• 제목/요약/키워드: Pure sciences

검색결과 782건 처리시간 0.032초

가청음에 의한 독가시치의 청각 능력 2. 청각 임계비 (The Hearing Ability of the Dusky spinefoot Siganus fuscescens(Houttuyn)to Audible Sound 2. The Auditory Critical Ratio)

  • 이창헌;문종욱;서두옥
    • 수산해양교육연구
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    • 제12권2호
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    • pp.191-198
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    • 2000
  • 이 연구는 제주도 연안에 분포되어 있는 정치망에서 음향 집어기에 의한 어류의 유집에 대한 기초 자료를 제공할 목적으로, 정치망의 주요 어획 대상 어류인 독가시치를 선택하여, 가청 수중음과 전기 자극을 이용하여 음향 조건학습시켰으며, 주파수와 음압을 변화시켜가면서 심전도를 도출하여 심박 간격의 변화로부터 청각 능력을 알아내는 방법을 이용하여, 독가시치의 청각 문턱치 및 청각 임계비를 측정 조사 한 결과 75, 79, 83의 3단계 백색잡음을 방성하였을 때 독가시치의 청각 문턱치는 백색잡음이 없을 때보다 높게 나타나 마스킹 효과가 나타났고, 측정주파수 200Hz, 300Hz에서 보다 뚜렷하게 나타났다. 독가시치의 청각 임계비는 측정주파수 80, 100, 200, 300, 500, 800Hz에서 대략 28, 23, 31, 34, 40, 52dB이고 마스킹 현상은 음압이 약 70dB 이상의 잡음레벨에서 나타나기 시작하였고 측정주파수 100 - 200Hz에서 신호음을 인식하기 위해서는 음압이 대략 98dB이상, 배경잡음레벨보다 약 23dB이상의 높은 음압이 요구되었다.

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Identification of Iranian Vectors of Malaria by Analysis of Cuticular Hydrocarbons

  • Rasoolian, Mohammad;Nikbakhtzadeh, Mahmood Reza
    • Animal cells and systems
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    • 제13권3호
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    • pp.331-337
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    • 2009
  • Twenty-eight Anopheles species has been so-far identified in Iran, while only 8 species was proved as malaria vector. In this study, we principally examined the cuticular hydrocarbon (CHC) potency in identification of Iranian vectors of malaria and then differentiation of vector and non-vector species of Anopheles. Seven species of malaria vectors and the non-vector species, Anopheles claviger were collected throughout Iran. Female extracts were made out of every five conspecific specimens by surface immersion in pure n-hexane. Each sample was injected into a FID-GC instrument along with the known concentrations of standards. CHC profiles of the eight Anopheles species indicated no qualitative difference. The average mass of each eluted CHC were compared using Repeated ANOVA and Mann-Whitney tests. Results confirmed a significant difference in mass of each single CHC at a specific retention time (RT). Statistical comparison of CHC mass in An. sacharovi, An. stephensi, An. culicifacies and An. fluviatilis at RT 39.6 indicated significant differences (P<0.05) among these species. Analysis of CHC mass of An. dthali, An. superpictus & An. sacharovi at RT 28.5, An. stephensi & An. sacharovi at RT 30.7 and An. sacharovi & An. claviger at RT 30.6 similarly indicated significant differences (P<0.05). An. sacharovi could be distinguished from other species, which showed only trace, by integratable peaks at retention times of 29.7, 31 and 32.6. Similarly, An. claviger could be distinguished from the other species with a trace peak at RT 30.6. In order to separate An. stephensi from the five other species, the integratable peak at RT 30.7 was used. An. dthali could be identified at RT 26.2 by an integratable peak v.s. the trace peaks of other species. An. superpictus had indicator peaks at RTs 27.4 & 28.5 v.s. trace peaks of other species. An. maculipennis with its trace peak at RT 39.6 could be easily differentiated from An. fluviatilis & An. culicifacies. This study proved that all of the examined species of Anopheles could be well identified based on their quantitative differences in CHCs, except for An. fluviatilis & An. culicifacies for which no CHC indicator peak was detected.

디지털 방식 FM 합성 신호 발생기의 구현 (Implementation of a digital FM composite signal generator)

  • 정도영;김대용;유영갑
    • 한국통신학회논문지
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    • 제23권5호
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    • pp.1349-1359
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    • 1998
  • 본 논문에서는 디지털 FM 스테레오 합성 신호 발생기(FM stereo composite signal generator)의 구현 결과를 제시하였다. 직접 디지털 주파수 합성기(DDFS)를 응용하여 단일 칩으로 디지털화 하였으며, $1.0\mu\textrm{m}$ CMOS 게이트­어레이 기술로 구현하였다. 설계 결과는 시뮬레이션을 통해 신호 발생 과정을 검증하였고, 디지털 칩을 실장한 평가용 인쇄회로기판을 제작하여 신호 발생 값을 비교 분석하였다. 측정 결과 디지털-아날로그 변환기의 비트 수가 12비트일 때 신호 대 잡음비가 74dB가 측정되었으며, 이는 아날로그 회로보다 14dB 더 우수한 것이다. 범용 스테레오 입출력으로 16비트 디지털-아날로그 변환기를 사용할 경우 아날로그 방식보다 훨씬 우수한 스펙트럼 순수도를 얻을 수 있을 것으로 기대한다. 디지털 FM 스테레오 합성 신호 발생기는 신호 대 잡음비, 정확도, 튜닝 안정성,그리고 집적도측면에서 기존의 아날로그회로보다 우수한 특성을 보인다.

