• Molecules and Cells
• /
• v.38 no.2
• /
• pp.180-186
• /
• 2015

Escherichia coli AcrAB-TolC is a multidrug efflux pump that expels a wide range of toxic substrates. The dynamic nature of the binding or low affinity between the components has impeded elucidation of how the three components assemble in the functional state. Here, we created fusion proteins composed of AcrB, a transmembrane linker, and two copies of AcrA. The fusion protein exhibited acridine pumping activity, suggesting that the protein reflects the functional structure in vivo. To discern the assembling mode with TolC, the AcrBA fusion protein was incubated with TolC or a chimeric protein containing the TolC aperture tip region. Three-dimensional structures of the complex proteins were determined through transmission electron microscopy. The overall structure exemplifies the adaptor bridging model, wherein the funnel-like AcrA hexamer forms an intermeshing cogwheel interaction with the ${\alpha}$-barrel tip region of TolC, and a direct interaction between AcrB and TolC is not allowed. These observations provide a structural blueprint for understanding multidrug resistance in pathogenic Gram-negative bacteria.

• Progress in Superconductivity and Cryogenics
• /
• v.3 no.2
• /
• pp.15-20
• /
• 2001

This paper describes a model flux pump experiment recently performed at the MIT Francis Bitter Magnet Laboratory. The results of the model flux pump will be used in the development of a prototype flux pump that will be couple to a high-temperature superconductor (HTS) insert coil of a high-field NMR (Nuclear Magnetic Resonance) magnet, Such an HTS insert is unlikely to operate in persistent model because of the conductors low index(n) The flux pump can compensate fro field decay in the HTS insert coil and make the insert operate effectively in persistent mode . The flux pump, comprised essentially of a transformer an two switches. all made of superconductor, transfers into the insert coil a fraction of a magnetic energy that is first introduced in the secondary circuit of the transformer by a current supplied to the primary circuit. A model flux pump has been designed. fabricated, and operated to demonstrate that a flux pump can indeed supply a small metered current into a load superconducting magnet. A current increment in the range of microamperes has been measured in the magnet after each pumping action. The superconducting model flux pump is made of Nb$_3$ Sn tape, The pump is placed in a gaseous environment above the liquid helium level to keep its heat dissipation from directly discharged in the liquid: the effluent helium vapor maintains the thermal stability of the flux pump.

• Korean Journal of Optics and Photonics
• /
• v.5 no.2
• /
• pp.245-251
• /
• 1994

The charateristics of switching between two modes in a ring dye laser has been analyzed by the Monte-Carlo computer simulation. The effect of including pump fluctuations in the first-passage-time (FPT) distributions was compared with the distribution with the quantum fluctuation. The results show the same tendency in both cases, such as steep increases from 0 to peak an exponential decrease in long time range. However the introduction of pumping fluctuation is turned out to shorten the mean FPT. The variation of the mean FPT is examined for the various fluctuationrelated parameters. The mean FPT is lengthened when pump parameter a is increased while it is shorted when Q. $\GAMMA$ are decreased. eased.

• Korean Journal of Optics and Photonics
• /
• v.5 no.4
• /
• pp.461-465
• /
• 1994

The spatial flatness of dye jet depending on the Reynold's number has analyzed by using a Michelson interferometer. When the Reynold's number of dye jet was 600, the flatness of the flow was the best. Under this condition, the dye laser with Littman-type configuration has been operated in a single longitudinal mode at the pumping energy of 0.5~1.0 mJ/pulse. The conversion efficiency and beam divergence were 4.7% and 3 mrad, respectively. ively.

• Ocean and Polar Research
• /
• v.37 no.2
• /
• pp.149-159
• /
• 2015

The Southern Ocean (SO) plays a primary role in global climate by storing and transporting anthropogenic carbon dioxide through the meridional overturning circulation and the biological pumping process. In this study, we aim to investigate interannual variability of summer chlorophyll concentration in the SO and its relation with the El $Ni{\tilde{n}}o$ Southern Oscillation (ENSO), using satellite ocean color data covering 16 years from 1997 to 2012. During El $Ni{\tilde{n}}o$ periods, chlorophyll concentration tends to increase in the subtropics (north of the subantarctic front). This chlorophyll increase is likely linked to El $Ni{\tilde{n}}o$-induced surface cooling that increases nutrient supply through enhanced vertical mixing in the subtropics. On the other hand, the subpolar gyres show localized chlorophyll changes in response to the ENSO. The localized response seems to be primarily attributed to changes in sea-ice concentrations. Our findings suggest that ENSO contributes interannual variability of chlorophyll in the SO through different mechanisms depending on regions.

