• 제목/요약/키워드: Psoralea corylifolia

검색결과 36건 처리시간 0.057초

보골지 및 염초보골지의 proteasome 저해 작용 (Protesome Inhibition Activity of Psoraleae Semen and Processed Psoraleae Semen)

  • 심상희
    • 생약학회지
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    • 제39권1호
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    • pp.56-59
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    • 2008
  • Ubiquitin-proteasome proteolytic system plays an important role in selective protein degradation and regulates cellular events including apoptosis. Cancer cells have been shown to be more sensitive to the proapoptotic effects of proteasome inhibition than normal cells. Thus, proteasome inhibitor can be potential anticancer agent. Since the MeOH extracts of psoraleae semen and processed psoraleae semen showed potent proteasome inhibition activity, the fractions of the extracts were evaluated on the activity to screen the proteasome inhibitors. The $CHCl_3$ fr. of the processed psoraleae semen showed the most potent activity, of which chemical investigation led to two coumarins, psoralen and isopsoralen. Their structures were determined by spectroscopic methods such as $^1H-NMR$ and EIMS spectra.

부정방암탕(扶正防癌湯) 구성(構成) 약물(藥物)의 암전이(癌轉移) 억제(抑制)에 대한 연구(硏究) (Anti-metastastic Effects of ingredients of Fuzhengfangaitang)

  • 박준혁;유영법;심범상;최승훈;안규석
    • 대한한방종양학회지
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    • 제7권1호
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    • pp.39-60
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    • 2001
  • Fuzhengfangaitang is a prescript for inhibiting recurrence and metastasis of cancer. We had examined the anti-metatstastic effect of Fuzhengfangaitang. Furthermore, we performed the following experiments with ingredients of Fuzhengfangaitang. The purpose of this thesis is to study what ingredients of Fuzhengfangaitang have more valuable anti-cancer effects. And the results are listed below: 1. Cell Viability assay At the dose of 400$\mu$g/ml, most ingredients of Fuzhengfangaitang depressed viability of ECV-304. And especially, Scutellaria barbata D. DON$50{\mu}g/ml$ : 53.118%, $100{\mu}g/ml$: 49.092%, $200{\mu}g/ml$ : 43.765%, $400{\mu}g/ml$ : 12.747%), Polygonum bistorta L.($50{\mu}g/ml$ : 45.554%, $100{\mu}g/ml$ : 45.554%, $200{\mu}g/ml$ : 0.0%, $400{\mu}g/ml$ : 0.0%) and Psoralea corylifolia L.($50{\mu}g/ml$ : 86.591%, $100{\mu}g/ml$ : 81.307%, $200{\mu}g/ml$ : 24.801%, $400{\mu}g/ml$ : 3.111%) highly depressed cell viability more than the other ingredients. (${\alpha}$<0.05) 2. Cell Proliferation assay Proliferation assay with ingredients of Fuzhengfangaitang on ECV-304 showed that Crataegus pinnatifuda BGE ($50{\mu}g/ml$: 63.276%, $100{\mu}g/ml$ : 64.092%, $200{\mu}g/ml$ : 68.966% $400{\mu}g/ml$ : 38.517%, ED50=$296.974{\mu}g/ml$), Polygonum bistorta L.($50{\mu}g/ml$ : 83.981%, $100{\mu}g/ml$ : 86.997%, $200{\mu}g/ml$ : 58.780%, $400{\mu}g/ml$ : 26.408%), ED50=$266.725{\mu}g/ml$) and Psoralea corylifolia L.($50{\mu}g/ml$ : 103.037%, $100{\mu}g/ml$ : 82.529%, $200{\mu}g/ml$ : 2.829%, $400{\mu}g/ml$ : 0.998%), ED50=$177.369{\mu}g/ml$) depressed cell proliferation more than the other ingredients. 3. Tube Formation assay Compared with the control group, most ingredients of Fuzhengfangaitang did not remarkably inhibited the Tube Formation assay of ECV-304 at the dose of $100{\mu}g/ml$. But, Polygonum bistorta L. highly inhibited the tube formation of ECV -304 at the lower dose of $50{\mu}g/ml$. 4. Rat Aortic Ring assay In comparison with the control group, Scutellaria barbata D. DON., root of Polygonum bistorta L. and Psoralea corylifolia L. restricted the angiogenesis of the rat aortic ring at the dose of $100{\mu}g/ml$. And the other ingredients of Fuzhengfangaitang did not restricted the angiogenesis of the rat aortic ring at that dose. Especially, Polygonum bistorta L. highly inhibited the angiogenesis of the rat aortic ring at the lower dose of $50{\mu}g/ml$. From our research, the anti-angiogenic effects of the ingredients of Fuzhengfangaitang was proven. Moreover, it will be helpful for designing more effective prescription for anti angiogenesis.

