• Title/Summary/Keyword: Pure sciences

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Acidifier as an Alternative Material to Antibiotics in Animal Feed

  • Kim, Y.Y.;Kil, D.Y.;Oh, H.K.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.7
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    • pp.1048-1060
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    • 2005
  • Dietary acidifiers appear to be a possible alternative to feed antibiotics in order to improve performance of weaning pigs. It is generally known that dietary acidifiers lower gastric pH, resulting in increased activity of proteolytic enzymes, improved protein digestibility and inhibiting the proliferation of pathogenic bacteria in GI tract. It is also hypothesized that acidifiers could be related to reduction of gastric emptying rate, energy source in intestine, chelation of minerals, stimulation of digestive enzymes and intermediate metabolism. However, the exact mode of action still remains questionable. Organic acidifiers have been widely used for weaning pigs' diets for decades and most common organic acidifiers contain fumaric, citric, formic and/or lactic acid. Many researchers have observed that dietary acidifier supplementation improved growth performance and health status in weaning pigs. Recently inorganic acidifiers as well as organic acidifiers have drawn much attention due to improving performance of weaning pigs with a low cost. Several researchers introduced the use of salt form of acidifiers because of convenient application and better effects than pure state acids. However, considerable variations in results of acidifier supplementation have been reported in response of weaning pigs. The inconsistent responses to dietary acidifiers could be explained by feed palatability, sources and composition of diet, supplementation level of acidifier and age of animals.

Spectroscopic Characterization on the Aggregation Behavior of Pyronin G with Tetraphenylborate anion (Tetraphenylborate 음이온과 Pyronin G의 회합에 관한 분광학적 특성)

  • Lee, Beom-Gyu;Kim, Kang-Jin
    • Analytical Science and Technology
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    • v.8 no.1
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    • pp.47-53
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    • 1995
  • The aggregation of Pyronin G($PG^+$) with tetraphenylborate($TPB^-$) anion has been investigated in aqueous solution by absorption and fluorescence spectrophotometries. The addition of 1,4-dioxane in aqueous solution of $PG^+$ and $TPB^-$ dissociates the aggregates of PG-TPB due to the increased hydrophobicity of the mixed solvent as compared to pure water, and yields higher $PG^+$ momomer concentration than aqueous $PG^+$ solution. The aggregation is not observed in any organic solvents except in water. The absorption maxima of $PG^+$ are correlated relatively well with ${\pi}^*$ scale and $(n^2-1)/(2n^2+1)$ funtion. But better linear relationship was obtained with ${\pi}^*$ scale for alcohols.

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Multimeric Expression of the Antimicrobial Peptide Buforin II in Escherichia coli by Fusion to a Cysteine-Rich Acidic Peptide

  • Lee, Jae-Hyun;Kim, Jeong-Hyun;Hong, Seung-Suh;Lee, Hyun-Soo;Kim, Sun-Chang
    • Journal of Microbiology and Biotechnology
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    • v.9 no.3
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    • pp.303-310
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    • 1999
  • A cost-effective mass production method for a strong antimicrobial peptide, buforin II, which was isolated from the stomach of Bufo bufo gargarizans, has been developed. This method is based on the neutralization of the positive charge of buforin II by fusion with a cysteine-rich acidic peptide (CAP) to avoid any lethal effect on the host. The neutralized fusion peptide was multimerized and expressed in Escherichia coli as tandem repeats to increase the production yield. Multimers of the CAP-buforin II fusion peptide were successfully expressed at high levels in E. coli as inclusion bodies. More than 100mg of pure buforin II was obtained per 11 of E. coli culture after cleaving the multimeric polypeptide with CNBr. The buforin II obtained from the recombinant E. coli had antimicrobial activity identical to that of natural buforin II. The proposed expression system can provide a cost-effective mass production method for both antimicrobial peptides and other host-lethal basic proteins.

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PROBABILISTIC ANALYSIS OF A SYSTEM CONSISTING OF TWO SUBSYSTEMS IN THE SERIES CONFIGURATION UNDER COPULA REPAIR APPROACH

  • Raghav, Dhruv;Pooni, P.K.;Gahlot, Monika;Singh, V.V.;Ayagi, Hamisu Ismail;Abdullahi, Ameer Hassan
    • The Pure and Applied Mathematics
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    • v.27 no.3
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    • pp.137-155
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    • 2020
  • Redundancy is commonly employed to improve system reliability. In most situations, components in the standby configurations are assumed statistically similar but independent. In many realistic models, all parts in standby are not treated as identical as they have different failure possibilities. The operational structure of the system has subsystem-1 with five identical components working under 2-out-of-5: G; policy, and the subsystem-2 has two units and functioning under 1-out-of-2: G; policy. Failure rates of units of subsystems are constant and assumed to follow an exponential distribution. Computed results give a new aspect to the scientific community to adopt multi-dimension repair in the form of the copula.

Local Structure Study of Ni in Ni-Zn Alloy Coating on Steel by X-ray Absorption Spectroscopy (X선 흡수 분광법을 이용한 Ni-Zn 도금 강판에서의 Ni의 국부 구조에 관한 연구)

  • Lee, Do-Hyung
    • Analytical Science and Technology
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    • v.11 no.3
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    • pp.202-205
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    • 1998
  • X-ray absorption fine structure spectroscopic studies at the Ni K-edge have been performed for the Ni-Zn alloy coating layer on steel. The Ni-Zn interatomic distances and Debye-Waller factors were determined by fitting the experimental data with the theoretical spectra in the temperature range of 80 to 300K. The average Ni-Zn interatomic distance was found to be $2.557{\AA}$ and the variation of the Ni-Zn interatomic distance with temperature in this range was insignificant. From the comparison of the Ni-Zn interatomic distance with the nearest neighbor distance of pure Zn lattice it has been suggested that there is an apparent contraction around Ni atom.

