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http://dx.doi.org/10.4014/jmb.1405.05008

Purification and Identification of a Novel Antifungal Protein Secreted by Penicillium citrinum from the Southwest Indian Ocean  

Wen, Chao (Key Laboratory of Marine Biogenetic Resources, and Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration)
Guo, Wenbin (Key Laboratory of Marine Biogenetic Resources, and Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration)
Chen, Xinhua (Key Laboratory of Marine Biogenetic Resources, and Collaborative Innovation Center of Deep Sea Biology, Third Institute of Oceanography, State Oceanic Administration)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.10, 2014 , pp. 1337-1345 More about this Journal
Abstract
A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and $6.08{\mu}g/disc$, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.
Keywords
Antifungal protein; plant pathogenic fungi; Southwest Indian Ocean; Penicillium citrinum;
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