• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.196-204
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• 2010

With the completion of genome sequencing of several organisms, attention has been focused to determine the function and functional network of proteins by proteome analysis. The recent techniques of proteomics have been advanced quickly so that the high-throughput and systematic analyses of cellular proteins are enabled in combination with bioinformatics tools. Furthermore, the development of proteomic techniques helps to elucidate the functions of proteins under stress or diseased condition, resulting in the discovery of biomarkers responsible for the biological stimuli. Ultimate goal of proteomics orients toward the entire proteome of life, subcellular localization, biochemical activities, and their regulation. Comprehensive analysis strategies of proteomics can be classified as three categories: (i) protein separation by 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification by either Edman sequencing or mass spectrometry (MS), and (iii) quanitation of proteome. Currently MS-based proteomics turns shiftly from qualitative proteome analysis by 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, to quantitative proteome analysis. Some new techniques which include top-down mass spectrometry and tandem affinity purification have emerged. The in vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes, protein-labeling tagging with isotope-coded affinity tag, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope labeled amino acid can be in vivo labeled into live culture cells through metabolic incorporation. MS-based proteomics extends to detect the phosphopeptide mapping of biologically crucial protein known as one of post-translational modification. These complementary proteomic techniques contribute to not only the understanding of basic biological function but also the application to the applied sciences for industry.

• 펄프종이기술
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• 제46권5호
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• pp.79-87
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• 2014

The enrichment with nutrients in rivers and lakes becomes serious problems in Korea. As the environmental friendly solution, the phytoremediation are getting more attention and the water hyacinth could be a strong candidate plant for this. Water hyacinth is a free floating freshwater plant proven to be a effective plant used for a purification of a polluted lakes, sewage wastewater, and livestock wastewater. The biggest obstacles for application of water hyacinth in Korea could be the disposal of the water hyacinth before winter, because there is no feasible use for the water hyacinth until now. In this study, the characteristics of water hyacinth were deeply investigated for providing background data to develop appropriate application of the water hyacinth. The water hyacinth was fractioned three parts, leaf, air blader, root of which morphological properties were evaluated with microscope and field emission scanning electron microscope (FE-SEM). The water contents of each parts of the water hyacinth were 87% for leaf, 96.5% for air bladder, 94.6% for root. The chemical compositions of each parts were also measured and showed around 29% of hot water extract in leaf and air bladder, 42% holocellulose for leaf, 47% holocellulose for air bladder. The application of water hyacinth biomass without root parts to papermaking provided bulkier structure, but the tensile strength was decreased.

• 화훼연구
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• 제19권2호
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• pp.119-125
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• 2011

창포는 한국에 자생하는 수생식물로 약용뿐만 아니라 phytoremediation과 수질정화 식물로도 알려져 있다. 본 연구에서는 창포의 기내 식물체에 다양한 선량(20-500 Gy)의 감마선을 조사하여, 방사선의 효과 및 감수성을 확인하기 위해 2달후 생존율, 신초형성율 및 생장율을 측정하였다. 생존율과 신초형성율은 감마선 선량이 증가할수록 현저하게 감소하였다. 기내에서 저선량을 조사한 식물체의 생장율은 대조구에 비해 오히려 증가하였으나, 250 Gy 이상에서는 생장이 멈추거나 유의하게 감소하였으며, 반치사선량($LD_{50}$)은 240 Gy로 다른 식물종의 $LD_{50}$보다 훨씬 높은 선량이었다. 창포에서 저선량을 조사하였을 때 생장율이 증가하는 방사선호메시스(radiation hormesis) 효과를 확인하였다.

• Journal of Ecology and Environment
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• 제42권4호
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• pp.183-190
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• 2018

Backgrounds: The main purpose of this research was to assess changes in vegetation structure, wetland index, and diversity index for a 15-year-old created wetland in Jincheon, South Korea. The created wetland consists of four sub-wetlands: a kidney-shaped wetland, a ditch, an ecological pond, and a square wetland. Vegetation and water depth data were collected at each site in 1999 and 2013, and Shannon diversity and wetland indices were calculated. Results: The total number of plant species increased from 18 in 1999 to 50 in 2013, and the ecological pond in 1999 and the ditch in 2013 presented the highest diversity indices (2.5 and 3.2, respectively). Plant species were less diverse in 1999 than in 2013, presumably because these initial wetlands were managed periodically for water purification and installation of test beds. The proportion of wetland plants, including obligate wetland and facultative wetland species, decreased from 83 to 56%, whereas upland plants, including obligate upland and facultative upland species, increased from 17 to 44%. After ceasing water supply, water depth in all four sub-wetlands declined in 2013. Thus, upland plants established more readily at these sites, resulting in higher diversity and lower wetland indices than in 1999. Conclusions: The major floristic differences between 1999 and 2013 were an increase in the number of upland plants and a decrease in wetland species. Although wetland indices were lower in 2013, the created wetland performed important ecosystem functions by providing habitats for wetland and upland plants, and the overall species diversity was high.

