• Title/Summary/Keyword: Protein/Carbohydrate

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Inhibition of Helicobacter pylori Adhesion by Acidic Polysaccharide Isolated from Artemisia capillaris

  • Woo, Jeung-S.;Ha, Byung-H.;Kim, Tae-G.;Lim, Yoon-Gho;Kim, Kyung-H.
    • Journal of Microbiology and Biotechnology
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    • v.13 no.6
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    • pp.853-858
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    • 2003
  • Helicobacter pylori specifically adhere to host cells through a number of putative receptors and ligands, mainly based on carbohydrate-protein interactions. Polysaccharide fractions isolated from the leaves of Artemisia capillaris showed different inhibitory activities against H. pylori adhesion by using hemagglutination assay. Among these fractions, an acidic polysaccharide fraction FlA showed highly effective inhibitory activity, and its minimum inhibition concentration was 0.63 mg/ml. The inhibition results by the hemagglutination assay were consistent with those obtained by the enzymelinked glycosorbent assay, which was developed by the conjugation of horseradish peroxidase with fetuin, a sialic acid-containing glycoprotein which was specific to H. pylori adhesion. FlA contained the highest carbohydrate content among polysaccharide fractions, and no protein was detectable when further purified by gel filtration FPLC. Sugar composition analysis using GC revealed the highest amount of galacturonic acid among sugars, which suggests that FlA contains essentially acidic polysaccharides. Our data suggest that acidic polysaccharides may play an important role in the inhibition of H. pylori adhesion to host cells.

Studies on Self-Selection of 3 macronutrients and the Effect of Electric Stress on Food Selection in Male Rats (3대 열량소를 스스로 선택하게 했을 때 흰쥐의 식이 선택성향 및 저전류 Stress가 이에 미치는 영향)

  • 장영애
    • Journal of Nutrition and Health
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    • v.23 no.7
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    • pp.504-512
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    • 1990
  • In experiment 1, dietary self-selection of the 3 macronutrients, protein, fat, and carbohydrate were examined in male rats given 3 food cups of 80% carbohydrate, 80% protein, and 70% fat diets simultaneously. All the rats showed normal growth pattern and organ weight, which means they have ability to select just right kinds and amounts of nurients in order to support their growth and development. Mean values of caloric intake, body weight gain, serum lipid values and empty carcass compositions were not significantly differ between the upper and lower quartile groups of fat proportion of empty carcass compared to the lower quartile group(LF). Same feeding design was employed in experiment 2 where the effect of mild electric stress on food selection was studied. The rats in both control and electric stress group revealed a normal growth curve and organ weights. The rats in both control and electric stress group revealed a normal growth curve and organ weights. The stress group showed higher caloric intake and body weight gain than control group, but no significant effects of stress on serum and empty carcass components was found. Even though normal rats seemed to select macronutrients according to their physiolosical needs, there were individual differences in food selection whether they were exposed to stress or not. Therefore life long individual food selection pattern may have a great influence on nutritional status and chronic degenerative diseases of eldery, and on aging process.

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A Follow-up Study on the Nutritional Status of Korean Fatty Liver Patients with Different Conditions (한국인 지방간 환자의 병세변화에 따른 영양상태에 관한 Follow-up연구)

  • 김미경
    • Journal of Nutrition and Health
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    • v.26 no.9
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    • pp.1049-1070
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    • 1993
  • The purpose of this study is to investigate dietary factors which influence the conditions of Korean fatty liver patients. The subjects were 83 fatty liver patients living in city, ages of 10 to 60 yrs old, and they were the same patients studied 9 months ago in previous study. The patients were groupsed into 3 groups(improved, similar, worsened groups) according to the changes in disease condition. Nutrient and alcohol intakes and health status were investigated. In improved group, the patients tended to lose weight, the number of exercising patients was increased, the consumptions of calorie, carbohydrate, calcium, phosphorus were decreased. And alcohol intake was decreased markedly and serum total amino acids(AA), EAA, NEAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios were increased. In worsened group, patients tended to gain weight, the number of exercising patients decreased, the consumptions of calorie, protein, carbohydrate, calcium, phosphorus and niacin decreased but vitamin. A intake increased. And also, in this group, alcohol intake of male patients decreased and that of female slightly increased, and serum EAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios, and total fatty acid contents increased and total AA and NEAA contents decreased. In conclusion, it is desirable to reduced weight, stress, alcohol, salt and animal fat consumptions, and to exercise, and to take adequate amount and quality of protein to improve conditions of fatty liver patients.

