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Association of Succinate Dehydrogenase and Triose Phosphate Isomerase Gene Expression with Intramuscular Fat Content in Loin Muscle of Korean (Hanwoo) Cattle (한우 등심조직 내 succinate dehydrogenase 및 triosephosphate isomerase 발현이 근내 지방함량에 미치는 영향에 관한 연구)

  • Kim, Nam-Kuk;Lee, Seung-Hwan;Lim, Da-Jeong;Yoon, Du-Hak;Lee, Chang-Soo;Kim, Oun-Hyun;Kim, Hyeong-Cheol;Oh, Sung-Jong;Hong, Seong-Koo
    • Journal of Life Science
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    • v.22 no.1
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    • pp.31-35
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    • 2012
  • In a previous study, succinate dehydrogenase (SDH) and triose phosphate isomerase (TPI) were detected by 2D gel electrophoresis as differentially expressed proteins in the longissimus thoracis muscles of cattle aged between 12 and 27 months old. In the present study, we investigated the association of SDH and TPI gene expression with intramuscular fat content in 50 Hanwoo steers. The SDH gene was expressed at a 4 times higher level in the 12 month old group than in the 27 month old group (p<0.01). A regression analysis between gene expression value and intramuscular fat content showed a negative correlation between expression of the SDH gene and intramuscular fat content (p<0.001). In contrast, the expression of the TPI gene showed no significant association with intramuscular fat content. This result suggests that the SDH gene may be a candidate marker gene for intramuscular fat content in the longissimus thoracis of Korean cattle.

Soil Chemical Properties of Major Vegetable Producing Open Fields (주요(主要) 노지채소(露地菜蔬) 주산지(主産地) 토양(土壤)의 화학적(化學的) 특성(特性))

  • Hwang, Ki-Sung;Lee, Sung-Jae;Kwack, Yong-Ho;Kim, Ki-Sun
    • Korean Journal of Soil Science and Fertilizer
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    • v.30 no.2
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    • pp.146-151
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    • 1997
  • To get the basic information for the establishment of the optimum levels of upland soil fertility and fertilizer application, two hundred soil samples were collected from the major vegetable cultivation areas such as Chinese cabbage, reddish, garlic, onion, red pepper, watermelon and potato fields. The soil samples were analyzed for the soil chemical properties and micro elements. Soil pH, organic matter and magnesium contents were lower than the standard level for upland soil improvement, while the phosphate and potassium contents were higher than the standard levels. The organic matter and nitrogen contents were increased in the potato field soils, the available phosphate contents were increased in the red pepper field soils and the exchangeable potassium contents were increased in reddish and red pepper field soils, to compared with the past deta. The contents of micro elements were ranged in 14~282 for Fe, 13~98 for Mn, 0.5~2.8 for Cu and 0.6~5.0 mg/kg for Zn respectively, and they were in order of Fe>Mn>Zn>Cu.

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An Experimental Study on Debris Reduction System for Culvert (암거의 유송잡물 저감시설에 관한 실험 연구)

  • Kim, Sung-Joong;Kang, Joon-Gu;Kim, Jong-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.10
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    • pp.696-706
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    • 2017
  • The purpose of this study was to verify experimentally debris reduction facilities for culverts installed in small rivers. A culvert is defined as a structure laid under a road or a railroad that passes through an inner urban area or downtown area to make an artificial canal. Culverts are generally categorized into road culverts or waterway culverts, among which the latter are artificial structures designed to discharge running water into a river. At the time of floods, the structural safety of waterway culverts can be undermined by the accumulation of debris, such as soil, boughs and weeds, and they may be at risk of overflowing due to blockages. Debris reduction facilities are necessary to prevent such damage. In this study, the effects of the three existing types of debris reduction facilities were examined through hydraulic experiments. The results of the experiments showed that vertical separation to divert debris reduced the accumulation rate by 27.65 to 31.39 percent. The two types of screen designed to block and divert debris, respectively, were found to have excellent debris blocking abilities. However, when the effects of the rising water level are considered simultaneously, the screen to divert debris was found to show superior effects. The screen to block debris can be considered to have excellent debris blocking ability, but requires the continuous collection of the debris, due to the high risk of rising water levels caused by its accumulation.

