• Title/Summary/Keyword: Pulmuone

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Research trends and development direction in cell-based seafood (배양수산물의 연구현황 및 발전방향)

  • Jung, Jin-Kyoung;Park, Young-Min;Kim, Sang-Gu;Lee, Sang-Yoon
    • Food Science and Industry
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    • v.55 no.1
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    • pp.95-104
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    • 2022
  • Climate change due to global warming is affecting the global environment. The fishery industry is highly dependent on the natural environment, so the fishery industry is increasingly volatile due to the rapidly changing climate change. At the same time, consumers' anxiety due to marine pollution such as microplastics, radioactivity, and heavy metals are increasing. Accordingly, cell-based seafood are being proposed as an alternative for the sustainable use of seafood resources. Cell-based seafood manufactured through a process of differentiation and proliferation using cells isolated from actual seafood and cell, scaffold, media, and bioreactor technologies are organically connected to each other. This review summarizes the latest research status of cell-based seafood. Based on this, suggests a development direction for cell-based seafood to properly settle in the future market.

Isolation, Characterization, and Control of Pseudomonas kribbensis and Pantoea vagans that cause Soft-rot Disease Isolated from Chinese Cabbages

  • Lee, Kang Wook;Kim, Geun Su;Kim, Jeong A;Kwon, Do Young;Lee, Jin Ju;Kim, Il Chul;Kim, Sang Gu;Kim, Tae Seok;Lee, Sang Yun
    • Journal of Food Hygiene and Safety
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    • v.37 no.2
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    • pp.55-62
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    • 2022
  • The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

Phytochemical Constituents of Acanthopanax senticosus (Rupr. & Maxim.) Harms Stem

  • Ryu, Ji-Young;Son, Dong-Wook;Kang, Jung-Il;Lee, Sang-Yun;Kim, Hyun-Su;Shin, Kuk-Hyun;Lee, Sang-Hyun
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.4
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    • pp.306-310
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    • 2003
  • Five constituents were isolated from the stem of Acanthopanax senticosus. Their structures were elucidated as (-)-sesamin (1), iso-fraxidin (2), 5-hydroxymethylfurfural (3), syringin (4) and acanthoside D (5) by spectral analysis. Among these compounds, 5-hydroxymethylfurfural (3) was isolated for the first time from this plant.

Isolation and Characterization of Kimchi Starters Leuconostoc mesenteroides PBio03 and Leuconostoc mesenteroides PBio104 for Manufacture of Commercial Kimchi

  • Lee, Kang Wook;Kim, Geun Su;Baek, A Hyong;Hwang, Hyun Sun;Kwon, Do Young;Kim, Sang Gu;Lee, Sang Yun
    • Journal of Microbiology and Biotechnology
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    • v.30 no.7
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    • pp.1060-1066
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    • 2020
  • This study was focused on developing and obtaining a kimchi starter for use in commercial kimchi production. Kimchi varieties made with selected starters are of high quality, have high levels of mannitol, and extended shelf life. The starters were screened for properties such as mannitol production, low gas/acid production, and acid resistance. Finally, kimchi fermentation testing was performed using selected LAB starters. Kimchi samples were prepared with lactic acid bacteria (LAB) starters, including Leuconostoc mesenteroides PBio03 and Leuconostoc mesenteroides PBio104. The LAB starters are isolated from kimchi and can grow under pH 3.0 and low temperature conditions of 5℃. Four kimchi samples were fermented and stored for 28 days at 5℃. The kimchi samples made with starters (PBio03 and PBio104) had better quality (production of mannitol and maintenance of heterofermentative LAB dominance) than the non-starter kimchi samples. In the starter kimchi, Leu. mesenteroides was the dominant LAB, comprising 80% and 70% of total LAB counts at 7 and 21 days, respectively. Mannitol content of the kimchi with Leu. mesenteroides PBio03 was 1,423 ± 19.1 mg/100 g at 28 days, which was higher than that of the non-starter kimchi sample (1,027 ± 12.2 mg/100 g). These results show the possibility of producing kimchi with improved qualities using Leu. mesenteroides PBio03 and PBio104 as starters.

Effect and Safety of a Commercial diet-food(Pulmuone $Diet^{(R)}$) in normal health obese females (건강 비만성인에 있어서의 시판 다이어트 제품의 다이어트효과와 안전성에 대한 연구)

