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http://dx.doi.org/10.5051/jpis.2016.46.1.2

Surface interactions between two of the main periodontal pathogens: Porphyromonas gingivalis and Tannerella forsythia  

Zhu, Weidong (Department of Medicine, University of California School of Medicine)
Lee, Seok-Woo (Departments of Dental Education and Periodontology, Dental Science Research Institute, Chonnam National University School of Dentistry)
Publication Information
Journal of Periodontal and Implant Science / v.46, no.1, 2016 , pp. 2-9 More about this Journal
Abstract
Purpose: Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. Methods: Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia. Results: It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis, and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells. Conclusions: LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.
Keywords
Bacterial outer membrane proteins; Biotinylation; Periodontitis; Porphyromonas gingivalis; Tannerella forsythia;
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1 Kolenbrander PE. Coaggregations among oral bacteria. Methods Enzymol 1995;253:385-97.
2 Kolenbrander PE, Andersen RN, Blehert DS, Egland PG, Foster JS, Palmer RJ Jr. Communication among oral bacteria. Microbiol Mol Biol Rev 2002;66:486-505.   DOI
3 Kirschbaum M, Schultze-Mosgau S, Pfister W, Eick S. Mixture of periodontopathogenic bacteria influences interaction with KB cells. Anaerobe 2010;16:461-8.   DOI
4 Kolenbrander PE. Oral microbial communities: biofilms, interactions, and genetic systems. Annu Rev Microbiol 2000;54:413-37.   DOI
5 Kesavalu L, Sathishkumar S, Bakthavatchalu V, Matthews C, Dawson D, Steffen M, et al. Rat model of polymicrobial infection, immunity, and alveolar bone resorption in periodontal disease. Infect Immun 2007;75:1704-12.   DOI
6 Zambon JJ. Periodontal diseases: microbial factors. Ann Periodontol 1996;1:879-925.   DOI
7 Kamma JJ, Nakou M, Manti FA. Predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis. J Periodontal Res 1995;30:66-72.   DOI
8 Yoneda M, Hirofuji T, Anan H, Matsumoto A, Hamachi T, Nakayama K, et al. Mixed infection of Porphyromonas gingivalis and Bacteroides forsythus in a murine abscess model: involvement of gingipains in a synergistic effect. J Periodontal Res 2001;36:237-43.   DOI
9 Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol 2010;8:481-90.   DOI
10 Takemoto T, Kurihara H, Dahlen G. Characterization of Bacteroides forsythus isolates. J Clin Microbiol 1997;35:1378-81.
11 Yoneda M, Yoshikane T, Motooka N, Yamada K, Hisama K, Naito T, et al. Stimulation of growth of Porphyromonas gingivalis by cell extracts from Tannerella forsythia. J Periodontal Res 2005;40:105-9.   DOI
12 Sabet M, Lee SW, Nauman RK, Sims T, Um HS. The surface (S-) layer is a virulence factor of Bacteroides forsythus. Microbiology 2003;149:3617-27.   DOI
13 Yao ES, Lamont RJ, Leu SP, Weinberg A. Interbacterial binding among strains of pathogenic and commensal oral bacterial species. Oral Microbiol Immunol 1996;11:35-41.   DOI
14 Um HS, Lee SW, Park JH, Nauman RK. Coaggregation between Porphyromonas gingivalis and Tannerella forsythia . J Korean Acad Periodontol 2006;36:265-72.   DOI
15 Potempa J, Sroka A, Imamura T, Travis J. Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis : structure, function and assembly of multidomain protein complexes. Curr Protein Pept Sci 2003;4:397-407.   DOI
16 Kamaguchi A, Ohyama T, Sakai E, Nakamura R, Watanabe T, Baba H, et al. Adhesins encoded by the gingipain genes of Porphyromonas gingivalis are responsible for co-aggregation with Prevotella intermedia. Microbiology 2003;149:1257-64.   DOI
17 Shimotahira N, Oogai Y, Kawada-Matsuo M, Yamada S, Fukutsuji K, Nagano K, et al. The surface layer of Tannerella forsythia contributes to serum resistance and oral bacterial coaggregation. Infect Immun 2013;81:1198-206.   DOI
18 Lee SW, Sabet M, Um HS, Yang J, Kim HC, Zhu W. Identification and characterization of the genes encoding a unique surface (S-) layer of Tannerella forsythia. Gene 2006;371:102-11.   DOI
19 Yoo JY, Kim HC, Zhu W, Kim SM, Sabet M, Handfield M, et al. Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease. FEMS Microbiol Lett 2007;275:344-52.   DOI
20 Finlay BB, Falkow S. Common themes in microbial pathogenicity revisited. Microbiol Mol Biol Rev 1997;61:136-69.
21 Marsh PD. Dental plaque: biological significance of a biofilm and community life-style. J Clin Periodontol 2005;32 Suppl 6:7-15.   DOI
22 Marsh PD, Moter A, Devine DA. Dental plaque biofilms: communities, conflict and control. Periodontol 2000 2011;55:16-35.   DOI
23 Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83.   DOI
24 Marsh PD. Dental plaque: biological significance of a biofilm and community life-style. J Clin Periodontol 2005;32 Suppl 6:7-15.   DOI
25 Socransky SS, Haffajee AD. Evidence of bacterial etiology: a historical perspective. Periodontol 2000 1994;5:7-25.   DOI
26 Kroes I, Lepp PW, Relman DA. Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci USA 1999;96:14547-52.   DOI
27 Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005;43:5721-32.   DOI
28 Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol 1998;25:134-44.   DOI
29 Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994;5:78-111.   DOI