Physicochemical characteristics and fatty acid profiles of muscle tissues from Hanwoo steers fed a total mixed ration supplied with medicinal plant by-products

  • Lee, Shin Ja;Kim, Do Hyung;Yang, Han Sul;Nam, Ki Chang;Ahn, Seung Kyu;Park, Sung Kwon;Choi, Chang Weon;Lee, Sung Sill
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.10
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    • pp.1388-1395
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    • 2017
  • Objective: Using medicinal plant by-products (MPBP) as feed additives may be an eco-friendly option as substitutes for feedstuffs and may assist in reducing the improper disposal of MPBP. Therefore, this study was conducted to evaluate the influences of MPBP on the meat quality of Hanwoo steers fed a total mixed ration (TMR). Methods: Twenty seven steers (body weight = $573{\pm}57kg$) were randomly divided into three treatments with a control group and two tested groups as follows: control, 1,000 g/kg TMR; treatment 1 (MPBP30), 970 g/kg TMR and 30 g/kg MPBP; treatment 2 (MPBP50), 950 g/kg TMR and 50 g/kg MPBP. Results: Average daily gain, feed conversion ratio and the Commission Internationale de l'Eclairage $L^*$ of muscle were improved (p<0.05, respectively) by MPBP30. Stearic acid ($C_{18:0}$) was decreased (linear effect, p = 0.012), while oleic acid ($C_{18:1}$) was increased (linear effect, p = 0.055) by MPBP level. Saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) were decreased for MPBP50 while unsaturated fatty acid (USFA) and monounsaturated fatty acid (MUFA) were increased for MPBP 50. USFA and SFA ratio was increased for MPBP50 as well. Conclusion: These results indicated that MPBP supplementation in Hanwoo steers fed a TMR increased feed efficiency and meat color (lightness) with altering fatty acid proportions. Therefore, MPBP may be successfully used in ruminant feeding.

The Understanding of the Forest Campers' Attitudes and Their Self-Actualization in Relation to Quality of Forest Resources (야영객(野營客)의 삼림자원(森林資源)에 대한 심리자세(心理姿勢)와 자아실현(自我實現))

  • Shin, Won Sop
    • Journal of Korean Society of Forest Science
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    • v.82 no.2
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    • pp.107-121
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    • 1993
  • This study examines whether or not forest campers' self-actualization, that is the tendency for a person to become actualized in what he or she perceived to be his or her own potential, and their attitudes toward nature were related to quality of forest areas where they visited. People were sampled in three Ontario Provincial Parks in Canada from September of 1988 to August of 1989. From a total of 138,317 campers, 540 were sampled randomly to collect data. People sampled ranged in age from 15 to 75 years with a mean of slightly over 37. They were highly educated. A majority of them resided in urban or suburban areas. Campers' level of self-actualization and their nature attitudes were highly correlated with forest area quality in both perceived or subjective and objective measures. Their self-actualization scores were also highly correlated with their attitudes toward nature. However, there was no significant relationships among campers' self-actualization, attitudes toward nature, and quality of forests where they camped when they were treated simultaneously.

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Crop Performance and Soil Chemical Properties Affected by Amounts of Normal Water after Simulated Acid Rain (인공산성(人工酸性)비 후(後) 일반(一般)비 처리량(處理量)에 따른 작물생육(作物生育)과 토양(土壤)의 화학성(化學性))

