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Antioxidant Effects of Psoraleae Fructus in GC-1 Cells  

Oh, Myung-Sook (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, Do-Rim (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Kim, So-Yeon (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Chang, Mun-Seog (Department of Medicine, Division of Newborn Medicine, Children's Hospital and Harvard Medical School)
Park, Seong-Kyu (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.19, no.1, 2005 , pp. 81-86 More about this Journal
Abstract
The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Psoraleae fructus. The extract was studied for dipheny-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being 0.427 mg/mL. The extract was dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (67.7 %) was blocked by the extract concentration- dependently. Furthermore, the extract also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Psoraleae fructus has potent antioxidant activity.
Keywords
Psoraleae fructus; Psoralea corylifolia L.; dipheny-picryl-hydrazyl (DPPH); MTT assay; Hydrogen peroxide $(H_2O_2)$; cytotoxicity; Lipid peroxidation (LPO); GC-1 cells;
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