• Macromolecular Research
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• v.11 no.4
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• pp.241-249
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• 2003

A molecular-thermodynamic model to describe the salt-induced protein precipitation is developed based on the perturbation theory. We employed the modified perturbed hard-sphere-chain (PHSC) equation of state for copolymer mixtures to take into account the pre-aggregation effect among protein particles. Hypothetical pressure-composition diagrams are computed with various size differences and salt concentrations. The precipitation behaviors are also studied for various types of pre-aggregation effect for the given systems.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.215-222
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• 1994

This experiment was conducted to find out the response of orchardgrass (Dactylis glorneruta L.) varieties to grazing at the condition of monocultures and mixed pastures on the field of Livestock Experiment Station at Suwon from Sep. 1989 to Oct. 1991. The varieties of orchardgrass used in this experiment were Potomac, Hallmark and Syn. No. 2 as a early maturing type, and Lidacta, Rancho and Syn. No. 10 as a late maturing type. The results obtained were as follows: 1. Herbage utilization of mixture with Hallmark variety(T2) was the highest as 78.2% and that with Rancho variety(T5) was the lowest as 70.2%. but there was no difference(P>0.05). Mixtures with early maturing varieties(T1, T2 and T3) were higher as 77.4% than those with late varieties(T4, T5 and T6) as 72.9% in herbage utilization. In monocultures Rancho variety(T5) was the highest as 68.6% and Lidacta variety(T4) was the lowest as 58.8%, and mixtures with early maturing varieties(T1, T2 and T3) as 66.6% were higher than those of late(T1, 72 and 73) as 64.6%. 2. The content of crude protein of 6 mixed pastures ranged from 19.7% to 22.8%, and those of ether extracts, crude fiber, crude ash and nitrogen free extract were 6.6 to 7.5%, 24.9 to 26.2'76, 1 1.5 to 1 1.9% and 33.6-35.4 8, respectively, But there were not different significantly(P>0.05). In monocultures, significant difference by variety was observed in crude protein content(P<0.05). 3. In botanical composition, there was just a little different among 6 mixtures, having 90% or more in grass percentage. The percentages of each orchardgrass varieties were decreased, but those of perennial ryegrasses were increased as grazing sesson was progressed in all treatment.

• Journal of Ginseng Research
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• v.46 no.1
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• pp.126-137
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• 2022

Background: Nonalcoholic steatohepatitis (NASH) is one of the main chronic liver diseases. NASH is identified by lipid accumulation, inflammation, and fibrosis. Jinan Red Ginseng (JRG) and licorice have been widely used because of their anti-inflammatory and hepatoprotective effects. Hence, this study assessed JRG and licorice extract mixtures' effects on NASH progression. Methods: Palmitic acid (PA) and the western diet (WD) plus, high glucose-fructose water were used to induce in vitro and in vivo NASH. Mice were orally administered with JRG-single (JRG-S) and JRG-mixtures (JRG-M; JRG-S + licorice) at 0, 50, 100, 200 or 400 mg/kg/day once a day during the last half-period of diet feeding. Results: JRG-S and JRG-M reduced NASH-related pathologies in WD-fed mice. JRG-S and JRG-M consistently decreased the mRNA level of genes related with inflammation, fibrosis, and lipid metabolism. The treatment of JRG-S and JRG-M also diminished the SREBP-1c protein levels and the p-AMPK/AMPK ratio. The FAS protein levels were decreased by JRG-M treatment both in vivo and in vitro but not JRG-S. Conclusion: JRG-M effectively reduced lipogenesis by modulating AMPK downstream signaling. Our findings suggest that this mixture can be used as a prophylactic or therapeutic alternative for the remedy of NASH.