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Determination of Biotin by HPLC (고성능 액체크로마토크래피를 이용한 Biotin의 정량)

  • Kim, Dong-Soo;Lee, Young-Ja;Jeong, Dong-Youn;Lee, Dong-Yup;Ahn, Moon-Kyu
    • Analytical Science and Technology
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    • v.16 no.6
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    • pp.499-503
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    • 2003
  • A high performance liquid chromatography gradient elution method with fluorescence detection for the determination of biotin in pure form and pharmaceutical preparations has been developed. BrMDMC gives intense fluorescence and the fluorescence was monitored with excitation at 360 nm and emission at 410 nm. The calibration curve for biotin shows good linearity over the range of 5 ~ 400 ng with correlation coefficient of 0.999. The detection limit of biotin was 2 ng and the result of recovery was 98.75% with relative standard deviation of 1.1%.

Preparation and Characterization of Quercetin-Loaded Solid Dispersion by Solvent Evaporation and Freeze-Drying Method

  • Park, Sang Hyun;Song, Im-Sook;Choi, Min-Koo
    • Mass Spectrometry Letters
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    • v.7 no.3
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    • pp.79-83
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    • 2016
  • We prepared solid dispersion formulations of quercetin to enhance its solubility and dissolution rate. Various quercetin-loaded solid dispersion were tested with quercetin, poloxamer 407, and carrier such as hydroxypropyl methyl cellulose (HPMC), polyethylene glycol 8000 (PEG 8000), and polyvinylpyrrolidone K40 (PVP K40) using solvent evaporation and freeze drying methods in terms of both the aqueous solubility and the dissolution rates of quercetin. The solubility of quercetin as its solid dispersion formulations was markedly improved compared with that of quercetin powder. Especially, highest solubility of quercetin was observed when HPMC was used as a carrier. The cumulative dissolution of quercetin within 360 min from solid dispersion composed of quercetin, poloxamer 407, and HPMC was 8.8-fold higher than the dissolution of pure quercetin. The results of powder X-ray diffraction (XRD) and scanning electron microscope (SEM) indicated that quercetin transformed from a crystalline to an amorphous form through the solid dispersion formulation process. These results suggest that the solid dispersion formulation of quercetin with poloxamer 407 and HPMC could be a promising option for enhancing the solubility and dissolution rate of quercetin.

Activated Carbon-Nickel (II) Oxide Electrodes for Capacitive Deionization Process

  • Gandionco, Karl Adrian;Kim, Jin Won;Ocon, Joey D.;Lee, Jaeyoung
    • Applied Chemistry for Engineering
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    • v.31 no.5
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    • pp.552-559
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    • 2020
  • Activated carbon-nickel (II) oxide (AC-NiO) electrodes were studied as materials for the capacitive deionization (CDI) of aqueous sodium chloride solution. AC-NiO electrodes were fabricated through physical mixing and low-temperature heating of precursor materials. The amount of NiO in the electrodes was varied and its effect on the deionization performance was investigated using a single-pass mode CDI setup. The pure activated carbon electrode showed the highest specific surface area among the electrodes. However, the AC-NiO electrode with approximately 10 and 20% of NiO displayed better deionization performance. The addition of a dielectric material like NiO to the carbon material resulted in the enhancement of the electric field, which eventually led to an improved deionization performance. Among all as-prepared electrodes, the AC-NiO electrode with approximately 10% of NiO gave the highest salt adsorption capacity and charge efficiency, which are equal to 7.46 mg/g and 90.1%, respectively. This finding can be attributed to the optimum enhancement of the physical and chemical characteristics of the electrode brought by the addition of the appropriate amount of NiO.

Complex Permittivity of Dry Sand Measured by an Open-Ended Coaxial Probe (개방단말 동축선 프로브로 측정된 마른 모래의 복소 유전율)

  • Shin, Hyun;Kim, Se-Yun;Kim, Young-Sik
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.37 no.5
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    • pp.17-24
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    • 2000
  • Complex permittivity of dry sand is measured by using an open-ended coaxial probe, which was self-designed and manufactured. As a simple calibration scheme of the probe, the phase difference between the measured and the calculated reflection coefficients of pure water is subtracted from the phase of the measured reflection coefficient for dry sand. And then the complex permittivity of dry sand is reconstructed by applying its measured reflection coefficient into an improved imaginary transmission-line model. The accuracy of our measurement scheme is verified by showing that its reconstructed complex permittivity approaches the precise value of dry sand.

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Optimum Dosage of Fenton's Reagent for the Dyeing Wastewater by the Different Conditions of Biological Treatment as the Pre-treatment Process (염색폐수의 생물학적 전처리 조건변화에 의한 최적 펜톤시약 투입량 결정에 관한 연구)

  • Bea Joan-Sam;Lee Sang-Ho
    • Journal of Environmental Science International
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    • v.14 no.7
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    • pp.683-689
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    • 2005
  • The consecutive combination process of a biological process as the pre-treatment and a chemical process as the post-treatment is applied for the dyeing wastewater. The poor efficiency of biological treatment using pure oxygen makes the chemical treatment cost high. It is necessary to improve the efficiency of biological treatment in order to reduce the cost of chemical treatment. The purpose of this paper is to find the minimum dose of chemical reagent to fit the Discharged Water Quality Standards for the different biological treatment effluents. Results revealed that the minimum dosage of Fenton's reagent lead to save the cost of chemical treatment based on the guideline dose in the treatment plant. The possible maximum saving reagents was up to $70\%$ for the effluent of the pilot plant packed with the carrier imbedded microorganisms which were selected from the present treatment plant.