• Title/Summary/Keyword: Protein S

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Chemical Components and Antimicrobial Activity of Garlics from Different Cultivated Area (산지별 마늘의 화학성분 및 항균활성)

  • Jeong, Chang-Ho;Bae, Young-Il;Lee, Jin-Hwa;Roh, Jeang-Gwan;Shin, Chang-Sik;Choi, Jine-Shang;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.43 no.1
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    • pp.51-59
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    • 2009
  • The chemical components and antimicrobial activities of garlic from different area were investigated and analyzed to provide basic data for functional food materialization and processing. Hunter's values of garlic from different area were L 53.41~57.15, a -3.49~-4.38 and b 11.47~17.55. The moisture, crude protein, crude fat, nitrogen free extract, crude fiber and ash were 65.24~71.96, 6.24~9.35, 0.21~0.49, 19.01~22.72, 0.58~0.95 and 1.01~2.01%, respectively. The major minerals of garlic from different area were Na(27.22~112.03), Mg(18.17~32.56), K(242.16~569.28), Ca(28.60~63.93), P(117.72~265.21 mg%) and major free sugars were sucrose, glucose and fructose. The major amino acids of garlic from different area were proline, arglmne, glutamic acid and aspartic acid and content of total amino acid was 2,709.33~4,561.04 mg%. The ascorbic acid content of garlic from different area was 2.966~8.673 mg%. Composition of fatty acids of garlic from different area were linoleic acid, oleic acid and palmitic acid, unsaturated fatty acid and saturated fatty acid contents were 72.18~74.35 and 25.65~27.82%, respectively. Antimicrobial activities of garlic extracts as different area increased depends on concentration and showed the high antimicrobial activities against Gram(+) and Gram(-).

Physicochemical Properties of Diverse Rice Species (품종별 쌀의 이화학적 특성)

  • Choi, Ok-Ja;Kim, Yong-Doo;Shim, Jae-Han;Noh, Myeong-Hee;Shim, Ki-Hoon
    • Food Science and Preservation
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    • v.19 no.4
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    • pp.532-538
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    • 2012
  • Seven rice varieties (Dasan, Keunseon, Goami, Baekjinju, Seolgaeng, Hangangchal and Heukseol) were used to study the physicochemical properties of dry milled (200-mesh) rice flour species. The moisture and crude protein contents of rice were 15.00-15.10% and 6.09-8.21%, respectively. The crude lipid and crude ash of rice were 0.21-1.02% and 0.37-1.62%, respectively. As for the Hunter's color value, the L value was highest in the Dasan flour (96.47); the a value was highest in the Heukseol flour (5.03); and the b value was highest in the Baekjinju flour (3.36). The water aborption index was highest in the Goami flour (1.45), and the water solubility index was highest in the Hangangchal flour (9.16%). The amylose contents of the rice flour species were highest in the Goami (26.42%) rice flour, followed by the Dasan (19.39%), Seolgaeng (19.24%), Keunseon (18.06%), Heukseol (15.52%), Baekjinju (9.16%), and Hangangchal (0.84%) rice flour. In the X-raydiffractin patterns of the diverse species, seven tice varieties showed A-type crystallinity. As for the amylogram properties, the initial pasting temperature was 58.00-$69.03^{\circ}C$. The maximum viscosity was highest in the Dasan flour. The Heukseol flour had the lowest maximum viscosity, breakdown, and setback. In terms of the thermal properties of the differential scanning calorimeter (DSC), the onset temperature was 59.03-$66.84^{\circ}C$; the peak temperature, 66-70-$72.82^{\circ}C$; and the end temperature, 74.06-$78.66^{\circ}C$. The enthalpy (${\Delta}H$) was lowest in the Heukseol flour (7.59 J/g) and highest in the Seolgaeng flour (11.36J/g).

