• 상하수도학회지
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• 제13권3호
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• pp.101-106
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• 1999

This research aims to compare immobilized catalysts prepared by various methods and determine suitable $TiO_2$ catalyst for water purification. Sol-gel method by Anderson and powder coation method by Tanaka ate famous in the methods to immobilize catalyst. Therefore, the $TiO_2$ catalyst for this research was prepared by sol-gel method and powder coating method. Its structure was tested by X-ray diffractometer (XRD), Scanning electron microseope (SEM). Durability of a catalyst-support couple in an solution was investigated. too. Experimental results were summarized as following; i) Optimum ratio of Ti : $H_2O$ : $H^+$ to obtain stable sol was 1 : 10 : 0.1 and the XRD patterns of $TiO_2$ film immobilized by sol-gel method which were fired at $700^{\circ}C$ showed that the catalyst had an anatase structure. ii) The particle size of $TiO_2$ prepared by sol-gel method was less than $5{\mu}$, but it was observed that coated side was not unifiom. iii) Sol-gel method was very effective to obtain $TiO_2$ catalyst of thin film, but spreadability and durability of a catalyst-support couple in a solution were than $TiO_2$ film immobilized by powder coating method. iv) The particle size of $TiO_2$ immobilized by powder coating method was a little larger than it prepared by sol-gel method, but spreadability and uniformity of $TiO_2$ film and durability of a catalyst-support couple in a solution were better than it immobilized by sol-gel method.

• 대한미생물학회지
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• 제7권1호
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• pp.21-27
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• 1972

생검(生檢)한 나결절(癩結節)에서 간단(簡單)하고 효과적(效果的)인 방법(方法)인 trypsin 처리(處理)와 고속도유침(高速度遠沈)에 의(依)하여 동물접종목적(動物接種目的)에 사용(使用)할수 있는 인나균(人癩菌)의 정제(精製)에 대(對)하여 기술(記述)하였다. 본(本) 방법(方法)의 한 특징(特徵)은 그 정제과정중(精製過程中)에 재래식(在來式)인 나결절조직(癩結節組織)의 마쇄(磨碎)나 유액화조작(乳液化操作)을 전혀 포함(包含)시키지 않은 점(點)이다. 이 방법(方法)에 의(依)하여 정제(精製)된 인나균(人癩菌)을 한국산(韓國産) 다람쥐(Tamias sibiricus asiaticus, Gmelin)의 족저부(足底部) 및 이타조직내(耳朶組織內)에 접종(接種)하였던 바 균접종(菌接種) 8 및 12개월(個月)에 이르러 균증식양상(菌增殖樣狀)이 야기(惹起)됨이 관찰(觀察)되었다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1307-1314
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• 2015

Leukemia inhibitory factor (LIF) is a member of the IL-6 cytokine family, having pleiotropic actions such as maintaining stem cell pluripotency and enabling blastocyst implantation. Because the action of LIF is mediated by a ligand-receptor interaction with the LIF receptor (LIF-R), an antagonist for LIF-R has been developed to inhibit LIF-induced signaling. In this study, we present a novel method for the production and purification of an antagonist to human LIF-R (hLA). His-tagged hLA was expressed in E. coli, and simple purification methods without any endopeptidase cleavage were designed. In addition, we determined the optimal temperature conditions for enhancing the production of soluble hLA. Finally, the bioactivity of His-tagged hLA was examined using STAT3 phosphorylation and receptivity of human endometrial ECC-1 cells. Our strategy provides a rapid and efficient method to produce biologically active recombinant hLA.

• 한국미생물·생명공학회지
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• 제42권1호
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• pp.25-31
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• 2014

본 연구에서는 바이오매스 유래 생리활성물질인 (+)-dihydromyricetin을 효율적으로 정제하기 위하여, 반응액 부피당 표면적(S/V)이 증가된 새로운 개념의 분별침전공정을 도입하였다. 분별침전 24시간에서 반응기 내부 표면적을 증가시키지 않은 경우 순도와 수율은 각각 80.0%와 70.0%인 반면 표면적 증가를 위해 양이온교환수지인 Amberlite 200을 첨가한 경우 순도와 수율은 각각 90.2%와 90.9%로 가장 효과적인 표면적증가물질임을 알 수 있었다. 특히 Amberlite 200의 경우 상대적으로 짧은 침전시간(16시간)에 높은 수율(>90%)로 (+)-dihydromyricetin을 얻을 수 있어 침전에 소요되는 시간을 효과적으로 단축시킬 수 있었다. 동일한 침전시간에서는 표면적증가물질을 첨가하지 않은 경우에 비해 표면적증가물질을 첨가한 경우 침전물의 입자 크기가 감소함을 알 수 있었다. 이러한 연구결과는 기존의 분별침전 방법에 의한 (+)-dihydromyricetin 정제공정의 문제점을 개선함으로써(+)-dihydromyricetin의 대량생산에 매우 유용하게 활용될 수 있을 것으로 판단된다.

