• Title/Summary/Keyword: Pure sciences

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Isolation of Schwann Cell and Separation of Schwann Cell-Neuron Network from Mouse Embryo (마우스 배아에서 슈반세포-뉴런 네트워크의 분리와 슈반세포의 분리)

  • Kweon, Tae-Dong;Sa, Young-Hee;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2013.10a
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    • pp.943-945
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    • 2013
  • The study of Schwann cell myelination has been facilitated by the availability to isolate and establish pure population of primary Schwann cells. Dorsal root ganglia (DRG) of mouse embryo as source of Schwann cells were used in this study. This method includes three steps: first step of dissociation of the embryonic DRG, second step of expansion of Schwann cell precursors, followed by mechanical separation of the Schwann cell-neuronal network from the underlying fibroblasts, and third step of purification of Schwann cells from the associated neurons and subsequent expansion of the purified Schwann cells. We made a highly purified population of Schwann cells and Schwann cell-neuron networks in a short period using this procedure.

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Impact of energy efficiency improvement on greenhouse gas in off-season tomato farming: Evidence from Punjab, Pakistan

  • Ali, Qamar;Khan, Muhammad T.I.;Khan, Muhammad N.I.
    • Advances in Energy Research
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    • v.5 no.3
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    • pp.207-217
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    • 2017
  • Energy consumption in agriculture is responsible for greenhouse gas emission but it can be reduced after efficient utilization of energy inputs. Therefore, the present study aims for the estimation of energy efficiency and extent of greenhouse gas reduction after benchmarking of inefficient farms in off-season tomato in Punjab province of Pakistan. Primary data were collected from 70 farmers with simple random sampling. By using data envelopment analysis, the average value of technical, pure technical and scale efficiency was 0.80, 0.92 and 0.87, respectively while increasing, constant and decreasing return to scale was observed in 33, 26 and 11 farmers, respectively. Total input energy was reduced by $12,688.91MJ\;ha^{-1}$ (13.89%) if inefficient farms used the energy inputs according to recommendations or benchmarking. A major portion of energy saving comes from fertilizers (68.79%) followed by diesel (15.70%), chemicals (5.91%), machinery (4.37%) and water (4.00%). Total greenhouse gases reduction was $499.17kg\;CO_2\;eq.ha^{-1}$ (14.57%) as a result of improvement in energy efficiency or benchmarking of inefficient farms. Agricultural extension staff should visit the vegetable farms on regular basis and give necessary information about efficient utilization of energy inputs. The government should create awareness about the optimum use of input through seminars and pamphlets.

COSMIC RAY ACCELERATION AT COSMOLOGICAL SHOCKS: NUMERICAL SIMULATIONS OF CR MODIFIED PLANE-PARALLEL SHOCKS

  • KANG HYESUNG
    • Journal of The Korean Astronomical Society
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    • v.36 no.3
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    • pp.111-121
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    • 2003
  • In order to explore the cosmic ray acceleration at the cosmological shocks, we have performed numerical simulations of one-dimensional, plane-parallel, cosmic ray (CR) modified shocks with the newly developed CRASH (Cosmic Ray Amr SHock) numerical code. Based on the hypothesis that strong Alfven waves are self-generated by streaming CRs, the Bohm diffusion model for CRs is adopted. The code includes a plasma-physics-based 'injection' model that transfers a small proportion of the thermal proton flux through the shock into low energy CRs for acceleration there. We found that, for strong accretion shocks with Mach numbers greater than 10, CRs can absorb most of shock kinetic energy and the accretion shock speed is reduced up to $20\%$, compared to pure gas dynamic shocks. Although the amount of kinetic energy passed through accretion shocks is small, since they propagate into the low density intergalactic medium, they might possibly provide acceleration sites for ultra-high energy cosmic rays of $E\ll10^{18}eV$. For internal/merger shocks with Mach numbers less than 3, however, the energy transfer to CRs is only about $10-20\%$ and so nonlinear feedback due to the CR pressure is insignificant. Considering that intracluster medium (ICM) can be shocked repeatedly, however, the CRs generated by these weak shocks could be sufficient to explain the observed non-thermal signatures from clusters of galaxies.