Production of Antimicrobial Compounds and Cloning of a dctA Gene Related Uptake of Organic Acids from a Biocontrol Bacterium Pseudomonas Chlororaphis O6 (생물적 방제균 Pseudomonas chlororaphis O6의 길항 물질 생산 및 유기산 흡수에 관련된 dctA 유전자의 클로닝)

  • Han, Song-Hee;Nam, Hyo-Song;Kang, Beom-Ryong;Kim, Kil-Yong;Koo, Bon-Sung;Cho, Baik-Ho;Kim, Young-Cheol
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.3
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    • pp.134-144
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    • 2003
  • A rhizobacterium Pseudomonas cholororaphis O6 produced several secondary metabolites, such as phenazines, protease, and HCN that may be involved in inhibition of the growth of phytopathogenic fungi. In field study, P. chlororaphis O6 treatment on wheat seed suppressed root rot disease caused by Fusarium culmorum. The major organic acids of cucumber root exudates were fumaric acid, malic acid, benzoic acid, and succinic acid. Glucose and fructose were major monosaccharides in cucumber root exudates. The total amount of organic acids was ten times higher than that of the sugars. P. chlororaphis O6 grew well on cucumber root exudates. The dctA gene of P. chlororaphis O6 consisted of a 1,335 bp open reading frame with a deduced amino acid sequence of 444 residues, corresponding to a molecular size of about 47 kD and pI 8.2. The deduced dctA sequence has ten putative transmembrane domains, as expected of a membrane-embedded protein. Our results indicated that organic acids in cucumber root exudates may play an important role in providing nutrient source for root colonization of biological control bacteria, and the dctA gene of P. chlororaphis O6 may be an important bacterial trait that is involved in utilization of root exudates.

A New High Quality and Yielding Barley Variety "Geungangbori" with Lodging Resistance (겉보리 단간 내도복 다수성 일시 출수형 "건강보리")

  • Hyun, Jong-Nae;Kweon, Soon-Jong;Park, Dong-Su;Ko, Jong-Min;Han, Sang-Ik;Lim, Sea-Gye;Suh, Se-Jung
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.474-478
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    • 2008
  • A new covered barley variety, "Geungangbori" was developed from the cross between Milyang 55 which have lodging tolerance and easy brittleness and Suweon 260 with good quality by barley breeding team in the Yeongnam Agricultural Research Institute (YARI) in 2002. A promising line, YMB3855-3B-14-1-1-1, was selected in 1999. It was designated as the name of Milyang 110. It was prominent and had good result from regional adaptation yield trials (RAT) for three years from 2000 to 2002 and released as the name of "Geungangbori". Geungangbori is resistant to barley yellow mosaic virus and moderately resistant to powdery mildow. The average maturing date was same with Olbori on paddy field in regional adaptation yield trials for 2000-2002. Its culm length is 17 cm shorter than that of Olbori and the spike length is 4.4cm, it's longer than olbori. The 1,000 grain weight of Geungangbori was 34 g, same as Olbori, but the number of spikes per $m^2$ and test weight ware lower than those of Olbori. The yield potential of Geungangbori was 4.22 MT/ha on paddy in regional adaptation yield trials for 2000-2002. which was 7% higher than that of Olbori. The cooking quality of Geungangbori were similar to Olbori such as water absorption rate and expansion rate. But the crude protein content is lower than Olbori. This variety is suitable for double cropping system with rice in the southern part of the Korean Peninsula.

A New Early-Heading and High-Yielding Forage Rye Variety, 'Dagreen' (조숙 다수성 청예 조사료용 호밀 신품종 '다그린')

