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http://dx.doi.org/10.7585/kjps.2015.19.2.119

Selection of the Antibacterial Agents for Control Against Pseudomonas syringae pv. syringae causing Leaf Spot Disease on Green Pumpkin (Cucurbita moschata)  

Park, Kyoung Soo (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.)
Kim, Young Tak (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.)
Kim, Hye Seong (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.)
Cha, Jea Soon (Department of Plant Medicine, Chungbuk National University)
Park, Kyeong Hun (Ginseng division, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
The Korean Journal of Pesticide Science / v.19, no.2, 2015 , pp. 119-124 More about this Journal
Abstract
Bacterial leaf spot, caused by Pseudomonas syringae pv. syrinage, is a very damaging disease to green pumpkin in Gong-ju and Non-san nursery. However, there is no good method to control the disease in Korea. Growth inhibition of pathogen on medium, control efficacy on seedling stage, and seed treatment effect of 6 anti-bacterial pesticides were investigated for selection of the best pesticide for seed treatment and control of the disease. Growth inhibition zone on King's B medium were the largest by oxytetracycline 170 ppm and oxytetracycline 15 ppm + streptomycin sulfate 188 ppm, oxolinic acid 200 ppm, streptomycin 200 ppm were next respectively. Control efficacy of oxytetracycline 1.5% + streptomycin sulfate 18.8% WP and oxytetracycline 17% WP on seedling stage were 71.4% and 49.4%, respectively. Seed treatment of oxytetracycline 15 ppm + streptomycin sulfate 188 ppm on the artificially inoculated seeds inhibits pathogen growth completely from the treated seeds and 96% control efficacy on grow-out test of the treated seeds. Seed treatment of streptomycin 100 ppm (2,000 dilution of streptomycin 20%) on the artificially inoculated seeds allow 280 cfu/g of pathogen growth from the treated seeds and 60% control efficacy on grow-out test of the treated seeds. Seed treatment of oxytetracycline 85 ppm (2000 dilution of oxytetracycline 17% WP) on the artificially inoculated seeds allow 80 cfu/g of pathogen growth from the treated seeds and 90% control efficacy on grow-out test of the treated seeds. These results suggested that oxytetracycline 1.5% + streptomycin sulfate 18.8% WP was the best pesticide for seed treatment to control of the bacterial spot disease by Pseudomonas syringae pv. syringae.
Keywords
Bacterial leaf spot; Green pumpkin; Pseudomonas syringae pv. syringae; Seed treatment;
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