• Journal of Nutrition and Health
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• v.29 no.8
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• pp.861-866
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• 1996

Tannins in plant foods and beverages may produce antinutritional or toxic effects although some proteins with high affinity for tannins seem to function as defense mechanism to tannin toxicity. Our objectives were to investigate of tea tannins, iron and protein and to evaluate the role of proteins in tannin effects on iron solubility. Iron solubility in vitro was measured using tea with and without proteins. Mixtures of tea, protein in varying concentrations(either gelatin or bovine serum albumin), and iron(eithe 10 or 50ug/mL) were prepared. Controls contained water in place of tea. Iron bioavailability was assessed by measuring iron solubility in the simulated gastric condition with pepsin digestion. Bound iron was removed by centrifugation and soluble in tea alone. When iron concentratin was 10ug/mL, addition of small amounts of protein to tea dramatically reduced iron solubility, but solubility of iron increased in the tea mixturea as the concentration of protein was increased. The percnetage of iron that precipitated was much greater at 10ug Fe/mL than the values at 50ug Fe/mL suggesting that the iron binding sites on the tea-protein complex was saturated. These results suggest that interactions of iron with tea tannins are influenced by the concentratins of protein and iron.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.517-524
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• 1990

The solubilization and desolubilization of proteins in CTAB/hexanol/isooctane reverse micellar system were investigated for the selective separation of proteins. Several proteins were used, including bovine serum albumin (BSA), pepsin, trysin and ribonuclease-a. Most proteins could be solubilized into reverse micelles in the pH range above the isoelectric point of each protein, where the net charge of protein was opposite to that of surfactant. However BSA was solubilized above pH 10, which is serveral pH units above the pI 4.9. The kinds of anions in aqueous phase influenced on protein solubilization while no significant trend was observed with different cations, Protein solubilization decreased with increase of the ion size in the order of F -, C1-, Br- and I -. The size of CTAB micelles did not change significantly with increasing ionic strength, but the solubilization decreased. Protein desolubilization showedropposite behaviors to the solubilization. Several model mixtures such as pepsin/ trypsin, pepsin/ribonuclease-a and BSAlribonucleaee-a were successfully separated from each other without changing enzymatic activities.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.96-101
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• 1990

This field experiment was undertaken to find out the effect of grazing and cutting management on the forage quality and soil hardness at different legumes sown swards of the experimental field of Livestock Experiment Station in Suweon, from August 1985 to October 1988. The results obtained are as follows: 1. Crude protein, crude fat and ash contents under grazing were higher than these under cutting, but showed the reverse results in crude fiber and nitrogen free extracts contents. Average in vitro dry matter digestibility (IVDMD) under grazing (72 %) was a little higher than that under cutting (71 %). On the other hand, there was little difference among the mixtures swards in mineral contents and IVDMD. 2. The contents of Ca, P and Mg were a little high in the cutting, but K and Na contents were in gazing. Ca: P ratio in grazing (2.97) was slightly higher than that of cutting (2.94). 3. Production of energies(TDN, StE and NEL) with cutting were appeard to increase by 19.4 %, 13.5 %and 19.0 % than those of grazing, respectively. Mixtures swards including alfalfa showed more production both grazing and cutting. 4. Soil hardness in the top lOcm of soil by cattle grazing was changed from 15.5mm at startihg year to 22.6 mm after 3 years, but in cutting slightly increase from 15.5mm to 16.2mm. Generally it was low in early spring and high in autumn.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.245-252
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• 2009

The grassland establishment under the forest become important, but it comes with a shade for the grass growing among trees. The objective of this study is to select the proper grass-clover mixtures to several shading degrees. The grass-clover mixtures were grown under full sunlights and shade cloths, which reduce sunlight intensity by 35, 50 and 75%. The various types of mixtures were allocated at each shading degrees with three replications. The productivity of mixtures was the highest under full sunlights, and intermediate with 35% degree of shading and least with both 50 and 75% degrees of shading. The 1st mixture (orchardgrass+tall fescue+Kentucky bluegrass+ladino clover+perennial ryegrass+timothy) showed the greatest yield in all treatments. The crude protein (CP) concentration of all mixtures increased with the higher degree of shading, but no CP concentration difference was observed in mixtures within same degree of shading. The lowest neutral detergent fiber (NDF) and acid detergent fiber (ADF) concentrations and highest relative feed value (RFV) were resulted in the 1st mixture in all treatments. This study indicates that tall fescue, Kentucky bluegrass, ladino clover, perennial ryegrass and timothy can be used as components for mixtures with orchardgrass which is major component for forest-pastures.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.12
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• pp.1754-1759
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• 2002

