• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.327-330
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• 2002

A plant growth-promoting substance, FM001, was isolated from the culture broth of Acremonium strictum MJN1. The purification steps included solvent extraction, adsorption chromatography using Diaion HP20, TLC on silica, and HLPC using a C-18 column. The purified FM001 enhanced rice seedling growth by $11.1\%\;and\;34.0\%$ of the dried weight of the shoots and roots, and also radish growth by $26.5\%\;and\;23.7\%$ of the top length and dried weight. FM001 also significantly promoted the growth of red pepper by increasing $32.7\%$ of fruit weight and $11.3\%$ as regards the height. FM001 consisted of C, H, O, N, and S, and its molecular weight was determined to be 537.78 Da. The structure of FM001 resembled brassinosteriods, and it would appear to have great potential as an effective bio-fertilizer.

• Korean Journal of Pharmacognosy
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• v.53 no.3
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• pp.125-132
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• 2022

In this study, we investigated anti-oxidative and anti-inflammatory efficacy, and identified their constituents from Brassica napus L. (Korean name: Yuchae) whole plant. Upon the anti-oxidative activities screening, the ethanol extract exhibited potent DPPH and ABTS⁺ radical scavenging activities. On the anti-inflammation studies using LPS-induced RAW264.7 cells, the extract inhibited the production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) effectively. To identify major constituents of B. napus extract, further purification was performed and led to isolation of two compounds; isorhamnetin 3,7-O-diglucoside(1) and isorhamnetin 3-O-glucoside(2). Quantitative analysis by high pressure liquid chromatography (HPLC) determined the flavonoid 1 as the major constituent. Isolated compounds showed DPPH radical scavenging effects and decreased NO levels without causing cell toxicities. These results indicate that the extract of Yuchae, a rich plant resource in Jeju Island, could be potentially applicable as an anti-oxidative and/or anti-inflammatory ingredients.

• Applied Chemistry for Engineering
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• v.24 no.4
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• pp.423-427
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• 2013

This research was carried out to identify the characteristics of the wastewater from coke oven gas (COG) purification process of the coke plant, and derive optimal operating conditions for the treatment of wastewater. The coke plant wastewater contains highly concentrated $S^{-2}$ and $SCN^-$ that are harmful to microorganisms, and their concentrations were 6.8~11.2 mg/L and 190~320 mg/L, respectively. When the $S^{-2}$ ion concentration was lower than 10 mg/L, $SV_{30}$ of active sludge was 280~ 340 mL and the sludge sedimentation velocity was very fast. But, when the $S^{-2}$ ion concentration was higher than 15 mg/L, $SV_{30}$ of the active sludge was 560~680 mL and the sludge sedimentation velocity was very slow. Also when the $SCN^-$ ion concentration was lower than 300 mg/L, $SV_{30}$ of the active sludge was 245~320 mL and the sludge sedimentation velocity was very fast. But, when the $SCN^-$ ion concentration was higher than 400 mg/L, $SV_{30}$ of the active sludge was 470~ 567 mL and the sludge sedimentation velocity was slow. To treat the wastewater generated by COG purification process of the coke plant effectively and to maintain microorganism activities in good conditions, the ion concentration of $S^{-2}$ and $SCN^-$ should be lower than 15 mg/L and 400 mg/L, respectively.

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.665-674
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• 2015

The final goal of this research is to develop a botanical biofiltration system, which combines green interior, biofiltering, and automatic irrigation, which can purify indoor air pollutants according to indoor space and the size of biofilter. The biofilter used in this experiment was designed as an integral form of water metering pump, water tank, blower, humidifier, and multi-level planting space in order to be more suitable for indoor space utilization. This study was performed to compare indoor air quality between the space adjacent to a botanical biofilter and the space away from the biofilter (control) without generation of artificial indoor air pollutants, and to evaluate plant growth depending on multiple floors within the biofilter. Each concentration of indoor air pollutants such as TVOCs, monoxide, and dioxide in the space treated with the biofilter was lower than that of control. Dracaena sanderiana ‘Vitoria’ and Epipremnum aureum ‘N Joy’ also showed normal growth responses regardless of multiple floors within the biofilter. Hence, it was confirmed that the wall-typed botanical biofilter suitable for indoor plants was effective for indoor air purification.

• Journal of Korea Water Resources Association
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• v.48 no.12
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• pp.1051-1064
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• 2015

Currently, the H purification plant determines the hourly water intake amount based on operator experience and skill. Therefore, inevitably, there are deviations among operators. While meeting time-varying demand and maintaining the proper water level in the clean water reservoir, the methodology for minimizing electricity cost, when dealing with different electricity rate time zones, is a very complicated problem, which is beyond an operator's capability. To solve this problem, a linear programming (LP) model is proposed, which can determine the optimal hourly water intake amount for minimizing the daily electricity cost. It is shown that an inaccurate estimate for the hourly water usage in the demand areas causes the water level constraint to be violated, which is the weak point of the proposed LP method. However, several examples with real-field data show that we can practically and safely solve this problem with safety margins. It is also shown that the safety margin method still works effectively whether the estimate is accurate or not. The operators need not attend the site at all times under the proposed LP method, and we can additionally expect reductions in labor costs.

