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Usefulness of RPS4Y Gene on Sex Determination in Human Teeth  

Yoon, Wang-Ro (Department of Oral Medicine, College of Dentistry, Chosun University)
Ahn, Jong-Mo (Department of Oral Medicine, College of Dentistry, Chosun University)
Yoon, Chang-Lyuk (Department of Oral Medicine, College of Dentistry, Chosun University)
Publication Information
Journal of Oral Medicine and Pain / v.33, no.1, 2008 , pp. 59-66 More about this Journal
Abstract
Ribosomal Protein S4Y(RPS4Y) gene is the human sex-linked gene on the Y chromosome. There are a number of reports on the sex determination using RPS4Y gene analysis for prevention and diagnosis in sex-linked disease. Thus RPS4Y gene is a reliable genetic marker for sex determination in forensic medicine. In general, the sex determination of an unidentified body can be achieved based on anatomical characteristics, but sometimes sex determination was considered to be difficult such as pre-adolescent bodies or decomposed, mutilated bodies. In this case, Sex determination using PCR method in human teeth produces good results. Because human teeth have a great structural durability, the DNA well preserved in the teeth. So author isolated nuclear DNA from the 20 human teeth(10 males, 10 females), performed to detect RPS4Y gene by PCR method. Samples were divided four group(10 pulp and 10 dentinal tissue in male, 10 pulp and 10 dentinal tissue in female). It was found that detection of RPS4Y gene for sex determination was possible in all the male pulp tissues and 6 out of 10 male dentinal tissues. But there was not detected in female pulp and dentinal tissues. In the view of this results demonstrates the possibility that detection of RPS4Y gene with other sex chromosome genes from the human teeth is useful to sex determination in forensic medicine.
Keywords
Sex determination; Teeth; RPS4Y gene;
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1 Akane, A., Seki, S., Shiono, H. et al. Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene. Forensic Science International 1992;52:143-148   DOI   ScienceOn
2 Wachtel SS. Moleculr genetics of sex determination. Academic Press Inc. 1994;2-22
3 Gubbay, J., Collignon, J., Koopman, PA. et al. A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes. Nature 1990;346:240-244   DOI   ScienceOn
4 Haga M. A study of sex distinction in the teeth. Jpn. J. Legal Med. 1959;13:582-590
5 Tsutomu Ogata, Chris Tyler-Smith. Chromosomal localization of a gene(s) for Turner stigmata on Yp. J. Med Genet. 1993;30:918-922   DOI
6 Vijay K. Dasari, Reza Z. Goharderakhshan. et al. Expression analysis of Y chromomsome genes in human prostate cancer. The Journal of Urology 2001;165:1335-1341   DOI   ScienceOn
7 Yun-Fai Chris Lau, Jianqing Zhang. Expression analysis of thirty one Y chromosome genes in human prostate cancer. Molecular Carcinogenesis 2000;27:308-321   DOI   ScienceOn
8 Marquis P. Vawter, Simon Evans. Gender-specific gene expression in post-mortem human brain : Localization to sex chromosomes. Neuropsychopharmacology 2004;29:373-384   DOI   ScienceOn
9 Schwartz, TR., Schwartz, EA. Characterization of deoxyribonucleic acid obtained from teeth subjected to various environmental conditions. J. of Forensic Science 1991;36(4):979-990
10 Pfitzinger, H., Ludes, B., Mangin, P. Sex determination of forensic samples : co-amplification and sumultaneous detection of a Y-specific and an X-specific DNA sequence. International J. of Legal Medicine 1993;105: 213-216   DOI
11 Akane, A., Shiono, H., Matsubara. K. et al. Purification of forensic specimens for the polymerase chain reaction(PCR) analysis. J. of Forensic Science 1993;38(3):691-701
12 Jennifer A., Marshall graves Evolution of the mammalian Y chromosome and sex-determining genes. The Journal of Experimental Zoology 1998;281:472-481   DOI   ScienceOn
13 Hiroki Murakami, Yuji Yamamoto et al. Forensic study of sex determinati-on using PCR on teeth samples. Acta Med Okayama 2000;54(1):21-32
14 Harry Ostrer. Identifying genes for male sex determination in humans. Journal of experimental zoology 2001;290:567-573   DOI   ScienceOn
15 Katsuhiko Omeoe, Akira Endo. Relationship between the monosomy X phenotype and Y-linked ribosomal protein S4(RPS4) in several species of mammals : A molecular evolutionary analysis of RPS4 homologs. Genomics 1996;31:44-50   DOI   ScienceOn
16 Salido, EC., Yedn, PH. The human enamel protein gene amelogenin is expressed from both the X and Y chromosomes. Am. J. Hum. Genet. 1992;50:303-316
17 Ester Margarit, Anna Soler. Molecular, cytogenetic, and clinical characterisation of six XX males including one prenatal diagnosis. J. Med Genet. 1998;35:727-730   DOI
18 R.L. Souhami, J. Moxham. Textbook of Medicine 1990;720
19 Krogeman, WM.. The human skeleton in forensic medicine. Springfield 1978;11-17
20 Andrew H. Sinclair. Human sex determination. The Journal of Experime-ntal Zoology 1998;281:501-505   DOI   ScienceOn
21 Harvey, W. Dental identification and forensic odontology. Henry Kimpton publishers 1976;36-37, 67-68
22 Vergnaud, G., Kaplan, L. et al. Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes. British Medical Journal 1984;289:73-76   DOI
23 Erlich, HA., Gelfand, D., Sninsky, JJ. Recent advances in the polymerase chain reaction. Nature 1991;252:1643-1651
24 Goodfellw, PN. Mapping the Y chromosome. Development 1987;supplement 39
25 Hur W., Yoon CL. Comparison of 4 methods of DNA extraction for sex determination and D1S80 locus detection in teeth. Korean Journal of Oral Medicine 1995;20(2):497-512   과학기술학회마을
26 Andrew R. Zinn, Raaji K. Alagappan et al. Structure and function of ribosomal protein S4 genes on the human and mouse sex chromosomes. Molecular and Cellular Biology 1994;2485-2492
27 Witt M., Erickson RP. A rapid method for detection of Y-chromosomal DNA from dried blood specimens by the polymerase chain reaction. Hum Genet. 1989;82:271-274   DOI
28 David Miller. Sex determination : insights from the human and animal models suggest that the mammalian Y chromosome is uniquely specialised for the male's benefit. Jmhg. 2004;1:170-181   DOI   ScienceOn