• Title/Summary/Keyword: Pure sciences

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The Hearing Ability of the Dusky spinefoot Siganus fuscescens(Houttuyn)to Audible Sound 2. The Auditory Critical Ratio (가청음에 의한 독가시치의 청각 능력 2. 청각 임계비)

  • Lee, Chang-Heon;Moon, Jong-Wook;Seo, Du-Ok
    • Journal of Fisheries and Marine Sciences Education
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    • v.12 no.2
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    • pp.191-198
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    • 2000
  • An experiment was carried out to obtain the fundamental data on the auditory thresholds of fishes for catching method using audible frequency sound, the auditory thresholds of dusky spinefoot Siganus fuscescens(Houttuyn) were measured in the presence of masking noise in the spectrum level range of 74 - 83dB re $1{\mu}Pa/{\sqrt{Hz}}$ by heartbeat conditioning technique using pure tones coupled with a delayed electric shock. The auditory critical ratios were about 23 - 34dB at measurement frequency range. The ratio increased almost linearly with increasing frequency from 200 to 500Hz. The noise spectrum level at the start of masking was about 61 - 73dB within the measurement frequency range. This suggests that hearing of dusky spinefoot is masked in the natural environment with the noise spectrum level above 70dB. The sound pressure level of which the signal sound of 100Hz is recognized by dusky spinefoot under the white noise of 70dB is above 98dB and the critical ratio of them is above 23dB.

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Identification of Iranian Vectors of Malaria by Analysis of Cuticular Hydrocarbons

  • Rasoolian, Mohammad;Nikbakhtzadeh, Mahmood Reza
    • Animal cells and systems
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    • v.13 no.3
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    • pp.331-337
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    • 2009
  • Twenty-eight Anopheles species has been so-far identified in Iran, while only 8 species was proved as malaria vector. In this study, we principally examined the cuticular hydrocarbon (CHC) potency in identification of Iranian vectors of malaria and then differentiation of vector and non-vector species of Anopheles. Seven species of malaria vectors and the non-vector species, Anopheles claviger were collected throughout Iran. Female extracts were made out of every five conspecific specimens by surface immersion in pure n-hexane. Each sample was injected into a FID-GC instrument along with the known concentrations of standards. CHC profiles of the eight Anopheles species indicated no qualitative difference. The average mass of each eluted CHC were compared using Repeated ANOVA and Mann-Whitney tests. Results confirmed a significant difference in mass of each single CHC at a specific retention time (RT). Statistical comparison of CHC mass in An. sacharovi, An. stephensi, An. culicifacies and An. fluviatilis at RT 39.6 indicated significant differences (P<0.05) among these species. Analysis of CHC mass of An. dthali, An. superpictus & An. sacharovi at RT 28.5, An. stephensi & An. sacharovi at RT 30.7 and An. sacharovi & An. claviger at RT 30.6 similarly indicated significant differences (P<0.05). An. sacharovi could be distinguished from other species, which showed only trace, by integratable peaks at retention times of 29.7, 31 and 32.6. Similarly, An. claviger could be distinguished from the other species with a trace peak at RT 30.6. In order to separate An. stephensi from the five other species, the integratable peak at RT 30.7 was used. An. dthali could be identified at RT 26.2 by an integratable peak v.s. the trace peaks of other species. An. superpictus had indicator peaks at RTs 27.4 & 28.5 v.s. trace peaks of other species. An. maculipennis with its trace peak at RT 39.6 could be easily differentiated from An. fluviatilis & An. culicifacies. This study proved that all of the examined species of Anopheles could be well identified based on their quantitative differences in CHCs, except for An. fluviatilis & An. culicifacies for which no CHC indicator peak was detected.

Implementation of a digital FM composite signal generator (디지털 방식 FM 합성 신호 발생기의 구현)

  • 정도영;김대용;유영갑
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.23 no.5
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    • pp.1349-1359
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    • 1998
  • In this paper, presented is the result of a digital implementation of a FM stereo composite signal generator. The chip utilizing DDFS(Direct Digital Frequency Synthesizer architecture is implemented using $1.0\mu\textrm{m}$ CMOS gate-array technology thereby replacing analog componentry. To verify the process of generating composite signals a conventional logic simulation method was used. The processed chip was mounted on an evaluation PCB to test and analyze to signals. According to the measurement result obtained by using a 12-bit DAC, the digital FM composite signal generator produces a 74DB spectrally pure signal over its entire tuning range, which is superior to that of analog counterpart by 14dB in it spectral reponse. And further enhancements of the spectral response is expected to be achieved by using a high resolution digital to analog converter, such as a 16-bit DAC. The resulting signals is superior to the signal of the analoy circuitry typically used, in major characteristics such as S/N ratios, accuracy, tuning stability, and signal seperation.