  • Kim, Bok-Jin;Park, Suen-Do;Lee, Suk-Soon
    • Korean Journal of Environmental Agriculture
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    • v.15 no.3
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    • pp.341-347
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    • 1996
  • A green-house experiment was conducted to investigate the growth of Chinese cabbage, radish, and soybean and changes in soil chemical properties after application of 5 and 10mm of pH 2.7 simulated acid rain(SAR) followed by 0, 5, and 10mm of pH 6.0 normal water at the three-day intervals 20 times for Chinese cabbage and radish and 42 times for soybean. The results obtained are summarized as follows: 1. Visual damages by SAR were white-yellow leaf spots, dark brown or light green leaf color, and wrinkled leaf margins in all crops. The degree of visual damages was severer at 10mm than at 5mm SAR and it was reduced as the amounts of normal water increased after SAR application. 2. Chlorophyll content was higher at 10mm than at 5mm SAR application and increased as the amounts of normal water increased after SAR application in all crops. 3. Fresh weights of Chinese cabbage heads and radish roots and grain yield of soybean were higher at 10mm than at 5mm SAR, while they increased as the amounts of normal water increased at the same SAR level. 4. Changes in the mineral contents of plants were not consistent, while S content in radish and soybean increased as the amounts of SAR increased. 5. SAR lowered soil pH, while it increased soil N and S contents. Contents of soil organic matter, P, and exchangeable Ca, Mg, and K were not affected by the amounts of SAR.

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Effects of Dietary Glycine Betaine on the Growth Performance in Pigs (Glycine betaine 첨가가 돼지의 생산성에 미치는 영향)

  • Kwak, S.C.;Kim, J.H.;Ha, Y.J.;Lee, J.I.;Lee, J.R.;Jung, J.D.;Lee, J.D.;Park, G.B.;Ko, Y.D.
    • Journal of Animal Science and Technology
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    • v.47 no.2
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    • pp.205-220
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    • 2005
  • This study was conducted to investigate the effect of the addition of glycine betaine to the diet on growth performance in pigs. A total of 400 pigs were divided into 4 feeding stages(Growing I: 23.10 $\pm$ 1.43, Growing II: 37.69$\pm$ 1.62, Finishing I: 66.51 $\pm$3.44 and Finishing II: 90.42$\pm$ 2.17 kg of initial body weight) then each feeding stage was divided into 4 treatment groups(Control: 0 0/0, Tl : 0.2 0/0, T2: 0.4% and T3 : 0.6 % of glycine betaine, respectively). The average daily gain and feed efficiency of T2 and T3 were significantly increased(p< 0.05) by dietary glycine betaine in stage I, 2 and 3. This result indicates that dietary glycine betaine could influence the pig growth performance. In feeding stage 4, the average daily gain and feed efficiency were significantly increased in 0.4% glycine betaine feeding group compared with other dietary groups(p < 0.05). Results suggest that feeding the pigs 0.4 % glycine betaine could be the most efficient dietary level. Crude protein, ether extract and crude ash digestibilities of 0.4% glycine betaine fed group were significantly increased compared with those of control group(p < 0.05). However, no significant difference was found in nutrient digestibilities among glycine betaine fed groups. Apparent faecal amino acid digestibilities of 0.4% glycine betaine fed group were more significantly higher than that of control group. The 0.4% glycine betaine fed group was significantly increased in apparent faecal amino acid digestibility compared with those of other glycine betaine fed group. No significant difference was shown in amount of microflora population between control and glycine betaine fed groups. Ammonia and hydrogen sulfide gas emission were significantly decreased in 0.4% glycine betaine feeding group compared with other dietary groups(p < 0.05).

Muscle tissue Distribution Level of Ampicillin in Olive flounder(Paralichthys olivaceus), Rockfish(Sebastes schlegeli), and Red sea bream(Pagrus major) following oral administration (Ampicillin의 경구투여에 따른 양식 어류(넙치, 조피볼락, 참돔)의 근육조직내 잔류량의 변화)

