• Animal Bioscience
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• v.35 no.2
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• pp.236-246
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• 2022

Objective: The objective of this study was to investigate the effect of dietary pumpkin (Cucurbita moschata) seed meal (PSM) on laying hens' performance, quality, fatty acids, cholesterol, antioxidant compounds and shelf life of eggs. Methods: Eighty Tetra SL laying hens, 50-week-old, were randomly divided into two equal groups, having 10 replicates with 4 birds in each. The control (CON) treatment was fed with basal diet, while experimental treatment was fed a diet with 9% PSM, for a 6 week period. Results: Dietary PSM significantly decreased average daily feed intake (p<0.05), with no significant effect on other performance parameters. The PSM, enriched the eggs with polyunsaturated fatty acids, especially α linolenic acid (0.33 vs 0.21 g/100 g) and linoleic acid (20.65 vs 18.37 g/100 g), whereas it reduced the amount of arachidonic acid with 3.91% and n-6/n-3 ratio in PSM eggs compared with CON. The inclusion of 9% PSM significantly (p<0.05) diminished the cholesterol concentration in yolk with 11.31% and in egg with 10.38%, in respect to the CON samples. The significantly (p<0.05) higher concentration of polyphenols and antioxidant compounds, determined in PSM eggs, proved to be effective on shelf life of eggs preserved at refrigerator (5℃) and room temperature (21℃) for 28 days, by delaying the lipid oxidation and protein denaturation. This effect was reflected in significantly (p<0.05) higher Haugh unit in eggs stored 28 days at 21℃ and lower albumen pH values for the overall storage time, both at 5℃ and 21℃, proving the antioxidant effect of pumpkin. Conclusion: Dietary PSM supplementation was significantly effective on average daily feed intake and egg quality by increasing some fatty acids while lowering the cholesterol concentration. Also, PSM proved to be effective improving shelf life of eggs for 28 days storage time.

• The Korean Journal of Pesticide Science
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• v.19 no.2
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• pp.119-124
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• 2015

Bacterial leaf spot, caused by Pseudomonas syringae pv. syrinage, is a very damaging disease to green pumpkin in Gong-ju and Non-san nursery. However, there is no good method to control the disease in Korea. Growth inhibition of pathogen on medium, control efficacy on seedling stage, and seed treatment effect of 6 anti-bacterial pesticides were investigated for selection of the best pesticide for seed treatment and control of the disease. Growth inhibition zone on King's B medium were the largest by oxytetracycline 170 ppm and oxytetracycline 15 ppm + streptomycin sulfate 188 ppm, oxolinic acid 200 ppm, streptomycin 200 ppm were next respectively. Control efficacy of oxytetracycline 1.5% + streptomycin sulfate 18.8% WP and oxytetracycline 17% WP on seedling stage were 71.4% and 49.4%, respectively. Seed treatment of oxytetracycline 15 ppm + streptomycin sulfate 188 ppm on the artificially inoculated seeds inhibits pathogen growth completely from the treated seeds and 96% control efficacy on grow-out test of the treated seeds. Seed treatment of streptomycin 100 ppm (2,000 dilution of streptomycin 20%) on the artificially inoculated seeds allow 280 cfu/g of pathogen growth from the treated seeds and 60% control efficacy on grow-out test of the treated seeds. Seed treatment of oxytetracycline 85 ppm (2000 dilution of oxytetracycline 17% WP) on the artificially inoculated seeds allow 80 cfu/g of pathogen growth from the treated seeds and 90% control efficacy on grow-out test of the treated seeds. These results suggested that oxytetracycline 1.5% + streptomycin sulfate 18.8% WP was the best pesticide for seed treatment to control of the bacterial spot disease by Pseudomonas syringae pv. syringae.

• Journal of the East Asian Society of Dietary Life
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• v.24 no.3
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• pp.351-358
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• 2014

Pumpkin seed oil (PSO) was investigated for its parasite elimination activity and efficacy in treating disorders of the prostate gland and urinary bladder. We confirmed the composition of PSO and identified its ability to improve vessels. Gas chromatography coupled with mass spectrometric (GC-MS) system was used for PSO composition analysis. Cytotoxicity and cell proliferation were confirmed by Cell Counting Kit-8 (CCK-8) assay. Nitric oxide(NO) production was measured with Griess reagent, and intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 mRNA expression levels were measured by reverse transcription polymerase chain reaction (RT-PCR). As a result, PSO revealed the presence of several components such as linoleic acid, oleic acid, palmitic acid and stearic acid. Cytotoxic effects of PSO were not observed, and PSO increased nitric oxide production in human umbilical vein endothelial cells (HUVEC). Additionally, TNF-${\alpha}$-induced cell proliferation and ICAM-1 expression in HUVEC were inhibited by PSO treatment, whereas VCAM-1 expression was not significantly reduced. Taken together, these results show that PSO is worthy of study as a candidate food material for improvement of vascular disease.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.4
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• pp.432-438
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• 2013