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A Study on the Nutritional Status of Lunch-box of Highschool Students in a Korean Rural Area (농촌 지역 남.여 고등학생의 도시락 영양실태에 대한 연구)

  • 조희숙
    • The Korean Journal of Food And Nutrition
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    • v.10 no.3
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    • pp.387-393
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    • 1997
  • This study was aimed to investigate the nutritional status of 270 students of high school from September 5 to 10 in 1995, 재 are eating their lunch-box. This results were summarized as follows : The average height of boys and girls was 170.0$\pm$0.8cm and 155.8$\pm$1.4cm, respectively. The average weights of them were 60.5$\pm$0.3kg(male) and 48.5$\pm$0.7kg(female). BMI(Body Mass Index) of them were 20.15$\pm$0.13(male), 17.75$\pm$0.29(female). The weight of the staple of their lunch-box is 301~350g(38.7%, boys) and 200~250g(50.8%, girls). Among the subjects, 54.1% carry two dishes, and they eat 40 kind of side dishes, including kimchi usually cooked by roasting. The balanced diet based on the five basic food groups is almost impossible, especially they don't eat sufficiently protein food and calcium food. All the nutrients except vitamin A, C and niacin, are below the Recommended Daily Dietary Allowances for Koreans. The rate of calorie of carbohydrate : fat : protein is 81.5 : 9.5 : 11.5(boys) and 80.5 : 9.8 : 10.5(girls). The 78.0% of total amount of calorie was from carbohydrate.

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Studies on the Nutritional Status of Lunch-box of Middle Students in a Korean Rural-Sea Area (농어촌 지역 중학생 도시락의 영양 실태에 대한 조사 연구)

  • 조희숙
    • Korean journal of food and cookery science
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    • v.12 no.1
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    • pp.27-33
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    • 1996
  • This study was examined the nutritional conditions of 250 students of middle school from March 5 to 8 in 1993, who are eating their lunch-box. This results were summarized as follows; The average height of the boy students is 156.9 cm. It is 98.6% of the standard height of middle school students. That of the girl students is 153.1 cm. It is 98.7% of the standard height of middle school students. The average weight of the boys is 49.3 kg (98.6% of the standard) and that of the girls is 45.9 kg (95.6% of the standard). The average weight of the staple of their lunch-box is 480 g (boys) and 349.5 g (girls). Among the subjects, 58.8% carry two side dishes, and they eat 36 kinds of side dishes, including kimchi usually cooked by roasting. The balanced diet based on the five basic food groups is almost impossible, especially they don't eat sufficiently protein food and calcium food. All the nutrients except Vitamin A, C and Niacin, are below the Recommended Daily Dietary Allowances for Koreans. The rate of calorie of carbohydrate: fat: protein is 80.5:8. 4:11.1 (boys) and 79.3:9.9:10.8 (girls). The 80% of total amount of calorie was from carbohydrate.

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Development of Functional Yogurts Prepared with Mulberries and Mulberry Tree Leaves

  • Lee, An-Cheol;Hong, Youn-Ho
    • Food Science of Animal Resources
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    • v.30 no.4
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    • pp.649-654
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    • 2010
  • In order to develop new functional yogurts using mulberries and mulberry leaves, which were cultivated in Hwasun-gun, Jeonnam Province, Korea, the nutritional compositions, fermentation conditions, sensory properties, and storage stabilities of the yogurts were analyzed. The mulberry powder yogurt contained 87.96% moisture, 3.21% carbohydrate, 4.52% protein, 3.63% lipid, and 0.68% ash, and the mulberry leaf yogurt contained 86.36% moisture, 4.13% carbohydrate, 4.87% protein, 3.79% lipid, and 0.85% ash. A yogurt base was fermented for 13 h with 0.01% ABT-5 starter inoculum at $40^{\circ}C$. To prepare the mulberry jam and mulberry leaf yogurts, a variety of mulberry jam and mulberry leaf samples were added to the yogurt base. The sensory evaluation results of the yogurts containing the mulberry jam and mulberry leaves indicated that a product made with 15% mulberry jam was more strongly preferred than other samples. When the mulberry jam and mulberry leaf yogurts were stored at $4^{\circ}C$ for 15 d, there were no significant changes in pH, titratable acidity, or viable cell numbers of lactic acid bacteria and Bifidobacterium bifidum.

A Study on the Eating Behavior, Nutritional Status and Health Condition of Obese Adult Attending a Weight Control Exercise (체중조절 운동을 하고 있는 비만 성인의 식사행동과 영양 섭취 상태 및 건강상태에 관한 연구)

