FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
권호 표지

Radioresistance of Acorus calamus to Gamma Ray Irradiation

  • Lee, Ja-Hyun (Major of Horticulture, Department of Plant Biotechnology, Chonnam National University) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Geung-Joo (Major in Horticultural Science, Division of Bioscience, Mokpo National University) ;
  • Lee, Seong-Gene (Department of Molecular Biotechnology, Bioenergy Research Center, Chonnam National University) ;
  • Kim, Sun-Kook (Jeollanamdo Agricultural Research and Extension Services) ;
  • Han, Tae-Ho (Major of Horticulture, Department of Plant Biotechnology, Chonnam National University)
  • Received : 2011.05.30
  • Accepted : 2011.06.13
  • Published : 2011.06.30
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Abstract

Acorus calamus is an indigenous hydrophyte used as phytoremediation and water purification as well as medicinal herb. Irradiation of gamma ray was performed to investigate the effect on radioresistance of A. calamus on in vitro. Various doses (20-500 Gy) of gamma rays were irradiated to in vitro plantlets. The effect of irradiation was studied on survival rate, shoot formation and growth rate. The survival and multiplication rate was remarkably decreased with the increase of radiation dose. The growth of plantlets was significantly increased in comparison to the control at low doses. The hermetic effect of radiation was appeared in A. calamus. It was significantly decreased or interrupted above 250 Gy. The mean 50% lethal dose ($LD_{50}$) was extremely high as 240 Gy. We conclude that A. calamus had remarkably high radioresistance compared with other plant species.

창포는 한국에 자생하는 수생식물로 약용뿐만 아니라 phytoremediation과 수질정화 식물로도 알려져 있다. 본 연구에서는 창포의 기내 식물체에 다양한 선량(20-500 Gy)의 감마선을 조사하여, 방사선의 효과 및 감수성을 확인하기 위해 2달후 생존율, 신초형성율 및 생장율을 측정하였다. 생존율과 신초형성율은 감마선 선량이 증가할수록 현저하게 감소하였다. 기내에서 저선량을 조사한 식물체의 생장율은 대조구에 비해 오히려 증가하였으나, 250 Gy 이상에서는 생장이 멈추거나 유의하게 감소하였으며, 반치사선량($LD_{50}$)은 240 Gy로 다른 식물종의 $LD_{50}$보다 훨씬 높은 선량이었다. 창포에서 저선량을 조사하였을 때 생장율이 증가하는 방사선호메시스(radiation hormesis) 효과를 확인하였다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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