• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.653-653
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• 2013

In this study, ITO extended gate reduced graphene oxide field effect transistor (rGO FET) was demonstrated as a transducer for a proton sensing application. In this structure, the sensing area is isolated from the active area of the device. Therefore, it is easy to deposit or modify the sensing area without affecting on the device performance. In this case, the ITO extended gate was used as a gate electrode as well as a proton sensing material. The proton sensing properties based on the rGO FET transducer were analyzed. The rGO FET device showed a high stability in the air ambient with a TTC encapsulation layer for months. The device showed an ambipolar characteristic with the Dirac point shift with varying the pH solutions. The sensing characteristics have offered the potential for the ion sensing application.

• Journal of the Korean Solar Energy Society
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• v.34 no.4
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• pp.9-16
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• 2014

A proton exchange membrane is a core component in the proton exchange membrane fuel cell because the role of proton exchange membrane(PEM)is supplying proton conductivity to fuel cell, a gas separator, and insulating between an anode and cathode. Among various role of PEM, supplying proton conductivity is the most important and the proton conductivity is strongly related the structural evolution of PEM by hydration. Thus a lot of studies have done by past few decade based on small angle X-ray scattering and wide angle X-ray scattering for understanding morphological structure of the PEM. Resulting from these studies, several morphological models of hydrated PEM are proposed. Current sensing atomic force microscopy (CSAFM) can map morphology and conductance on the membrane simultaneously. It can be the best tool for studying heterogenous structured materials such as PEM. In this study, the hydration of the membrane is examined by using CSAFM. Conductance and morphological images are simultaneously mapped under different relative humidity. The conductance images, which are mapped from different relative humidity, are analyzed by statistical methode for understanding ionic channel variation in PEM.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.656-658
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• 2005

KAERI (Korea Atomic Energy Research Institute) has been performing the project named PEFP (proton engineering frontier project). PEFP has been performing the project of a high power proton accelerator. Control system for 20 MeV proton accelerating structure has developed. We use the EPICS(Experimental Physics and Industrial Control System) tool kit as a foundation of the control system. EPICS is adopted for control systems which have OPI(OPerator Interface) and IOqlnput Output Controller). We have performed the PEPF control system on SUN workstation host computer. In this paper, we present the vacuum monitor, RFQ, and DTL Turbo pump control system.

• Biomolecules & Therapeutics
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• v.15 no.1
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• pp.52-57
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• 2007

Lysolipids such as lysophosphatidylcholine (LPC), sphingosylphosphorylcholine (S PC), galactosylsphingosine (psychosine) have been matched as ligands for OGR1-subfamily G-protein-coupled receptors (GPCR), consisted of OGR1, GPR4, G2A, and TDAG8. Recently, those members of GPCRS have been reported as proton-sensing GPCRs. We used Jurkat T cells, which express four members of OGR1 subfamily GPCRs endogenously to investigate effects of proton on lysolipid-induced several cellular events. We found no significant effect of proton on the lysolipid-induced $Ca^{2+}$ increase and ROS production in Jurkat T cells. Further investigation is necessary to clarify the relationship of lysolipid and proton on the OGR1-subfamily GPCRs.

• Journal of Electrochemical Science and Technology
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• v.1 no.2
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• pp.121-126
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• 2010

An amperometric ascorbic acid selective sensor utilizing the transfer reaction of proton liberated from the dissociation of ascorbic acid in aqueous solution across an elliptic micro-hole water/organic gel interface is demonstrated. This redox inactive sensing platform offers an alternative way for the detection of ascorbic acid to avoid a fouling effect which is one of the major concerns in redox based sensing systems. The detection principle is simply measuring the current change with respect to the assisted transfer of protons by a proton selective ionophore (e.g., ETH 1778) across the micro-hole interface between the water and the polyvinylchloride-2-nitrophenyloctylether gel phase. The assisted transfer reaction of protons generated from ascorbic acid across the polarized micro-hole interface was first characterized using cyclic voltammetry. An improved sensitivity for the quantitative analysis of ascorbic acid was achieved using differential pulse stripping voltammetry with a linear response ranging from 1 to $100\;{\mu}M$ concentrations of ascorbic acid. As a demonstration, the developed sensor was applied for analyzing the content of vitamin-C in different types of commercial pharmaceutical tablets and syrups, and a satisfactory recovery from these samples were also obtained.