• Korean Journal of Optics and Photonics
• /
• v.11 no.3
• /
• pp.171-178
• /
• 2000

We present a numerical model which enables to analyze $1.47mu$m amplification band characteristics for thulium doped fluoride fiber amplifiers. We focused on upconversion pumping scheme, thus many transitions affecting $1.47mu$m band amplification was considered simultaneously. Backward propagating waves and transverse mode were also considered in the model. The parameters for modeling were then acquired using published experimental data and related theories such as Judd-Ofelt theory for radiative transition, empirical energy gap law for non-radiative transition, and McCumber relations for cross-sections. The simulation showed well-matched results with experiment and internal dynamics. amics.

• ETRI Journal
• /
• v.24 no.2
• /
• pp.81-96
• /
• 2002

This paper describes our design of a hybrid amplifier composed of a distributed Raman amplifier and erbium-doped fiber amplifiers for C- and L-bands. We characterize the distributed Raman amplifier by numerical simulation based on the experimentally measured Raman gain coefficient of an ordinary single mode fiber transmission line. In single channel amplification, the crosstalk caused by double Rayleigh scattering was independent of signal input power and simply given as a function of the Raman gain. The double Rayleigh scattering induced power penalty was less than 0.1 dB after 1000 km if the on-off Raman gain was below 21 dB. For multiple channel amplification, using commercially available pump laser diodes and fiber components, we determined and optimized the conditions of three-wavelength Raman pumping for an amplification bandwidth of 32 nm for C-band and 34 nm for L-band. After analyzing the conventional erbium-doped fiber amplifier analysis in C-band, we estimated the performance of the hybrid amplifier for long haul optical transmission. Compared with erbium-doped fiber amplifiers, the optical signal-to-noise ratio was calculated to be higher by more than 3 dB in the optical link using the designed hybrid amplifier.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.8 no.1
• /
• pp.114-122
• /
• 1996

Pipeline transport of dredged soils has been widely used for reclamation. In this case the fluid mixed with soils, so called slurries, has very much different characteristics from pure fluids. As the slurry fluid has a peculiar mode in the viscosity, a proper equation of friction factor has to be used which is pertinent to the characteristics of slurry flow for the estimation of pipeline transport of dredged soils. The slurry fluid has the characteristics of Newtonian fluid or non-Newtonian fluid largely depending on the size of particles. In the present study, new forms of pipe friction factor equations have been suggested for both conditions, and using these forms explicit equations have been developed for the computation of relevant pipe diameter and discharge as well as pumping power.

• Particle and aerosol research
• /
• v.6 no.3
• /
• pp.139-145
• /
• 2010

In this study, we investigated the particle formation during the deposition of borophosphosilicate glass (BPSG) and phosphosilicate glass (PSG) films in thermal chemical vapor deposition reactor using in-situ particle monitor (ISPM) and particle beam mass spectrometer (PBMS) which installed in the reactor exhaust line. The particle current and number count are monitored at set-up, stabilize, deposition, purge and pumping process step in real-time. The particle number distribution at stabilize step was measured using PBMS and compared with SEM image data. The PBMS and SEM analysis data shows the 110 nm and 80 nm of mode diameter for BPSG and PSG process, respectively.

• Korean Journal of Optics and Photonics
• /
• v.21 no.5
• /
• pp.185-189
• /
• 2010

We demonstrate a single-frequency wavelength tunable erbium-doped fiber (EDF) ring laser. We used an unpumped-EDF as a saturable-absorber in order to obtain a stable single-frequency with a narrow-linewidth single-polarization mode in the ring cavity. The lasing wavelength was controlled by using bending-induced strain, such as tension and compression strain corresponding to the bending direction, applied to the fiber gratings. The fiber laser exhibited an output power of -1.85 dBm at a wavelength of 1540.72 nm for a pumping power of ~400 mW. An extinction ratio was measured to be more than 60 dB. The proposed tunable fiber laser maintains nearly the same output power while its lasing wavelength is controlled over in a wavelength range of 5 nm.