Browse > Article

Radioresistance of Acorus calamus to Gamma Ray Irradiation  

Lee, Ja-Hyun (Major of Horticulture, Department of Plant Biotechnology, Chonnam National University)
Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
Lee, Geung-Joo (Major in Horticultural Science, Division of Bioscience, Mokpo National University)
Lee, Seong-Gene (Department of Molecular Biotechnology, Bioenergy Research Center, Chonnam National University)
Kim, Sun-Kook (Jeollanamdo Agricultural Research and Extension Services)
Han, Tae-Ho (Major of Horticulture, Department of Plant Biotechnology, Chonnam National University)
Publication Information
FLOWER RESEARCH JOURNAL / v.19, no.2, 2011 , pp. 119-125 More about this Journal
Abstract
Acorus calamus is an indigenous hydrophyte used as phytoremediation and water purification as well as medicinal herb. Irradiation of gamma ray was performed to investigate the effect on radioresistance of A. calamus on in vitro. Various doses (20-500 Gy) of gamma rays were irradiated to in vitro plantlets. The effect of irradiation was studied on survival rate, shoot formation and growth rate. The survival and multiplication rate was remarkably decreased with the increase of radiation dose. The growth of plantlets was significantly increased in comparison to the control at low doses. The hermetic effect of radiation was appeared in A. calamus. It was significantly decreased or interrupted above 250 Gy. The mean 50% lethal dose ($LD_{50}$) was extremely high as 240 Gy. We conclude that A. calamus had remarkably high radioresistance compared with other plant species.
Keywords
Acorus; radiation hormesis; in vitro culture; mutagenesis;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Abdel-Hady, M.S., E.M. Okasha, S.S.A. Soliman, and M. Talaat. 2008. Effect of gamma radiation and gibberellic acid on germination and alkaloid production in Atropa belladonna L. Aust. J. Basic Appl. Sci. 2:401-405.
2 Cox, M.M. and J.R. Battista. 2005. Deinococcus radiodurans-the consummate survivor. Natl. Rev. Microbiol. 3:882-892.   DOI   ScienceOn
3 Eun, J.S., J.S. Kim, H.S. Lim, S.K. Han, S.R. Choi, and Y.S. Jang. 2007. Effect of proton ion and gamma-ray irradiation on radiosensitivity of $M_1$ seedlings in Brassica napus . Kor. J. Hort. Sci. Technol. 25:17-23.
4 Feinendegen, L.E. 2005. Evidence for beneficial low level radiation effects and radiation hormesis. Br. J. Radiol. 78:3-7.   DOI   ScienceOn
5 Gilani, A.H., A.J. Shah, M. Ahmad, and F. Shaheen. 2006. Antispasmodic effect of Acorus calamus Linn. is mediated through calcium channel blockade. Phytother. Res. 20: 1080-1084.   DOI   ScienceOn
6 Hameed, A., T.M. Shah, B.M. Alta, M.A. Haq, and H. Sayed. 2008. Gamma irradiation effects on seed germination and growth, protein content, peroxidase and protease activity, lipid peroxidation in desi and kabu li chickpea. Pak. J. Bot. 40:1033-1041.
7 Han, W.J. 2003. Comparative effects of Radix polygalae and rhizome Acorus graminei on CT 105-induced neuroblastoma cell lines. Thesis, Wonkwang Univ. Kor.
8 Ichikawa, S. 1972. Somatic mutation rate in Trades cantia stamen hairs at low radiation levels: Finding of low doubling doses of mutations. Jap. J. Genet. 47:411-421.   DOI
9 Johansson, L. 2003. Hormesis, an update of the present position. Euro. J. Nucl. Med. Mol. Imag. 30:921-933   DOI   ScienceOn
10 Ka, M.H., E.H. Choi, H.S. Chun, and H.G. Lee. 2005. Antioxidative activity of volatile extracts isolated from Angelica tenussimae roots, peppermint leaves, pine needles, and sweet flag leaves. J. Agric. Food Chem. 53:4124-4129.   DOI   ScienceOn
11 Kim, G.J., G.C. Koh, G.Y. Gi, K.J. Choi, and H.S. Song. 2006. In vitro mutant induction by irradiation of gamma-ray in Rosa hybrida Hort. Kor. J. Hort. Sci. Technol. 24:497-502.
12 Kim, J.K., S.A. Noh, H.S. Shin, H.S. Song, and B.J. Choi. 2002. Effects of irradiation and cold storage on rooting and growth of Chrysanthemum cuttings. Kor. J. Environ. BioI. 20: 355-359.
13 Kim, J.K., Y.K. Kim, B.H . Lee, and Y.l. Lee. 1999. Effects of natural extracts on the radiation- induced pink mutations in Tradescantia stamen hair cells. J. Kor. Nucl. Soc. 31:522-528.
