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http://dx.doi.org/10.22889/KJP.2022.53.3.125

Anti-oxidative and Anti-inflammatory Constituents from the Extracts of Brassica napus L. Whole Plant  

Jo, Yeon Jeong (R&D Team, Jeju Industry-University Convergence Center)
Hyun, Ju Mi (R&D Team, Jeju Industry-University Convergence Center)
Kang, Ji Mi (R&D Team, Jeju Industry-University Convergence Center)
Kim, Chang Yun (R&D Team, Jeju Industry-University Convergence Center)
Lee, Nam Ho (R&D Team, Jeju Industry-University Convergence Center)
Publication Information
Korean Journal of Pharmacognosy / v.53, no.3, 2022 , pp. 125-132 More about this Journal
Abstract
In this study, we investigated anti-oxidative and anti-inflammatory efficacy, and identified their constituents from Brassica napus L. (Korean name: Yuchae) whole plant. Upon the anti-oxidative activities screening, the ethanol extract exhibited potent DPPH and ABTS+ radical scavenging activities. On the anti-inflammation studies using LPS-induced RAW264.7 cells, the extract inhibited the production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) effectively. To identify major constituents of B. napus extract, further purification was performed and led to isolation of two compounds; isorhamnetin 3,7-O-diglucoside(1) and isorhamnetin 3-O-glucoside(2). Quantitative analysis by high pressure liquid chromatography (HPLC) determined the flavonoid 1 as the major constituent. Isolated compounds showed DPPH radical scavenging effects and decreased NO levels without causing cell toxicities. These results indicate that the extract of Yuchae, a rich plant resource in Jeju Island, could be potentially applicable as an anti-oxidative and/or anti-inflammatory ingredients.
Keywords
Brassica napus L.; Anti-oxidation; Anti-inflammation; Isolation;
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Times Cited By KSCI : 4  (Citation Analysis)
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