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http://dx.doi.org/10.5352/JLS.2006.16.2.240

Development of a Highly Efficient Isolation Protocol for Mitochondrial DNA and RNA Using Small Scale Plant Tissues  

Kim Kyung-Min (Department of Environmental Horticulture, Sangju National University)
Lim Yong-Suk (Department of Biotechnology, Daegu University of Foreign Studies)
Shin Dong-Ill (Department of Plant Genetic Engineering, Catholic University of Daegu)
Sul Ill-Whan (Department of Biotechnology, Daegu University of Foreign Studies)
Publication Information
Journal of Life Science / v.16, no.2, 2006 , pp. 240-244 More about this Journal
Abstract
We present a fast and simple protocol for purification of mitochondria, mitochondrial DNA, and RNA from small amounts of tomato leaves. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. Mitochondria was confirmed by MitoTracker. The mitochondrial DNA was not contaminated by plastid DNA, was successfully used for PCR. Similarly, the isolated mitochondrial RNA was not contaminated only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for RT-PCR analysis
Keywords
Tomato; mitochondria; mitochondrial DNA; mitochondrial RNA; MitoTracker;
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