  • 이영순
    • The Korean Journal of Food And Nutrition
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    • v.13 no.2
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    • pp.125-133
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    • 2000
  • The purpose of this study was to investigate eating behavior, nutritional status and health condition of obese adult attending a weight control exercise. The subjects of this study consisted of 81 adult in some areas of Daegu. Their weight, height, triceps, midarm circumference were measured and their dietary intake and eating behavior were obtained by using questionnaires. Anthropometry was conducted to determine obesity. Average age of men and women were 38.1 and 35.2 years old respectively. Their intake of energy and protein were 69.5% and 97.4% of RDA respectively. Energy intake of normal and obese group was 74.4% and 64.9% of RDA respectively. Protein intake of normal and obese group was 99.7% and 95.3% of RDA respectively There was a negative correlation between PIBW and energy intake, PIBW and carbohydrate intake, BMI and energy intake, and BMI and carbohydrate intake. A relative magnitude of factors affecting weight decrease rate was analyzed by Stepwise multiple regression analysis. Overall results about relative influence of independent variables to dependent variable(weight decrease rate) indicated that the BMI(p<0.01) was the most significantly correlated with weight decrease rate in all subjects. The results of this study suggest that the extensive nutrition education in weight control program should be emphasized to prevent obesity early.

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Characteristics of Nutrient Intake According to Metabolic Syndrome in Korean Elderly - Using Data from the Korea National Health and Nutrition Examination Survey 2010 - (노인의 대사증후군 유병에 따른 영양소 섭취 특성 - 국민건강영양조사 제5기 1차년도(2010) 자료를 이용하여 -)

  • Kim, Mi Hyun
    • The Korean Journal of Food And Nutrition
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    • v.26 no.3
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    • pp.515-525
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    • 2013
  • This study was performed to investigate the characteristics of nutrient intake according to metabolic syndrome in Korean elderly. Data on 1,200 elderly (men 545, women 655) over 65 years was obtained from the Korea National Health and Nutrition Examination Survey 2010. The subjects were divided into metabolic syndrome (MetS) and normal groups based on NCEP-ATP III guidelines and by applying the Asia-Pacific definition with waist circumference. The prevalence of MetS was 48.9% in all subjects, 35.6% in men and 58.9% in women, respectively. All nutrient intake was significantly lower in MetS than normal subjects, except iron, vitamin A, carotene, and retinol. In the carbohydrate: protein: fat (C:P:F) ratio, MetS showed a higher rate for carbohydrate, but a lower rate for protein and fat than normal. MetS was lower in calcium, vitamin B1, and vitamin C by the nutrient adequacy ratio than normal. Mean adequacy ratio of MetS (0.69) was lower than that of the normal group (0.72). These results demonstrated that undernutrition, not excess intake of specific nutrients - energy, fat, or cholesterol was associated with the risk for MetS in Korean elderly with MetS. Therefore, it is recommended to have a healthy dietary pattern with diverse nutrients for the prevention of MetS.

Proximate Chemical Composition and Endogenous Gibberellins of Chufa (Cyperus esculentus L.) in Upland and Wetland

  • Kim, Sang-Kuk;Choi, Hong-Jib;Kang, Sang-Mo;Lee, In-Jung;Kim, Hak-Yoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.94-98
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    • 2011
  • Proximate chemical components (protein, oil, carbohydrate, ash, fiber, and starch) were determined from tubers grown in upland and wetland conditions. The contents of crude protein, oil, carbohydrate, and starch were higher in upland condition than in wetland condition. Eight gibberellins were commonly identified and quantified in leaves and tuber of chufa grown in wetland and upland field during growing season. Gibberellin content was always higher specifically in the leaves and tubers grown in wetland condition than in those grown in upland condition. The current knowledge of gibberellin biosynthesis suggests that the two endogenous bio-active gibberellins both $GA_1$ and $GA_4$ are differently metabolized according to cultural conditions. Major gibberellin biosynthesis route is ascertained dominantly the non C-13 hydroxylation pathway leading $GA_4$ in chufa plants.

Aging (II): Analysis of Pathway among Factors Affecting Food Intake and Cognitive Function of the Aged (노화 (II): 노인의 식품섭취와 인지기능에 영향을 주는 요인들간의 경로분석)

  • Kang, Hae-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.17 no.3
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    • pp.289-298
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    • 2002
  • This study was conducted to find sensitive indicator of food intake and specific food group related to cognitive function and to analyze the pathway among factors affecting food intake and cognitive function of the aged. 179 people over 50 years old were investigated and all the data were analyzed by SPSS 10.0 package. Food frequency was more sensitive indicator to show the relationship between food intake and cognitive function of the aged than the numbers of standard amount of food eaten. Consumption of grains and starch, meat, fish, eggs and beans were strongly related to cognitive function of the aged and intake of vegetables and fruits also have relationship with it. Moreover, the more frequent eating the carbohydrate and the protein, the higher the cognitive level of the aged. Age, education period, income, blood pressure, duration after retirement, frequency of physical activity and BMI were correlated with food intake and cognitive function of the aged. Among them, age and frequency of physical activity were most strongly correlated with food intake, and education period with cognitive function of them. This investigation shows that more stable intake of carbohydrate and protein containing foods need to be supplied to low income aged and the importance of physical activity of the aged must be emphasized with food intake for better life of the aged.