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천연 약용식물 추출물의 여드름 원인균에 대한 항균 및 항염증 효과 (Antibacterial and Anti-inflammatory Effects of Medicinal Plants Against Acne-inducing Bacteria)

  • 이응지;배성윤;남궁우;이용화
    • 대한화장품학회지
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    • 제36권1호
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    • pp.57-63
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    • 2010
  • 여드름 완화 효과를 갖는 천연 약용식물 추출물을 개발하기 위하여 여드름 원인균에 대한 항균 활성 및 항염증 효과를 다양한 추출물을 대상으로 분석하였다. 검색 결과 얻어진 후보 소재들 중, 비교적 높은 항균 활성을 갖는 보골지, 당후박과 항염증 효과를 갖는 목향, 구절초 추출물을 대상으로 선정하였고 이후의 실험을 진행하였다. 보골지 (Psoralea corylifolia L., AC-1), 당후박(Magnoliae officinalis. AC-2) 목향(Inula helenium L., ACF-1), 구절초(Chrysanthemum zawadskii var. latilobum, ACF-2) 추출물 및 복합소재 (AC)는 $50{\mu}g/mL$ 이하에서 세포 독성이 없는 것으로 확인되었다. 보골지, 당후박 추출물 및 복합소재의 경우 여드름 유발 원인균인 Propionibacterium acnes (P. acnes)에 대해 양성대조군으로 사용한 10 % salicylic acid 대비 각각 약 2.8배, 2.5배, 3.2배의 높은 항균 효과를 보였다. 또한 Staphylococcus aureus (S. aureus) 에 대해서도 당후박 추출물과 복합소재의 경우 양성 대조군으로 사용한 10 % Methylparaben 대비 각각 약 1.4배, 1.5배 높은 항균 효과를 보였다. 목향 구절초 추출물 및 복합소재의 경우 열에 의해 사멸시킨 P. acnes에 의해 활성화된 THP-1 세포의 IL-8 발현을 $50{\mu}g/mL$ 농도에서 각각 27%. 38%, 44% 저해하였고 TNF-$\alpha$ 발현을 $50{\mu}g/mL$ 농도에서 각각 90%, 88%, 90%로 크게 저해하였다. 이러한 항균, 항염증 활성을 보이는 보골지, 당후박, 목향, 구절초 추출물의 복합 소재를 이용하여 여드름 완화 효과를 갖는 화장품으로의 개발이 가능할 것으로 보인다.

보골지, 오미자, 계혈등 추출물의 항균활성 (Antimicrobial Activity of Psoralea corylifolia, Schisandra chinensis, and Spatholobus suberectus Extracts)

  • 최은정;장성란;강옥주;방우석
    • 한국식품과학회지
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    • 제45권4호
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    • pp.495-500
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    • 2013
  • 약용식물 3종에 대한 항균활성 결과, 보골지와 오미자 분획층에서 계혈등 분획층보다 높게 나타났다. 계혈등과 보골지 n-hexane 분획층에서 그람 음성균보다 그람 양성균에 대한 항균활성이 유의적으로 높게 나타났으며(p<0.05), 항균활성은 농도가 증가함에 따라 증가하였다. 계혈등 n-hexane 분획층의 L. monocytogenes에 대한 MIC는 7.765 (mg/mL), 보골지 n-hexane 분획층의 B. cereus, L. monocytogenes, E. coli 균에 대한 MIC는 2.03 (mg/mL), 오미자 분획층의 (L. monocytogenes), E. coli 균에 대한 MIC는 8.5 (mg/mL)로 나타났으며, 오미자 n-hexane 분획층의 MIC가 모든 균주에서 가장 낮게 나타났다. 약용식물 추출물의 항균활성에 대한 열 안정성 실험결과, L. monocytogens에 대한 계혈등 n-hexane 분획층을 제외하고는 $100^{\circ}C$에서 10분간의 열처리에도 항균 활성의 변화가 없었다. 본 연구 결과 보골지와 오미자 n-hexane 분획층에서 비교적 높은 항균활성을 나타냈다. 이러한, 약용 식물은 다양한 생리활성 및 기능성 성분을 포함하고 있어 천연 항균제의 유용한 자원으로 이용 될 수 있으며, 식품산업 발달에 기여 할 수 있을 것으로 사료된다.