  • Cho Yoon-Hee;Jung Won-Chul;Shin Yong-Woon;Kim Kyoung-Won;Ha Ji-Young;Heo Sung-Hyek;Kim Eui-Gyung;Chung Hee-Sik;Kang Seok-Joong;Choi Yu-Jeong;Kim Suk;Lee Hu-Jang
    • Journal of Veterinary Clinics
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    • v.23 no.2
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    • pp.164-168
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    • 2006
  • The residue depletion of ampicillin was investigated in the olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major) after 5 days treatment with medicated feed at a dose of 100 mg/kg bw/day. Fishes were sampled for muscle on 1st, 2nd, 3rd, and 4th day after treatment. Ampicillin concentrations were determined by high performance liquid chromatography after SPE column extraction. The recovery rates of ampicillin in muscle samples ranged 94-98% and 83-88% for the concentration of 0.05 mg/kg and 0.1 mg/kg, respectively. Ampicillin concentrations detected on 1st day after treatment were 0.143, 0.138, and 0.187 mg/kg in the muscle of olive flounder, rockfish, and red sea bream, respectively. After a withdrawal of 3 days, muscle concentrations were 0.016, 0.012, and 0.021 mg/kg in the olive flounder, rockfish, and red sea bream, respectively. Ampicillin was not detectable in muscle samples on 4 days following withdrawal of the medicated feed. From results of the present study, a withdrawal period of ampicillin is proposed on 5 days after 5 days treatment with medicated feed at a dose of 100 mg/kg bw/day to avoid the presence of excessive residues of the edible muscles of olive flounder, rockfish, and red sea bream.

Effects of Different Silicate Fertilizers on Rice Plant (벼에 대(對)한 규산질비료(珪酸質肥料) 비종별(肥種別) 시용효과(施用效果))

  • Lee, Ki-Sang;Ha, Ho-Sung;Ahn, Sang-Bae;Huh, Beom-Lyang
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.1
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    • pp.32-37
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    • 1986
  • Field experiments were carried out to investigate the effect of wollastonite and iron refinery slag on the paddy rice yield insandyloam soil in which available silica level is 56 ppm. The results are as follows: 1. Application of wollastonite and iron refinery slag reduced the tillering and the height of rice plant in the early growth stage, while promoted those in the late growth stage. 2. As the same amounts of wollastonite and iron refinery slag were applied, the effectiveness on yield increase was same between the two silicate fertilizers. 3. The recovery ratio as measured by absorbed $SiO_2$ on rice plant over applied $SiO_2$, was greater in wollastonite than in iron refinery slag. 4. The increasing of the ratio of $SiO_2$ over N absorbed in rice plant trends toward decreasing the infection rate of neck blast. 5. The left-over ratio of available $SiO_2$ in soil over applied $SiO_2$, was greater in iron refinery slag than in wollastonite. 6. As measured by silica soluble in 0.5 N-HCl solution, 1 kg application per 10a of both silicate fertilizers increased about 2.3 ppm in soil silica content after harvest, respectively.

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Effects of Fermented Cattle Manure Compost Incoporated Autoclaved Lightweight Concrete (ALC) Waste and Raising Duck in Rice Paddy Field on Rice Yield and Quality. (ALC 축분 발효퇴비 시용 및 논오리 사육이 벼 수량 및 미질에 미치는 영향)

  • Kim, Hyun-Ho;Lee, Joo-Yeol;Park, Ki-sun
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.54-58
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    • 1998
  • This experiment was conducted to find out the effects of fermented compost using cattle manure and Autoclaved Lightweight Concrete(ALC) waste and duck raising in rice growing paddy fields on growth and quality of rice. By application of fermented compost of cattle manure incoporated ALC waste with amount of 2,000kg/10a, rice yield was reduced 2.9%, and 4.1% in 1,000kg/10a of compost with raising ducks plots as compared with application of standard level of chemical fertilizer. The ratio of head rice was slightly high in plots of compost application and white-belly kemeled rice was reduced by application of $1,500{\sim}2000kg/10a$ compost. The ratio of Mg/K was the highest in application of 1,000kg compost with raising duck. Efficiency of weed control by raising duck with free hexbiades was sufficient and their effects showed no difference between raising duck plots and herbidde plot.

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