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http://dx.doi.org/10.17703/JCCT.2019.5.3.327

Overexpression of the Metal Transport Protein1 gene (MTP1) in Arabidopsis Increased tolerance by expression site  

Kim, Donggiun (Dept. of Life Science, Silla Univ)
Publication Information
The Journal of the Convergence on Culture Technology / v.5, no.3, 2019 , pp. 327-332 More about this Journal
Abstract
Today's scientists try to remove heavy metals with many new technologies such as phytoremediation. One of the best cutting edge technologies is developing transgenic plants to remove certain heavy metal in soil. I constructed the transformation vector expressing T. goesingense Metal Transport Protein1 gene and TgMTP1: GFP genes. The transgenic plants were selected and confirmed the transformed genes into Arabidopsis thaliana genome. Expression was confirmed in several parts in Arabidopsis cells, tissues and organs. When TgMTP1 overexpressing Arabidopsis thaliana were subjected, transgenic plants showed higher heavy metal tolerance than non-transgenic. For further study I selected the transgenic plant lines with enhanced tolerance against four different heavy metals; Zn, Ni, Co, Cd. The accumulation of these metals in these plants was further analyzed. The TgMTP1 overexpressing Arabidopsis thaliana plant of selected lines are resistant against heavy metals. This plant is characterized by the expression of the MTP1 gene accumulating heavy metal in the vacuole and being simultaneously expressed on the plasma membrane. In conclusion, these plants may be used in plant purification applications, and as a plant with increased tolerance.
Keywords
phytoremediation; Overexpression; Arabidopsis; heavy metal tolerance; transgenic plant;
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