동의보감(東醫寶鑑) 요문(腰門)의 요통처방(腰痛處方)에 대(對)한 분석(分析) (The Analysis of Prescription Used for Low Back Pain in the Yomun(腰門) Chapter of 《Donguibogam(東醫寶鑑)》)

  • 안중혁;이명종
    • 한방재활의학과학회지
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    • 제15권1호
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    • pp.77-87
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    • 2005
  • Objectives: This analysis of prescription used for low back pain in the Yomun(腰門) chapter of ${\ll}$Donguibogam(東醫寶鑑); The Precious Mirror of Oriental Medicine${\gg}$ is designed to be helpful to practical use of clinics. Methods : Proscriptions used for low back pain in the Yomun(腰門) chapter of ${\ll}$Donguibogam(東醫寶鑑)${\gg}$ were classified and analyzed according to the frequency of proscriptions and the characteristics of each herbs in proscriptions(efficacy, used frequency, related organs etc.) Results and conclusions : After analysis, we obtained the following results : 1. The causes of low back pain are mainly eohyeol(瘀血), yangheo(陽虛), punghanseub(風寒濕). In care of low back pain, I suppose more efficiency that if Angelica gigas NAKAI(當歸) Cnidium officinale MAKINO(三芎) Prunus persica BATSCH(桃仁) is added when the cause is eohyeol(瘀血), or if Psoralea corylifolia L.(破古紙), Cinnamomum cassia PRESL(肉桂), Foeniculum vulare MILL(茴香), Eucommia ulmoides OLIV.(杜冲), Citrus unshiu MARKOVICH(陳皮) are added when the cause is yangheo(陽虛), or if Phellodendron amurense RUPR.(黃柏), Notopterygium incisum TING(羌活), Atractylodes Japonica KOIDZ.(蒼朮) are added when the cause is punghanseub(風寒濕).

Neuroprotective Effects of Medicinal Herbs in the Transient Focal Ischemia

  • Oh, Jin-Kyung;Jung, Ji-Wook;Ahn, Nam-Yoon;Oh, Hye-Rim;Ryu, Jong-Hoon
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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    • pp.64-64
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    • 2003
  • It is thought that highly reactive oxygen radicals generated at the ischemia-reperfusion in case of strokes play an important role in damaging the brain. We examined the neuroprotective effects from the several medicinal herbs in the transient ischemic rat model and compared their effects with the free radical scavenging activities. Transient ischemia was induced by intraluminal occusion of the right middle cerebral artety for 120 min and reperfusion was continued for 22 h in rats. The free radical scavenging properties of medicinal herbs were examined in vitro by determination of the interaction with the 1,l-diphenyl-2-picrylhydrazyl (DPPH) stable free radical. Aqueous extracts of 11 medicinal herbs (200 mg/kg) were orally administered, promptly prior to reperfusion and 2 h after reperfusion. Total infarction volume in the ipsilateral hemisphere of ischemia reperfusion rats was significantly lowered by the treatment of 7 medicinal herbs (Sophora flavescens, Lycopus lucidus, Sanguisorba officinalis, Caesalpinia sappan, Albizia julibrissin, Rubia akane, Psoralea corylifolia, Prunella vulgaris). However, all of these medicinal herbs did not show antioxidative activities. These results suggest that neuroprotective effects of several drugs are not always correlated with their antioxidative properties.

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Screening for Anti-diabetic Effects of Prescribed Korean Traditional Medicines

  • Shin, Seon Mi;Jeong, Yong Joon;Park, Dae Won;Ko, Heung;Kim, Gi Tae;Kim, Ee-Hwa;Kim, Taeseong;Sohn, Eun-Hwa;Kwon, Jung-Eun;Koo, Hyun Jung;Kang, Se Chan
    • 한국자원식물학회지
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    • 제25권6호
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    • pp.670-681
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    • 2012
  • Herb extracts commercially used in Korea were screened for PPAR-${\gamma}$ agonist test and ${\alpha}$-glucosidase inhibition assay. Total 16 herb plants had a PPAR-${\gamma}$ agonist activity. Specially, Alisma orientale Juz (108.41%), Ephedra sinica (98.22%), Sasa japonica Makino var. purpurascens Nakai (140.68%), Astragalus membranaceus Bunge (106.79%) and Cnidium officinale Makino (113.00%) showed high PPAR-${\gamma}$ agonist activity rate compared with rosiglitazone's (167.46%). And Cornus officinalis S. et Z. (90.3%), Cinnamomum cassia Blume (89.2%), Psoralea corylifolia L. (89.8%), Paeonia japonica (Makino) Miyabe (92.4%) and Paeonia suffruticosa Andr (93.2%), showed high ${\alpha}$-glucosidase inhibition rates. These results support previous reports of the efficacy of Oriental medicinal plants used for diabetes mellitus.

보골지(補骨脂)가 남성 생식세포 GC-1의 항산화에 미치는 영향 (Antioxidant Effects of Psoraleae Fructus in GC-1 Cells)

  • 오명숙;김도림;김소연;장문석;박성규
    • 동의생리병리학회지
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    • 제19권1호
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    • pp.81-86
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    • 2005
  • The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Psoraleae fructus. The extract was studied for dipheny-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being 0.427 mg/mL. The extract was dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (67.7 %) was blocked by the extract concentration- dependently. Furthermore, the extract also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Psoraleae fructus has potent antioxidant activity.