  • Shin, Hyun-Taek;Kweon, Hyuk-Myung;Kim, Soo-Bin;Kwang, Sin-Uk;Yu, Ik-Hyun
    • Korean Journal of Clinical Pharmacy
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    • v.5 no.2
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    • pp.51-60
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    • 1995
  • Obesity is defined as excessive storage of energy in the form of fat resulting in adverse effect on healthy and longevity. Some data have shown increased incidence of hypertension, diabetes, hypercholesterolemia in overweight individuals and other disorders such as joint disease, cancer have been also associated with obesity. Recently, various commercial diet programs have been introduced for weight reduction without scientific evaluation for clinical effect and safety. The diet program must be based on a sound and scientific rationale and also be safe and nutritionally adequate. Pulmuone $diet^{(R)}$ is a calorie-restricted commercial diet-food, which contains adequate nutritients. The efficacy and safety of Pulmuone diet were evaluated in 28 normal healthy obese females in a prospective study. Each volunteer was placed in 3-week diet program. To observe weight reduction effect, body weight, TSF and AMC were measured every week. To account for safety of this program, blood chemistry test, blood pressure and side effects were monitored every week. Mean body weight was reduced by 3.24kg following diet program, which is statistically significant(P < 0.05) and this weight reduction was related to reduction in fat weight and not muscle weight as shown by the results of TSF and AMC Blood chemistry was not influenced by this program, but blood pressure was significantly reduced following this program. Thought diet program was generally well tolerated in most volunteers, the most frequent side effects are constipation, dizzness and headache. In conclusion, Pulmuone $diet^{(R)}$ program seems to be safe and effective for weight reduction in normal healthy obese females.

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Passion + Innovation + Marketing = A Successful New Market Development 『A Case of Pulmuone Fresh Ramen, 'Jayeonun Masitda'』 (열정 + 혁신 + 마케팅 = 신시장 창출 『풀무원 '자연은 맛있다'의 생라면 시장 개척 사례』)

  • Chu, Kyounghee;Lee, Doo-Hee;Park, Seong Yeon;Yoo, Shijin
    • Asia Marketing Journal
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    • v.13 no.3
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    • pp.233-248
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    • 2011
  • This case illustrates a story of passionate and innovative new market development by Pulmuone, a fresh food provider in Korea. The company has been successfully developing a new market by introducing a (non-frying) fresh ramen, 'Jayeonun Masitda - The Nature Is Tasty' in the packaged ramen market dominated by fried ones. In this case, a detailed new market development process by Pulmuone will be investigated including; company overview, a new product development process, marketing strategy formulation, marketing mix implementation, market performance, and future directions. Pulmuone has been making efforts to create a new product category by marketing non-frying ramens since 1995, but with a modest success. In 2011, Pulmuone finally succeeded to develop an innovative product, 'Jayeonun Masitda' that brought more health and nutrition conscious consumers' attention in the ramen market. The company intended to change the current competitive structure in the ramen market, i.e., from the strength of taste and the amount of ingredient to fried/non-fried and the freshness of ingredient. By this new positioning, Pulmuone aimed to reshaping the ramen market into competition between healthy and unhealthy ramens. Pulmuone has been successful in developing a new market. Sales revenue of 'Jayeonun Masitda' has been continuously increasing, and customers are found to be highly satisfied with the product resulting in a high repeat purchase rate. The company's successful new market development can be attributed to a faithful new product development process, innovative technology, an appropriate positioning strategy, and consistent marketing communication. In addition, Pulmuone's eco-friendly corporate image and the organization's passion to grow are also important factors for success of this new market development.

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Genetic characterization of Phellinus baumii PMO-P4 by analyzing restriction fragment length polymorphisms of nuclear ribosomal DNA internal transcribed spacers (ITS) (Ribosomal DNA의 ITS부위에 대한 RFLP 분석에 의한 Phellinus baumii PMO-P4의 유전학적 특성)

  • Chang, Yun-Hee;Kim, Tae-Rack;Kim, Hyun-Su;Yeo, Ik-Hyun;Lee, Sang-Youn;Ha, Hyo-Cheol
    • Journal of Mushroom
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    • v.4 no.2
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    • pp.43-47
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    • 2006
  • PMO-P4, being cultivated as "Sanghwang" in Korea, was proved to be P. baumii based on ITS (internal transcribed spacer) sequencing and RFLP (Restriction Fragment Length Polymorphism) patterns along with some Phellinus species including P. linteus. The similaraty of ITS sequencing between PMO-P4 and other Phellinus species was given the range of 48.6%~72.2%, showing the highest homology from P. linteus and the lowest from P. gilvus.

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Analysis of Flavonoids in Concentrated Pomegranate Extracts by HPLC with Diode Array Detection

  • Lee, Jeong-Hwan;Kim, Seung-Deok;Lee, Ja-Young;Kim, Kyung-Nam;Kim, Hyun-Su
    • Food Science and Biotechnology
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    • v.14 no.1
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    • pp.171-174
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    • 2005
  • Three flavonoid compounds - quercetin, luteolin, and kaempferol - were analyzed from two commercially available concentrated pomegranate extracts produced in Turkey and Italy, respectively. The samples were freeze-dried and hydrolyzed by 0.4 M hydrochloric acid in 50% ethanol at $80^{\circ}C$. HPLC (high-performance liquid chromatography) with DAD (diode array detection) at a wavelength of 260 nm was used for the detection of the three flavonoids. The detection limits of the three compounds were in hundreds of picograms and the signal-to-noise ratio ranged from 4 to 5: quercetin: >308 pg, s/n=4.0; luteolin: >262 pg, s/n=4.5; kaempferol: >688 pg, s/n=5.0. Quercetin, but not luteolin and kaempferol, was detected in the both pomegranate extracts. The concentrations of quercetin were $49.7\;{\mu}g/g$ and $27.7\;{\mu}g/g$ in the two pomegranate extracts made in Turkey and Italy, respectively.