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Cloning, Sequencing, and Expression of the Gene Encoding a Multidomain Endo-$\beta$-1,4-Xylanase from Paenibacillus curdlanolyticus B-6, and Characterization of the Recombinant Enzyme

  • Waeonukul, Rattiya;Pason, Patthra;Kyu, Khin Lay;Sakka, Kazuo;Kosug, Akihiko;Mori, Yutaka;Ratanakhanokchai, Khanok
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.277-285
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    • 2009
  • The nucleotide sequence of the Paenibacillus curdlanolyticus B-6 xyn10A gene, encoding a xylanase Xyn10A, consists of 3,828 nucleotides encoding a protein of 1,276 amino acids with a predicted molecular mass of 142,726 Da. Sequence analysis indicated that Xyn10A is a multidomain enzyme comprising nine domains in the following order: three family 22 carbohydrate-binding modules (CBMs), a family 10 catalytic domain of glycosyl hydrolases (xylanase), a family 9 CBM, a glycine-rich region, and three surface layer homology (SLH) domains. Xyn10A was purified from a recombinant Escherichia coli by a single step of affinity purification on cellulose. It could effectively hydrolyze agricultural wastes and pure insoluble xylans, especially low substituted insoluble xylan. The hydrolysis products were a series of short-chain xylooligosaccharides, indicating that the purified enzyme was an endo-$\beta$-1,4-xylanase. Xyn10A bound to various insoluble polysaccharides including Avicel, $\alpha$-cellulose, insoluble birchwood and oat spelt xylans, chitin, and starches, and the cell wall fragments of P. curdlanolyticus B-6, indicating that both the CBM and the SLH domains are fully functioning in the Xyn10A. Removal of the CBMs from Xyn10A strongly reduced the ability of plant cell wall hydrolysis. These results suggested that the CBMs of Xyn10A play an important role in the hydrolysis of plant cell walls.

Experimental Validation of Ornithopter Aerodynamic Model in Low Reynolds Number Regime (저 레이놀즈 수 영역에서 날갯짓 비행체 공력 모델의 실험적 검증)

  • Lee, Jun-Seong;Kim, Dae-Kwan;Han, Jae-Hung
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.38 no.7
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    • pp.647-654
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    • 2010
  • In this study, an efficient ornithopter aerodynamic model, which is applicable to ornithopter wing design considering fluid-structure interaction or ornithopter flight dynamics and control simulation, was proposed and experimentally validated through the wind tunnel experiments. Due to the ornithopter aerodynamics governed by unsteady low Reynolds number flow, an experimental device was specially designed and developed. A part of the experimental device, 2-axis loadcell, was situated in the non-inertial frame; the dynamic calibration method was established to compensate the inertial load for pure aerodynamic load measurements. The characteristics of proposed aerodynamic model were compared with the experimental data in terms of mean and root-mean-square values of lift and drag coefficients with respect to the flow speed, flapping frequency, and fixed angle of attack.

Hearing Ability of Bambooleaf wrasse Pseudolabrus japonicus caught in the coast of Jeju (제주 연안에서 어획된 황놀래기의 청각 능력)

  • Choi, Chan-Moon;Park, Yong-Seok;Lee, Chang-Heon
    • Journal of Fisheries and Marine Sciences Education
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    • v.25 no.6
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    • pp.1381-1388
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    • 2013
  • In order to improve the availability of underwater sound by the fundamental data on the hearing ability, the auditory thresholds for the bambooleaf wrasse pseudolabrus japonicus were determined at 80Hz, 100Hz, 200Hz, 300Hz, 500Hz and 800Hz by heartbeat conditioning method using pure tones coupled with a delayed electric shock. The audible range of the bambooleaf wrasse extended from 80Hz to 800Hz with the best sensitivity around 100Hz and 200Hz. In addition, the auditory thresholds over 300Hz increased rapidly. The mean auditory thresholds of the bambooleaf wrasse at the test frequencies, 80Hz, 100Hz, 200Hz, 300Hz, 500Hz and 800Hz were 100dB, 95.1dB, 94.8dB, 109dB, 121dB and 125dB, respectively. Auditory critical ratios for the bambooleaf wrasse were measured using masking stimuli with the spectrum level range of about 70, 74, 78dB (0dB re $1{\mu}Pa/\sqrt{Hz}$). According to white noise level, the auditory thresholds increased as compared with thresholds in a quiet background noise. The Auditory masking by the white noise spectrum level was stared over about 60dB within 80~300Hz. Critical ratios to be measured at frequencies from 80Hz to 300Hz were minimum 33dB and maximum 39dB.