• Food Science and Biotechnology
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• v.17 no.3
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• pp.573-577
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• 2008

$^1H$-Nuclear magnetic resonance (NMR) spectrometry was applied to the quantitative analysis of coumarins in the roots of Angelica gigas without any chromatographic purification. The experiment was performed by the analysis of each singlet germinal methyl, which was well separated in the range of 1.0-2.0 ppm in the $^1H$-NMR spectrum. The quantity of the compounds was calculated by the ratio of the intensity of each compound to the known amount of internal standard (dimethyl terephthalate). These results were compared with the conventional gas chromatography (GC) method. The contents of decursin and decursinol angelate in A. gigas were determined $1.98{\pm}0.07$, $1.13{\pm}0.08%$ in quantitative $^1H$-NMR method and $2.06{\pm}0.24$, $1.17{\pm}0.24%$ in GC method, respectively. The advantages of quantitative $^1H$-NMR analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curves. Besides, it allows rapid and simple quantification for coumarins with an analysis time for only 10 min without any preprocessing.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.17 no.2
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• pp.31-48
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• 2014

This study is carried out to offer the raw data for conservation and management of tidal flat ecosystem by surveying and analysing the flora distributed in Daesong tidal flat wetland, Ahnsan-si, Gyeonggi-do coast, Korea. The results of surveying the flora were recorded as 186 taxa including 45 families, 121 genera, 170 species, 14 varieties and 2 forms. The halophytes checked around this site were 20 taxa including Atriplex gmelinii, Salicornia europaea, Suaeda glauca, Suaeda japonica, Phragmites communis, Carex scabrifolia and so forth. Polygonum bellardii that species had ecological value was the specific plant by floristic region. The growth locations of halophytes were 11 taxa of upper, 4 taxa of high tide line and 5 taxa of lower. The naturalized plants were 42 taxa including Phytolacca americana, Chenopodium glaucum, Melilotus alba, Veronica persica, Bidens pilosa, Leptochloa fusca and so forth. Because Aster subulatusand Leptochloa fusca grew a upper tidal flat wetland, they had the characteristics of halophytes. The focuses on the management of Daesong wetland were halophytes and naturalized plants. Firstly, to maintain a halophytes communities, we will sow the halophytes seeds and plant the individuals. And In-Situ conservation was applied to Polygonum bellardii habitat. Secondly, to prevent the genesis of naturalized plant, we will don't disturb around the wetland environment. The invasive alien plant, Lactuca scariola, was removed by periodic monitoring and purification activity.

• Korean Journal Plant Pathology
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• v.3 no.2
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• pp.124-130
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• 1987

The optimum conditions for protoplast formation and regeneration from Pyricularia oryzae Cav. were selected as follows. As a basic solution, 0.02M potassium phosphate buffer solution plus 0.6M KCl adjusted to pH 5.2 with 1N HCl was used. A mixture of enzyme combinations with 20mg Cellulase R-l0/ml, 5mg Macerozyme R-l0/ml and l0mg Driselase/ml used as a lytic enzyme showed better lytie effect than any single enzyme treatment for protoplast formation. Two-day-old mycelia of P. oryzae grown in the mixture of three lytie enzyme solution at $30^{\circ}C$ for 3 hr showed best condition for protoplasts formation. For regeneration from the protoplasts of P. oryzae, potato dextrose agar containing 0.02M potassium phosphate plus 0.6M KCl used as a stabilizer was best for regeneration medium.

• Journal of Soil and Groundwater Environment
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• v.20 no.1
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• pp.56-64
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• 2015

Acid mine drainage occurrence is a serious environmental problem by mining industry, it usually contains high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of the greatest concern. An indigenous plant extract was used to produce calcium carbonate from Canavalia ensiformis as effective biomaterial, and its ability to form the calcium carbonate under stable conditions was compared to that of purified urease. X-ray diffraction and scanning electron microscopy were employed to elucidate the mechanism of calcium carbonate formation from the crude plant extracts. The results revealed that urease in the plant extracts catalyzed the hydrolysis of urea in liquid state cultures and decreased heavy metal amounts in the contaminated soil. The heavy metal amounts were decreased in the leachate from the treated mine soil; 31.7% of As, 65.8% of Mn, 50.6% of Zn, 51.6% of Pb, 45.1% of Cr, and 49.7% of Cu, respectively. The procedure described herein is a simple and beneficial method of calcium carbonate biomineralization without cultivation of microorganisms or further purification of crude extracts. This study suggests that crude plant extracts of Canavalia ensiformis have the potential to be used in place of purified forms of the enzyme during remediation of heavy metal contaminated soil.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.327-332
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• 2019

Today's scientists try to remove heavy metals with many new technologies such as phytoremediation. One of the best cutting edge technologies is developing transgenic plants to remove certain heavy metal in soil. I constructed the transformation vector expressing T. goesingense Metal Transport Protein1 gene and TgMTP1: GFP genes. The transgenic plants were selected and confirmed the transformed genes into Arabidopsis thaliana genome. Expression was confirmed in several parts in Arabidopsis cells, tissues and organs. When TgMTP1 overexpressing Arabidopsis thaliana were subjected, transgenic plants showed higher heavy metal tolerance than non-transgenic. For further study I selected the transgenic plant lines with enhanced tolerance against four different heavy metals; Zn, Ni, Co, Cd. The accumulation of these metals in these plants was further analyzed. The TgMTP1 overexpressing Arabidopsis thaliana plant of selected lines are resistant against heavy metals. This plant is characterized by the expression of the MTP1 gene accumulating heavy metal in the vacuole and being simultaneously expressed on the plasma membrane. In conclusion, these plants may be used in plant purification applications, and as a plant with increased tolerance.