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Analysis of Fatty Acid Composition and Effects of Pumpkin Seed Oil on Human Umbilical Vein Endothelial Cells  

Kim, Kyoung Kon (Dept. of Bio-Health Technology, Kangwon National University)
Kang, Yun Hwan (Well-being Bioproducts RIC, Kangwon National University)
Kim, Dae Jung (Well-being Bioproducts RIC, Kangwon National University)
Kim, Tae Woo (Well-being Bioproducts RIC, Kangwon National University)
Lee, Jeong Il (Basic Lab., Hanbit Flavor & Frganace)
Choe, Myeon (Dept. of Bio-Health Technology, Kangwon National University)
Publication Information
Journal of the East Asian Society of Dietary Life / v.24, no.3, 2014 , pp. 351-358 More about this Journal
Abstract
Pumpkin seed oil (PSO) was investigated for its parasite elimination activity and efficacy in treating disorders of the prostate gland and urinary bladder. We confirmed the composition of PSO and identified its ability to improve vessels. Gas chromatography coupled with mass spectrometric (GC-MS) system was used for PSO composition analysis. Cytotoxicity and cell proliferation were confirmed by Cell Counting Kit-8 (CCK-8) assay. Nitric oxide(NO) production was measured with Griess reagent, and intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 mRNA expression levels were measured by reverse transcription polymerase chain reaction (RT-PCR). As a result, PSO revealed the presence of several components such as linoleic acid, oleic acid, palmitic acid and stearic acid. Cytotoxic effects of PSO were not observed, and PSO increased nitric oxide production in human umbilical vein endothelial cells (HUVEC). Additionally, TNF-${\alpha}$-induced cell proliferation and ICAM-1 expression in HUVEC were inhibited by PSO treatment, whereas VCAM-1 expression was not significantly reduced. Taken together, these results show that PSO is worthy of study as a candidate food material for improvement of vascular disease.
Keywords
Pumpkin seed oil; composition assay; nitric oxide production; cell proliferation; intercellular adhesion molecule-1; food material;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Jang HD, Kim CS, Baek KG, Ryu SR (2003) The studies on the analyses of sterols from pumpkin seed oil. Appl Chem 7: 687-690.
2 El-Mosallamy AE, Sleem AA, Abdel-Salam OM, Shaffie N, Kenawy SA (2012) Antihypertensive and cardioprotective effects of pumpkin seed oil. J Med Food 15: 180-189.   DOI   ScienceOn
3 Fleming I, Busse R (2003) Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol 284: R1-R12.   DOI   ScienceOn
4 Fotis L, Giannakopoulos D, Stamogiannou L, Xatzipsalti M (2012) Intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 in children. Do they play a role in the progression of atherosclerosis? Hormones(Athens) 11: 140-146.
5 Fujita Y, Yoshizumi M, Izawa Y, Ali N, Ohnishi H, Kanematsu Y, Ishizawa K, Tsuchiya K, Tamaki T (2006) Transactivation of fetal liver kinase-1/kinase-insert domain-containing receptor by lysophosphatidylcholine induces vascular endothelial cell proliferation. Endocrinology 147: 1377-1385.   DOI   ScienceOn
6 Horowitz A, Menice CB, Laporte R, Morgan KG (1996) Mechanisms of smooth muscle contraction. Physiol Rev 76: 967-1003.
7 Buys E, Sips P (2014) New insights into the role of soluble guanylate cyclase in blood pressure regulation. Curr Opin Nephrol Hypertens 23: 135-142.   DOI
8 Ciapaite J, van Bezu J, van Eikenhorst G, Bakker SJ, Teerlink T, Diamant M, Heine RJ, Krab K, Westerhoff HV, Schalkwijk CG (2007) Palmitate and oleate have distinct effects on the inflammatory phenotype of human endothelial cells. Biochim Biophys Acta 1771: 147-154.   DOI   ScienceOn
9 Kang YH, Kang JS, Shin HM (2013) Vasodilatory effects of cinnamic acid via the nitric oxide-cGMP-PKG pathway in rat thoracic aorta. Phytother Res 27: 205-211.   DOI   ScienceOn
10 Kim DS (2012) The quality characteristics of powder pumpkin soup by different varieties of pumpkins and addition ratios. Korean J Culinary Res 18: 65-76.