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Cloning, Sequencing, and Expression of the Gene Encoding a Multidomain Endo-$\beta$-1,4-Xylanase from Paenibacillus curdlanolyticus B-6, and Characterization of the Recombinant Enzyme

  • Waeonukul, Rattiya;Pason, Patthra;Kyu, Khin Lay;Sakka, Kazuo;Kosug, Akihiko;Mori, Yutaka;Ratanakhanokchai, Khanok
    • Journal of Microbiology and Biotechnology
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    • 제19권3호
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    • pp.277-285
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    • 2009
  • The nucleotide sequence of the Paenibacillus curdlanolyticus B-6 xyn10A gene, encoding a xylanase Xyn10A, consists of 3,828 nucleotides encoding a protein of 1,276 amino acids with a predicted molecular mass of 142,726 Da. Sequence analysis indicated that Xyn10A is a multidomain enzyme comprising nine domains in the following order: three family 22 carbohydrate-binding modules (CBMs), a family 10 catalytic domain of glycosyl hydrolases (xylanase), a family 9 CBM, a glycine-rich region, and three surface layer homology (SLH) domains. Xyn10A was purified from a recombinant Escherichia coli by a single step of affinity purification on cellulose. It could effectively hydrolyze agricultural wastes and pure insoluble xylans, especially low substituted insoluble xylan. The hydrolysis products were a series of short-chain xylooligosaccharides, indicating that the purified enzyme was an endo-$\beta$-1,4-xylanase. Xyn10A bound to various insoluble polysaccharides including Avicel, $\alpha$-cellulose, insoluble birchwood and oat spelt xylans, chitin, and starches, and the cell wall fragments of P. curdlanolyticus B-6, indicating that both the CBM and the SLH domains are fully functioning in the Xyn10A. Removal of the CBMs from Xyn10A strongly reduced the ability of plant cell wall hydrolysis. These results suggested that the CBMs of Xyn10A play an important role in the hydrolysis of plant cell walls.

저 레이놀즈 수 영역에서 날갯짓 비행체 공력 모델의 실험적 검증 (Experimental Validation of Ornithopter Aerodynamic Model in Low Reynolds Number Regime)

  • 이준성;김대관;한재흥
    • 한국항공우주학회지
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    • 제38권7호
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    • pp.647-654
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    • 2010
  • 본 연구에서는 날갯짓 비행체 날개의 유체-구조 연계를 고려한 설계나 날갯짓 비행체의 비행 동역학 및 제어 시뮬레이션에 적용 가능한 효율적인 공력모델을 제안하고, 풍동 실험을 통해 공력모델의 특성을 검증하고자 한다. 날갯짓 비행체는 저 레이놀즈 수 영역의 비정상 유동장의 지배를 받기 때문에, 이 영역에서 날개 운동에 따른 공력을 효과적으로 측정할 수 있도록 풍동실험장치를 설계 및 개발하였다. 본 연구의 실험장치 특성상 힘을 측정하는 2축-로드셀은 비관성계에 있기 때문에, 순수한 날개의 공력을 측정하기 위해서는 관성력을 보정해주어야 하며, 이에 대한 방법론을 수립하였다. 최종적으로 유동속도, 날개의 운동 주파수 및 고정 받음각에 따라 날개에 작용하는 양력 및 항력의 평균값 및 평균 제곱근 값을 비교함으로서 실험결과와 공력모델의 특성을 비교 검증하였다.

제주 연안에서 어획된 황놀래기의 청각 능력 (Hearing Ability of Bambooleaf wrasse Pseudolabrus japonicus caught in the coast of Jeju)

  • 최찬문;박용석;이창헌
    • 수산해양교육연구
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    • 제25권6호
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    • pp.1381-1388
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    • 2013
  • In order to improve the availability of underwater sound by the fundamental data on the hearing ability, the auditory thresholds for the bambooleaf wrasse pseudolabrus japonicus were determined at 80Hz, 100Hz, 200Hz, 300Hz, 500Hz and 800Hz by heartbeat conditioning method using pure tones coupled with a delayed electric shock. The audible range of the bambooleaf wrasse extended from 80Hz to 800Hz with the best sensitivity around 100Hz and 200Hz. In addition, the auditory thresholds over 300Hz increased rapidly. The mean auditory thresholds of the bambooleaf wrasse at the test frequencies, 80Hz, 100Hz, 200Hz, 300Hz, 500Hz and 800Hz were 100dB, 95.1dB, 94.8dB, 109dB, 121dB and 125dB, respectively. Auditory critical ratios for the bambooleaf wrasse were measured using masking stimuli with the spectrum level range of about 70, 74, 78dB (0dB re $1{\mu}Pa/\sqrt{Hz}$). According to white noise level, the auditory thresholds increased as compared with thresholds in a quiet background noise. The Auditory masking by the white noise spectrum level was stared over about 60dB within 80~300Hz. Critical ratios to be measured at frequencies from 80Hz to 300Hz were minimum 33dB and maximum 39dB.