• Korean Chemical Engineering Research
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• 제59권1호
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• pp.106-111
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• 2021

본 연구에서는 식물세포 Taxus chinensis로부터 항암물질 파클리탁셀을 효율적으로 정제하기 위하여, 초음파 기반 마이셀 추출 공정을 개발하였다. 전통적 마이셀 공정(대조군)에서의 많은 추출 단계 및 긴 상 분리 시간 문제를 획기적으로 개선하였다. 초음파 파워 180 W, 초음파 조사 1.5 시간에서 가장 높은 파클리탁셀 수율(~96%, 2회 추출)을 얻었으며, 이는 대조군의 수율에 비해 24.7% 증가하였다. 또한 분배 계수(K)는 초음파 파워 180 W, 초음파 조사 1.5 시간에서 최대치(24.0)를 보였다. 파클리탁셀 순도에는 큰 차이가 없었으며, 초기 파클리탁셀의 순도(6.81%)가 정제 후 22%까지 증가하였다. 역 추출(back extraction)의 상 분리 시간은 대조군 대비 각각 40.7-56.2%(초음파 파워 80 W), 46.3-67.6%( 초음파 파워 180 W), 51.9-67.6%(초음파 파워 250 W) 감소하였다. 초음파 파워(80-250 W)와 초음파 조사 시간(0.5-2.5 시간)이 증가할수록 상 분리 시간이 감소하였다.

• 반도체디스플레이기술학회지
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• 제20권4호
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• pp.89-95
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• 2021

As the influence of fine dust on society spreads gradually, the public's interest in indoor air is increasingly rising. Air-purifying plants are drawing keen attention due to their natural purifying function enabled by plant physiology. However, as their fine dust reduction mechanism is limited to adsorption only, vegetation bio-filters that optimize purification effects through integration with air-conditioning systems is rising as an alternative. In accordance with the relevant standard test methods, this study looked into the fine dust reduction assessment method by air-conditioning airflow volume that can be used for the industrial spread of vegetation bio-filters. In the case of PM₁₀ at 300 ㎍/m³, it was in the order of EG-B(3,500CMH, 29 min.) < EG-A (2,500CMH, 37 min.) < CG(0CMH, 64 min.) for reaching the maintenance level (100 ㎍/m³) of publicly used facilities. For reaching the WHO Guideline(50 ㎍/m³) requirement, it was in the order of EG-B (51 min.) < EG-A (160 min.) < CG (170 min.). In the case of PM_2.5, it was in the order of EG-B (26 min.) < EG-A (33 min.) < CG (57 min.) for reaching the maintenance level (50 ㎍/m³) of publicly used facilities. It was in the order of EG-B (48 min) < EG-A (140 min) < CG (158 min) for reaching the WHO Guideline (25 ㎍/m³) requirement. The findings from the analysis showed that fine dust can be reduced most efficiently when the system is operated at 3,500CMH level. The limitation of this study is that due to the absence of a way of assessing the stress of plants in vegetation bio-filters, generating optimal air-conditioning air flow of the relevant system and economics analysis against the existing facility-type air purification system have been clarified, which should be explored further though follow-up studies.

• 약학회지
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• 제30권1호
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• pp.31-41
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• 1986

