• International Journal of Oral Biology
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• 제38권4호
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• pp.161-167
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• 2013

Human dental pulp stem cells (DPSCs) are multi-potent mesenchymal stem cells that have several differentiation potentials. An understanding of thetissues that differentiate from these cells can provide insights for future regenerative therapeutics and tissue engineering strategies. The mesiodens is the most frequent form of supernumerary tooth from which DPSCs can differentiate into several lineages similar to cells from normal deciduous teeth. Recently, it has been shown that nanoscale structures can affect stem cell differentiation. In our presentstudy, we investigated the effects of a 250-nm nanoscale ridge/groove pattern array on the osteogenic and adipogenic differentiation of dental pulp cells from mesiodenscontaining human DPSCs. To this end, the expression of lineage specific markers after differentiation induction was analyzed by lineage specific staining and RT-PCR. The nanoscale pattern arrayed surface showed apositive effect on the adipogenic differentiation of DPSCs. There was no difference between nanoscale pattern arrayed surface and conventional surface groups onosteogenic differentiation. In conclusion, the nanoscale ridge/groove pattern arrayed surface can be used to enhance the adipogenic differentiation of DPSCs derived from mesiodens. This finding provides an improved understanding of the effects of topography on cell differentiation as well as the potential use of supernumerary tooth in regenerative dental medicine.

• Archives of Plastic Surgery
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• 제43권1호
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• pp.66-70
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• 2016

Background In this study, we characterize the morbidity at the donor-site of partial second toe pulp free flaps in terms of wound management as well as long-term outcomes. Methods A single-institutional retrospective review was performed for patients who had undergone partial second toe pulp free flap transfer to the fingertip. Patient charts were reviewed for infection, skin necrosis, wound dehiscence, and hematoma for the donor site. Additionally, a questionnaire survey was given to patients who had a follow-up of longer than 1 year to characterize long-term postoperative pain and appearance. Results The review identified a total of 246 cases. Early wound complications were significant for wound dehiscence (n=8) and hematoma (n=5) for a wound complication rate of 5.3%. The questionnaire was distributed to 109 patients, and 54 patients completed the survey. Out of these 54 patients, 15 patients continued to have donor-site pain (28%) at a mean follow-up period of 32.4 months. However, the pain intensity was relatively low in the range between 2 to 5, on a 0-10 scale. None of these patients felt this donor-site pain interfered significantly with daily activity, nor did any patient require pain medications of any type. Donor-site appearance was satisfactory to most patients. Conclusions The partial second toe pulp flap was associated with low rates of wound complications and favorable long-term outcomes. Given the functional and aesthetic gain in the recipient finger, donor-site morbidities appear acceptable in this patient population. This study can be helpful in counseling patients regarding donor-site morbidity during the informed consent process.

• 대한소아치과학회지
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• 제25권4호
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• pp.843-848
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• 1998

Pulpotomy is a frequently used treatment modality in primary teeth. It is method by which infected coronal pulp is removed while retaining vital radicular pulp. Since its introduction in 1930 by Sweet formocresol remains the most popular medicament for this treatment. However, despite its outstanding bactericidal properties, formocresol is known to cause adverse tissue reactions. Theoretically, formocresol disinfects and fixes radicular pulp and thus prevents infection and internal resorption. In reality, however, it leads to chronic inflammation and is sometimes responsible for failures through abscess formation and internal root resorption. Also, Myers et al., in 1978, reported on the systemic distribution of FC and other studies have followed with reports of its immunological, mutagenic and carcinogenic effects. Much effort has, therefore, focused on the development of alternative medicaments and techniques. Since its introduction in 19C, ferric sulfate proven itself as an effective hemostatic agent and is used as an astringent in dentistry. In 1988, Landau and Johnsen suggested ferric sulfate be used as a medicament in pulpotomy and many studies have focused on it to overcome the toxic effects of FC. Ferric sulfate acts through its ferric ion and iron ion, which react with blood protein leading to aggregation. The aggregated protein acts to plug the blood vessels, causing mechanical hemostasis. As blood clot formation is minimal, there is reduced inflammation of radicular pulp and enhanced healing. There are no reports regarding its systemic distribution. This is a report of cases treated by the author using pulpotomy with ferric sulfate.