고흥만 인공습지의 토양유기탄소와 이산화탄소 변동 관측 (Observations of Variations in Soil Organic Carbon and Carbon Dioxide in the Constructed Wetland at Goheung Bay)

  • 강동환;김성수;권병혁;김일규
    • 수산해양교육연구
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    • 제20권1호
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    • pp.58-67
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    • 2008
  • Seasonal variations in carbon dioxide in the air and soil organic carbon in the sediments were monitored at the constructed wetland formed by reclamation work at Goheung Bay. Sediment sampling in the constructed wetland and carbon dioxide measurement in the air were conducted on June 16 and August 23, 2007. Sediments in the constructed wetland were sampled at 11 different points (June 16) and 14 points (August 23), while carbon dioxide in the air was measured at 13 points (June 16) and 15 points (August 23). Water content and organic carbon in the sampled sediments were analyzed in the laboratory. Water content of the sediments was higher than that of general soil, and the variation between June and August was not evident. The amounts of organic carbons in the sediments sampled on August 23 were higher than those sampled on June 16. Also, there was more organic carbon in the sediments sampled at the field of reeds than in the pure wetland area. Daily maximum variation in carbon dioxide in the air was higher on June 16, but the amount of carbon dioxide in the air was greater on August 23. The results of the study suggest that organic carbon in the sediments and carbon dioxide in the air were greater in summer (August 23) than in spring season (June 16) in the constructed wetland at Goheung Bay.

Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb3 in an isomeric mixture

  • Cui, Chang-Hao;Fu, Yaoyao;Jeon, Byeong-Min;Kim, Sun-Chang;Im, Wan-Taek
    • Journal of Ginseng Research
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    • 제44권6호
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    • pp.784-789
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    • 2020
  • Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb3 from ginseng extracts is limited by the co-existence of its isomer Rb2. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb3 from a mixture of isomers. Methods: To isolate Rb3 from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb2 into ginsenoside Rd. Ginsenoside Rb3 was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb3 was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb3 can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures.

전기모터를 사용한 소형 무인헬리콥터에 활용될 항법장치용 자장계의 보상 (Compensation of Magnetometer in the Navigation System for Unmanned Helicopter using an Electric Motor)

  • 이길호;조성범;김정성;최기영;기창돈;송용규;구훤준
    • 한국항공우주학회지
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    • 제40권11호
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    • pp.997-1003
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    • 2012
  • GNSS와 ARS는 저가의 무인 항공기를 위한 보편적인 센서로, 오차가 크며 방위각을 독립적으로 알아낼 수 없다는 단점이 있다. 또한 GNSS/INS는 초기정렬 과정과 직선비행 중에 IMU의 오차를 충분히 보상하지 못한다. 자장계는 방위각을 얻는데 효과적으로 활용되는데, 소형의 헬리콥터에 사용되는 전기모터는 정확한 방위각 산출을 방해한다. 이 논문에서는 자장계를 보상하는 방법을 제안하였으며, 비행시험으로 검증하였다. 또한 시뮬레이션 결과는 GNSS/INS에 추가된 자장계가 관측성과 정확도를 높이는 데 효과적임을 보여준다.

Retrospective clinical study of multiple keratocystic odontogenic tumors in non-syndromic patients

  • Hwang, Dae-Seok;Kim, Yun-Ho;Kim, Uk-Kyu;Ryu, Mi-Heon;Kim, Gyoo-Cheon
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제44권3호
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    • pp.107-111
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    • 2018
  • Objectives: A keratocystic odontogenic tumor (KOT) is a type of odontogenic tumor that mainly occurs in the posterior mandible. Most KOTs appear as solitary lesions; however, they sometimes occur as multiple cysts. This study analyzed the clinical features of multiple KOTs. Materials and Methods: The participants were diagnosed with KOT by biopsy with multiple surgical sites, and were patients at the Pusan National University Hospital and the Pusan National University Dental Hospital from January 1, 2005 to March 31, 2016. Charts, records, images and other findings were reviewed. Results: A total of 31 operations were conducted in 17 patients. The mean patient age was $28.4{\pm}20.1years$. Multiple KOTs were found to occur at a young age (P<0.01). The predominant sites were in the posterior mandible (28.6%). Most cases of multiple lesions appeared in both the upper and lower jaw, and 40.3% of lesions were associated with unerupted and impacted teeth. The overall recurrence rate measured by operation site was 10.4% (8/77 sites). No patients were associated with nevoid basal cell carcinoma syndrome. Conclusion: The pure recurrence rate was lower than estimated, but there was a higher possibility of secondary lesions regardless of the previous operation site; therefore, long-term follow-up is necessary.