. The results of the amino acid analysis revealed that glutamic acid in a calcium-binding site took up most part of the amino acids including a quantity of proline, leucine and lysine. The amino acid sequence of calcium-binding peptides showed Phe-Leu-Asp-Asp-Asp-Leu-Thr-Asp and Ile-Leu-Asp-Lys from $\alpha$-LA and Ile-Pro-Ala-Val-Phe-Lys and Val-Tyr-Val-Glu-Glu-Leu-Lys from ${\beta}$-LG.

• Journal of Korean Dental Science
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• 제4권1호
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• pp.6-13
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• 2011

Purpose: Craniosynostosis (CS), one of the most common congenital craniofacial deformities, is the premature closure of cranial sutures. NELL-1 is a novel molecule overexpressed during premature cranial suture closure in human CS. From a functional perspective, NELL-1 has been reported to accelerate chondrocyte maturation and modulate calvarial osteoblast differentiation and apoptosis pathways. The mechanism through which NELL-1 induces these phenomena, however, remains unclear. The purpose of this study is to identify the NELL-1 binding protein(s) through which the biologic mechanism of NELL-1 can be further investigated. Materials and Methods: Far-Western and Immunoprecipitation (IP) assays were performed, independently and in sequence, followed by mass spectrometry to identify the NELL-1 binding proteins. Reverse IP was used to verify and confirm candidate binding protein. Results: The only confirmative protein from current experimentation was vimentin. Vimentin is the major structural component of the intermediate filaments. Conclusion: The present study identified and confirmed vimentin as a NELL-1 binding protein, which opened up a new window to mechanistically facilitate studies on this CS-associated molecule.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2006년도 추계 학술발표회 논문집
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• pp.537-540
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• 2006

Over the past few decades, a considerable number of studies on the durability of concrete have been carried out extensively. A lot of improvements have been achieved especially in modeling of ionic flows. However, the majority of these researches have not dealt with the chloride binding isotherm based on the mechanism, although chloride binding capacity can significantly impact on the total service life of concrete under marine environment. The purpose of this study is to develop the model of chloride binding isotherm based on the individual mechanism. It is well known that chlorides ions in concrete can be present; free chlorides dissolved in the pore solution, chemical bound chlorides reacted with the hydration compounds of cement, and physical bound attracted to the surface of C-S-H grains. First, sub-model for water soluble chloride content is suggested as a function of pore solution and degree of saturation. Second, chemical model is suggested separately to estimate the response of binding capacity due to C-S-H and Friedel's salt. Finally, physical bound chloride content is estimated to consider a surface area of C-S-H nano-grains and the distance limited by the Van der Waals force. The new model of chloride binding isotherm suggested in this study is based on their intrinsic binding mechanisms and hydration reaction of concrete. Accordingly, it is possible to characterize chloride binding isotherm at the arbitrary stage of hydration time and arbitrary location from the surface of concrete. Comparative study with experimental data of published literature is accomplished to validity this model.

• 한국건축시공학회지
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• 제20권5호
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• pp.459-469
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• 2020

이 연구의 목적은 철근콘크리트 기둥에서 띠철근 상세로 이용되는 갈고리 양단 135° 교차시공에 대해 동등이상의 구조적 성능을 확보하면서, 동시에 시공성을 개선할 목적으로 클립형 연결장치를 이용하여 90° 갈고리 띠철근에 결속하는 방법을 제시하였으며, 제안한 클립형 연결장치에 따른 시공성 평가 및 소요비용을 분석하였다. 분석 결과, 기둥 1개 전체 철근 조립하는 데 작업 시간은 클립형 연결장치 사용시 50%의 작업시간을 단축하는 것으로 나타났으며, 비용분석 결과 클립형 연결장치 1개 사용시 절감효과가 가장 효과적이었고, 표준 갈고리 상세 대비 약 32%의 비용 절감효과가 있는 것으로 분석되었다.

• 인지과학
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• 제18권1호
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• pp.69-90
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• 2007

본 연구의 목적은 시각체계가 색채-운동 속성의 결합에 양안시차 정보를 이용하는지를 검증하는 것이었다. Wu, Kanai와 Shimojo (2004)는 주변시야에 제시되는 색채와 운동 속성이 잘못 결합되는 색채-운동 착각 결합 현상을 보고하였는데, 본 연구는 이러한 착각 결합이 양안시차 정보가 가용하지 않은 상황에서 관찰되며 양안시차 정보가 가용한 상황에서는 색채와 운동 속성이 제대로 결합됨을 발견하였다. 이러한 실험결과는 시각체계가 속성들의 결합에 양안시차 정보를 유용하게 사용하며 속성들의 결합이 양안시차 정보가 처리되는 이후의 단계에서 발생함을 시사한다.