  • Cheong, Young-Keun;Heo, Hwa-Young;Park, Hyoung-Ho;Hwang, Jong-Jin;Han, Ouk-Kyu;Park, Tae-Il;Park, Ki-Hun;Choi, Jae-Seong;Seo, Jae-Hwan;Kim, Dae-Wook;Kim, Ki-Jong;Kim, Jung-Gon
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.600-605
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    • 2010
  • 'Dagreen' (Scale cereal L.), a new rye variety was developed from the open pollination among 10 rye lines at the National Institute of Crop Science in 1995 and mass selection were made at National Institute of Crop Science from 1996 to 1999. Recurrent selections were made at Dept. Rice and Winter Cereal Crop, NICS, RDA from 2000 to 2006. This new variety has erect plant type with medium size pale green leaves. The number of spike per $m^2$ was 713 and the plant height was 103cm. The heading date of 'Dagreen' was April 24 which was 7 days earlier than that of "Koolgrazer". It was adaptable for forage use at an early stage as a whole crop. Lodging resistance was higher than that of check variety 'Koolgrazer'. The chemical components and quality of forage showed 10.2 % crude protein, 36.6 % ADF, 62.4 % NDF and 59.9 % TDN. 'Dagreen' showed high resistance to powdery mildew and leaf rust than those of check variety in the field condition. The average dry matter (7,010kg $ha^{-1}$) of 'Dagreen' harvested at April 28 were 6 % higher than 'Koolgrazer'. This variety is recommended for all of the rye cultivation area in Korea.

Feeding Effects of Whole Crop Rice based TMR on Growth Performance and Carcass Characteristics of Hanwoo Steers (사료용 벼 위주 TMR 급여가 거세 한우의 생장 능력 및 도체 특성에 미치는 영향)

  • Kim, Jong Geun;Zhao, Guoqiang;Liu, Chang;Nan, Wei Sheng;Kim, Hak Jin;Kim, Kyoung Hoon;Ahn, Eok Geun;Min, Hyung-Gyu
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.97-104
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    • 2019
  • This experiment was conducted to investigate the effect of whole crop rice (WCR) based TMR on growth performance and carcass characteristics of Hanwoo steers. WCR "Yeongwoo"was harvested at yellow ripen stage and ensiled for 60 days. The crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), in vitro dry matter digestibility (IVDMD) and total digestible nutrient (TDN) content was 8.4 %, 28.0 %, 53.8 %, 72.4 % and 66.8 %, respectively. For silage quality, pH was 4.37 and lactic and butyric acid content were 2.84 and 0.04 % in DM. Sixteen Hanwoo steers (8-mon-old) were allocated into either a control (commercial TMR) and WCR-TMR (WCR-based TMR) group. The TMR were fed according to the feeding stage phase: growing (Initiate~14 month), early fattening (15 month~21 month) and late fattening (22 month~30 month). The body weight of control group increased (P<0.05) until early fattening stage, but late growing stage of WCR-TMR group was higher than that of control (P<0.05). Average daily gain (ADG) was significantly greater (P<0.05) in WCR-TMR group (total 0.78 kg/head) compared to control (total 0.66 kg/head) except for late fattening stage. The marketing weight and carcass weight were higher in WCR-TMR group (726 vs 765 kg; 417.8 vs 450.4 kg). The back fat thickness (11.75 vs 13.00 mm), Longissimus dorsi area (88.00 vs $89.88cm^2$) and yield index (65.87 vs 64.30) were not different between the two groups (P>0.05) and also no difference in meat yield grade (A : B : C = 2 : 4 : 2). Marbling score (4.00 vs 4.13), meat color (4.75 vs 4.75), fat color (3.13 vs 2.88), texture (1.25 vs 1.50) and maturity (2.00 vs 2.00) were not significant difference between the two groups and meat quality grade ($1^{{+}{+}}:1^+:1:2:3=0:2:4:2:0$) was also not different. In conclusion, TMR feeding based on WCR silage showed superiority in carcass yield and ADG compared to control TMR. It is considered that the use of WCR for feed is a necessary option for the substitution of the imported forages and the government's policy for rice production adjustment.

