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http://dx.doi.org/10.5012/bkcs.2004.25.7.997

A New Strategy for Determining Optimum pH of Isozymes  

Yoon, Kil-Joong (Division of Applied Sciences, Cheongju University)
Publication Information
Bulletin of the Korean Chemical Society / v.25, no.7, 2004 , pp. 997-1002 More about this Journal
Abstract
A hydrogenperoxide sensor containing peroxidase extracted from horseradish was constructed and pH effect on its sensing ability was investigated. Current profiles of the biosensor with pH and the electrophoretic analysis showed that horseradish peroxidase consists of two isozymes. Assuming that it is a hypothetical twoisozyme mixture, the current profiles were deconvoluted into two Gaussians. Application of the new Michaelis-Menten equation connoting pH concept to this system enabled to find all the related dissociation constants of the isozyme-substrates and the isozyme-proton complexes and to determine pHs for the maximal isozyme activities.
Keywords
Biosensor; Enzyme electrode; Hydrogen peroxide; Peroxidase; Isozyme;
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