A New Report of Biscogniauxia petrensis Isolated from Mosquitoes in Korea

  • Das, Kallol;Kim, Joung-Ho;Choi, Kwang-Shik;Lee, Seung-Yeol;Jung, Hee-Young
    • The Korean Journal of Mycology
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    • v.48 no.2
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    • pp.87-93
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    • 2020
  • A fungal strain designated KNU-WDM2A2 was isolated from mosquitoes in Gimcheon, Korea. The pure culture was transferred to potato dextrose agar (PDA) and synthetic nutrient agar (SNA) media and attained a diameter of 90 mm after 10 days of incubation at 25℃. The colonies were whitish to light pink and cottony to wooly, with an abundant production of aerial mycelia. The strain produced hyaline to slightly yellowish conidiophores that were rough-walled and branched, with conidiogenous cells arising terminally or laterally. Conidia were unicellular, hyaline to light brown, smooth, and oval or ovoid to clavate, with a size of 4.1-6.9×2.5-3.3 ㎛ (n=65). A phylogenetic analysis was conducted using the internal transcribed spacer (ITS) regions and 28S rDNA of large subunit (LSU) sequences, to support the cultural and morphological characteristics. The KNU-WDM2A2 strain was identified here as Biscogniauxia petrensis, new to Korea.

Effect of Salt Concentration and Temperature in Synthetic Dyestuff Wastewater Treatment using Plate and Frame Membrane Module (평판형 막모듈을 이용한 합성염료 폐수처리에 있어서 염농도 및 온도의 영향)

  • Kim, Sun-Il;Lee, Bong-Woo;Yun, Young-Jae
    • Journal of Environmental Science International
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    • v.7 no.2
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    • pp.209-216
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    • 1998
  • Nanofiltration[NF45] and reverse osmosis membrane(HR98PP) separation treatment of dyestuff wastewater was carried out In order to separate relatively pure water from synthetic dyestuff wastewater, which consists of reactive dye, acid dye, basic dye, direct dye, and disperse dye. The experiments were performed by using the plate and frame membrane module. In the nanofiltration and reverse osmosis membrane separation, When the NaCl concentration was 0.1, 5.0, and 20.091, retention was 63.0, 46.0, 0.9%, respectively. When permeate flux was 125.0, 67.5, and 45.0 L/$m^2$ h, the osmotic pressure increased with Increasing the NaCl concentration. Permeate flux of two membranes Increased as temperature Increased due to segmental movement of polymer of the membrane and the rejection rate of dyestuff was decreased gradually. It was found that the rejection rate was about 95% in the nanofiltratlon, while the reverse osmosis membrane showed a high rejection rate of 99% under all temperature and pressures conditions.

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Stochastic MAC-layer Interference Model for Opportunistic Spectrum Access: A Weighted Graphical Game Approach

  • Zhao, Qian;Shen, Liang;Ding, Cheng
    • Journal of Communications and Networks
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    • v.18 no.3
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    • pp.411-419
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    • 2016
  • This article investigates the problem of distributed channel selection in opportunistic spectrum access networks from a perspective of interference minimization. The traditional physical (PHY)-layer interference model is for information theoretic analysis. When practical multiple access mechanisms are considered, the recently developed binary medium access control (MAC)-layer interference model in the previous work is more useful, in which the experienced interference of a user is defined as the number of competing users. However, the binary model is not accurate in mathematics analysis with poor achievable performance. Therefore, we propose a real-valued one called stochastic MAC-layer interference model, where the utility of a player is defined as a function of the aggregate weight of the stochastic interference of competing neighbors. Then, the distributed channel selection problem in the stochastic MAC-layer interference model is formulated as a weighted stochastic MAC-layer interference minimization game and we proved that the game is an exact potential game which exists one pure strategy Nash equilibrium point at least. By using the proposed stochastic learning-automata based uncoupled algorithm with heterogeneous learning parameter (SLA-H), we can achieve suboptimal convergence averagely and this result can be verified in the simulation. Moreover, the simulated results also prove that the proposed stochastic model can achieve higher throughput performance and faster convergence behavior than the binary one.

