• Applied Chemistry for Engineering
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• v.31 no.1
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• pp.1-6
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• 2020

Hydrogen production from biogas has received consistent attention due to the great potential to solve simultaneously the issues of energy demands and environmental problems. Practically, biomethane produced by purification/upgrading of biogas can be a good alternative to the natural gas which is a main reactant for a steam methane reforming process. Judging from the economic and environmental impacts, however, the steam biogas and dry reforming are considered to be more effective routes for hydrogen production because both processes do not require the carbon dioxide elimination step. Herein, we highlight recent studies of hydrogen production via reforming processes using biogas and effective applications for earlier commercialization.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.240-244
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• 2006

We present a fast and simple protocol for purification of mitochondria, mitochondrial DNA, and RNA from small amounts of tomato leaves. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. Mitochondria was confirmed by MitoTracker. The mitochondrial DNA was not contaminated by plastid DNA, was successfully used for PCR. Similarly, the isolated mitochondrial RNA was not contaminated only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for RT-PCR analysis

• KSBB Journal
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• v.15 no.4
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• pp.398-401
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• 2000

Taxiods inclusive paclitaxel were produced isolated and purified from plant cell cultures of Taxus chinensis in large-scale process. their structures were elucidated by spectroscopic analyses. These compounds were exactly identical as those in previous studies from the other biomasses of Taxus chinensis and also other species. Also the concentrations of these compounds were compared with the concentration of the paclitaxel in various batches of plant cell cultures. As paclitaxel concentration increased at the end of cell cultures. the concentrations of the other paclitaxel derivatives decreased. The profile of these taxoids production can provide information for better understanding of structure-activity relationships and biosynthesis Importantly it can be utilized as an useful parameter for the quality control of paclitaxel production.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1337-1345
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• 2014

A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and $6.08{\mu}g/disc$, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.

• Korean journal of applied entomology
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• v.16 no.2 s.31
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• pp.101-104
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• 1977

Potato virus X was purified especially for the preparation of antisera for diagnosis and identification. Potato virus X was isolated Iron infected plants by means of indicator plants and identified in electron microscopy. Isolated PVX was multiplied in tomato plants and purified by a modified procedures. The purity of PVX was 0.59mg/m1. Purified PVX was injected into rabbits once a week for 5 weeks. Antiserum was collected 10 days after the last injection. Produced antiserum was determined 1/1024 titers by means of microprecipitin tests and showed sharp reactions in agar gel-diffusion tests.

• Journal of Microbiology and Biotechnology
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• v.6 no.6
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• Journal of Microbiology and Biotechnology
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• v.22 no.5
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• pp.674-683
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• 2012

A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydroxamate-type siderophore. A bioactive metabolite was isolated from P. aeruginosa SD12 and was characterized as 1-hydroxyphenazine ((1-OH-PHZ) by nuclear magnetic resonance (NMR) spectral analysis. The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani. The stain is also reported to increase the growth and biomass of Plantago ovata. The purified compound, 1-hydroxyphenazine, also showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.

• Korean journal of applied entomology
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• v.16 no.3 s.32
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• pp.145-148
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• 1977

he study was conducted to produce an antiserum of potato virus S for identification and screening of seed-potatoes. Potato virus S was isolated from infected plants and identified by means of indicator plants and electro microscopy. Isolated potato virus S was multiplied in Nicotiana deebneyii and the virus was purified by a modified method that was developed through this study. The purity of potato virus S was 1.18mg/ml. Purified potato virus S was injected into rabbit intravenously once a week for 5 weeks. Antiserum was collected 10 days after the last injection. The produced antiserum was determined to have a titer of, 1/2048 by means of microprecipitin tests.

• Progress in Superconductivity and Cryogenics
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• v.13 no.2
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• pp.21-24
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• 2011

Superconducting high gradient magnetic separation (HGMS) has advantages to treat wastewater because it can generate high magnetic field and achieve rapid purification. In this study superconducting HGMS was applied to remove impurities from the condenser water in thermal power plant. The condenser water contained mainly hematite and maghemite and it was highly magnetized than hematite. In the HGMS tests using a 6-T cryo-cooled Nb-Ti superconducting magnet, the turbidity of the condenser water was effectively reduced up to 99.6% and the result showed better performance than that of the 0.5-T permanent magnet test. The higher magnetic field was applied in the range of 1-6T, the more iron oxides were removed. The effect of magnetic filter configuration on the condenser water treatment was also investigated. Consequently superconducting HGMS system can be applicable to remove iron oxide impurities from condenser water in thermal power plant.

• Korean journal of applied entomology
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• v.17 no.1 s.34
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• pp.29-31
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• 1978

Purely isolated cucumber mosaic virus (CMV) was multiplied in Nicotiana tabacum, Ky-57 and the virus was purified by the modified method that was developed through this study. The concentration of purified CMV was 24.25 mg/ml. The purified virus, mixed with acomplet adjuvant (1: 1) was injected into rabbits intramuscularly. Two injections at 10 day interval was enough to produce a good quality antiserum. The titer of the antiserum was 1/1280 when determined by agar gell-diffusion test. The produced antisera will be used to faciliate the detection of CMV infected vegetables and other crops.