Comparative Study on the Productivity and Quality of Hilly Pasture by Management type (경영형태별 산지 초지의 생산성 및 사료가치 비교 연구)

  • Kim, Jong Geun;Liu, Chang;Zhao, Guoqiang;Park, Hyung Soo;Jeong, Jong Sung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.1
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    • pp.45-52
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    • 2019
  • This experiment was carried out to study the change of productivity and feed value in different types of hilly pasture. The pasture utilized in the experiment was placed on the experimental farm of Pyeongchang Campus of Seoul National University. Forage production type(FP; Orchardgrass 18 + Tall Fescue 12 + Timothy 5 + White clover 5 kg/ha) and Public farm type(PF: Orchardgrass 12 + Tall Fescue 18 + Timothy 5 + White clover 5 kg/ha) pasture were established in September 3, 2014 and utilized (cutting or grazing) four times every year. Growth characteristics, yield and forage quality were investigated for two years. Plant height of grasses was the highest in the $1^{st}$ cutting and legumes was in the $2^{nd}$ cutting. Dry matter (DM) content was highest at every the $1^{st}$ cut grasses significantly lower at the $2^{nd}$ harvest (p<0.05). However, there was no significant difference in the $2^{nd}$, $3^{rd}$ and $4^{th}$ harvest in 2016. In the botanical composition change, the portion of legume was gradually increased after pasture establishment and the ratio of weed and bare land was higher at $2^{nd}$ and $3^{rd}$ cutting, but it was decreased at $4^{th}$ harvest. There was no significant difference in 2016 of fresh yield between two farm types (p>0.05). The yield of dry matter showed similar trend of fresh yield and forage production type was higher than that of public farm type (p<0.05). The forage intake by livestock was 1,452 kg/ha in 2015 and 1,743 kg/ha in 2016. Pasture utilization ratio of public farm type pasture was highest in the $3^{rd}$ grazing time. Forage quality of pasture in relation to management type had not significant difference, but there was difference in harvest times. Crude protein (CP) was the lowest in the $1^{st}$ harvest and total digestible nutrient (TDN) was highest in the $1^{st}$ harvest and lowest in the $4^{th}$ harvest. Based on the above results, it is found that the establishment of pasture suitable for farm's situation is important for set up of Korean model of hilly pasture. Although the forage production type is superior on forage productivity, it is recommended that the results will be provided as basic data for management of public farm type in the future.

Base Study Related with Development of Natural Bio-Adhesives Using Seaweeds (해초류를 이용한 천연 바이오 접착제 개발 기반 연구)

  • Han, Won-Sik;Oh, Seung-Jun;kim, Young-Mi;Lee, You-Jin;Kim, Ye-Jin;Park, Min-Seon;Wi, Koang-Chul
    • Journal of Conservation Science
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    • v.34 no.6
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    • pp.595-604
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    • 2018
  • In this study, in a bid to develop natural bioadhesives for paper craft, the hanji industry, and preserving cultural assets, complex polysaccharides were extracted from brown and red algae and used as an ingredient in adhesives. Brown algae include sea trumpet, kelp, sea oak, and sea mustard, whereas red algae include Pachymeniopsis elliptica agar-agar weed, Gloiopeltis tenax, and hunori. The polysaccharides were extracted after transforming them from non-aqueous Ca complexes contained in each of the brown and red algae into water-soluble polysaccharides containing alkali metals with a solubility level of 1. and extracted Subsequently, only the polysaccharides were extracted using alcohol precipitation. The adhesion tensile strengths of kelp, a brown algae, and Pachymeniopsis elliptica, a red algae, were 21.58 and 32.99 kgf, respectively. They thus demonstrated better adhesion than that of solid glue products such as water plants (18.45 kgf) and glue sticks (20.45 kgf). The extraction yield of these polysaccharides is supposed to be determined according to their extracted environments; however, no difference in adhesion strength was seen. Further, it was found that the shapes of polysaccharides were determined by their growing environment instead of extraction environment. Use of multi-step alcohol precipitation method during extraction enabled the removal of the constituents except protein and other polysaccharides, thereby demonstrating a stable outcome without cultivation of mold. Furthermore, there was no occurrence of mold even after production of the adhesives by the simple solution method, which demonstrates the adhesive's potential as an environment-friendly adhesive material.