The Effects of Lactic Acid Bacteria on the Growth and Aflatoxin $B_1$ Production of Aspergillus parasiticus (유산균이 Aspergillus parasiticus의 성장과 Aflatoxin $B_1$ 생성에 미치는 영향)

  • Lee Kyung-Min;Kim Jong-Gyu
    • Journal of Environmental Health Sciences
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    • v.31 no.2 s.83
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    • pp.127-133
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    • 2005
  • This study was performed to investigate the competitive effect of Leuconostoc mesenteroides and Lactobacillus plantarum on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus. Leu. mesenteroides and Lac. plantarum were grown with A. parasiticus in a modified APT broth at $28^{\circ}C$ for 9 days. It was observed that the growth of A. parasiticus got lower when the two bacteria were inoculated simultaneously than when the mold grew alone. The pH of mixed culture of Leu. mesenteroides and Lac. plantarum showed significantly lower value than the pH of the pure culture of A. parasiticus (p<0.05). The acidity of the mixed culture group significantly increased compared with the control group (A. parasiticus alone group) (p<0.05). The aflatoxin $B_1$ production was significantly decreased in the mixed culture group than in the A. parasiticus alone group (p<0.05). Leu. mesenteroides showed the more efficient effect than Lac. plantarum. These results indicate that the two lactic acid bacteria have inhibitory effect on the growth and aflatoxin $B_1$ production of Aspergillus parasiticus.

Embedded Web Server Implementation for Building Sensor Network Design (빌딩 센서 네트워크 설계를 위한 임베디드 웹서버 구축)

  • Kim, Yong-Ho;Nam, Soung-Youl;Kim, Hyeong-Gyun;Choi, Gwang-Mi
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.2
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    • pp.1015-1018
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    • 2005
  • This study intended to demonstrate a general system as an web server implemented by pure software solution and focused on collecting data by remote control through Internet and constructing its' control frame. To achieve these, this study suggested an optimized, low-power, ultra tiny embedded web server. When unpredictable accidents, such as heat sensor or industrial disasters, happen, it will connect sensors collecting building information each other by network and obtain final results via web porting, web hardware control or porting, or hardware test process in a boot loader on the basis of StrongARM SA-1110 processor.

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First Report of Gray Mold Disease on Endangered Species Cypripedium japonicum

  • Jeon, Chang-Wook;Kim, Da-Ran;Gang, Geun Hye;Kim, Byung-Bu;Kim, Nam Ho;Nam, Seong-Yeol;Kwak, Youn-Sig
    • Mycobiology
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    • v.48 no.5
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    • pp.423-426
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    • 2020
  • Cypripedium japonicum is known to be the indigenous plant to Korea, Japan, and China. However, C. japonicum represents the most critically endangered plant species in South Korea. The plant is esthetically pleasing due to its flower, which is larger than any other orchidaceous species. Disease symptoms relating to gray mold were observed on C. japonicum in May 2019. The suspected pathogen was successfully isolated from the symptomatic leaf tissue and conducted a pure culture of the fungi. The conidia formed consisted of a colorless or light brown single cell, which was either egg or oval-shaped with a size of 7.1 to 13.4 × 5.2 to 8.6 ㎛. Molecular phylogenetic relationship analysis was also confirmed that the pathogen concerned belonging to the family of Botrytis cinerea. Therefore, the findings confirmed that the pathogen isolated from C. japonicum was consistent with the unique properties of B. cinerea.

The extinction probability in systems randomly varying in time

  • Pazsit, Imre;Williams, M.M.R.;Pal, Lenard
    • Nuclear Engineering and Technology
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    • v.49 no.6
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    • pp.1301-1309
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    • 2017
  • The extinction probability of a branching process (a neutron chain in a multiplying medium) is calculated for a system randomly varying in time. The evolution of the first two moments of such a process was calculated previously by the authors in a system randomly shifting between two states of different multiplication properties. The same model is used here for the investigation of the extinction probability. It is seen that the determination of the extinction probability is significantly more complicated than that of the moments, and it can only be achieved by pure numerical methods. The numerical results indicate that for systems fluctuating between two subcritical or two supercritical states, the extinction probability behaves as expected, but for systems fluctuating between a supercritical and a subcritical state, there is a crucial and unexpected deviation from the predicted behaviour. The results bear some significance not only for neutron chains in a multiplying medium, but also for the evolution of biological populations in a time-varying environment.