• 구강회복응용과학지
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• 제25권2호
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• pp.191-200
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• 2009

줄기세포는 미분화 상태의 세포로 지속적인 자가 분열능과 특정 세포로 분화 할 수 있는 분화능을 지니고 있으며 이러한 특성으로 말미암아 난치병의 새로운 장을 열 것이라는 기대감을 얻고 있다. 즉, 세포이식 후 특정 세포로 분화 유도를 시켜 기존의 치료 방법으로는 치료가 되지 않았던 많은 의학적 난제들을 풀 수 있는 열쇠인 것이다. 치아는 평생에 걸쳐 발거되는 조직으로 치아줄기세포는 얻기가 비교적 쉬워서 다른 성체줄기세포들보다 더 높이 평가되고 있다. 과잉치는 전 세계 다양한 인종에서 발견되는 중요한 임상적 질환이다. 과잉치의 유병율은 관련 연구 보고서마다 다양하나 소아치과 의사들의 경우 임상에서 자주 과잉치를 발거하고 있다. 그러나, 현재까지 진행된 줄기세포 관련 연구들을 살펴보면 과잉치의 줄기세포에 관한 보고는 없으며, 이는 줄기세포로 이용 가능한 중요한 자원을 파기하고 있는 것은 아닌지 의문이 생긴다. 그러므로 본 연구는 과잉치에 포함된 세포가 줄기세포의 특징을 가지고 있는지를 밝혀내기 위해 진행되었다.

• Restorative Dentistry and Endodontics
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• 제22권2호
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• pp.710-719
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• 1997

Aucubin, an iridoid glucoside, which is isolated from Aucuba japonica, has some biological effects. This study was to investigate the effect of aucubin on the remainig pulp tissues after pulpotomy. Mongrel dog's coronal pulps were mechanically exposed with a sterile round bur and excised with sterile sharp excarvator. After bleeding was controlled, in control group, $Ca(OH)_2$ powder was applied on the remaining pulps and the cavities were sealed with Z.O.E. cement. In experimental group 1, mixed powder with $Ca(OH)_2$ and aucubin(l : 1 by weight) was applied on the pulpotomized pulp surfaces. After the cavities were covered with sterile aluminum foil, they were sealed with Z.O.E. cement. In experimental group 2, only aucubin powder was applied on the remaining pulps and then they were treated the same as experimental group 1. In the all groups, the pulps were histopathologically observed by light microscope at the time intervals of 1, 2 and 4 weeks after experiment. The results were as follows : 1. In control and experimental groups, mild vascular congestion and bleeding were found in most of the specimens. Less inflammatory infiltration was observed in experimental groups than in control group. 2. Dentin bridge formation was found after 1 week at both control and experimental group 1. Dentin birdge had discontinuous osteodentin like appearance or contained some dentin chips. In experimental group 2, dentin bridge was not seen. 3. The coagulation necrosis layer on the remaining pulp tissues was seen in all groups. In experimental group 2, the thickest layer was observed. And in control group, coagulation necrosis layer was similar as in experimental group 1.

• Restorative Dentistry and Endodontics
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• 제10권1호
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• pp.43-53
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• 1984

The purpose of this study was to suggest the use of laser energy in the the field of operative dentistry without considerable pulpal damage and significant effects on the dental hard tissue, additionally to find out the methods which could control the temperature rise. The laser beam (CW $CO_2$ laser, output: 6W, beam diameter: 1.5mm) was focused on the center of the occlusal surface of extracted lower molars. A Ge lens (focal length 200mm) was used to focus the primary laser beam. In order to vary the total amount of the same irradiated energy, experimental subjects were devided into three groups: continuously irradiated group, intermittently irradiated group, and water-cooled group after continuous laser irradiation. Temperature changes in the pulp chamber after laser irradiation were measured and recorded by the digital thermometer and recorder. The following results were obtained: 1. Temperatures in the pulp chamber were raised up in the order of the continuously irradiated group, intermittently irradiated group, water-cooled group after continuous laser irradiation. 2. In the continuously irradiated group, the temperature was raised up $1.7^{\circ}C$, $3.8^{\circ}C$, $7.3^{\circ}C$, $17.2^{\circ}C$ after 2, 4, 8, 16 seconds of the irradiation of laser. In the intermittently irradiated group, the changes were $1.2^{\circ}C$, $3.4^{\circ}C$, $6.3^{\circ}C$, $11.1^{\circ}C$, respectively. In the water-cooled group after continuous laser irradiation, the changes were $0.0^{\circ}C$, $0.8^{\circ}C$, $1.6^{\circ}C$, $6.9^{\circ}C$, respectively. 3. The starting time of temperature rise in the pulp chamber had no connection with laser irradiation time.

