• Biotechnology and Bioprocess Engineering:BBE
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• 제9권2호
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• pp.143-146
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• 2004

A surface plasmon resonance (SPR) imaging system was constructed and used to detect the hexahistidine-ubiquitin-tagged human parathyroid hormone fragment (His$\sub$6/-Ub-hPTHF(1-34)) expressed in Escherichia coli. The hexahistidine-specific antibody was immobilized on a thin gold film coated with ProLinker$\^$TM/ B, a novel calixcrown derivative with a bifunctional coupling property that permits efficient immobilizaton of capture proteins on solid matrices. The soluble and insoluble fractions of an E. coli cell lysate were spotted onto the antibody-coated gold chip, which was then washed with buffer (pH 7.4) solution and dried. SPR imaging measurements were carried out to detect the expressed His$\sub$6/-Ub-hPTHF(1-34). There was no discernible protein image in the uninduced cell lysate, indicating that non-specific binding of contaminant proteins did not occur on the gold chip surface. It is expected that the approach used here to detect affinity-tagged recombinant proteins using an SPR imaging technique could be used as a powerful tool for the analyses of a number of proteins in a high-throughput mode.

• 한국작물학회지
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• 제37권1호
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• pp.86-92
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• 1992

최근들어 우리나라 국민의 식품소비 형태가 점차 고급화됨에 따라 메밀의 어린 식물체와 종실은 건강 별미식품의 원료로서 수요량은 단속 증가될 전망이다. 엽채소용으로 이용되는 메밀의 어린 식물체는 30% 이상의 단백질과 500mg / 100g 이상의 Rutin과 상당한 양의 미네랄, 비타민 함유하여 단경기 재배시 녹채소의 수량은 파종량, 파종기, 재배환경 조건에 따라서 262～2,270 / 10a로 차이가 매우 컸으며 예상조수익도 52.4～183.5만원 / 10a이었다. 엽채소용 메밀의 육묘기간을 20, 25, 30일로 하였을 경우 육묘기간이 길수록 초장, 줄기의 굵기, 잎의 수와 크기가 증대되었다. 4배체품종 신주대메밀의 생체중은 2배체품종 수원재래와 신농001에 비하여 35%가 더 무거웠으며 엽육이 두껍고 잎이 커서 상품가치가 더 좋았다.

• Bulletin of the Korean Chemical Society
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• 제33권10호
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• pp.3269-3273
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• 2012

In this study, we report a new method for the high contrast imaging of biomolecular interactions at roller printed protein surfaces using thermotropic liquid crystals (LCs). Avidin was roller printed and covalently immobilized onto the obliquely deposited gold surface that was decorated with carboxylic acid-terminated self-assembled monolayers (SAMs). The optical response of LCs on the roller printed film of avidin contrasted sharply with that on the obliquely deposited gold surface. The binding of biotin-peroxidase to the roller printed avidin was then investigated on the obliquely deposited gold substrate. LCs exhibited a non-uniform and random orientation on the roller printed area decorated with the complex of avidin and biotin-peroxidase, while LCs displayed a uniform and planar orientation on the area without roller printed proteins. The orientational transition of LCs from uniform to non-uniform state was triggered by the erasion of nanometer-scale topographies on the roller printed surface after the binding of biotin-peroxidase to the surface-immobilized avidin. The specific binding events of protein-receptor interactions were also confirmed by atomic force microscopy and ellipsometry. These results demonstrate that the roller printing of proteins on obliquely deposited gold substrates could provide a high contrast signal for imaging biomolecular interactions using LC-based sensors.

• 한국축산식품학회지
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• 제35권2호
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• pp.216-224
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• 2015

The objective of present study was to evaluate the effects of the application of chitosan and chitosan/whey protein on the chemical, microbial and organoleptic properties of Göbek Kashar cheese during ripening time (on 3^rd, 30^th, 60^th and 90^th d). Difference in microbiological and chemical changes between samples was found to be significant (p<0.05) during ripening period. Cheese samples with edible coating had statistically lower mould counts compared to the uncoated samples. Furthermore the highest and lowest mould counts were determined in control (4.20 Log CFU/g) and other samples (<1 Log CFU/g) at 60^th and 90^th d of storage. All samples exhibited higher levels of water soluble nitrogen and ripening index at the end of storage process. At the end of 90 day storage period, no signicant dierences in salt and fat values were observed among the cheeses studied. The edible coatings had a beneficial effect on the sensory quality of cheese samples. In the result of sensory analysis, while cheese C and the chitosan coated cheese samples were more preferred by the panellists, the chitosan/whey protein film-coated cheese samples received the lowest scores. This study shows coating suggests could be used to improve the quality of cheese during ripening time.