A New Forage Barley (Hordeum vulgare L.) Cultivar 'Mihan' with Ruminant-Palatable Semi-Smooth Awn (가축 기호성이 좋은 반매끈망을 가진 청보리 신품종 '미한')

  • Park, Jong-Ho;Cheong, Young-Keun;Kim, Kyong-Ho;Park, Tae-Il;Kim, Yang-Kil;Park, Hyoung-Ho;Yoon, Young-Mi;Han, Ouk-Kyu;Yun, Geon-Sig;Hong, Ki-Heung;Bae, Jeong-Suk;Song, Jae-Ki;Oh, Young-Jin
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.4
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    • pp.253-259
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    • 2018
  • A new barley (Hordeum vulgare L.) cultivar 'Mihan' having ruminant-palatable semi-smooth awn and good silage quality was developed at National Institute of Crop Science(NICS), RDA in 2016. This cultivar was derived from a cross of the 'SB00T2064' and 'Suwon385' in 2001. And its promising line showed high yielding through the preliminary yield trials(PYT) and advanced yield trials (AYT) in Iksan from 2012 to 2013. It was designated as the 'Iksan487'. 'Iksan487' was conducted to regional yield trials (RYT) in one upland field and five paddy fields around Korea for three years from 2014 to 2016. And it was released as the name of 'Mihan'. It has growth habit of IV, erect plant type, green leaf and semi-smooth awn. Its heading date was April 23 in the paddy field and maturing date was May 24. Plant height of 'Mihan' was 96cm. Mihan's spikes per $m^2$ was 665. It has better winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus) than that of barley cultivar 'Youngyang'. The average dry matter of 'Mihan' was about $10.9ton\;ha^{-1}$ in paddy field. And average feed quality of 'Mihan' was 10.3% of crude protein content, 26.1% of ADF (Acid Detergent Fiber), 46.9 % of NDF (Neutral Detergent Fiber) and 68.2% of TDN (Total Digestible Nutrients). 'Mihan' had grade I of silage quality. This cultivar would be suitable for the area above the daily minimum average temperature of $-8^{\circ}C$ in January in Korean peninsula.

Two new triterpenoid saponins derived from the leaves of Panax ginseng and their antiinflammatory activity

  • Li, Fu;Cao, Yufeng;Luo, Yanyan;Liu, Tingwu;Yan, Guilong;Chen, Liang;Ji, Lilian;Wang, Lun;Chen, Bin;Yaseen, Aftab;Khan, Ashfaq A.;Zhang, Guolin;Jiang, Yunyao;Liu, Jianxun;Wang, Gongcheng;Wang, Ming-Kui;Hu, Weicheng
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.600-605
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    • 2019
  • Background: The leaves and roots of Panax ginseng are rich in ginsenosides. However, the chemical compositions of the leaves and roots of P. ginseng differ, resulting in different medicinal functions. In recent years, the aerial parts of members of the Panax genus have received great attention from natural product chemists as producers of bioactive ginsenosides. The aim of this study was the isolation and structural elucidation of novel, minor ginsenosides in the leaves of P. ginseng and evaluation of their antiinflammatory activity in vitro. Methods: Various chromatographic techniques were applied to obtain pure individual compounds, and their structures were determined by nuclear magnetic resonance and high-resolution mass spectrometry, as well as chemical methods. The antiinflammatory effect of the new compounds was evaluated on lipopolysaccharide-stimulated RAW 264.7 cells. Results and conclusions: Two novel, minor triterpenoid saponins, ginsenoside $LS_1$ (1) and 5,6-didehydroginsenoside $Rg_3$ (2), were isolated from the leaves of P. ginseng. The isolated compounds 1 and 2 were assayed for their inhibitory effect on nitric oxide production in LPS-stimulated RAW 264.7 cells, and Compound 2 showed a significant inhibitory effect with $IC_{50}$ of $37.38{\mu}M$ compared with that of NG-monomethyl-L-arginine ($IC_{50}=90.76{\mu}M$). Moreover, Compound 2 significantly decreased secretion of cytokines such as prostaglandin $E_2$ and tumor necrosis factor-${\alpha}$. In addition, Compound 2 significantly suppressed protein expression of inducible nitric oxide synthase and cyclooxygenase-2. These results suggested that Compound 2 could be used as a valuable candidate for medicinal use or functional food, and the mechanism is warranted for further exploration.