Observations of Variations in Soil Organic Carbon and Carbon Dioxide in the Constructed Wetland at Goheung Bay (고흥만 인공습지의 토양유기탄소와 이산화탄소 변동 관측)

  • Kang, Dong-Hwan;Kim, Sung-Soo;Kwon, Byung-Hyuk;Kim, Il-Kyu
    • Journal of Fisheries and Marine Sciences Education
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    • v.20 no.1
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    • pp.58-67
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    • 2008
  • Seasonal variations in carbon dioxide in the air and soil organic carbon in the sediments were monitored at the constructed wetland formed by reclamation work at Goheung Bay. Sediment sampling in the constructed wetland and carbon dioxide measurement in the air were conducted on June 16 and August 23, 2007. Sediments in the constructed wetland were sampled at 11 different points (June 16) and 14 points (August 23), while carbon dioxide in the air was measured at 13 points (June 16) and 15 points (August 23). Water content and organic carbon in the sampled sediments were analyzed in the laboratory. Water content of the sediments was higher than that of general soil, and the variation between June and August was not evident. The amounts of organic carbons in the sediments sampled on August 23 were higher than those sampled on June 16. Also, there was more organic carbon in the sediments sampled at the field of reeds than in the pure wetland area. Daily maximum variation in carbon dioxide in the air was higher on June 16, but the amount of carbon dioxide in the air was greater on August 23. The results of the study suggest that organic carbon in the sediments and carbon dioxide in the air were greater in summer (August 23) than in spring season (June 16) in the constructed wetland at Goheung Bay.

Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb3 in an isomeric mixture

  • Cui, Chang-Hao;Fu, Yaoyao;Jeon, Byeong-Min;Kim, Sun-Chang;Im, Wan-Taek
    • Journal of Ginseng Research
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    • v.44 no.6
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    • pp.784-789
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    • 2020
  • Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb3 from ginseng extracts is limited by the co-existence of its isomer Rb2. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb3 from a mixture of isomers. Methods: To isolate Rb3 from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb2 into ginsenoside Rd. Ginsenoside Rb3 was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb3 was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb3 can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures.

Compensation of Magnetometer in the Navigation System for Unmanned Helicopter using an Electric Motor (전기모터를 사용한 소형 무인헬리콥터에 활용될 항법장치용 자장계의 보상)

  • Lee, Gilho;Jo, Sungbeom;Kim, Jungsung;Choi, Keeyoung;Kee, Changdon;Song, Yongkyu;Koo, Wheonjoon
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.40 no.11
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    • pp.997-1003
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    • 2012
  • GNSS and ARS are the most common sensors in low-end UAVs. However, these sensors are vulnerable to built-in errors and cannot measure the body heading independently. The GNSS/INS cannot fully compensate the IMU errors in initial alignment process and rectilinear flights. For an unmanned helicopter, a magnetometer can be more useful than any other sensors to obtain heading information. However, the electric motor which drives small helicopter UAV keeps the magnetometer from reading the pure magnetotelluric vector. This paper shows the effects of electric motor on the magnetometer readings, and presents a method to compensate the effects. The results are verified with flight test data. The simulation and experimental results in this paper proves that aiding GNSS/INS with magnetometer increases observability and improves accuracy.

Retrospective clinical study of multiple keratocystic odontogenic tumors in non-syndromic patients

  • Hwang, Dae-Seok;Kim, Yun-Ho;Kim, Uk-Kyu;Ryu, Mi-Heon;Kim, Gyoo-Cheon
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.44 no.3
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    • pp.107-111
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    • 2018
  • Objectives: A keratocystic odontogenic tumor (KOT) is a type of odontogenic tumor that mainly occurs in the posterior mandible. Most KOTs appear as solitary lesions; however, they sometimes occur as multiple cysts. This study analyzed the clinical features of multiple KOTs. Materials and Methods: The participants were diagnosed with KOT by biopsy with multiple surgical sites, and were patients at the Pusan National University Hospital and the Pusan National University Dental Hospital from January 1, 2005 to March 31, 2016. Charts, records, images and other findings were reviewed. Results: A total of 31 operations were conducted in 17 patients. The mean patient age was $28.4{\pm}20.1years$. Multiple KOTs were found to occur at a young age (P<0.01). The predominant sites were in the posterior mandible (28.6%). Most cases of multiple lesions appeared in both the upper and lower jaw, and 40.3% of lesions were associated with unerupted and impacted teeth. The overall recurrence rate measured by operation site was 10.4% (8/77 sites). No patients were associated with nevoid basal cell carcinoma syndrome. Conclusion: The pure recurrence rate was lower than estimated, but there was a higher possibility of secondary lesions regardless of the previous operation site; therefore, long-term follow-up is necessary.