This study was conducted to compare seed viability among 42 species after ten years of storage in the midterm storage complex ($4^{\circ}C$, 30-40% RH) at the National Agrobiodiversity Center (NAC) Korean genebank maintained by the Rural Development Administration (RDA), Republic of Korea and to suggest the relative seed longevity and suitable monitoring intervals. The germination data from initial tests and after ten years of storage were compared to measure changes in viability during storage. The decline in seed viability varied greatly among seeds from -11.5% for Triticum sp. to 80% for melon. Coriander, crowndaisy, safflower, cosmos, Chinesebellflower, waxgourd, melon, castorbean, Welch-onion, hollyhock, wild barley, and tallfescue showed significant decreases in viability of 34.2%, 73.4%, 36.5%, 30.0%, 40.2%, 71.3%, 80.0%, 65.9%, 45.5%, 51.4%, 53.0%, and 33.5%, respectively. Gardenpea, soybean, perilla, onion, wild rice, Italian-ryegrass, and pepper showed a 15-30% decline in viability, while the viability of morningglory, adzukibean, maize, and Capsicum sp. decreased by 15% to 5%. Chicory, radish, Chinese-cabbage, bottlegourd, watermelon, cucumber, pumpkin, Cucurbita sp., groundnut, kidneybean, clubwheat, sesame, wheat, Triticum sp., rice, barley, orchardgrass, buckwheat, and wild tomato showed changes in viability of <5%. The changes in storage viability also varied within families. The wild types of rice and barley showed rapid viability loss and presented different aspects from cultivars. Since seed viability of species, classified as index 1 or 2, showed germination losses >15% after ten years of storage, a viability test should be conducted with five year intervals, while species with germination loss of <15% (in index 3 or 4) can be retested at ten year intervals.

• Korean Journal of Food Science and Technology
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• v.43 no.2
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• pp.195-199
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• 2011

This study investigated the antioxidant effects and physiological activities of extract from various parts of pumpkin (Cucurbita moschata Duch.) such as leaf, flesh, skin and seed. Different dried parts of C. moschata were boiled in ethanol for three hours, evaporated, and lyophilized with a freeze-dryer. Total polyphenol contents were the highest in leaf ($29.62{\pm}0.88mg$ GAE/g DM). The same result was observed in the measurement of the flavonoid content. The leaf extract of C. moschata had stronger DPPH radical-scavenging activities and ABTS-radical scavenging activities than any other part. Leaf extract of C. moschata was found to have the highest ferric-reducing antioxidant power (FRAP) value. Meanwhile, at 0.1 mg/mL of all extracts higher degree of tyrosinase inhibitory effects than kojic acid was observed (leaf; 49.58%, flesh; 40.20%, skin; 41.30%, seed; 36.79% and kojic acid; 20.70%). The glycation inhibitory activity decreased in the order leaf>skin>flesh>seed. The ${\alpha}$-glucosidase inhibitory activity of seed extract was the highest with a value of 63.58% at the concentration of 1 mg/mL.

• Research in Plant Disease
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• v.13 no.3
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• pp.157-163
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• 2007

Hot water treatment that is the most appropriate seed disinfection method for organic vegetable farming was evaluated in this study. Among the leafy vegetable seeds lettuce that was the most sensitive to hot water was suitable to treat at $45^{\circ}C$ for 25 min, while Chinese cabbage and radish seeds were optimally treated at $50^{\circ}C$ for 25 min. The treatments resulted in similar or higher seed germination rate than non-treated seeds and promoted plant growth. In addition, fungi such as Alternaria, Aspergillus, Penicillium, or Mucor grown on the seeds were suppressed over 90% and the bacterial growth on lettuce seeds reduced 98.5% by the treatment. Among the fruit vegetable seeds pumpkin that was vulnerable to hot water was suitable to treat at $50^{\circ}C$ for 15 min, while cucumber and hot pepper seeds revealed optimum treatment at $50^{\circ}C$ for 25 min as chinese cabbage and radish. The treatment also showed similar or higher seed germination rate and growth than non-treated seeds. Furthermore, fungi such as Rhizopus, Aspergillus, Penicillium or Mucor grown on the seeds reduced from 72.0% to 95.4%. The bacterial growth on cucumber and red pepper seeds was suppressed from 65.5% to 86.0% by the treatment. Results indicated that the hot water treatment is practical for disinfection of organic vegetable seeds and the optimum temperature and soaking time varied among the seeds.

