• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11b
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• pp.233-235
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• 2003

한국 서해안과 남해안 47개소의 해수와 mud 시료로부터 광합성세균으로 유추되는 13개의 균주를 분리하였고, agar-shake tube method와 RAPD-PCR을 이용하여 4개 서로 다른 균주를 순수분리 하였다. 4개의 균주 중 폐수분해능이 가장 뛰어난 균을 선정하여, 형태학적, 배양적, 생화학적 특성 및 16S rRNA sequencing에 의한 동정결과 purple, sulfur bacteria 쪽에 가까웠으나 형태학적, 배양학적, 생화학적 특성이 purple, non-sulfur bacteria의 Rhodobacter 속에 가장 근접하여, Rhodobacter sp. EGH-24로 명명하였다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2002.05b
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• pp.450-451
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• 2002

미래산업의 중요한 자원인 광합성세균(purple non-sulfur photosynthetic bacteria)은 해양에 대한 연구정도가 미비하기 때문에 이 연구를 수행하였다. 현재 총 47개의 mud 시료중에서 15개의 광합성세균으로 추정되는 strain을 분리하였다. 이후의 실험은 RAPD PCR을 이용하여 중복되는 strain의 유무를 확인하고, 165 rDNA의 염기분석을 통하여 분류학적인 위치를 연구할 계획이다.

• 한국신재생에너지학회:학술대회논문집
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• 2009.06a
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• pp.782-786
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• 2009

Thiocapsa roseopersicina NCIB 8347은 purple sulfur bacteria이며 광합성종속영양 조건에서는 nitrogenase 효소계가 유도되어 질소를 고정하며, 수소를 발생한다. 또한 광합성독립영양 조건에서는 hydrogenase 효소계가 유도되어 3~4개 종류의 특성이 다른 hydrogenase가 membrane에 결합되어 있거나, cytoplasma에 존재하며, 이 중의 일부는 산소농도와 온도의 상승에도 비교적 안정하다. 본 연구에서는 T. roseopersicina NCIB 8347이 광합성종속영양 조건에서 수소를 생산할 수 있는 제반 배양조건을 최적화하고, nitrogenase와 일부 hydrogenase역가를 측정하여 purple non-sulfur bacteria, Rhodobacter sphaeroides KD131의 nitrogenase와 비교하여 수소생산을 최적화하였다. 할로겐램프를 8-9 $Klux/m^2$로 조사할 때와 배양온도 $26{\sim}30^{\circ}C$, 배양시간 72시간에서 균체 성장과 수소생산이 가장 높았다. T. roseopersicina NCIB 8347는 광합성 독립영양, 종속영양 조건에서 모두 성장 할 수 있었다.

• Journal of Microbiology and Biotechnology
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• v.24 no.8
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• pp.1034-1043
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• 2014

This study determined the effect of light intensity and photoperiod on the dry cell weight and total amount of carotenoids in four isolates of purple non-sulfur bacteria obtained from shaded and exposed microhabitats of a mangrove ecosystem in Kota Kinabalu, Sabah, Malaysia. The initial isolation of the bacteria was carried out using synthetic 112 medium under anaerobic conditions (2.5 klx) at $30{\pm}2^{\circ}C$. On the basis of colony appearance, cell morphology, gram staining, motility test, and 16S rRNA gene sequencing analyses, all four bacteria were identified as Afifella marina. One of the bacterial isolates, designated as Af. marina strain ME, which was extracted from an exposed mud habitat within the mangrove ecosystem, showed the highest yield in dry cell weight ($4.32{\pm}0.03g/l$) as well as total carotenoids ($0.783{\pm}0.002mg/g$ dry cell weight). These values were significantly higher than those for dry cell weight ($3.77{\pm}0.02g/l$) and total carotenoid content ($0.706{\pm}0.008mg/g$) produced by the isolates from shaded habitats. Further analysis of the effect of 10 levels of light intensity on the growth characteristics of Af. marina strain ME showed that the optimum production of dry cell weight and total carotenoids was achieved at different light intensities and incubation periods. The bacterium produced the highest dry cell weight of 4.98 g/l at 3 klx in 72 h incubation, but the carotenoid production of 0.783 mg/g was achieved at 2.5 klx in 48 h incubation. Subsequent analysis of the effect of photoperiod on the production of dry cell weight and total carotenoids at optimum light intensities (3 and 2.5 klx, respectively) revealed that 18 and 24 h were the optimum photoperiods for the production of dry cell weight and total carotenoids, respectively. The unique growth characteristics of the Af. marina strain ME can be exploited for biotechnology applications.