• 한국물환경학회지
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• 제38권6호
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• pp.267-274
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• 2022

This paper is a result of research conducted on the 800,000 m³/d capacity of A Water Treatment Plant (WTP) and 400,000 m³/d capacity of B WTP plant in operation in the Nakdong River region. We evaluated the effect of algae broom on the WTP operation based on the running data of both WTP and the data on the pre-oxidation process field test for algae control using sodium permanganate (SPM) at the B WTP. The study results showed that during the algal bloom period, the coagulant dose increased by 102% in A WTP and 58% in B WTP, respectively, and the chlorine dose also increased by 38% and 29%, respectively, which may affect Total trihalomethane (THM) production. Data such as algal populations and Chl-a, residual chlorine and THM, algal populations, and ozone dose appeared also highly correlated, confirming that algal broom affects WTP operations, including water quality and chemical dosage. As a result of the field test of B WTP, THMs appeared lower than that of the control, suggesting the possibility of the SPM pre-oxidation process as an alternative to algae-related water quality management. Furthermore, in terms of GHG emissions due to energy consumption, it was observed that the pre-oxidation process using SPM was approximately 10.8%, which is a very low ratio compared to the pre-ozonation process. Therefore, these results suggest that the SPM pre-oxidation process can be recommended as an alternative to low-carbon water purification technology.

• 한국환경농학회지
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• 제22권1호
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• pp.26-35
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• 2003

농어촌 등에서 소규모로 발생하는 하수를 환경친화적이고 높은 하수처리효율을 유지하면서 하수처리장의 장기간 사용을 위한 최적의 여재를 선정하기 위하여 자연정화공법을 이용한 소형하수처리장치를 호기성조 및 혐기성조로 구분하여 시공한 다음, 하수처리량 및 여재 입경별 수처리 효율을 조사하였고, 호기성조와 혐기성조에서 여재 입경별 하수의 투수속도를 조사한 결과는 다음과 같다. 호기성조 처리수 및 방류수중의 pH 및 EC는 여재입경에 따라서 별 차이가 없었고, 용존산소는 호기성조를 통과한 호기성조 처리수의 용존산소는 큰 폭으로 증가하였으나 혐기성조를 통과한 방류수의 용존산소는 호기성조 처리수에 비해 약간 감소하여 여재입경 및 하수처리량에 따라서 별 차이가 없이 약 $2.4{\sim}5.1\;mg/L$정도이었다. BOD, COD 및 탁도 처리율은 여재 대($4{\sim}10\;mm$)를 사용했을 경우에도 호기성조 처리 수에서 BOD 처리율은 약 91%이상 COD 처리율은 73%이상, 탁도 처리율은 83%이상이었으며, 이들 처리율은 여재입경이 작을수록 증가하였고, 모든 조건에서 방류수중의 BOD 처리율은 98%이상, COD 처리율은 91%이상, 탁도 처리율은 98%이상이었다. 여재입경별 총 질소 및 총 인 처리율은 여재입경이 작을수록 약간 증가하였고, 모든 조건에서 방류수중의 총 질소 처리율은 약 $45{\sim}59%$, 총 인 처리율은 약 $80{\sim}96%$ 정도이었다. 하수 처리율 및 투수속도를 고려해 볼 때 하수처리장 호기성조의 최적입경은 $2{\sim}4\;mm$정도, 혐기성조의 최적입경은 $0.1{\sim}4\;mm$정도가 적절한 것으로 사료되었다. 따라서 이러한 조건을 자연정화공법을 이용한 하수처리장에 적용하면 높은 하수처리효율을 유지하면서 하수처리장의 공극폐쇄현상이 일어나지 않아 장기간 운전할 수 있을 것으로 판단된다.

• 환경영향평가
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• 제26권6호
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• pp.508-523
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• 2017

본 연구는 매노천 생태하천복원을 위한 생태적수질정화비오톱 시스템과 생태어도(Fish-way) 등의 적용 대상지를 중심으로 생태하천 복원 전 후를 모니터링하였다. 어류는 복원전 서식이 확인되지 않았던 생태적수질정화비오톱 습지에서 총 11종 191개체가 창출복원된 것으로 조사되었다. 특히 복원목표종인 돌마자와 참종개가 생태적수질정화비오톱 습지에서 서식함이 모니터링되어, 미소서식처와 건전한 Fish-way가 창출되었음을 확인할 수 있었다. 양서류는 복원후 활동성이 높은 3차 조사시기에 습지와 그 주변에서 다수의 참개구리 서식으로 복원되었음이 확인되었다. 포유류는 수달이 습지와 Fish-way를 서식영역으로 활용하는 것이 확인되어 환경부 멸종위기 제1급이자 천연기념물인 수달 서식지가 복원된 것으로 조사되었다. 식물상의 경우 조사지역에 출현하는 관속식물은 복원 전 총 7과 13종, 복원직후 15과 19종, 복원후 총 22과 33종으로 증가되었다. 복원후 식생은 달뿌리풀 군락 등의 다양한 생태계의 기초생산자이자 수질정화에 기여하는 식재종인 정수식물군락이 형성된 것으로 조사되었다. 수질 모니터링 결과, 평균적으로 BOD 64.3%, T-N 47.2%, T-P 80.7%의 처리효율을 나타내었다. 생태계를 교란하는 제한요인(limiting factor)이 되는 비점오염원이 성공적으로 처리됨으로써 I, II등급수질에 서식하는 목표종이 창출적으로 복원되었다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1987년도 식물생명공학 심포지움 논문집 Proceedings of Symposia on Plant Biotechnology
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• pp.133-148
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• 1987