• 멤브레인
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• 제16권1호
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• pp.39-50
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• 2006

실리카 입자를 기공 형성제로 사용하여 다공성 키토산 및 키틴 막을 제조하였다. 다공성 막의 제조는 다음의 3단계 절차로서 수행되었다: (1) 키토산 용액에 실리카 입자를 첨가시켜 필름을 형성시킨 후, (2) 이 필름을 알카리 용액에 침지시켜 실리카 입자를 제거하여 다공성의 키토산 막을 제조하였으며, (3) 다공성 키토산 막을 acetic anhydride를 사용하여 아세틸화시킴으로서 다공성 키틴 막을 제조하였다. 물리적 강도가 우수하고, 적절한 순수 투과량을 갖는 다공성 키토산 막과 키틴 막의 최적 제막조건이 제시되었다. 단백질 친화성을 부여하기 위해 다공성 키토산 막에 반응성 염료인 Cibacron Blue 3GA를 고정화시켰으며, BSA 단백질 및 lysozyme 효소의 흡착실험을 수행하여 친화 키토산 막 및 키틴 막의 단백질 결합용량을 측정하였다. 친화 키토산 막의 BSA 단백질 결합용량은 약 22 mg/mL이었으며, 친화 키틴 막의 lysozyme 효소 결합용량은 약 26 mg/mL로서 이는 키토산 또는 키틴을 기반으로 하여 제조된 hydrogel bead의 단백질 결합용량보다 수${\sim}$수십 배 큰 값으로서, 향후 막여과 크로마토그래피용 친화 막으로의 효과적인 활용이 기대된다.

• Journal of Periodontal and Implant Science
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• 제41권6호
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• pp.263-272
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• 2011

A number of surface modification techniques using immobilization of biofunctional molecules of Titanium (Ti) for dental implants as well as surface properties of Ti and Ti alloys have been developed. The method using passive surface oxide film on titanium takes advantage of the fact that the surface film on Ti consists mainly of amorphous or low-crystalline and nonstoichiometric $TiO_2$. In another method, the reconstruction of passive films, calcium phosphate naturally forms on Ti and its alloys, which is characteristic of Ti. A third method uses the surface active hydroxyl group. The oxide surface immediately reacts with water molecules and hydroxyl groups are formed. The hydroxyl groups dissociate in aqueous solutions and show acidic and basic properties. Several additional methods are also possible, including surface modification techniques, immobilization of poly(ethylene glycol), and immobilization of biomolecules such as bone morphogenetic protein, peptide, collagen, hydrogel, and gelatin.

• 농업과학연구
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• 제11권1호
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• pp.45-52
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• 1984

Changes of chemical componts and populations of total bacteria and lactic acid bacteria were examined during a month-long storage of sweet potatoes crushed and sealed up with polyethylene film at $7-8^{\circ}C$. 1. Changes of starch, total protein, volatile acid and ammonia-nitrogen contents were li 2. In a three days, pH down and increase of non-volatile acid content were notable, populations of total bacteria and lactic acid bacteria were maximum. 3. Vitamic C and soluble sugar contents tended to be reduced during the storage and the leftover were 75-85% and 41-45% respectively. 4. ${\beta}$-Amylase activity decreased gradually and vanished 15-30days. 5. Variations of chemical compontents of the samples inoculated lactic acid bacteria were larger than non- treated to some extent. 6. As above results, mainly homo lactic acid fermentation was done in this storage condition.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.30-33
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• 2002

We have shown that it is possible to form a fibrilar network of fibronectin on a polyelectrolyte polymer film whose dimensions are similar to those reported on the extra cellular matrix. The fibronectin network was observed to form only when the charge density of the polymer was in excess of the natural charge density of the cell wall. Furthermore, the self-organized fibronectin layer was much thicker than the polymer film, indicating that long ranged interaction may playa key role in the assembly process. It is therefore important to understand the structure of the polymer layer/protein interface. Here we report on a neutron reflectivity study where we explore the structure of the polyelectrolyte layer, in this case sulfonated polystyrene (PSSx,), with varying degree of sulfonation (x<30%), as a function of sulfur content and counter ion concentration. These results are then correlated with systemic study of the adsorption and the multilayer formation of fibronectin as a function of incubation time for various sulfonation levels of $PSSx.^1$

• 방재와보험
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• 통권36호
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• pp.31-33
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• 1988

1960년대까지는 저팽창단백포가 유류화재의 진압에 주로 사용되었으니 불소화합물 계면활성제로부터 유출된 합성 AFFF를 개발하면서 해수로도 이의 사용이 가능하고 지역 및 유출화재 예방에서 AFFF 특유의 초기진압효과 및 대형화재를 방지한다는 장점 때문에 널리 사용되고 있으며, 석유화학산업의 저장탱크지역 및 공정지역에서 AFFF와 FPF가 주로 사용되며 부두시설(Oilrig, Marine Tanker, 해상 / 해변터미널)에는 AFFF의 사용이 지배적이다. 현재 사용되고 있는 foam으로는 단백포(Protein: PF), 불화단백포(Fluoroprotein: FPF), 막형성 불화단백포(Film Forming Fluoroprotein Foam: FFFP), 고팽창폼(High Expansion Foam), 수성막 형성폼(Aqueous Film Foaming Foam: AFFF), 알콜폼(Alcohol Resistant Foam: ATD) 등이 있으니 본고에서는 AFFF 중심으로 소개한다.

• 한국식품과학회지
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• 제43권3호
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• pp.321-328
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• 2011

본 연구에서는 대추를 필름 형태의 식품 소재로 개발하였다. 대추 필름 제작에 고분자 분쇄 방법으로 사용된 HPH는 대추 필름의 인장 강도와 수분 투과도를 향상시켰다. 기존에 개발된 많은 다른 생고분자 필름보다 높은 인장 강도와 낮은 수분 투과도는 열접합강도와 함께 식품에 있어 HPH 대추 필름의 상업적 적용 가능성을 보여주었다. 대추 필름은 대추 고유의 형태와 크기에 제한 없이 여러 식품에 코팅 또는 롤 형태로 사용될 수 있을 것으로 기대된다.