11 Kurzen H, Schmitt S, Naher H, Mohler T (2003) Inhibition of angiogenesis by non-toxic doses of temozolomide. Anticancer Drugs 14: 515-522.   DOI   ScienceOn
12 Procida G, Stancher B, Cateni F, Zacchigna M (2012) Chemical composition and functional characterization of commercial pumpkin seed oil. J Sci Food Agric 93: 1035-1041.
13 Livingstone KM, Givens DI, Jackson KG, Lovegrove LA (2014) Comparative effect of dairy fatty acids on cell adhesion molecules, nitric oxide and relative gene expression in healthy and diabetic human aortic endothelial cells. Atherosclerosis 234: 65-72.   DOI   ScienceOn
14 Lu X, Yu H, Ma Q, Shen S, Das UN (2010) Linoleic acid suppresses colorectal cancer cell growth by inducing oxidant stress and mitochondrial dysfunction. Lipids Health Dis 9:106.   DOI   ScienceOn
15 Naito Y, Yoshikawa T (2009) Green tea and heart health. J Cardiovasc Pharmacol 54: 385-390.   DOI   ScienceOn
16 Sales-Campos H, Souza PR, Peghini BC, da Silva JS, Cardoso CR (2013) An overview of the modulatory effects of oleic acid in health and disease. Mini Rev Med Chem 13: 201-210.
17 Saraswati S, Kumar S, Alhaider AA (2013) $\alpha$-santalol inhibits the angiogenesis and growth of human prostate tumor growth by targeting vascular endothelial growth factor receptor 2-mediated AKT/mTOR/P70S6K signaling pathway. Mol Cancer 12: 147.   DOI   ScienceOn
18 Wang S, Chen Y, He L, Yang Y, Chen J, Wang X (2007) Inhibition of vascular smooth muscle cell proliferation by serum from rats treated orally with Gastrodia and Uncaria decoction, a traditional Chinese formulation. J Ethnopharmacol 114: 458-462.   DOI   ScienceOn
19 Tousoulis D, Charakida M, Stefanadis C (2006) Endothelial function and inflammation in coronary artery disease. Heart Apr 92: 441-444.
20 Tousoulis D, Kampoli AM, Tentolouris C, Papageorgiou N, Stefanadis C (2012) The role of nitric oxide on endothelial function. Curr Vasc Pharmacol 10: 4-18.   DOI
21 Zhang B, Ouyang P, Chen Y, Lai WY, Xie JG, Xu DL (2007) Tumor necrosis factor-alpha regulates the proliferation and syndecan-4 expression of human umbilical vein endothelial- like cells cultured in vitro. Nan Fang Yi Ke Da Xue Xue Bao 27: 496-498.
22 Weidemann AK, Crawshaw AA, Byrne E, Young HS (2013) Vascular endothelial growth factor inhibitors: investigational therapies for the treatment of psoriasis. Clin Cosmet Investig Dermatol 6: 233-244.
23 Yamamoto Y, Ishizu A, Ikeda H, Otsuka N, Yoshiki T (2004) Dexamethasone increased plasminogen activator inhibitor-1 expression on human umbilical vein endothelial cells: an additive effect to tumor necrosis factor-alpha. Pathobiology 71: 295-301.   DOI   ScienceOn
24 Yamasaki E, Soma Y, Kawa Y, Mizoguchi M (2003) Methotrexate inhibits proliferation and regulation of the expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 by cultured human umbilical vein endothelial cells. Br J Dermatol 149: 30-38.
25 Zhao G, Etherton TD, Martin KR, Vanden Heuvel JP, Gillies PJ, West SG, Kris-Etherton PM (2005) Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells. Bio chem Biophys Res Commun 336: 909-917.   DOI   ScienceOn
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