• Journal of Periodontal and Implant Science
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• 제46권1호
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• pp.2-9
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• 2016

Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis, and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.

• Restorative Dentistry and Endodontics
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• 제22권1호
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• pp.170-180
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• 1997

Cariogenicity of the bacteria is attributed to their binding capacity to the teeth. Bacterial attachment to oral surfaces is an essential step for colonization and subsequently infection. Therefore, it is conceivable that caries prevention can be achieved fundamentally by inhibition of bacterial attachment. The rationale for caries prevention through the use of sugar substitutes or limited use of sugar has been revealed. Among many sugar substitutes, xylitol has been shown to exhibit the most profound cariostatic effect, inhibiting glucose metabolism and possibly binding of mutans streptococci. The purpose of this study was to examine the effect of xylitol on binding of different species of oral bacteria. The effect of xylitol on binding of [$^3H$]-labeled oral bacteria to hydroxyapatite coated with human saliva(SHA) as a model for the pellicle-coated tooth surfaces was investigated. The strains of oral bacteria used in this study were A. viscosus T14V, A. viscosus WVU627, P. gingivaiis 2561, P. gingivalis A7Al-28, S. gordonii G9B, S. gordonii Challis, S. sobrinus 6715, S. mutans UA101, S. mutans KPSK -2, S. mutans T8, and S. mutans UA130. The obtained results were as follows: 1. P. gingivalis A7 Al-28, S. mutans UA130, S. mutans T8 grown with xylitol showed greater binding to SHA than the organism grown without xylitol. Among these, S. mutans T8 showed the greatest rate of increase in its binding to SHA ; 8-fold increase in its binding with xylitol. 2. S. mutans KPSK -2 grown with xylitol showed 2 times lesser binding to SHA than the organism grown without xylitol. 3. Binding ability of the remaining strains grown with xylitol to SHA was almost same as that of the organisms grown without xylitol. The overall results suggest that use of xylitol in the oral cavity may affect the complex oral bacterial ecosystem.

• 인터넷정보학회논문지
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• 제8권4호
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• pp.81-91
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• 2007

MIPv6에서 경로 최적화를 제공하기 위한 바인딩 갱신은 다양한 공격에 취약할 수 있다. 따라서 안전한 바인딩 갱신이 MIPv6의 중요한 연구 과제가 되었으며, 이를 위한 여러 프로토콜들이 제안되었다. 본 연구에서는 MIPv6가 기본적으로 제공하는 RR과 기존의 대표적인 프로토콜인 SUCV와 OMIPv6를 상세히 분석하여 MN의 암호화 연산 및 각 프로토콜의 공격 취약성, 주소 생성 및 관리와 확장성의 문제점을 제시하고, 안전한 바인딩 갱신을 위한 설계 목표를 제시한다. 이를 근거로 새로운 바인딩 갱신 프로토콜을 제안한다. 제안 프로토콜은 기존 프로토콜들보다 다양한 공격에 대한 방어를 강화하며, 주소관리와 확장성을 개선하고, 위치 보안성을 제공하며, 제한된 계산 능력을 갖는 MN의 연산 부하를 감소시킨다.

• 한국구조물진단유지관리공학회 논문집
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• 제24권4호
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• pp.72-80
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• 2020

이 연구의 목적은 철근콘크리트 기둥에서 외부띠철근 상세로 이용되는 갈고리 양단 135도 교차 시공 상세에 대해, 대등한 구조적 성능을 확보하면서, 동시에 시공성을 개선할 목적으로 클립형 연결장치로 띠철근 갈고리 보강하는 방법을 제시하였다. 제안한 클립형 연결장치로 결속한 띠철근의 정착거동 및 강도를 파악하기 위해 콘크리트 압축강도, 클립형 연결장치의 묻힘길이, 설치 위치를 주요 변수로 28개 인발 실험체를 제작하여 실험하였다. 실험결과, 정착강도는 콘크리트 압축강도, 띠철근 직경, 클립의 묻힘길이, 설치 위치에 관계없이 클립형 연결장치로 결속하는 경우, 표준 갈고리 상세보다 모두 높게 나타났고 표준 갈고리 상세의 정착거동과 유사한 거동을 보여주었다. 그러므로, 90도 갈고리에 클립형 연결장치로 결속하는 상세는 표준 갈고리 상세와 대등한 정착거동 및 성능을 발휘할 수 있는 것으로 평가할 수 있다.