Sarcolemmal membrane fraction from canine ventricle was isolated from the discarded pellet after the first homogenization in the isolation procedure of sarcoplasmic reticulum (Method 1) and the protein yield, purity, and sidedness of this preparation were compared to those of sarcolemmal fraction prepared by method of Lee et al. (Method 2) and a slight modification of original protocol of Jones et al. (Method 3). Method 1 differed from Method 2 essentially only in that vigorous homogenization was carried out by omnimixer and homogenization medium containing 30mM Tris-maleate was used in the first step. The sarcolemmal fraction was enriched from 45 to 50 and 29-fold in [$^3H$] ouabain, [$^3H$] DHA, [$^3H$] QNB binding and $Na^+$, $K^+$-ATPase activity, respectively, compared to homogenate. Total $Na^+$, $K^+$-ATPase activity of highly sarcolemma enriched fraction was 144.6$\pm$16.4$\mu\textrm{mol}$ Pi/mg protein/hr, which was about 85%, of total ATPase activity, and the yield of the preparation was 15.7 mg protein per 100g of starting ventricular tissue. The sarcolemmal preparation supported $^{45}Ca^{2+}$-uptake in the presence of ATP but this uptake was not dependent on oxalate. Sarcolemmal $Na^+$, $K^+$-ATPase activity and detectable [$^3H$] ouabain binding were increased about 32% and 35%, respectively, by pretreatment of sarcolemmal fraction with optimal concentration of sodium dodecylsulfate (0.3-0.4mg/mg protein), suggesting that this preparation contained about 24% of sealed rightside-out vesicles, 26% of sealed inside-out vesicles, and 5001o of freely permeable (leaky) form. This procedure showed the highest protein yield and leaky population, compared to Method 2 and 3. On the other hand, sarcolemmal fraction prepared by Method 2 and 3 showed low value in protein yield but comtained high population of inside-out (46%) and rightside-out (49%) vesicles, respectively, compared to present procedure (Method 1). The results indicate that vigorous homogenization decreases the population of sealed sarcolemmal vesicles but increases the sarcolemmal protein yield per gram tissue and that this procedure is available for further purification of sarcolemmal fraction and for the receptor binding study of sarcolemma.

• Food Science and Biotechnology
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• 제18권2호
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• pp.565-569
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• 2009

This study was conducted to evaluate the effects of different preparation methods on the recovery and quantification of ginsenosides in raw Korean ginseng (Panax ginseng C.A. Meyer). Eight major ginsenosides ($Rb_1$, $Rb_2$, $Rb_3$, Rc, Rd, Re, Rf, and $Rg_1$) were analyzed by high performance liquid chromatography (HPLC), after which the recovery and repeatability of the extraction of those ginsenosides using 3 different preparation methods were compared [A. direct extraction (DE) method, hot MeOH extraction/evaporation/direct dissolution; B. solid phase extraction (SPE) method, hot MeOH extraction/evaporation/dissolution/$C_{18}$ cartridge adsorption/MeOH elution; C. liquid-liquid extraction (LLE) method, hot MeOH extraction/evaporation/dissolution/n-BuOH fractionation]. Use of the DE method resulted in a significantly higher recovery of total ginsenosides than other methods and a relatively clear peak resolution. Use of the SPE and LLE methods resulted in clearer peak resolution, but lower ginsenoside recovery than the DE method. The LLE method showed the lowest ginsenoside recovery and repeatability among the 3 methods. Given that the DE method employed only extraction, evaporation, and a dissolution step (avoiding complicate and time consuming purification), this technique may be an effective method for the preparation and quantification of ginsenosides from raw Korean ginseng.

• 한국환경보건학회지
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• 제17권2호
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• pp.35-40
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• 1991

This study was performed to investigate the contamination degree of effluent from the sewage purification system and septic tank. The 711 samples, were collected from the large size tank located in Seoul from Mar. to June 1991. The results were as follows 1. The pH values of effluent were largely neutral of 6.96 in sewage and 7.43 in septic tank. 2. The average concentration of BOD was 48.18 mg/l in sewage and 127.0 rng/l in septic tank. 3. The average concentration of SS was 40.8mg/l in sewage and 90.5rng/l in septic tank. 4. In the analysis of nutrient salts, the average concentration of NH$_{3}$-N was 31.62mg/l in sewage and 88.79 mg/l in septic tank. 5. In the analysis of correlation among items, BOD, SS, NH$_{3}$-N and PO$_{4}$-P were higly correlated beween items. 6. As a results of above analysis, it is considered as desirable evaluation method of effluent not through the only item but through the integrated items.

• 한국임상수의학회지
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• 제11권1호
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• pp.389-392
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• 1994

To purify transforming growth factor type beta(TGF-$\beta$) in canine platelets, Sephadex G-75 gel filtration and semipreparative HPLC were carried out. The column of $2.0 {\times}120cm$ was used for gel filtration and one inch semipreparative column filled with SP-Toyopeal for HPLC. Electrophoresis and bioassay using African green monkey kidney cell were used for identification of TGF-$\beta$ Crude TGF-$\beta$ of 2.75mg was extracted from 5.2g of the platelets by the treatment of acid/ethanol. In gel filtration of crude TGF-$\beta$, 4 peaks were observed at the detection of spectrophotometer at 280nm. Electrophoresis and bioassay identified the 3rd peak TGF-$\beta$. Linear gradient elution from 0 to 3M NaCl in sornipreparative HPLC showed TGF-$\beta$ at 1.5M NaCl. Gel filtration was less expensive and useful method for the purification of TGF-$\beta$.