14 Kim, S.I., H.H. Lee, C.S. Kim, M.D. Seo, and S.Y. Lee. 2008a. Changes in the seed viability and DPPH-radical scavenging of Helianthus annuus seeds according to low dose ${\gamma}-ray$ irradiation. J. Kor. Soc. Appl. Biol. Chem. 51:148-152.   DOI   ScienceOn
15 Kim, Y.S. 2008b. Evaluation of the water purification capacity in Iris pseudacorus and Acorus calamu. Kor. J. Hort. Sci. Technol. 26:172-176.
16 Lee, H.J., G.J. Lee, D.S. Kim, J.B. Kim, J.H. Ku, and S.Y. Kang. 2008a. Selection and physiological characterization of glyphosate-tolerant zoysiagrass mutants derived from a gamma ray irradiation. Kor. J. Hort. Sci. Technol. 26:454-463.
17 Lee, I.S., D.S. Kim, H.D. Yoon, Y.P. Lim, and Y.I. Lee. 2002. In vitro selection and characterizations of gamma radiation-induced salt tolerant lines in rice. Kor. J. Plant. Biotech. 29:247-252.   DOI   ScienceOn
18 Lee, J.W., K.M. Seong, C H. Kim, S.Y Nam, K.H. Yang, and Y.W. Jin. 2008b. Low-dose gamma-radiation effect on early stage development and lifespan in various strains of Drosophila melanogasler. Kor. J. Occup. Environ. Med. 20:225-232.
19 Levin-Zaidman, S., J. Englander, E. Shimoni, A.K. Sharma, K.W. Minton, and A. Minsky. 2003. Ring like structure of the Deinococcus radiodurans genome: A key to radio-resistance? Science 299:254-256.   DOI   ScienceOn
20 Lu, G, X.Y. Zhang, Y.J. Zou, Q.C. Zou, X. Xiang, and J.S. Cao. 2007. Effect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers. Plant Cell Tiss. Organ Cult. 88:319-327.   DOI   ScienceOn
21 Manda, G., M.T. Nechifor, and T.M. Neagu. 2009. Reactive oxygen species, cancer and anti-cancer therapies. Curr. Chem. BioI. 3:22-46.   DOI
22 Mishra, K.P., M. Ahmed, and R.P. Hill. 2008. Low-dose radiation effects on human health with implications to radio-protection and cancer radiotherapy. Int. J. Radial. BioI. 84:441-444.   DOI   ScienceOn
23 Oh, C.H., N.S. Kim, K.H. Lee, T.H. Kim, J.B. Bae, S.G. Kim, H. Jeon, J.P. Kim, T.Y. Shin, C.H. Lee, S.I. Jeong, and J. Kwon. 2007. Immuno-regulatory and anti-cancer effect of Acorus gramineus Solander. Kor. J. Oriental. Physiol. Pathol. 21:869-873.
24 Pan, J., J. Wang, Z. Zhou, Y. Yan, W. Zhang, W. Lu, S. Ping, Q. Dai, M. Yuan, B. Feng, X. Hou, Y. Zhang, R. Ma, T. Liu, L. Feng, L. Wang, M. Chen, and M. Lin. 2009. IrrE, a global regulator of extreme radiation resistance in Deinococcus radiodurans, enhances salt tolerance in Escherichia coli and Brassica napus. PLoS ONE 4:e4422.   DOI   ScienceOn
25 Park, J.O., H.S. Cho, M.Y. Park, Y.S. Jo, S.Y. Kang, S.N. Kwack, and B.G. Heo. 2008. Effect of gamma ray irradiation on seed germination, growth and variant induction in Hosta plantaginea and Farfugium japonicum. Flower Res. J. 16:128-133.
26 Parsons, P.A. 2000. Hormesis: an adaptive expectation with emphasis on ionizing radiation. J. Appl. Toxicol. 20:103-112.   DOI   ScienceOn
27 Sancheti, G. and P.K. Goyal. 2007. Evaluation of possible radioprotective action of Rosmarinus officinalis L. in Swiss albino mice. Africa Traditi. Complim. Altern. Med. 4:165-172.
28 Vojtiskova, L., E. Munzarova, O. Votrubova, H. Cizkova, and H. Lipavska. 2006. The influence of nitrogen nutrition on the carbohydrate and nitrogen status of emergent macrophyte Acarus calamus L. Hydrobiologia 563:73-85.   DOI   ScienceOn
29 Yamaguchi, H., A. shimizu, J. Degi, and T. Morishita. 2008. Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum. Breed Sci. 58:331-335.   DOI   ScienceOn
30 Zanoli, P., R. Avallone, and M. Baraldi. 1998. Sedative and hypotherimic effects induced by a-asarone, a main component of Acarus calamus. Phytother. Res. 12: S114-S116.   DOI   ScienceOn
31 Zhang, X.B., P. Liu, Y.S. Yang, and W.R. Chen. 2007. Phytoremediation of urban wastewater by model wetlands with ornamental hydrophytes. J. Environ. Sci. 19:902-909.   DOI   ScienceOn
32 Perez, M.B., N.L. Calderon, and C.A. Croci. 2007. Radiation-induced enhancement of antioxidant activity in extracts of rosemary (Rosmarinus officinalis L.). Food Chem. 1004:585-592.