• Korean Journal of Microbiology
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• v.29 no.2
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• pp.130-135
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• 1991

The effects of a herbicide butachlor[2-chloro-2', 6'-diethyl-N-(butoxymethyl) acetanilide] on the growth of the purple non-sulfur bacteria were investigated. The butachlor inhibited the growth of all species tested by 18-51%, except Rhodospirillum rubrum at concentrations of M, which would be field capacity. The photosynthetic growth rate of the species in the presence of butachlor was influenced by the nitrogen source. Cultures supplied with (NH&S04 showed a somewhat higher growth rate than those fixing dinitrogen, but they were more susceptible to butachlor (26-51%). On the contrary, butachlor enhanced the growth rate of Rhodospirillum rubrum in nitrogen gas conditions. When the culture was performed in medium with butachlor as the carbon source, the cells of fixing dinitrogen showed a higher exhaustion of butachlor than those supplemented with (N&)2S04, which exhaustion was examined by a decrease of the major absorbance at 213 nm and 260 nm. The exhaustion of butachlor as the carbon source had relation with the growth of the cells. The alkalization of culture supplemented with nitrogen gas was found in the cells treated with butachlor or untreated. The butachlor affected the carotenoid region but bacteriochlorophyll remained unaffected.

• Environmental Engineering Research
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• v.19 no.3
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• pp.293-298
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• 2014

An anaerobic photosynthetic treatment process utilizing purple non-sulfur photosynthetic bacteria (PNSB) was applied to the recovery of organic carbon from food processing wastewater. PNSB cells, by-product from the treatment, have high nutrition such as proteins and vitamins which are a good alternative for fish feed. Effects of light source on performance of anaerobic photosynthetic process were investigated in this study. Two bench-scale photo-bioreactors were lighted with infrared light emitting diodes (LEDs) and tungsten lamps covered with infrared transmitting filter, respectively, aiming to supply infrared light for photosynthetic bacteria growth. The photo-bioreactors were operated to treat noodle-processing wastewater for 323 days. Hydraulic retention time (HRT) was set as 6 days. Organic removals in the photo-bioreactor lighted with infrared LEDs (91%-95%) was found higher than those in photo-bioreactor with tungsten lamps with filter (79%-83%). Biomass production in a 150 L bench-scale photo-bioreactor was comparable to a 8 L small-scale photo-bioreactor in previous study, due to improvement of light supply efficiency. Application of infrared LEDs could achieve higher treatment performance with advantages in energy efficiency and wavelength specifity.

• The Korean Journal of Food And Nutrition
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• v.10 no.4
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• pp.549-552
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• 1997

A purple non-sulfur photosynthetic bacteria which evolved molecular hydrogen efficiently from glucose in the presence of low concentration of NH4+ under light illuminated anaerobic condition was isolated from mud samples in Korea. This bacteria was identified on Rhodobacter sphaeroides KS 56 based on the morphological, cultural and physiological characteristics.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.189-197
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• 2002

Twenty nine strains of photosynthetic purple nonsulfur bacteria were isolated from Kyonggi area in Korea. The isolated strains were identified as Rhodopseudomonas blastica, Rhodocyclus gelatinosus, Rhodocyclus tenuis, and Rhodopseudomonas rutila. The enhanced nutrients removal system for wastewater using phototrophic purple non-sulfur bacteria was developed. Experiments were performed into two Phases and the results were compared: the synthetic wastewater was tested for the removal efficiency of nutrients and organics during Phase 1 and the real wastewater during Phase2. Results showed that 97∼99％ of organics were removed during Phase 1 and 96∼99％ during Phase 2. Nutrients (nitrogen and phosphorus) were also removed efficiently: 85∼91％ removal of T-N and 78∼92％ removal of T-P were achieved for Phase 1, and 76∼89％ removal of T-N and 73∼88％ removal of T-P for Phase 2.

• KSBB Journal
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• v.20 no.6
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• pp.393-400
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• 2005

Hydrogen($H_2$) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological $H_2$ production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological $H_2$ production processes, that are being explored in fundamental and applied research, are reviewed.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.409-411
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• 2003

A species of facultative photo-organotrophic, purple, non-sulfur bacterium was isolated from the west coast and the south coast 47 area of Korea at 2001 September. Separated 13 samples of changes with red color under $28{\sim}32\;^{\circ}C$, 3000 lux, anaerobe conditions for 7 days cultivated in Basal medium. For a pure isolation from 13 samples it used agar-shake tube method (0.4 % agar) and it separated 5 strains through 13-repetition test. The RAPD(Random Amplified Polymorphic DNA)-PCR result of strains (EGH-9, EGH-13, EGH-23, EGH-24, EGH-30) that EGH-24 and EGH-30 was same strain. For wastewater biodegradation test that 4 isolation strains cultivated in synthesis wastewater in 7 days. EGH-24 was high 63000 mg/L (CODcr) to 43400 mg/L (CODcr). EGH-24 was selected with efficient wast water treated strain. Based on the results obtained from morphology, nutrient requirements, major bacteriochlorophyll content, 165-rDNA phylogenetic analysis, this strain may be identified as a new strain of the genus Rhodobacter and named Rhodobacter sp. EGH-24.