• BMB Reports
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• v.49 no.10
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• pp.542-547
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• 2016

Acid-sensing ion channels (ASICs) are proton-gated cation channels widely expressed in the nervous system. Proton sensing by ASICs has been known to mediate pain, mechanosensation, taste transduction, learning and memory, and fear. In this study, we investigated the differential subcellular localization of ASIC2a and ASIC3 in heterologous expression systems. While ASIC2a targeted the cell surface itself, ASIC3 was mostly accumulated in the ER with partial expression in the plasma membrane. However, when ASIC3 was co-expressed with ASIC2a, its surface expression was markedly increased. By using bimolecular fluorescence complementation (BiFC) assay, we confirmed the heteromeric association between ASIC2a and ASIC3 subunits. In addition, we observed that the ASIC2a-dependent surface trafficking of ASIC3 remarkably enhanced the sustained component of the currents. Our study demonstrates that ASIC2a can increase the membrane conductance sensitivity to protons by facilitating the surface expression of ASIC3 through herteromeric assembly.

• Korean Journal of Remote Sensing
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• v.15 no.4
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• pp.289-295
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• 1999

Space Physics Sensor (SPS) on-board the KOMPSAT-1 consists of the High Energy Particle Detector (HEPD) and the Ionospheric Measurement Sensor (IMS). The HEPD is to characterize the low altitude high energy particle environment and the effects on the microelectronics due to these high energy particles. It is composed of four sensors: Proton and Electron Spectrometer(PES), Linear Energy Transfer Spectrometer (LET), Total Dose Monitor (TDM), and Single Event Monitor (SEM). 35 MeV proton beam from the medical KCCH cyclotron, at Korea Cancer Center Hospital in Seoul, is used to calibrate the PES. Primary proton beam of 35MeV scattered by polypropylene target is converted to various energy protons according to the elastic collision kinematics. In this calibration, the threshold level of the proton in the PES can be determined and the energy ranges of PES channels are also calibrated.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.208-213
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• 1999

Space Physics Sensor (SPS) on-board the KOMPSAT-1 consists of the High Energy Particle Detector (HEPD) and the Ionospheric Measurement Sensor (IMS). The HEPD is to characterize the low altitude high energy particle environment and the effects on the microelectronics due to these high energy Particles. It is composed of four sensors: Proton and Electron Spectrometer(PES), Linear Energy Transfer Spectrometer (LET), Total Dose Monitor (TDM), and Single Event Monitor(SEM). 35MeV proton beam from the medical KCCH cyclotron, at Korea Cancer Center Hospital in Seoul, is used to calibrate the PES. Primary proton beam of 35MeV scattered by polypropylene target is converted to various energy Protons according to the elastic collision kinematics. In this calibration, the threshold level of the proton in the PES can be determined and the energy ranges of PES channels are also calibrated.

• Bulletin of the Korean Chemical Society
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• v.25 no.7
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• pp.997-1002
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• 2004

A hydrogenperoxide sensor containing peroxidase extracted from horseradish was constructed and pH effect on its sensing ability was investigated. Current profiles of the biosensor with pH and the electrophoretic analysis showed that horseradish peroxidase consists of two isozymes. Assuming that it is a hypothetical twoisozyme mixture, the current profiles were deconvoluted into two Gaussians. Application of the new Michaelis-Menten equation connoting pH concept to this system enabled to find all the related dissociation constants of the isozyme-substrates and the isozyme-proton complexes and to determine pHs for the maximal isozyme activities.

• BMB Reports
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• v.46 no.6
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• pp.295-304
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• 2013

Extracellular acidification occurs not only in pathological conditions such as inflammation and brain ischemia, but also in normal physiological conditions such as synaptic transmission. Acid-sensing ion channels (ASICs) can detect a broad range of physiological pH changes during pathological and synaptic cellular activities. ASICs are voltage-independent, proton-gated cation channels widely expressed throughout the central and peripheral nervous system. Activation of ASICs is involved in pain perception, synaptic plasticity, learning and memory, fear, ischemic neuronal injury, seizure termination, neuronal degeneration, and mechanosensation. Therefore, ASICs emerge as potential therapeutic targets for manipulating pain and neurological diseases. The activity of these channels can be regulated by many factors such as lactate, $Zn^{2+}$, and Phe-Met-Arg-Phe amide (FMRFamide)-like neuropeptides by interacting with the channel's large extracellular loop. ASICs are also modulated by G protein-coupled receptors such as CB1 cannabinoid receptors and 5-$HT_2$. This review focuses on the physiological roles of ASICs and the molecular mechanisms by which these channels are regulated.