• Journal of Convergence for Information Technology
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• v.10 no.2
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• pp.102-108
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• 2020

Increasing the rice consumption, seven kinds of Nurungji which served as a home meal replacer and snack were developed with some functional materials were added, and their physiochemical characteristics were analyzed. The water binding capacity was the highest in Nurungji with black sesame seed (264.13%), and the hardness was the lowest as 0.36 N in Nurungji with Tenebrio molitor. The reducing sugar content was the highest in Nurungji with sweet pumpkin (1.47%) when being soaked in water for 30 minutes. As a result of measuring 2,2-diphenyl-1-picryl-hydrazyl free radical scavenging activity of the tested Nurungji preparations, Nurungji with sweet pumpkin and beet showed the high scavenging activity against free radicals. As a result of sensory evaluation, overall acceptability was the highest in Nurungji with black sesame seeds (4.3). Physicochemical characterization and sensory evaluation of Nurongji added with various materials confirmed that they could be applied to various food products.

• Korean Journal of Environmental Agriculture
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• v.33 no.4
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• pp.231-238
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• 2014

BACKGROUND: Biochar (BC) has a great potential for enhancing soil fertility and carbon sequestration while facilitating beneficial waste disposition. Therefore, it is essential to assess and mitigate any inadvertent consequences associated with soil biochar amendment. Earthworm activity is very vital in the soil system, yet there are a limited number of studies that have examined their impact resulting from biochar application to soil. METHODS AND RESULTS: In this study, the survival, growth, reproductive tests, and oxidative DNA damage tests (measured by 8-hydroxydeoxyguanosine (8-OHdG) and catalase (CAT) activities) to assess the potential toxicity to earthworm Eisenia fetida in artificial soil amended with BCs were investigated. The BCs derived from perilla meal, sesame meal, and pumpkin seed were pyrolyzed at 300 and $550^{\circ}C$, and then amended with soil at a rate of 5%. All the earthworms survived, but lost weight compared to control soil after 28 day incubation period. Moreover, the BC-amended soils did not significantly affect the cocoon numbers of earthworms. Slightly higher concentrations of 8-OHdG and CAT were observed in earthworms present in BC-treated soil than those in control soil. Furthermore, the 8-OHdG concentrations in the soil amended with BC produced at $550^{\circ}C$ were greater than those at $300^{\circ}C$, and it slightly decreased as the incubation time increased. CONCLUSION: These observations could be due to higher contents of toxic metal(loid)s and also higher pH in BCs pyrolyzed at $550^{\circ}C$ than $300^{\circ}C$. While BC is efficiently being used in agricultural fields, this study suggests that it is required to assess the unintended negative impacts of BC on soil ecosystems.

• The Korean Journal of Mycology
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• v.10 no.1
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• pp.7-13
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• 1982

Habit characteristics of imperfect and perfect stage of D. bryoniae encountered on naturally infected seeds of cucumber and pumpkin were studied by the blotter method and compared with those grown on Difco potato dextrose agar (PDA), V-8 juice agar and water agar leaf medium (WALM). Most of the pycnidiospores obtained from each isolate of this fungus grown on PDA were non-septate and microtype. Non-septate pycnidiospores were predominanted in all isolates, but a macrotype of the non-septate and a number of uniseptate pycnidiospores were produced on V-8 juice agar and water agar leaf medium. On seed the pycnidiospores were mostly non -septate, but rarely uniseptate ones were also found. On radicle of cucumber seed, the pycnidiospores were non-septate and uniseptate but small percentage biseptate with somewhat constricted at septa. Pycnidiospores produced on V-8 juice agar and water agar leaf medium were similar to those produced on seeds. In the present investigation the perithecia were mostly globose to subglobose with apical papillate ostiole and whitish spore masses formed on the ostiole of perithecia, either on naturally infected seed or on culture media. The mature perithecia were dark brown to black. They were partially embedded or erumpent on seed coat and culture media. The perithecia varied in size within a much narrower range than the pycnidia. But perithecial formation of this fungus on PDA, V-8 juice agar, WALM and seed varied considerably depending upon isolate and culture media.

• Research in Plant Disease
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• v.13 no.2
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• pp.82-87
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• 2007

The genetic diagnostic method of virion capture (VC)/RT-PCR was developed for the simultaneous detection of three rod shaped viruses of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus(KGMMV) and Zucchini green mottle mosaic virus (ZGMMV) transmitted by seed in Cucurbit. Out of 12 primer combinations for the three tobamoviruses, a primer set of CGMMV-C724, KGMMV-K513 and ZGMMV-Z407A was useful for mono and triplex VC/RT-PCR. The triplex VC/RT-PCR for the three tobamovirus in Cucurbit could detect specifically without interference among primers and/or plant species of watermelon, gourd, cucumber, melon, pumpkin, squash and Nicotiana benthamiana.