A study was carried out to investigate the nutritive value and utilization of hybrid sorghum and perennial grass species viz. setaria (Setaria anceps) and hybrid napier when intercropped with soybean by growing Jersey crossbred heifers. Fifteen growing crossbred heifers (Jersey${\times}$Red Sindhi) of between 7-10 months age and pre-trial average body weight of 49-50 kg were divided on the basis of weight in to three treatment groups viz. $T_1$-hybrid sorghum+soybean, $T_2$-setaria+soybean and $T_3$-hybrid napier+soybean in a completely randomized block design. Intercropped forages were harvested fresh, chaffed and mixed before they were offered to the heifers. Chemical composition of the herbage, dry matter intake (DMI), body weight gain and nutrient digestibility co-efficients were estimated. The herbage mixtures had crude protein (CP) content in the range of 11.87 to 13.86% and ether extract (EE) contents were 2.91 to 3.11%, respectively. The herbage mixtures were rich in minerals (ash). The gross energy (kcal/g DM) was higher in hybrid napier+soybean, while hybrid sorghum+soybean and setaria+soybean herbage mixtures had lower value for gross energy. The hybrid sorghum+soybean and setaria+soybean herbage mixtures had higher contents of NDF, ADF, cellulose, lignin and silica as compared to that of hybrid napier+soybean herbage mixture. The heifers fed hybrid napier+soybean herbage mixture had significantly (p<0.05) higher $DMI\;g/kg\;W^{0.75}$ ($97.41{\pm}4.34$) as compared to hybrid sorghum+soybean ($88.31{\pm}2.66$) and setaria+soybean ($79.29{\pm}1.06$) herbage mixtures. Nutrients digestibility, DCP percent, DCP intake and nitrogen balance were significantly (p<0.05) higher in the heifers fed on hybrid napier+soybean herbage mixture. There was a significant (p<0.05) difference among different herbage mixtures in TDN. The heifers on setaria+soybean herbage mixture had lower average body weight gain (g/day) than those on hybrid sorghum+soybean and hybrid napier+soybean herbage mixtures. Data obtained in this experiment demonstrated that herbage mixture of hybrid napier+soybean was better than hybrid sorghum+soybean and setaria+soybean herbage mixtures in the nutrition of growing heifers. It had highest nutritive value, better digestibility co-efficients which showed better growth rate and higher feed efficiency. In ranking, hybrid napier+soybean herbage mixture was better followed by hybrid sorghum+soybean and setaria+soybean in nutritive value in the parameters studied. For future wasteland development program in humid-sub tropics of Himachal Pradesh hybrid napier and its intercropping with soybean is recommended for general adoption because of its better adaptability and higher nutritive value.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.6
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• pp.1102-1108
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• 1997

Proteins and polysaccharides confer distinct functional properties in food systems. This research was attempted to improve emulsion properties of casein by protein-polysaccharide conjugation, in which alginates with various molecular weights were employed as polysaccharide sources. Casein-alginate mixtures were conjugated by the amino-carbonyl or Maillard reaction at 6$0^{\circ}C$ and 79% relative humidity. The resulting casein-alginate conjugates were tested for their emulsion activity and emulsion stabilizing properties. In general, the emulsion stability of casein-alginate mixture greatly increased due to the amino-carbonyl reaction between casein and alginates, whose magnitude depended on the molecular weight of alginate, weight ratio of casein to alginate and incubation time. It was also found that thermal stability and pH stability were markedly improved by the casein-alginate conjugation.

• Archives of Pharmacal Research
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• v.19 no.4
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• pp.269-274
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• 1996

The binding interactions between human serum albumin (HSA) and the edible food dyes amaranth, tartrazine and sunset yellow have been studied. Intrinsic association constants and the free energy changes associated with dye-protein binding at physiological pH for amaranth and tartrazine, and at two different pH values for sunset yellow have been calculated from ultrafiltration data. The temperature dependence $(20-40^{\circ}C)$ of the intrinsic association constants at pH 7.4 for amaranth-HSA and tartrazine-HSA mixtures have been measured, from which a plot of the van't Hoff isochore exhibits a marked change in slope around $30^{\circ}C$ indicating a possible change in protein conformation. The number of dye binding sites on HSA is reported for all the above conditions. HSA-ligand binding enthalpies have been used in conjunction with the N-B transitional binding enthalpy for HSA, to calculate the enthalpy for the N-B transition when ligands are bound with the protein.