Gibberellin(GA) 3-$\beta$ hydroxylation is very important for the shoot elogation in the higher plants, since only 3$\beta$-hydryoxylated GAs promote shoot elogation in several plants. Fluctuation of 3$\beta$-hydryoxylase activity was examined during seed maturation using two cultivars of , P. vulgaris, Kentucky Wonder (normal) and Masterpiece (dwarf). Very immature seeds of both cultivars contain high level of 3$\beta$-hydroxylase activity (per mg protein). Both cultivars showed maximum of enzyme activity (per seed) in the middle of their maturation process. Gibberellin 3$\beta$-hydroxylase catalyzing the hydroxylation of GA20 to GA1 was purified 313-fold from very early immature seeds of P. vulgaris. Crude soluble enzyme extracts were purified by 15% methanol precipitation, hydrophobic interaction chromatogrphy, DEAE ion exchange column chromatography and gel filtration HPLC. The 3$\beta$-hydroxylase activity was unstable and lost much of its activity duting the purification. The molecular weight of purified enzyme was extimated to be 42, 000 by gel filtration HPLC and SDS-PAGE. The enzyme exhibited maximum activity at pH 7.7. The Km values for [2.3-3H] GA20 and [2.3-3H]GA9 were 0.29 $\mu$M and 0.33 $\mu$M, respectively. The enzyme requires 2-oxoglutarate as a cosubstrate; the Km value for 2-oxoglutarate was 250 $\mu$M using 3H GA20 as a substrate. Fe2+ and ascorbate significantly activated the enzyme at all purification steps, while catalase and BSA activated the purified enzyme only. The enzyme was inhibited by divalent cations Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+. Effects of several GAs and GA anaogues on the putrified 3$\beta$-hydroxylase were examined using [3H]GA9 and GA20 as a substrates. Among them, GA5, GA9, GA15, GA20 and GA44 inhibited the enzyme activity. [13C, 3H] GA20 was converted by the partially purified enzyme preparation to [13C, 3H]GA1, GA5 and GA6, which were identified by GC-MS, GA9 was converted only GA4, GA15 and GA44 were converted to GA37 and GA38, respectively. GA5 was epoxidized to GA6 by the preparation. This suggests that 3$\beta$-hydroxylation of GA20 and epoxidation of GA5 are catalyzed by the same enzyme in P, vulgaris.

• 한국환경농학회지
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• 제27권1호
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• pp.18-26
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• 2008

본 연구는 농촌전원독립가구 하수처리장에서 오염물질의 존재형태별 처리 경향을 파악하여 하수처리장의 설계 및 시공 시 부지면적 감소와 오염물질의 처리효율 극대화를 위한 기초자료로 활용하기 위하여 농촌전원독립가구 하수처리장에서 하수 중 오염물질의 거동 특성과 오염물질의 존재형태별 분해속도를 각각 조사하였다. 하수 중 오염물질의 존재형태별 거동을 조사한 결과 BOD 및 COD는 대부분 호기성조에서 처리되었으며, 처리된 BOD와 COD는 대부분 insoluble형태이었다. 그러나 무산소조와 혐기성조에서의 BOD와 COD는 존재형태별로 별 차이 없었다. SS는 대부분 호기성조에서 처리되었으며, 처리된 SS는 대부분 volatile 형태이었다. T-N은 BOD, COD 및 SS와 달리 호기성조에서 처리효율이 높지 않았고, 처리된 대부분의T-N은 dissolved 형태이었다. T-P는 모든 조에서 일정하게 처리효율이 증가되었으며, 처리된 T-P는 대부분 dissolved 형태이었다. 현장 하수처리장에서 존재형태별 오염물질의 분해속도를 조사한 결과 BOD, COD 및 SS의 분해속도는 호기성조에서 각각 30.79, 17.15 및 29.96 $day^{-1}$로 무산소조 및 혐기성조에 비해 매우 빨랐으며, 호기성조에서 존재형태별 분해속도는BOD 및 COD의 경우 insoluble 형태가 빨랐으며, SS의 경우 fixed 형태가 빨랐다. T-N 및 T-P의 분해속도는 호기성조에서 각각 4.78 및 13.09 $day^{-1}$로 다른 조에 비해 약간 빨랐으며, 호기성조에서 존재형태별 분해속도는 suspended 형태가 약간 빨랐고, 혐기성조와 무산소조에서는 별 차이 없이 비슷하였다. 이상의 결과는 하수처리장의 설계 및 시공 시 부지면적 감소와 오염물질의 처리효율 극대화를 위한 기초자료로 활용 할 수 있을 것으로 판단된다.