• 대한치과교정학회지
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• 제37권5호
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• pp.351-363
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• 2007

유성견에서 periodontal distraction 과정을 통해 급속 견인 후에 치수와 치주조직의 변화를 평가하였다. 유성견의 상악 제2소구치를 발거하고 제3소구치 근심의 치간골을 부분 제거하였다. 견인장치는 6일 동안 하루에 2번씩 견인하고, 강화기 동안 0주, 1주, 3주, 5주, 7주, 9주에 치수와 치주조직의 변화를 임상적, 방사선학적, 조직학적, 면역조직화화학적으로 관찰하였다. 신장된 치주인대 내에서 견인 직후부터 4주까지 활발한 골밀도 증가를 보였으며, 특히 2-3주 사이에서 가장 활발하였다. 강화기 0주에 압박측 치조골의 흡수 및 파골세포 출현, 염증세포 침윤이 가장 많이 관찰되었고 1주의 신장측 치주인대에서 특징적인 골형성을 보이기 시작하였다. 신생골 형성은 1주와 3주에 가장 많이 관찰되었으며 9주에는 성숙골로의 대치 및 치주인대의 재생으로 대조군과 차이가 없었다. 실험군의 calcitonin gene-related peptide의 발현은 치수, 치주인대 내의 염증 부위에서 나타났으며 0주, 1주에 압박측 치주인대에서 증가하였다가 5주 이후에는 감소하여 대조군과 차이가 없었다. 이상과 같은 소견은 periodontal distraction에 의한 급속 치아 이동이 정상적인 골 재형성 과정을 도모하는 새로운 치아이동 방법이 될 수 있음을 시사한다.

• Journal of Oral Medicine and Pain
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• 제33권1호
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• pp.59-66
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• 2008

사람 Y염색체상에 존재하는 RPS4Y(Ribosomal Protein S4Y) 유전자는 성별감정시 유용한 유전자로 규명되어 유전질환의 조기 발견이나 예방 및 태아의 성별판정 등에 응용되고 있다. 신원이 불분명한 사체에서 성별감정시, 기존의 성별감정에 이용되고 있는 다른 유전자와 함께 RPS4Y 유전자를 검색함으로써 성별감정의 신뢰도를 높힐 수 있을 것으로 사료된다. 또한 사체의 손상이 심할 때 유전자를 이용한 개인식별은 제한을 받게 된다. 이때 치아는 인체의 기관 중 가장 견고한 구조로 구성되어 있어 외부 환경에 대한 물리적, 화학적 저항성이 높아 법의치과학적 개인식별에 널리 이용되므로, 본 연구에서는 사람 치아에서 중합효소연쇄반응법을 이용한 RPS4Y유전자를 검출하여 법의학적 성별감정에 응용하고자 하였다. 남녀 각각 10개의 치아에서 치수와 상아질을 분리한 후 DNA를 추출하여 중합효소연쇄반응을 시행하였다. RPS4Y 유전자를 검출한 결과, 남자에게서만 특이적으로 유전자가 검출되었으며, 이는 사람 치아에서 RPS4Y 유전자를 이용한 성별감정이 법의학적 개인식별의 성별감정 실무에 있어서 다른 유전자와 함께 유용하게 사용될 수 있을 것으로 사료된다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제39권2호
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• pp.55-62
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• 2013

Bone tissue engineering is one of the important therapeutic approaches to the regeneration of bones in the entire field of regeneration medicine. Mesenchymal stem cells (MSCs) are actively discussed as material for bone tissue engineering due to their ability to differentiate into autologous bone. MSCs are able to differentiate into different lineages: osteo/odontogenic, adipogenic, and neurogenic. The tissue of origin for MSCs defines them as bone marrow-derived stem cells, adipose tissue-derived stem cells, and, among many others, dental stem cells. According to the tissue of origin, DSCs are further stratified into dental pulp stem cells, periodontal ligament stem cells, stem cells from apical papilla, stem cells from human exfoliated deciduous teeth, dental follicle precursor cells, and dental papilla cells. There are numerous in vitro/in vivo reports suggesting successful mineralization potential or osteo/odontogenic ability of MSCs. Still, there is further need for the optimization of MSCs-based tissue engineering methods, and the introduction of genes related to osteo/odontogenic differentiation into MSCs might aid in the process. In this review, articles that reported enhanced osteo/odontogenic differentiation with gene introduction into MSCs will be discussed to provide a background for successful bone tissue engineering using MSCs with artificially introduced genes.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.171-171
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• 2004